Enzyme Redundancy and the Importance of 2
... Although many biochemical studies have improved our understanding of plant N metabolism, the exact enzymes involved and the factors controlling this process are still often lacking. Many enzymes involved in ammonium assimilation are found as multiple enzyme/isoenzyme forms that are often located in ...
... Although many biochemical studies have improved our understanding of plant N metabolism, the exact enzymes involved and the factors controlling this process are still often lacking. Many enzymes involved in ammonium assimilation are found as multiple enzyme/isoenzyme forms that are often located in ...
Comparative proteomic network signatures in seminal plasma of
... Background: Reactive oxygen species (ROS) plays a major role in the pathology of male infertility. It is an independent biomarker of sperm function. Seminal plasma is a natural reservoir of antioxidants responsible for the nourishment, protection, capacitation, and motility of sperm within the fema ...
... Background: Reactive oxygen species (ROS) plays a major role in the pathology of male infertility. It is an independent biomarker of sperm function. Seminal plasma is a natural reservoir of antioxidants responsible for the nourishment, protection, capacitation, and motility of sperm within the fema ...
Conserved Tryptophan Residues within Putative Transmembrane
... The Role of Trp258 and Trp259 on OATP1B1 Transport Activity. To investigate whether the structural characteristics of Trp258 and Trp259 are essential for transport function of OATP1B1, we substituted these two tryptophan residues with phenylalanine, another amino acid that contains the aromatic ring ...
... The Role of Trp258 and Trp259 on OATP1B1 Transport Activity. To investigate whether the structural characteristics of Trp258 and Trp259 are essential for transport function of OATP1B1, we substituted these two tryptophan residues with phenylalanine, another amino acid that contains the aromatic ring ...
Dirty Business - American Chemical Society
... So we can think about many amino acids linking together via peptide bonds to form longer chains. Chains with molecular masses of less than 10,000 Da (Daltons) are not considered proteins but are usually referred to as oligopeptide chains. Longer protein chains may have thousands of amino acids, as n ...
... So we can think about many amino acids linking together via peptide bonds to form longer chains. Chains with molecular masses of less than 10,000 Da (Daltons) are not considered proteins but are usually referred to as oligopeptide chains. Longer protein chains may have thousands of amino acids, as n ...
Metabolism of sucrose and its five isomers by
... Cells of E. coli PEP43(pCB4.11) (" 25 g wet weight) were resuspended with 40 ml TNM buffer, and the organisms were disrupted by 2i1n5 min sonication with a Branson model 350 instrument. The preparation was clarified by ultracentrifugation (180 000 g for 2 h at 5 mC), and the supernatant fluid was di ...
... Cells of E. coli PEP43(pCB4.11) (" 25 g wet weight) were resuspended with 40 ml TNM buffer, and the organisms were disrupted by 2i1n5 min sonication with a Branson model 350 instrument. The preparation was clarified by ultracentrifugation (180 000 g for 2 h at 5 mC), and the supernatant fluid was di ...
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... direct interaction of metabolites with nuclear gene expression (NGE) during re trograde signaling (Section 3.2). Regulation strategies are also most likely unidirectional as transcriptional regulation of metabolic changes will lead to the metabolic induction of transcriptional signal transduction pa ...
... direct interaction of metabolites with nuclear gene expression (NGE) during re trograde signaling (Section 3.2). Regulation strategies are also most likely unidirectional as transcriptional regulation of metabolic changes will lead to the metabolic induction of transcriptional signal transduction pa ...
Homology modeling workshop
... • Restraints and CHARMM energy terms are then combined into an objective function, which is optimized in 3D space. ...
... • Restraints and CHARMM energy terms are then combined into an objective function, which is optimized in 3D space. ...
(Plant and Fungal Physiology, UMR 2847 CNRS
... healthy plant tissues extracts were detected by anti fungal antisera. As well, antiplant antisera ...
... healthy plant tissues extracts were detected by anti fungal antisera. As well, antiplant antisera ...
Effect of Six Decades of Selective Breeding on
... To evaluate the extent of the genetic change and its effects on the seed protein composition of soybean cultivars released during the past 60 years, representative ancestral cultivars and those derived from selective breeding were grown in a side-by-side comparison. Total seed protein content, deter ...
... To evaluate the extent of the genetic change and its effects on the seed protein composition of soybean cultivars released during the past 60 years, representative ancestral cultivars and those derived from selective breeding were grown in a side-by-side comparison. Total seed protein content, deter ...
Comparative analysis of proteins with a mucus
... The putative MUB domain of the protein lp_1229 of Lb. plantarum WCFS1 consists of approximately 100 aa, while the MUB domain of protein Mub of Lb. reuteri is almost 200 residues in length (Kleerebezem et al., 2003; Pretzer et al., 2005; Roos & Jonsson, 2002). This difference in size implies a discre ...
... The putative MUB domain of the protein lp_1229 of Lb. plantarum WCFS1 consists of approximately 100 aa, while the MUB domain of protein Mub of Lb. reuteri is almost 200 residues in length (Kleerebezem et al., 2003; Pretzer et al., 2005; Roos & Jonsson, 2002). This difference in size implies a discre ...
Comparison of environmental and isolate Sulfobacillus genomes
... accelerate sulfide mineral dissolution in acid mine drainage environments (AMD), acid-rock drainage environments (ARD), as well as in industrial bioleaching operations. Despite their frequent identification in these environments, their role in biogeochemical cycling is poorly understood. Results: He ...
... accelerate sulfide mineral dissolution in acid mine drainage environments (AMD), acid-rock drainage environments (ARD), as well as in industrial bioleaching operations. Despite their frequent identification in these environments, their role in biogeochemical cycling is poorly understood. Results: He ...
Triphosphatase Related to the Protein Tyrosine Phosphatases
... capping enzyme. CEL-1 has a C-terminal domain containing motifs found in yeast and vaccinia virus capping enzyme guanylyltransferases. The N-terminal domain of CEL-1 has RNA triphosphatase activity. Surprisingly, this domain does not resemble the vaccinia virus capping enzyme but does have significa ...
... capping enzyme. CEL-1 has a C-terminal domain containing motifs found in yeast and vaccinia virus capping enzyme guanylyltransferases. The N-terminal domain of CEL-1 has RNA triphosphatase activity. Surprisingly, this domain does not resemble the vaccinia virus capping enzyme but does have significa ...
Salinity Response in Chloroplasts: Insights from Gene
... The overexpression of Cu/Zn SOD in chloroplasts of Arabidopsis [16], tobacco [17,18], Chinese cabbage (Brassica campestris L. ssp. pekinensis cv. Tropical Pride) [19], and cotton [20] can enhance salinity tolerance through reducing ROS (Table 1). Similarly, tobacco plants with an overexpression of A ...
... The overexpression of Cu/Zn SOD in chloroplasts of Arabidopsis [16], tobacco [17,18], Chinese cabbage (Brassica campestris L. ssp. pekinensis cv. Tropical Pride) [19], and cotton [20] can enhance salinity tolerance through reducing ROS (Table 1). Similarly, tobacco plants with an overexpression of A ...
Potential Role of Sulfur-Containing Antioxidant Systems in Highly
... Species that are less reactive such as peroxides are much more selective in the targets that they damage, with modifications occurring to specific molecules at particular sites [20]. The factors determining the extent of damage to particular targets include: (a) the target concentration; (b) the rat ...
... Species that are less reactive such as peroxides are much more selective in the targets that they damage, with modifications occurring to specific molecules at particular sites [20]. The factors determining the extent of damage to particular targets include: (a) the target concentration; (b) the rat ...
Antifolding activity of hsp60 couples protein import into the
... be maintained in an unfolded conformation for both import into mitochondria and for bacterial secretion, this raises the question of how folding is regulated in case of proteins that have to undergo successive steps of membrane translocation. Do the imported proteins fold in the matrix and have to b ...
... be maintained in an unfolded conformation for both import into mitochondria and for bacterial secretion, this raises the question of how folding is regulated in case of proteins that have to undergo successive steps of membrane translocation. Do the imported proteins fold in the matrix and have to b ...
The ins and outs of sphingolipid synthesis
... site facing the cytoplasm (Figure 2). Studies of the mammalian enzyme originally suggested a single-pass transmembrane protein, with the C-terminus in the cytoplasm [9], but these data are not inconsistent with recent studies in yeast suggesting that the enzyme actually traverses the membrane three ...
... site facing the cytoplasm (Figure 2). Studies of the mammalian enzyme originally suggested a single-pass transmembrane protein, with the C-terminus in the cytoplasm [9], but these data are not inconsistent with recent studies in yeast suggesting that the enzyme actually traverses the membrane three ...
Opposing effects of target overexpression reveal drug mechanisms
... development. Despite the evolutionary and pharmacological importance of resistance by target overexpression, it remains unclear why this phenomenon is only seen for some drugs but not others. Here we address this question by examining antibiotics with known targets in Escherichia coli. For some drug ...
... development. Despite the evolutionary and pharmacological importance of resistance by target overexpression, it remains unclear why this phenomenon is only seen for some drugs but not others. Here we address this question by examining antibiotics with known targets in Escherichia coli. For some drug ...
Molecular Cloning and Expression Analysis of IgD in Nile
... comprise the major lymphoid organs of fish, yet bone marrow and lymph nodes constitute the immune organs of mammals. The fish immune system is far simpler than that of primates and rodents [8]. It is necessary to analyze IgD in teleosts, as it will provide a reference for further research in this fi ...
... comprise the major lymphoid organs of fish, yet bone marrow and lymph nodes constitute the immune organs of mammals. The fish immune system is far simpler than that of primates and rodents [8]. It is necessary to analyze IgD in teleosts, as it will provide a reference for further research in this fi ...
Limits of adaptation to high dietary protein intakes
... not suf®cient criterion. In addition there is the need to determine the extent to which the demand for a critical range of functions has been satis®ed. For the system as a whole the demand side is determined by the pattern and rate of protein synthesis and the utilisation of amino acids for other me ...
... not suf®cient criterion. In addition there is the need to determine the extent to which the demand for a critical range of functions has been satis®ed. For the system as a whole the demand side is determined by the pattern and rate of protein synthesis and the utilisation of amino acids for other me ...
Regulation of the heat stress response in Arabidopsis by
... separated on SDS-PAGE before Coomassie blue staining and autoradiography. M: molecular weight marker. (B) Similar assays as performed in (A) using in addition to GST-HsfA2 MBP and GST as controls. GST fusion proteins of HsfA2 is indicated by an arrow. ...
... separated on SDS-PAGE before Coomassie blue staining and autoradiography. M: molecular weight marker. (B) Similar assays as performed in (A) using in addition to GST-HsfA2 MBP and GST as controls. GST fusion proteins of HsfA2 is indicated by an arrow. ...
The KIebsieIIa pneumoniae cytochrome bd
... cytochrome bd’ has been extensive. Subunit I (cytochrome b5,&is encoded by cydA, and subunit I1 (which contains haem b,,, and haem d ) is encoded by cydB (Green et al., 1988). The cydAB genes are co-transcribed from a promoter upstream of cydA and are separated by 18 bp (Fang & Gennis, 1993). In sub ...
... cytochrome bd’ has been extensive. Subunit I (cytochrome b5,&is encoded by cydA, and subunit I1 (which contains haem b,,, and haem d ) is encoded by cydB (Green et al., 1988). The cydAB genes are co-transcribed from a promoter upstream of cydA and are separated by 18 bp (Fang & Gennis, 1993). In sub ...
Retina-Specific Expression of 5A11/Basigin-2, a
... indicated by ERG analyses.1,2 The architecture of the retina appears normal at eye opening, despite the electrophysiological deficiency.1 However, by 3 weeks of age (visual maturity in the mouse), the photoreceptor outer segments in the 5A11/ Basigin-null mouse retina appear less dense and shorter t ...
... indicated by ERG analyses.1,2 The architecture of the retina appears normal at eye opening, despite the electrophysiological deficiency.1 However, by 3 weeks of age (visual maturity in the mouse), the photoreceptor outer segments in the 5A11/ Basigin-null mouse retina appear less dense and shorter t ...
Immunohistochemical localization of fatty acid transporters and
... very long-chain fatty acids necessary for the synthesis of sebum lipids. FATP4 may play crucial roles in the development and maturation of both sebaceous glands and meibomian glands as well as in the production of sebum (10). Although FATPs have been reported to be present in the plasma membrane (15 ...
... very long-chain fatty acids necessary for the synthesis of sebum lipids. FATP4 may play crucial roles in the development and maturation of both sebaceous glands and meibomian glands as well as in the production of sebum (10). Although FATPs have been reported to be present in the plasma membrane (15 ...
The Old World monkey DAZ (Deleted in
... infertile men (3) led to the identification of three different AZF loci (AZFa, -b and -c). Microdeletions of AZFa–c could be detected in 5–15% of the infertile men, suggesting that the deleted regions of the Y chromosome contain genes essential for spermatogenesis (4,5). One candidate gene in the AZ ...
... infertile men (3) led to the identification of three different AZF loci (AZFa, -b and -c). Microdeletions of AZFa–c could be detected in 5–15% of the infertile men, suggesting that the deleted regions of the Y chromosome contain genes essential for spermatogenesis (4,5). One candidate gene in the AZ ...
Pharmacophore screening of the Protein Data Bank for specific
... (composed of two or more Phe, Tyr or Trp residues) that may additionally include an Asp or Glu residue;33 similarly, the Me-Lys binding site in the Ankyrin repeat of EHMT1 is formed by an aromatic cage containing an acidic residue.34 The PDB was therefore screened for pockets lined with aromatic an ...
... (composed of two or more Phe, Tyr or Trp residues) that may additionally include an Asp or Glu residue;33 similarly, the Me-Lys binding site in the Ankyrin repeat of EHMT1 is formed by an aromatic cage containing an acidic residue.34 The PDB was therefore screened for pockets lined with aromatic an ...