Study of Different Variants of Mo Enzyme crARC and the Interaction
... activity. It is known that the activity under aerobic conditions of most Mo Cofactor proteins is very low, and usually after a few hours they start to lose their activity [22]. Accordingly, we have studied the stability of crARC activity at 22 and 4 ◦ C by determining its NHC reduction activity at d ...
... activity. It is known that the activity under aerobic conditions of most Mo Cofactor proteins is very low, and usually after a few hours they start to lose their activity [22]. Accordingly, we have studied the stability of crARC activity at 22 and 4 ◦ C by determining its NHC reduction activity at d ...
Identification of enzymes involved in anaerobic benzene
... the culture was transferred from benzene to a different substrate, the community structure of benzene-, phenoland benzoate-grown cultures was assessed by T-RFLP analysis when approximately 25 mM ferrous iron was produced (Fig. S1). With all substrates, the analysis showed the same dominance of the P ...
... the culture was transferred from benzene to a different substrate, the community structure of benzene-, phenoland benzoate-grown cultures was assessed by T-RFLP analysis when approximately 25 mM ferrous iron was produced (Fig. S1). With all substrates, the analysis showed the same dominance of the P ...
Postexercise nutrient intake timing in humans is critical to recovery
... intake after exercise has been demonstrated, little information exists as to the influence of timing for postexercise protein intake. Exercise induces a greater sensitivity and responsiveness to the events controlled by insulin (44). In vitro and in vivo studies have demonstrated increased insulin-m ...
... intake after exercise has been demonstrated, little information exists as to the influence of timing for postexercise protein intake. Exercise induces a greater sensitivity and responsiveness to the events controlled by insulin (44). In vitro and in vivo studies have demonstrated increased insulin-m ...
The Terminal Enzymes of Sialic Acid Metabolism: Acylneuraminate
... The acylneuraminate pyruvate-lyase gene from Clostridium perfringens was sequenced and found to be most similar to the lyase gene from Haemophilus influenzae. Both the recombinant clostridial enzyme and the native enzyme from pig kidney were purified in larger amounts and characterized. The properti ...
... The acylneuraminate pyruvate-lyase gene from Clostridium perfringens was sequenced and found to be most similar to the lyase gene from Haemophilus influenzae. Both the recombinant clostridial enzyme and the native enzyme from pig kidney were purified in larger amounts and characterized. The properti ...
The integrity of a cholesterol-binding pocket in Niemann–Pick C2
... and crystal structure, as well as identification of cavities, was done by using the SWISS-PDB VIEWER (18). Results Purification of Functional Tagged NPC2 Protein and Stoichiometry of NPC2 Action. The novelty of the NPC2 protein and the lack of any ...
... and crystal structure, as well as identification of cavities, was done by using the SWISS-PDB VIEWER (18). Results Purification of Functional Tagged NPC2 Protein and Stoichiometry of NPC2 Action. The novelty of the NPC2 protein and the lack of any ...
Short-Chain-Length Polyhydroxyalkanoates: Synthesis in
... 3-, 4-, 5-, or 6-carbon position with various side groups have been found to be incorporated into PHAs [55]. Since PHAs exhibit biodegradable/biocompatible plastic and elastomeric properties, depending on the monomer constituents, their commercial applications have been extensively studied. Accordin ...
... 3-, 4-, 5-, or 6-carbon position with various side groups have been found to be incorporated into PHAs [55]. Since PHAs exhibit biodegradable/biocompatible plastic and elastomeric properties, depending on the monomer constituents, their commercial applications have been extensively studied. Accordin ...
Safety assessment - Food Standards Australia New Zealand
... information to consumers. In fulfilling these objectives, FSANZ also had regard for the need for standards to be based on risk analysis using the best available scientific evidence and the desirability of an efficient and internationally competitive food industry. ...
... information to consumers. In fulfilling these objectives, FSANZ also had regard for the need for standards to be based on risk analysis using the best available scientific evidence and the desirability of an efficient and internationally competitive food industry. ...
Anchoring of Surface Proteins to the Cell Wall of Staphylococcus
... all those sortase residues that are absolutely conserved within the active site of S. aureus SrtA. Actinomyces naeslundii, Bacillus subtilis, Enterococcus faecalis, Streptococcus pyogenes, and Streptococcus mutants sortase display absolute conservation of leucine 97, histidine 120, tyrosine 153, leu ...
... all those sortase residues that are absolutely conserved within the active site of S. aureus SrtA. Actinomyces naeslundii, Bacillus subtilis, Enterococcus faecalis, Streptococcus pyogenes, and Streptococcus mutants sortase display absolute conservation of leucine 97, histidine 120, tyrosine 153, leu ...
Molecular genetics of the extracellular lipase of
... The structural gene (&A) coding for the extracellularlipase of Pseudomonus aeruginusa PAOl has been cloned on plasmid pSW118. Nucleotide sequence analysis revealed a gene of 936 bp. 1ipA codes for a proenzyme of 311 amino acids including a leader sequence of 26 amino acids. The mature protein was pr ...
... The structural gene (&A) coding for the extracellularlipase of Pseudomonus aeruginusa PAOl has been cloned on plasmid pSW118. Nucleotide sequence analysis revealed a gene of 936 bp. 1ipA codes for a proenzyme of 311 amino acids including a leader sequence of 26 amino acids. The mature protein was pr ...
THE INFLUENCE OF BODY MASS INDEX (BMI)
... nutritional guidelines for critically ill hospitalized patients. Improved nutritional support could decrease the risk of protein calorie malnutrition and its related complications and improve the chance of a positive outcome for critically ill patients. ...
... nutritional guidelines for critically ill hospitalized patients. Improved nutritional support could decrease the risk of protein calorie malnutrition and its related complications and improve the chance of a positive outcome for critically ill patients. ...
Histidine Biosynthetic Pathway and Genes: Structure
... FIG. 1. Gene structure of the his operon of S. typhimurium and metabolic pathway of histidine biosynthesis. Top: the leader region (L) and structural genes are drawn to scale. Arrows indicate the relative positions of P1 (primary promoter) and P2 and P3 (internal promoters). The solid box represents ...
... FIG. 1. Gene structure of the his operon of S. typhimurium and metabolic pathway of histidine biosynthesis. Top: the leader region (L) and structural genes are drawn to scale. Arrows indicate the relative positions of P1 (primary promoter) and P2 and P3 (internal promoters). The solid box represents ...
Structural Basis of Biological Nitrogen Fixation
... amino acid sequences characteristic of nucleotidebinding proteins;49,50 GXXXXGKS (the Walker motif A) which is present between residues 9 to 16, and the second Walker motif, DXXG found at residues 125-128. However, there are several reasons to believe an alternative binding mode based upon the struc ...
... amino acid sequences characteristic of nucleotidebinding proteins;49,50 GXXXXGKS (the Walker motif A) which is present between residues 9 to 16, and the second Walker motif, DXXG found at residues 125-128. However, there are several reasons to believe an alternative binding mode based upon the struc ...
A siderophore biosynthesis gene cluster from the fish
... siderophore which is distinct from that produced by P. damselae subsp. damselae. Using suppression subtractive hybridization, a subsp. piscicida-specific DNA region of 35 kb was identified in strain DI21, and 11 genes were defined: dahP, araC1, araC2, frpA, irp8, irp2, irp1, irp3, irp4, irp9 and irp ...
... siderophore which is distinct from that produced by P. damselae subsp. damselae. Using suppression subtractive hybridization, a subsp. piscicida-specific DNA region of 35 kb was identified in strain DI21, and 11 genes were defined: dahP, araC1, araC2, frpA, irp8, irp2, irp1, irp3, irp4, irp9 and irp ...
A Method To Define the Carboxyl Terminal of Proteins
... precursor m/z value for the entire LC-MS/MS run. The m/z values used in these MS/MS analyses were determined by MALDI-MS characterization, initially assuming that the charge state of the peptide ions of interest observed in the electrospray ionization measurement was 2+. For the MS/MS analyses shown ...
... precursor m/z value for the entire LC-MS/MS run. The m/z values used in these MS/MS analyses were determined by MALDI-MS characterization, initially assuming that the charge state of the peptide ions of interest observed in the electrospray ionization measurement was 2+. For the MS/MS analyses shown ...
Identification of a molecular component of the mitochondrial
... However, eukaryotic orthologues to Pat have not been identified in either the mitochondrial or nuclear genome [9]. An alternative scenario in eukaryotes could be that mitochondrial proteins are acetylated in the cytosol prior to mitochondrial import. However, as fasting and feeding result in a dynam ...
... However, eukaryotic orthologues to Pat have not been identified in either the mitochondrial or nuclear genome [9]. An alternative scenario in eukaryotes could be that mitochondrial proteins are acetylated in the cytosol prior to mitochondrial import. However, as fasting and feeding result in a dynam ...
PCNA Protein Expression during Spermatogenesis of the
... ABSTRACT—Spermatogenesis can be initiated by a single injection of human chorionic gonadotropin (hCG) into the cultivated Japanese eel, which produces only spermatogonia in the testis. To isolate the genes responsible for regulating spermatogenesis, we performed a differential mRNA display using pol ...
... ABSTRACT—Spermatogenesis can be initiated by a single injection of human chorionic gonadotropin (hCG) into the cultivated Japanese eel, which produces only spermatogonia in the testis. To isolate the genes responsible for regulating spermatogenesis, we performed a differential mRNA display using pol ...
Document
... expression analysis of yellow perch IGF-I revealed a second yellow perch transcript (IGF-Ia) that is 81 nucleotides smaller. Both IGF-Ib and IGFIa had the greatest expression in liver tissue with moderate expression in brain, spleen and kidney tissues of both sexes. These sequences are valuable mole ...
... expression analysis of yellow perch IGF-I revealed a second yellow perch transcript (IGF-Ia) that is 81 nucleotides smaller. Both IGF-Ib and IGFIa had the greatest expression in liver tissue with moderate expression in brain, spleen and kidney tissues of both sexes. These sequences are valuable mole ...
Cloning and Expression of Bovine Sodium/Glucose Cotransporters* J. Dairy Sci. 88:182–194
... this study are listed in Table 1. The 3′ and 5′ sequences of bovine solute carrier family 5 (sodium/glucose cotransporter) member 1 and 5 (bSGLT1 and bSGLT5) were obtained by rapid amplification of cDNA ends (RACE) using the SMART RACE cDNA amplification kit (Clontech, Palo Alto, CA). The 3′ and 5′ ...
... this study are listed in Table 1. The 3′ and 5′ sequences of bovine solute carrier family 5 (sodium/glucose cotransporter) member 1 and 5 (bSGLT1 and bSGLT5) were obtained by rapid amplification of cDNA ends (RACE) using the SMART RACE cDNA amplification kit (Clontech, Palo Alto, CA). The 3′ and 5′ ...
Relationship Between the Occurrence of Cysteine in Proteins and
... First, we investigated the occurrence of cysteine in known proteins of various species. In order to do this, the occurrences of the 20 coded amino acids were counted in representative samples (data files) of humans, bovines, mice, fruit flies, C. elegans, maize, rice, tomatoes, yeast, Cyanobacteria, ...
... First, we investigated the occurrence of cysteine in known proteins of various species. In order to do this, the occurrences of the 20 coded amino acids were counted in representative samples (data files) of humans, bovines, mice, fruit flies, C. elegans, maize, rice, tomatoes, yeast, Cyanobacteria, ...
Applied and Environmental Microbiology
... be the result of gene duplication. In the case of the R. tropici citrate synthase genes, the duplication seems to be ancient since the DNA sequence has clearly diverged outside the coding region, while there is a high degree of similarity in the coding regions of both genes. Besides duplication, the ...
... be the result of gene duplication. In the case of the R. tropici citrate synthase genes, the duplication seems to be ancient since the DNA sequence has clearly diverged outside the coding region, while there is a high degree of similarity in the coding regions of both genes. Besides duplication, the ...
Planta
... is dependent on the presence of the divalent cations Mg2+ or Mn2+ (Silver and Fall 1995). As in monoterpene synthases, two typical arginine residues are present (Fig. 1), which are preceded by an N-terminal transit peptide sequence, indicating that the protein is transported into the plastids (Wise ...
... is dependent on the presence of the divalent cations Mg2+ or Mn2+ (Silver and Fall 1995). As in monoterpene synthases, two typical arginine residues are present (Fig. 1), which are preceded by an N-terminal transit peptide sequence, indicating that the protein is transported into the plastids (Wise ...
Free Sample - Buy Test banks and Solution Manuals
... 43) Scientists have sequenced proteins by using specific proteases to "clip" a purified protein preparation between two specific amino acids, thus forming a number of moderately sized fragments; they have used acid hydrolysis to produce smaller fragments. Each fragment can then be sequenced by break ...
... 43) Scientists have sequenced proteins by using specific proteases to "clip" a purified protein preparation between two specific amino acids, thus forming a number of moderately sized fragments; they have used acid hydrolysis to produce smaller fragments. Each fragment can then be sequenced by break ...
GuanHongLi (275
... protein-derived peptides can have ACE-inhibiting properties and thus may be used as a novel functional food for preventing hypertension as well as for therapeutic purposes. In the present study, rice protein was hydrolyzed by protease Alcalase for 2 h and the resulted hydrolysate was determined for ...
... protein-derived peptides can have ACE-inhibiting properties and thus may be used as a novel functional food for preventing hypertension as well as for therapeutic purposes. In the present study, rice protein was hydrolyzed by protease Alcalase for 2 h and the resulted hydrolysate was determined for ...
Steven`s project - The University of Texas at Dallas
... above. However, if you intend to repack proteins quite regularly, or need to repack in excess of 200 residues, then you have no other choice. The downloaded version is also necessary if you intend to use the docking portions of the Rosetta software. The uses of the software as well as sample command ...
... above. However, if you intend to repack proteins quite regularly, or need to repack in excess of 200 residues, then you have no other choice. The downloaded version is also necessary if you intend to use the docking portions of the Rosetta software. The uses of the software as well as sample command ...