Human Skeletal Muscle Expresses a Glycogen
... of three BAC genomic clones established that there was only one GL gene within each clone, indicating that GL is encoded by a single gene in the human genome. Expression of GL mRNA and protein in several tissues, including heart and skeletal muscle. Analysis of the tissue distribution of human GL mR ...
... of three BAC genomic clones established that there was only one GL gene within each clone, indicating that GL is encoded by a single gene in the human genome. Expression of GL mRNA and protein in several tissues, including heart and skeletal muscle. Analysis of the tissue distribution of human GL mR ...
Planta - University of Regina
... instructions. Following RNase H treatment, the resulting singlestrand cDNA mixture was used as a template for the polymerase chain reaction (PCR). For PCR ampli®cation of the core region of the cDNA encoding CHS-like enzymes, two sets of degenerate oligonucleotide primers (see Fig. 2) were synthesiz ...
... instructions. Following RNase H treatment, the resulting singlestrand cDNA mixture was used as a template for the polymerase chain reaction (PCR). For PCR ampli®cation of the core region of the cDNA encoding CHS-like enzymes, two sets of degenerate oligonucleotide primers (see Fig. 2) were synthesiz ...
Sensing the fat: Fatty acid metabolism in the
... CART [27]. Also, in this study cerulenin was highly effective at reducing body weight in agouti (Ay ) mice, in which obesity is caused by blockade of the melanocortin receptor, suggesting melanocortin-independence [27]. Conversely, a more recent paper suggests that the actions of cerulenin, as in th ...
... CART [27]. Also, in this study cerulenin was highly effective at reducing body weight in agouti (Ay ) mice, in which obesity is caused by blockade of the melanocortin receptor, suggesting melanocortin-independence [27]. Conversely, a more recent paper suggests that the actions of cerulenin, as in th ...
Chromosomal rearrangements and protein globularity changes in
... PPE53 and PPE24) were found in at least four of the strains. The variants in eight of these genes led to amino acid changes but only two altered genes have known functions: PE_PGRS19, a putative outer membrane protein (Song et al., 2008) and embR which is involved in transcription, the biosynthesis ...
... PPE53 and PPE24) were found in at least four of the strains. The variants in eight of these genes led to amino acid changes but only two altered genes have known functions: PE_PGRS19, a putative outer membrane protein (Song et al., 2008) and embR which is involved in transcription, the biosynthesis ...
Protein Applications in Sports Nutrition—Part I
... acids. Typically, if a protein source comes from an animal, such as beef, pork, fish, egg, milk (or dairy products), and poultry, such as chicken or turkey, it is considered a complete source of protein. Proteins derived from plants are typically incomplete meaning they completely lack one or more o ...
... acids. Typically, if a protein source comes from an animal, such as beef, pork, fish, egg, milk (or dairy products), and poultry, such as chicken or turkey, it is considered a complete source of protein. Proteins derived from plants are typically incomplete meaning they completely lack one or more o ...
Source–Sink Relationships
... invertase, SS and SPS are early events in the transition from net sucrose import to sucrose export. Indeed, the ratio of SS to SPS can often be used as an indicator of the sink to source transition. Unlike animals, which use glucose as a transport sugar, plants transport sucrose. We can only specula ...
... invertase, SS and SPS are early events in the transition from net sucrose import to sucrose export. Indeed, the ratio of SS to SPS can often be used as an indicator of the sink to source transition. Unlike animals, which use glucose as a transport sugar, plants transport sucrose. We can only specula ...
Significance of Intestinal Digestion of Dietary Protein
... compared these values with true intestinal digestibility of proteins according to tabular values for 29 feeds. Tabular values were found to be generally higher than those estimated using the MBT (89.8 vs 84.4%). With both evaluation methods, feeds of plant origin seemed more digestible than those of ...
... compared these values with true intestinal digestibility of proteins according to tabular values for 29 feeds. Tabular values were found to be generally higher than those estimated using the MBT (89.8 vs 84.4%). With both evaluation methods, feeds of plant origin seemed more digestible than those of ...
Three multidomain esterases from the cellulolytic
... (McDermid et al., 1990 ; Hespell & O’Bryan-Shah, 1988). There is little evidence so far for the involvement of esterase activities in cell wall degradation by Ruminococcus spp. (Akin et al., 1993), which represent one of the most numerous groups of cellulolytic bacteria in the rumen. Although it has ...
... (McDermid et al., 1990 ; Hespell & O’Bryan-Shah, 1988). There is little evidence so far for the involvement of esterase activities in cell wall degradation by Ruminococcus spp. (Akin et al., 1993), which represent one of the most numerous groups of cellulolytic bacteria in the rumen. Although it has ...
Accurate Prediction of Contact Numbers for Multi
... where n is the length of the protein chain in the case of computing the monomeric contact number or the total number of residues in the protein for computing the oligomeric contact number. All nonprotein molecules were removed before computing the contact numbers. Nonprotein molecules such as coenzy ...
... where n is the length of the protein chain in the case of computing the monomeric contact number or the total number of residues in the protein for computing the oligomeric contact number. All nonprotein molecules were removed before computing the contact numbers. Nonprotein molecules such as coenzy ...
endoplasmic reticulum stress and lipid metabolism
... and is studded with ribosomes, thus the cisternal sheets are the location of protein synthesis and folding. The tubular ER is enriched in tissues specializing in the biosynthesis of lipids ...
... and is studded with ribosomes, thus the cisternal sheets are the location of protein synthesis and folding. The tubular ER is enriched in tissues specializing in the biosynthesis of lipids ...
A novel multifunctional O-methyltransferase implicated in a dual
... (Invitrogen) to ensure the expression of AEOMT using AEOMT-specific start and stop codons. pPICZ A vector containing AEOMT cDNA was transferred into E. coli JM109, and transformed cells were selected on 25 mgyml Zeocin as an antibiotic selection marker (Invitrogen). Forty micrograms of plasmid DNA w ...
... (Invitrogen) to ensure the expression of AEOMT using AEOMT-specific start and stop codons. pPICZ A vector containing AEOMT cDNA was transferred into E. coli JM109, and transformed cells were selected on 25 mgyml Zeocin as an antibiotic selection marker (Invitrogen). Forty micrograms of plasmid DNA w ...
Biological Networks Underlying Abiotic Stress Tolerance in
... structure and metabolism [5]. They are products of genes, but they are much closer to the resulting phenotype since they act as direct effectors of the phenotype, i.e., they constitute plant cell structure and actively participate on metabolism of all cellular components. The total of all proteins i ...
... structure and metabolism [5]. They are products of genes, but they are much closer to the resulting phenotype since they act as direct effectors of the phenotype, i.e., they constitute plant cell structure and actively participate on metabolism of all cellular components. The total of all proteins i ...
Directions: Choose the BEST answer from among those given.
... e) choose this answer if none of these is the best choice 22)A common structural motif of proteins in which a linear sequence of amino acids folds into a righthanded elongated structure that twists in regular corkscrew fashion around a central axis, and is stabilized by internal hydrogen bonding bet ...
... e) choose this answer if none of these is the best choice 22)A common structural motif of proteins in which a linear sequence of amino acids folds into a righthanded elongated structure that twists in regular corkscrew fashion around a central axis, and is stabilized by internal hydrogen bonding bet ...
Full-Text PDF
... Table 1. As development progresses, both fruit weight and diameter (data not shown) exhibit a double sigmoid curve with a short latency period (stage II), which is typical growth for this fruit during a season with predominantly low temperatures [26]. Although a series of studies have examined the c ...
... Table 1. As development progresses, both fruit weight and diameter (data not shown) exhibit a double sigmoid curve with a short latency period (stage II), which is typical growth for this fruit during a season with predominantly low temperatures [26]. Although a series of studies have examined the c ...
characterization of proteins from the cytoskeleton of giardia lamblia
... Two of the bands are a- and /J-tubulin. After staining with PAGE Blue 83, densitometry showed that the tubulins account for 20-26 % of the stained material, which is the same result as reported earlier for G. duodenalis cytoskeletons. The third prominent band is a 30 X 103 Mr protein, which often mi ...
... Two of the bands are a- and /J-tubulin. After staining with PAGE Blue 83, densitometry showed that the tubulins account for 20-26 % of the stained material, which is the same result as reported earlier for G. duodenalis cytoskeletons. The third prominent band is a 30 X 103 Mr protein, which often mi ...
SIZE EXCLUSION AND HI CHROMATOGRAPHY
... • Study of biological processes (example: synthesis of proteins) • Localization and regulation of gene expression • Cell movement • Cell fate during development • Formation of different organs • Screenable marker to identify transgenic organisms ...
... • Study of biological processes (example: synthesis of proteins) • Localization and regulation of gene expression • Cell movement • Cell fate during development • Formation of different organs • Screenable marker to identify transgenic organisms ...
AlgPred: prediction of allergenic proteins and mapping of
... WHO 2001 recommendations and recognized the uncertainties associated with different tests. They suggest weight of evidence approach rather than a specific decision tree approach (suggested in FAO/WHO 2001). They recommended various tests for examining allergenic behavior of proteins that includes so ...
... WHO 2001 recommendations and recognized the uncertainties associated with different tests. They suggest weight of evidence approach rather than a specific decision tree approach (suggested in FAO/WHO 2001). They recommended various tests for examining allergenic behavior of proteins that includes so ...
Relationship between codon biased genes, microarray expression
... Comparison of CAI and microarray fluorescence values As 85 % of the genes of the R6 and TIGR4 strains have a similarity above 90 %, and a good correlation (r2=0?99) of CAI values among their homologous genes was observed (data not shown), Cy3- (two replicates) and Cy5- (one replicate) labelled cDNA ...
... Comparison of CAI and microarray fluorescence values As 85 % of the genes of the R6 and TIGR4 strains have a similarity above 90 %, and a good correlation (r2=0?99) of CAI values among their homologous genes was observed (data not shown), Cy3- (two replicates) and Cy5- (one replicate) labelled cDNA ...
The Phosphoproteome of a Chlamydomonas reinhardtii Eyespot
... proteomic approach, we identified 202 proteins from a fraction enriched in eyespot apparatuses of Chlamydomonas reinhardtii. Among these proteins, five protein kinases and two protein phosphatases were present, indicating that reversible protein phosphorylation occurs in the eyespot. About 20 major ...
... proteomic approach, we identified 202 proteins from a fraction enriched in eyespot apparatuses of Chlamydomonas reinhardtii. Among these proteins, five protein kinases and two protein phosphatases were present, indicating that reversible protein phosphorylation occurs in the eyespot. About 20 major ...
Oxytocin Hormone synthesis and regulation in the Body
... Oxytocin Hormone synthesis and regulation in the Body Oxytocin synthesis and function: The hypothalamus plays an important role in the body by synthesizing and regulating hormones that modulate intercellular communication and body’s function. Hormones produced by the hypothalamus are organized ...
... Oxytocin Hormone synthesis and regulation in the Body Oxytocin synthesis and function: The hypothalamus plays an important role in the body by synthesizing and regulating hormones that modulate intercellular communication and body’s function. Hormones produced by the hypothalamus are organized ...
DOC-file of additional text
... IV. Receiver Operating Characteristic Curve (ROC) method Two values were assigned to each E. coli metabolic gene: a binary variable representing presence or absence of the gene in a given Buchnera genome, and the number of occurrences of the gene in 500 simulated reduced genomes. For each cut-off ( ...
... IV. Receiver Operating Characteristic Curve (ROC) method Two values were assigned to each E. coli metabolic gene: a binary variable representing presence or absence of the gene in a given Buchnera genome, and the number of occurrences of the gene in 500 simulated reduced genomes. For each cut-off ( ...
Evidence for the presence of a glucosensor in hypothalamus
... distinct grouping called the Brockmann bodies (BB) exists near to the gall bladder. The BB are formed by endocrine cells, mainly -cells with fewer - and -cells than found in the mammalian Islets of Langerhans (44). Little information is reported in fish regarding the presence of glucosensors in panc ...
... distinct grouping called the Brockmann bodies (BB) exists near to the gall bladder. The BB are formed by endocrine cells, mainly -cells with fewer - and -cells than found in the mammalian Islets of Langerhans (44). Little information is reported in fish regarding the presence of glucosensors in panc ...
Avian-to-human transmission of the PB1 gene of influenza A viruses in the 1957 and 1968 pandemics
... were compared with those of influenza B virus (15), approximately 60% homology was detected. The nucleotide sequences were analyzed by the maximum-parsimony method, and an evolutionary tree was constructed (Fig. 1). The diagram indicates four distinct lineages for the PB1 gene, the first comprising ...
... were compared with those of influenza B virus (15), approximately 60% homology was detected. The nucleotide sequences were analyzed by the maximum-parsimony method, and an evolutionary tree was constructed (Fig. 1). The diagram indicates four distinct lineages for the PB1 gene, the first comprising ...
PCR Cloning Considerations
... 1:5. While these ratios may not be ideal for all cloning events, they are useful for most cloning needs. For example, if the vector is 3 kb and the insert is 1 kb, one-third the amount of insert needs to be added to attain a 1:1 molar ratio. When performing TOPO® -TA or Directional TOPO® Cloning, op ...
... 1:5. While these ratios may not be ideal for all cloning events, they are useful for most cloning needs. For example, if the vector is 3 kb and the insert is 1 kb, one-third the amount of insert needs to be added to attain a 1:1 molar ratio. When performing TOPO® -TA or Directional TOPO® Cloning, op ...
Poon, Andy: Predicting Phosphorylation: A critique of the NetPhos program and potential alternatives
... The purpose of using quantitative pattern matching was to possibly identify known motifs common to phosphorylated proteins. Perhaps documented motifs existed, initially attributed to other protein features, which may be indicative of phosphorylation as well. (For instance, perhaps these databases r ...
... The purpose of using quantitative pattern matching was to possibly identify known motifs common to phosphorylated proteins. Perhaps documented motifs existed, initially attributed to other protein features, which may be indicative of phosphorylation as well. (For instance, perhaps these databases r ...