Types and effects of protein variations. Vihinen
... interactions and other characteristics in several ways. List of the effects would be really long and include e.g. interactions, stability, electrostatic effects, protein packing, local and global structural changes and so on. Many deleterious variants are straightforward to explain, such as large de ...
... interactions and other characteristics in several ways. List of the effects would be really long and include e.g. interactions, stability, electrostatic effects, protein packing, local and global structural changes and so on. Many deleterious variants are straightforward to explain, such as large de ...
Production of a Brassica napus Low-Molecular
... protein lacking two segments (20 and 17 amino acids in length, respectively) normally present within its hydrophobic domain, thereby potentially reducing its targeting efficiency to oil bodies. Consistent with this premise, microscopy-based localization studies with OleoH3PACBP fused to GFP (GFP-Oleo ...
... protein lacking two segments (20 and 17 amino acids in length, respectively) normally present within its hydrophobic domain, thereby potentially reducing its targeting efficiency to oil bodies. Consistent with this premise, microscopy-based localization studies with OleoH3PACBP fused to GFP (GFP-Oleo ...
The aconitase of Escherichia cok purification of the
... University of Shefield, PO Box 594,Firth Court, Western Bank, Shefield SIO 2 U H , UK (Received 24 April 1991; revised I0 July 1991;accepted 24 July 1991) The aconitase of Escherichia cofi was purified to homogeneity, albeit in low yield (0.6%). It was shown to be a monomeric protein of Mr95000 or 9 ...
... University of Shefield, PO Box 594,Firth Court, Western Bank, Shefield SIO 2 U H , UK (Received 24 April 1991; revised I0 July 1991;accepted 24 July 1991) The aconitase of Escherichia cofi was purified to homogeneity, albeit in low yield (0.6%). It was shown to be a monomeric protein of Mr95000 or 9 ...
Free amino acids are important for the retention of protein and non
... It is difficult to compare the experiments of Cole and Gillett with those reported here; we looked at the presence of meal constituents in the midgut and faeces (protein and FITC-dextran), while they looked at elimination of the dark brown haem-containing faeces. These two processes are likely contr ...
... It is difficult to compare the experiments of Cole and Gillett with those reported here; we looked at the presence of meal constituents in the midgut and faeces (protein and FITC-dextran), while they looked at elimination of the dark brown haem-containing faeces. These two processes are likely contr ...
Journal of Bacteriology
... amyloliquefaciens FZB 42 were identified in extracts of lyophilized culture filtrates and XAD7 extracts by MALDI-TOF MS. Mass spectra were recorded with a Bruker Daltonik Reflex MALDI-TOF instrument containing a 337-nm nitrogen laser for desorption and ionization. One hundred to 200 single scans wer ...
... amyloliquefaciens FZB 42 were identified in extracts of lyophilized culture filtrates and XAD7 extracts by MALDI-TOF MS. Mass spectra were recorded with a Bruker Daltonik Reflex MALDI-TOF instrument containing a 337-nm nitrogen laser for desorption and ionization. One hundred to 200 single scans wer ...
Current Opinion in Plant Biology
... AHL produce reduced or even non-detectable amounts of AFMs. It was shown very recently that a mutation in the lexA gene of Pseudomonas chlororaphis PCL1391 resulted in a ten-fold increase in phenazine-1-carboxamide production, which can be explained by the fact that the lexA mutant produces elevated ...
... AHL produce reduced or even non-detectable amounts of AFMs. It was shown very recently that a mutation in the lexA gene of Pseudomonas chlororaphis PCL1391 resulted in a ten-fold increase in phenazine-1-carboxamide production, which can be explained by the fact that the lexA mutant produces elevated ...
An Engineered Aryl Azide Ligase for Site-Specific
... PPI detection by photo-cross-linking has considerable advantages over other more commonly used PPI detection methods, such as the yeast two-hybrid technique,[3] protein complementation assays (based on green fluorescent protein,[4] split b-lactamase,[5] and split luciferase),[6, 7] and fluorescence ...
... PPI detection by photo-cross-linking has considerable advantages over other more commonly used PPI detection methods, such as the yeast two-hybrid technique,[3] protein complementation assays (based on green fluorescent protein,[4] split b-lactamase,[5] and split luciferase),[6, 7] and fluorescence ...
Table S10. List of the Databases and Software used in the H
... http://crick.genes.nig.ac.jp/homology/clustalw.shtml ...
... http://crick.genes.nig.ac.jp/homology/clustalw.shtml ...
Inflammation and ER Stress Regulate Branched
... the manufacturer’s protocol. RNA was treated with deoxyribonuclease I, and cDNA was synthesized using an iScript cDNA synthesis kit (Bio-Rad Laboratories). Amplification was monitored with iQ SYBR Green Supermix and the MyiQ detection system (BioRad Laboratories). Data were analyzed using the –⌬⌬cyc ...
... the manufacturer’s protocol. RNA was treated with deoxyribonuclease I, and cDNA was synthesized using an iScript cDNA synthesis kit (Bio-Rad Laboratories). Amplification was monitored with iQ SYBR Green Supermix and the MyiQ detection system (BioRad Laboratories). Data were analyzed using the –⌬⌬cyc ...
Histone Deacetylase 4 Antibody
... members, HDAC-1, 2, 3, and 8, each of which contains a deacetylase domain exhibiting from 45 to 93% identity in amino acid sequence. Class II of the HDAC family comprises HDAC-4, 5, 6, and 7, the molecular weights of which are all about twofold larger than those of the class I members, and the deace ...
... members, HDAC-1, 2, 3, and 8, each of which contains a deacetylase domain exhibiting from 45 to 93% identity in amino acid sequence. Class II of the HDAC family comprises HDAC-4, 5, 6, and 7, the molecular weights of which are all about twofold larger than those of the class I members, and the deace ...
Low Protein Diet - Hamilton Health Sciences
... These specialized formulas are "custom made" for your child's metabolic disorder and provide enough protein to keep your child healthy without causing harm. If it is needed, your metabolic health care team will prescribe formula for your child. ...
... These specialized formulas are "custom made" for your child's metabolic disorder and provide enough protein to keep your child healthy without causing harm. If it is needed, your metabolic health care team will prescribe formula for your child. ...
Machine learning methods for Protein Secondary Structure Prediction
... van der Waals interactions Bond angle/length constraints Long distance, e.g. ...
... van der Waals interactions Bond angle/length constraints Long distance, e.g. ...
Regulation of transcript encoding the 43K
... present at nonsynaptic regions of denervated and developing muscle (Froehner et al. 1981; Burden, 1985; Peng & Froehner, 1985). Thus, it is not clear whether the 43K protein is associated with both synaptic and nonsynaptic AChRs or whether the 43K protein is associated with only clustered AChRs. Mor ...
... present at nonsynaptic regions of denervated and developing muscle (Froehner et al. 1981; Burden, 1985; Peng & Froehner, 1985). Thus, it is not clear whether the 43K protein is associated with both synaptic and nonsynaptic AChRs or whether the 43K protein is associated with only clustered AChRs. Mor ...
Manipulating redox and ATP balancing for improved production of
... E. coli, this step is catalyzed by PEP-carboxylase (ppc), while in Actinobacillus succinogenes, this step is catalyzed by the ATP-generating PEP-carboxykinase (Kim et al., 2004; Laivenieks et al., 1997; VanderWerf et al., 1997). Prior studies have shown that PEP-carboxylase overexpression can signifi ...
... E. coli, this step is catalyzed by PEP-carboxylase (ppc), while in Actinobacillus succinogenes, this step is catalyzed by the ATP-generating PEP-carboxykinase (Kim et al., 2004; Laivenieks et al., 1997; VanderWerf et al., 1997). Prior studies have shown that PEP-carboxylase overexpression can signifi ...
Channel-mediated lactic acid transport: a novel function for
... frames) from genomic DNA isolated from the different bacterial strains. The PCR products were directionally subcloned using a uracil excision-based improved high-throughput USER cloning technique [25] into the USER-compatible Xenopus expression vectors pNB1u and pNB1YFPu (N-terminal fusion of YFP wi ...
... frames) from genomic DNA isolated from the different bacterial strains. The PCR products were directionally subcloned using a uracil excision-based improved high-throughput USER cloning technique [25] into the USER-compatible Xenopus expression vectors pNB1u and pNB1YFPu (N-terminal fusion of YFP wi ...
Chapter 2 - University of the Free State
... Use non-linear IPG stips, since most of the proteome has pI values between 4 and 7 ...
... Use non-linear IPG stips, since most of the proteome has pI values between 4 and 7 ...
Patent Protection for the Protein Products of Recombinant DNA
... United States Code. 1 6 The utility requirement ensures that the invention is useful for some purpose. 17 To be novel, the invention must be new, so that a patent is not granted for something that already belongs to the public. 1 8 The non-obviousness requirement extends the novelty requirement and ...
... United States Code. 1 6 The utility requirement ensures that the invention is useful for some purpose. 17 To be novel, the invention must be new, so that a patent is not granted for something that already belongs to the public. 1 8 The non-obviousness requirement extends the novelty requirement and ...
Citační ohlasy podle WoS – F. Cvrčková (k 31.1.2005)
... 19. Watanabe, CMH; Supekova, L; Schultz, PG. 2002. Transcriptional effects of the potent enediyne anti-cancer agent calicheamicin gamma. CHEMISTRY & BIOLOGY 9 (2): 245-251. 20. Calzada, A; Bueno, A. 2002. Genes involved in the initiation of DNA replication in yeast. INTERNATIONAL REVIEW OF CYTOLOGY ...
... 19. Watanabe, CMH; Supekova, L; Schultz, PG. 2002. Transcriptional effects of the potent enediyne anti-cancer agent calicheamicin gamma. CHEMISTRY & BIOLOGY 9 (2): 245-251. 20. Calzada, A; Bueno, A. 2002. Genes involved in the initiation of DNA replication in yeast. INTERNATIONAL REVIEW OF CYTOLOGY ...
ppt - pedagogix
... In a database scan, each word of the query sequence is compared to each word of the database. For a query sequence of size m and a database of size n, the search space (number of word pair comparisons) is thus N=nm. FastA and BLAST estimate, for each score, the number of matches that would be expect ...
... In a database scan, each word of the query sequence is compared to each word of the database. For a query sequence of size m and a database of size n, the search space (number of word pair comparisons) is thus N=nm. FastA and BLAST estimate, for each score, the number of matches that would be expect ...
... Mycobacterium tuberculosis can persist in the human host, in a latent state, that represents an obstacle for the control of this disease. It has been suggested that, between other factors, M. tuberculosis persistence can be caused by oxygen limitation and low doses of nitric oxide. It has been obser ...
- Wiley Online Library
... (Collins et al., 2005). Plant pathogenic bacteria also display xylanolytic activities, which may help them to breach the cell wall obstacle and to release nutrients during the colonization of plants. Bioconversion of xylans has been intensively studied in the past decade because of its potential app ...
... (Collins et al., 2005). Plant pathogenic bacteria also display xylanolytic activities, which may help them to breach the cell wall obstacle and to release nutrients during the colonization of plants. Bioconversion of xylans has been intensively studied in the past decade because of its potential app ...
Structural and functional features of Drosophila chorion proteins s36
... and more than 14 minor chorion proteins can be resolved by two-dimensional gel electrophoresis; subsets of these proteins are expressed in a temporally regulated mode during the 5 h of choriogenesis 5 7. The sequences of all major chorion proteins have been determined: the lower molecular weight s15 ...
... and more than 14 minor chorion proteins can be resolved by two-dimensional gel electrophoresis; subsets of these proteins are expressed in a temporally regulated mode during the 5 h of choriogenesis 5 7. The sequences of all major chorion proteins have been determined: the lower molecular weight s15 ...
Understanding Tools and Techniques in Protein Structure Prediction
... bioinformatics, it has been made possible to understand the relationship between amino acid sequence and three-dimensional structure in proteins. The central challenge of bioinformatics is the rationalization of the mass of sequence information not only to derive efficient means of storage and retri ...
... bioinformatics, it has been made possible to understand the relationship between amino acid sequence and three-dimensional structure in proteins. The central challenge of bioinformatics is the rationalization of the mass of sequence information not only to derive efficient means of storage and retri ...