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... converting the cholesterol to a more hydrophobic form.  Cholesteryl esters are transported in secreted lipoprotein particles to other tissues that use cholesterol, or they are stored in the liver. ...
C urrent and prospective applications of metal ion–protein
C urrent and prospective applications of metal ion–protein

... 2. Immobilized metal ion affinity chromatography: utilization of differences in protein–metal ion affinity ......................................... 2.1. Principles and procedures ........................................................................................................................ ...
Darnell, JC, Warren, ST and Darnell, RB: The fragile X mental retardation protein, FMRP, recognizes G-quartets. Mental Retardation and Developmental Disabilities Research Reviews 10:49-52 (2004).
Darnell, JC, Warren, ST and Darnell, RB: The fragile X mental retardation protein, FMRP, recognizes G-quartets. Mental Retardation and Developmental Disabilities Research Reviews 10:49-52 (2004).

... involved in neuritic extension and develMRDD RESEARCH REVIEWS ...
A Simple Method for Displaying the Hydropathic Character of a Protein
A Simple Method for Displaying the Hydropathic Character of a Protein

... Ideally, the most satisfying way to determine the hydrophobic or hydrophilic inclinations of a given amino acid side-chain (i.e. its hydropathyt) would be to measure its partition coefficient between water and a non-interacting, isotropic phase and to calculate from that partition coefficient a tran ...
Allosteric Inhibition of Aminoglycoside Phosphotransferase by a
Allosteric Inhibition of Aminoglycoside Phosphotransferase by a

... APH is structurally homologous to the catalytic domain of the eukaryotic Ser/Thr/Tyr protein kinases (EPKs; for example, the mitogen-activated protein kinase p38 [Wang et al., 1997], the insulin receptor kinase [Hubbard et al., 1994], or the Abelson tyrosin kinase [ABL] [Schindler et al., 2000]). It ...
Additional file 1
Additional file 1

... Important role in various biological processes such as embryo development, root hair growth, seed coat development, defense etc. Ribosome (ath03010) ...
University of Chicago Department of Human
University of Chicago Department of Human

... copy number variation, protein expression and protein-protein interaction. In addition, the course covers issues in study design and statistical analysis of large data sets, as well as how data from different sources can be used to understand regulatory networks, i.e., systems. Statistical tools tha ...
Disorder Prediction Methods, Their Applicability to Different Protein
Disorder Prediction Methods, Their Applicability to Different Protein

... PubMed since 1990. The early 2000’s saw a dramatic increase in research on these proteins. This figure has been updated from [1] using the same search terms within PubMed; intrinsically disordered, intrinsically unstructured, natively unfolded, intrinsically unfolded and intrinsically flexible. Diso ...
A decrease in cellular energy status stimulates PERK
A decrease in cellular energy status stimulates PERK

... kinase; IRE1, inositol requiring enzyme 1; KRB, Krebs–Ringer bicarbonate buffer; tRNAi Met , methionyl initiator tRNA; mGCN2, mammalian orthologue of the yeast GCN2 (general control non-derepressible 2) protein kinase; MIN6, mouse insulinoma cell line 6; PKR, protein kinase RNA activated; PERK, PKR- ...
Support Vector Machine-based classification of protein folds using
Support Vector Machine-based classification of protein folds using

... computational prediction of 3D structures has, therefore, become a necessary alternative to experimental determination of 3D structures. Among the computational approaches, fold recognition/threading methods have taken central stage. In instances where detection of homology becomes difficult even wh ...
Internal expression of Yarrowia NDH2
Internal expression of Yarrowia NDH2

... 2000b) in the diploid strain GB1, we failed to isolate haploid spores carrying these deletion alleles, either by random spore analysis or by ascus dissection. As we also failed to introduce the deletion alleles directly into haploid yeast strains, it seemed that complex I was essential for vegetativ ...
APPLICATION OF PANCREATIC ENZYMES IN HYDROLYSIS OF
APPLICATION OF PANCREATIC ENZYMES IN HYDROLYSIS OF

... The  substrate, containing ovoalbumin (45  kDa) and ovotransferrin (78  kDa), was obtained as a  by-product in the proteinaceous agent of an antimicrobial activity isolation protocol [Graszkiewicz et al., 2007; Sokołowska et al., 2007]. This inexpensive and simple procedure allows obtaining a  mixtu ...
Small decrease of plastid transketolase activity in
Small decrease of plastid transketolase activity in

... Using a heterologous sequence from resurrection plant (Bernacchia et al., 1995), we isolated a full-length tobacco cDNA (TK-23; EMBL accession number A52295) with homology to TK from resurrection plant (69%), potato (89%), and yeast (44%). The N terminus contained a 77–amino acid plastid transit pep ...
Beneficial effects of L-arginine on reducing obesity
Beneficial effects of L-arginine on reducing obesity

... biogenesis (Lehman et al. 2000; Puigserver et al. 1998; Wu et al. 1999) and its expression is influenced by NO (Fu et al. 2005). In turn, PGC-1a stimulates expression of key genes involved in mitochondrial biogenesis, including nuclear respiratory factors 1 and 2, as well as PPARa (Table 1). Studies ...
A1071 GM Canola MON88302 AppR SD1
A1071 GM Canola MON88302 AppR SD1

... range compiled from results taken for seven non-GM commercial lines grown under the same conditions and c) levels recorded in the literature. A total of 69 analytes were measured of which 18 fatty acids and sodium had 50% of their values below the assay limit of quantitation and were therefore not u ...
View/Open - VUW research archive - Victoria University of Wellington
View/Open - VUW research archive - Victoria University of Wellington

... Conditions for extraction and two-dimensional electrophoresis of proteins were established. One hundred and seventy nine proteins were identified by MALDI mass spectrometry of tryptic digests of protein spots excised from Coomassie stained gels. All of the enzymes for conversion of glucose to ethano ...
Tetrahydrobiopterin and its functions
Tetrahydrobiopterin and its functions

... Tetrahydrobiopterin (BH4) is essential for diverse processes and is ubiquitously present in all tissues of higher organisms. It is well established as essential cofactor for various enzyme activities to activate dioxygen, and for less defined functions at the cellular level. The latter function of B ...
ppt - Chair of Computational Biology
ppt - Chair of Computational Biology

... For a random network P(k) peaks strongly at k = and decays exponentially for large k (i.e., P(k)  e-k for k >> and k << ). c, In the scale-free network most nodes have only a few links, but a few nodes, called hubs (dark), have a very large ...
Glucose transporters (GLUT and SGLT)
Glucose transporters (GLUT and SGLT)

... dietary glucose with fructose. The magnitude of this disorder, by the mutation of a single gene, would indicate that alternative lumenal glucose transport does not occur to any significant extent. The relevance of SGLT3 intestinal expression needs to be addressed and suggests that care should be tak ...
Word - The Open University
Word - The Open University

... To make things quicker to draw when we are dealing with larger molecules, the atoms are not usually represented as coloured balls but by the chemical symbol for the element, and the bonds between the atoms are drawn as lines. Chemical analysis of the human body shows that 13 major elements, with sma ...
Towards the storage metabolome: profiling the barley vacuole
Towards the storage metabolome: profiling the barley vacuole

... Technological developments in the last decade have rendered metabolomics, the comprehensive study of small molecule metabolites, an important functional genomic tool (Fiehn, 2002; Sumner et al., 2003; Weckwerth, 2003; Farre et al., 2006; Matsuda et al., 2009; Saito and Matsuda, 2010). It is currentl ...
Table xx - Hindawi
Table xx - Hindawi

... induced) than in line B. These subpathways show equal expression between the lines after 24 h infection with Salmonella. It can be concluded that this equal expression levels is the result of a higher up regulation in line A than in line B. Leukocyte transendothelial migration: Activation of leukocy ...
Role of Dietary Protein in Post-Exercise Muscle Reconditioning
Role of Dietary Protein in Post-Exercise Muscle Reconditioning

... properties, which can be attributed to differences in digestion and absorption kinetics as well as amino acid composition [23, 24]. Whey protein is a soluble protein that is rapidly digested and absorbed following ingestion. In contrast, intact casein tends to clot in the stomach after ingestion, th ...
Low Oxygen Response Mechanisms in Green Organisms
Low Oxygen Response Mechanisms in Green Organisms

... Many studies suggest that submergence tolerance in several plant species may be associated with the ability to mobilize carbohydrate reserves, and also that the most important enzymes, which are involved in the degradation of carbohydrates, seem to play a very important role. 2. Low Oxygen Sensing a ...
Intersubunit contacts are often facilitated by specificity
Intersubunit contacts are often facilitated by specificity

... The LacI family of bacterial transcription factors ...
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Expression vector

An expression vector, otherwise known as an expression construct, is usually a plasmid or virus designed for protein expression in cells. The vector is used to introduce a specific gene into a target cell, and can commandeer the cell's mechanism for protein synthesis to produce the protein encoded by the gene. Expression vectors are the basic tools in biotechnology for the production of proteins.The plasmid is engineered to contain regulatory sequences that act as enhancer and promoter regions and lead to efficient transcription of the gene carried on the expression vector. The goal of a well-designed expression vector is the production of protein, and this may be achieve by the production of significant amount of stable messenger RNA, which can then be translated into protein. The protein may be expressed constitutively, or induced when necessary using an inducer. Escherichia coli is commonly used as the host for protein expression, other cell types however may also be used. An example of the use of expression vector is the production of insulin which is used for medical treatments of diabetes.
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