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This is the accepted version of the following article:
This is the accepted version of the following article:

... pathological conditions it can be abnormally phosphorylated which leads to aggregation and formation of neurofibrillary tangles representing pathological hallmark of Alzheimer's disease (AD). For its association with neurodegenerative diseases tau protein is intensively studied in various diagnostic ...
Chapter 2
Chapter 2

... imagine an almost limitless variety of peptides. For example, there are 2050 or slightly more than 1.12 ⫻ 1065 possible sequences for polypeptides with just 50 amino acid residues. ...
Answers to Scoring in Scrabble (English Word Play)
Answers to Scoring in Scrabble (English Word Play)

... Lysine rich region could also be a Lysine every other amino acid (KNKNKN) (AA[AG].{3}){4,} the .{3} specifies the intervening codon. To allow some but not all amino acids to occupy this “lysine rich region” we would have to write the regex for each one allowed and separate them with the OR symbol | ...
Dictionary of Interfaces in Proteins (DIP). Data Bank of
Dictionary of Interfaces in Proteins (DIP). Data Bank of

... procedures takes into account the atomic position of amino acid side-chains. As a consequence, these methods suffer from a loss of atomic details responsible for molecular recognition during folding, binding, etc. The vector-representation of secondary structures (Alesker et al., 1996) works very fa ...
Structure, function and regulation of pyruvate carboxylase
Structure, function and regulation of pyruvate carboxylase

... holocarboxylase synthetase is not clear. In 3T3-L1 mouse adipocytes, which contain very high levels of PC and ACC (EC 6.4.1.2), most holocarboxylase synthetase activity was detected in the cytosol, and only 30 % of activity was detected in the mitochondrial fraction [44]. A study with rat liver yiel ...
Molecular and metabolic changes of cherelle wilt of cacao and its
Molecular and metabolic changes of cherelle wilt of cacao and its

... cherelle wilt. Cherelles are susceptible to frosty pod rot caused by Moniliophthora roreri. We studied the cherelle wilt process and its impact on M. roreri infection using microscopic, metabolite, and gene expression analyses. Wilt was associated with increased levels of tricarboxylic acid cycle in ...
Preparing and Analyzing Expressed Sequence Tags (ESTs) Library
Preparing and Analyzing Expressed Sequence Tags (ESTs) Library

... and postnatal ESTs, respectively. According to the statistical results of PANTHER of high similarity prenatal and postnatal ESTs, 137 and 127 different putative genes were listed and classified, respectively, by GO terms. 39 prenatal ESTs and 37 postnatal EST were uncategorized. 9 different types of ...
The Glycerophosphoryl Diester
The Glycerophosphoryl Diester

... expressing a chimeric green fluorescence protein (GFP)– SHV3 protein with GFP attached to the C-terminal end of the SHV3 signal sequence. The root hair phenotype of shv3 was completely restored by transformation with this construct (data not shown), indicating that the fusion protein was fully funct ...
Homology among (βα) 8 Barrels: Implications for the Evolution of
Homology among (βα) 8 Barrels: Implications for the Evolution of

... be homologous (Jensen, 1976). Related to this view is that in which enzyme evolution has been driven by retention of catalytic mechanisms. There is good evidence to suggest that this has occurred within many protein families (Lawrence et al., 1997; Babbitt & Gerlt, 1997; Gerlt & Babbitt, 1998; Eklun ...
structural basis for thermal stability of thermophilic trmd proteins
structural basis for thermal stability of thermophilic trmd proteins

... The process of adapting and conforming to new environments through selection resulting in gradual changes which permit the survival of an organism is called evolution. Over the past few decades, scientists have been examining various extremophile genomes in an attempt to unravel structural conformat ...
Document
Document

... different components of the body[2]. Of course, the contribution of these components to whole body protein content differs with carcass protein being the largest contributor to whole body protein. Also, the contribution of the different parts to whole body protein changes over time with an increasin ...
SUPPLEMENTARY INFORMATION TABLE OF CONTENTS: 1
SUPPLEMENTARY INFORMATION TABLE OF CONTENTS: 1

... shift positions. This approach was used to generate a pseudogene for each DIRE encoding a single ingi/L1Tc-like sequence that contains numerous stop codons in most cases, as performed before [64,66]. The phylogenetic analysis was performed as before [64,66]. The reverse transcriptase (RT) amino acid ...
Methodology for predicting semantic annotations of protein
Methodology for predicting semantic annotations of protein

... and machine learning algorithms applied to protein sequence data sets. The second part comprises all the methods used in the proposed methodology for extracting and modeling of features derived of the protein sequences and the classification of proteins using those features. The third part of the th ...
Capabilities and limitations of gel electrophoresis for elemental
Capabilities and limitations of gel electrophoresis for elemental

... at low temperatures, allowing the detection of metals in a frozen gel. This can be particularly useful if the metal is not covalently bound to the protein and would not resist a chemical fixation (e.g., the first steps of silver- or coomassie blue-staining) of the proteins to prevent their diffusion ...
Milk Components - Great Lakes Regional Dairy Conference
Milk Components - Great Lakes Regional Dairy Conference

... • Blend price is a weighted average of 4 classes based on utilization in set order ...
Topology and Phosphorylation of Soybean Nodulin
Topology and Phosphorylation of Soybean Nodulin

... with six potential transmembrane domains and lacks an amino-terminal signal sequence. A full-length nodulin-26 cDNA and its various deletion derivatives were transcribed in vitro after linking them to bacteriophage T3 promoter. In vitro translation of these transcripts in a rabbit reticulocyte lysat ...
proteins: three-dimensional structure
proteins: three-dimensional structure

... be quite complex. The connection between two antiparallel strands may be just a small loop (Fig. 6-13a), but the link between tandem parallel strands must be a crossover connection that is out of the plane of the b sheet (Fig. 6-13b). The connecting link in either case can be extensive, often contai ...


... New knowledge can only be found by making use of the knowledge that already is there. In the following sections some general issues concerning enzymatic reactions and the regulation of enzymes are touched upon. Enzymes are proteins that catalyze chemical reactions. A chemical reaction can only occur ...
article in press - Biochemistry
article in press - Biochemistry

... NP006244) and g- (GenBank: NP997626) regulatory subunits. Mammals express several isoforms of each subunit, and different combinations produce enzymes with differing tissue and subcellular localization, as well as altered sensitivity to AMP (see Hardie, 2003 for a review of the known differences). H ...
Developmental Regulation of Genes Encoding Universal Stress
Developmental Regulation of Genes Encoding Universal Stress

... endodermal epithelium, a highly potent chemical barrier for protection against intruding microbes. Sequences associated with ESTs, SAGE Tags and microarray probes are functional genomic tools appropriate for dissecting gene functions.22 These tools can provide expression data to facilitate forward a ...
Pax8, a murine paired box gene expressed in the developing
Pax8, a murine paired box gene expressed in the developing

... the highly conserved region of the paired box oiPaxl is associated with the undulated mutant, characterized by malformations in the vertebral column (Balling et al. 1988). Another recently described murine paired box gene, Pax2, is expressed in restricted regions along the entire developing spinal c ...
Lecture 2 - cholesterol _CVS block
Lecture 2 - cholesterol _CVS block

... Most important animal steroid Mainitains membrane fluidity Has an insulating effect on nerve fibres Cholesterol is the parent molecule for – Bile acids and bile salts – Steroid hormones and – vitamin D3 ...
Statistical analysis of DNA microarray data
Statistical analysis of DNA microarray data

... using a known 3D structure of protein(s) with homologous sequence  Based on assumption that structure is more conserved than sequence  Important to use homologous proteins whose structures were determined by X-ray crystallography or NMR  Homology modeling is an important method since the number o ...
Yeast ING Protein Yeast Protein Human Ortholog Description of
Yeast ING Protein Yeast Protein Human Ortholog Description of

... MTRF1L ...
1 Phosphorus Nutrition Focus Issue
1 Phosphorus Nutrition Focus Issue

... Arabidopsis plants cultivated on RNA as their sole source of exogenous Pi grow just as well as Pi- ...
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Expression vector

An expression vector, otherwise known as an expression construct, is usually a plasmid or virus designed for protein expression in cells. The vector is used to introduce a specific gene into a target cell, and can commandeer the cell's mechanism for protein synthesis to produce the protein encoded by the gene. Expression vectors are the basic tools in biotechnology for the production of proteins.The plasmid is engineered to contain regulatory sequences that act as enhancer and promoter regions and lead to efficient transcription of the gene carried on the expression vector. The goal of a well-designed expression vector is the production of protein, and this may be achieve by the production of significant amount of stable messenger RNA, which can then be translated into protein. The protein may be expressed constitutively, or induced when necessary using an inducer. Escherichia coli is commonly used as the host for protein expression, other cell types however may also be used. An example of the use of expression vector is the production of insulin which is used for medical treatments of diabetes.
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