Synthetic Peptides as Antigens for Antibody Production
... generally be too hydrophobic to consider. The N-terminus of a protein can also prove to be a good candidate sequence, but in our experience is a less reliable choice than the C-terminus and may be modified or truncated. Regions with too high a charge or hydrophilicity are sometimes not as effective ...
... generally be too hydrophobic to consider. The N-terminus of a protein can also prove to be a good candidate sequence, but in our experience is a less reliable choice than the C-terminus and may be modified or truncated. Regions with too high a charge or hydrophilicity are sometimes not as effective ...
SUC1 and SUC2: two sucrose transporters from Arabidopsis
... either SUC1 or SUC2 under the control of the promoter of the yeast plasma membrane ATPase gene (PMA1) transport sucrose, and to a lesser extent also maltose, across their plasma membranes in an energy-dependent manner. The Ku-values for sucrose transport are 0.50 mM and 0.77 mM, respectively, and tr ...
... either SUC1 or SUC2 under the control of the promoter of the yeast plasma membrane ATPase gene (PMA1) transport sucrose, and to a lesser extent also maltose, across their plasma membranes in an energy-dependent manner. The Ku-values for sucrose transport are 0.50 mM and 0.77 mM, respectively, and tr ...
Complementary DNA Cloning, Messenger RNA
... nantly involved in detoxification reactions (1,2). They are found in all tissues and species, including bacteria (3), yeast (4), and plants (2). In higher organisms, three classes of GST protein, a, n, and 7T(1, 2), have been discriminated; a fourth class, 0, has been tentatively identified, althoug ...
... nantly involved in detoxification reactions (1,2). They are found in all tissues and species, including bacteria (3), yeast (4), and plants (2). In higher organisms, three classes of GST protein, a, n, and 7T(1, 2), have been discriminated; a fourth class, 0, has been tentatively identified, althoug ...
Supplementary Materials
... overexpression samples showed a value of 0.72. These significant correlations for both cases suggest that the underlying genetic contributions are the same across different ethanol concentrations. Modular analysis of fitness profiles The pathway-level analysis of the fitness profiles was performed ...
... overexpression samples showed a value of 0.72. These significant correlations for both cases suggest that the underlying genetic contributions are the same across different ethanol concentrations. Modular analysis of fitness profiles The pathway-level analysis of the fitness profiles was performed ...
Differential display proteomic analysis of Picea meyeri pollen
... Latrunculin B is a macrolide toxin purified from Latrunculia magnifica, a Red Sea sponge, which is capable of inhibiting actin cytoskeleton polymerization both in vitro and in vivo (Spector et al., 1983). LATB forms 1:1 complexes with actin monomers, thereby reducing the amount of Factin detectable ...
... Latrunculin B is a macrolide toxin purified from Latrunculia magnifica, a Red Sea sponge, which is capable of inhibiting actin cytoskeleton polymerization both in vitro and in vivo (Spector et al., 1983). LATB forms 1:1 complexes with actin monomers, thereby reducing the amount of Factin detectable ...
Chloroplast phosphoglycerate kinase from Euglena gracilis
... cytosolic and and chloroplast PGK protein sequences from Euglena gracilis with a representative sample of homologues from archaebacteria, eubacteria and eukaryotes was generated from LogDet distances based on a CLUSTALW alignment of the sequences (Fig. 4). As seen in many other analyses involving pr ...
... cytosolic and and chloroplast PGK protein sequences from Euglena gracilis with a representative sample of homologues from archaebacteria, eubacteria and eukaryotes was generated from LogDet distances based on a CLUSTALW alignment of the sequences (Fig. 4). As seen in many other analyses involving pr ...
Cox, G. Nutritional strategies to maximise recovery following
... It is worth reiterating that studies cited in this article are purely focused on enhancing post-exercise nutritional status, which for some athletes may not align itself with other nutritional issues they are juggling – for instance body weight reduction. In mentioning this, guidelines developed as ...
... It is worth reiterating that studies cited in this article are purely focused on enhancing post-exercise nutritional status, which for some athletes may not align itself with other nutritional issues they are juggling – for instance body weight reduction. In mentioning this, guidelines developed as ...
Johansson L, Gafvelin G, Arnér ES. Selenocysteine in proteins
... taking part in the catalysis. That at least some selenoproteins are of major importance for mammalian life was unequivocally demonstrated by the mouse knockout of the selenocysteinyl-tRNA gene (necessary for Sec incorporation and thereby selenoprotein synthesis), which show early embryonic lethality ...
... taking part in the catalysis. That at least some selenoproteins are of major importance for mammalian life was unequivocally demonstrated by the mouse knockout of the selenocysteinyl-tRNA gene (necessary for Sec incorporation and thereby selenoprotein synthesis), which show early embryonic lethality ...
Indian Agricultural Research Institute, New Delhi
... Indian Agricultural Research Institute, New Delhi ...
... Indian Agricultural Research Institute, New Delhi ...
Nutrition acquisition strategies during fungal infection of plants
... process. While great strides have been made in understanding how bacterial pathogen effectors are delivered through the type III secretion systems found in plant–pathogenic bacteria interactions, less is known at the molecular level of how fungi entrain host metabolism. For example, in the Cladospor ...
... process. While great strides have been made in understanding how bacterial pathogen effectors are delivered through the type III secretion systems found in plant–pathogenic bacteria interactions, less is known at the molecular level of how fungi entrain host metabolism. For example, in the Cladospor ...
Dietary protein for athletes - Inside Outside Wellness Center
... of opinion exists than between those who establish recommendations and the athletes themselves, particularly strength- and (or) power-training athletes. The published standards for dietary guidelines — the Dietary Reference Intakes (DRIs), assert that although protein is an essential nutrient, it is ...
... of opinion exists than between those who establish recommendations and the athletes themselves, particularly strength- and (or) power-training athletes. The published standards for dietary guidelines — the Dietary Reference Intakes (DRIs), assert that although protein is an essential nutrient, it is ...
Regulation of Gene Expression
... • more accurate regulation and more efficient. • Transcription and translation are separated : at different locations. ...
... • more accurate regulation and more efficient. • Transcription and translation are separated : at different locations. ...
Protein tyrosine phosphorylation in plants: more
... Determining protein phosphorylation sites by MS includes the following: mass spectra of phosphopeptides obtained by MS are aligned with a database of in silico fragmented (phospho)peptides, resulting in a top hit followed by lower ranking hits. Each hit comprises a peptide sequence, including the as ...
... Determining protein phosphorylation sites by MS includes the following: mass spectra of phosphopeptides obtained by MS are aligned with a database of in silico fragmented (phospho)peptides, resulting in a top hit followed by lower ranking hits. Each hit comprises a peptide sequence, including the as ...
Circadian Rhythm of Intestinal SucraseActivity in Rats
... ceases. This rhythm can be temporally shifted by altering the time of controlled feeding (5), but can be eliminated only by several days of starvation (2). Because most investigators have found a rise in enzyme activity 1 h before the scheduled beginning of feeding, many have postulated that it is t ...
... ceases. This rhythm can be temporally shifted by altering the time of controlled feeding (5), but can be eliminated only by several days of starvation (2). Because most investigators have found a rise in enzyme activity 1 h before the scheduled beginning of feeding, many have postulated that it is t ...
Journal of Microbiology and Biotechnology
... ATG codon to the downstream of the stop codon of the entire ipdC gene of P. polymyxa E681. The PCR product was isolated on agarose gel and cloned into pGEM-Teasy vector (Promega, Madison, WI, U.S.A.). The pGEM-Teasy::ipdC plasmid extracted from E. coli XL1-Blue was then digested with BamHI and EcoRI ...
... ATG codon to the downstream of the stop codon of the entire ipdC gene of P. polymyxa E681. The PCR product was isolated on agarose gel and cloned into pGEM-Teasy vector (Promega, Madison, WI, U.S.A.). The pGEM-Teasy::ipdC plasmid extracted from E. coli XL1-Blue was then digested with BamHI and EcoRI ...
Cell-Free Phospholipid Biosynthesis by Gene
... Both synthesized GPAT and LPAAT enzymes are active when coreconstituted in liposomes Having established that the full-length GPAT and LPAAT proteins can be synthesized in the PURE system and be incorporated in the membrane of liposomes we then explored the potential of mass spectrometry (MS) combine ...
... Both synthesized GPAT and LPAAT enzymes are active when coreconstituted in liposomes Having established that the full-length GPAT and LPAAT proteins can be synthesized in the PURE system and be incorporated in the membrane of liposomes we then explored the potential of mass spectrometry (MS) combine ...
Structure of the enzyme-acyl carrier protein (ACP) substrate
... good as might be expected. Another possible source of background would be inefficient removal of the first enzyme. To deal with this possibility, we (rather perversely) used the tag system having the highest known affinity, biotin-streptavidin. The BioH and BioC proteins were expressed as fusions to an ...
... good as might be expected. Another possible source of background would be inefficient removal of the first enzyme. To deal with this possibility, we (rather perversely) used the tag system having the highest known affinity, biotin-streptavidin. The BioH and BioC proteins were expressed as fusions to an ...
Protein for Athletes
... of muscle, it is estimated that 10 to 14 g of additional protein is needed each day,2 although others dispute this claim. In addition to possible growth of muscle, protein is a highly versatile nutrient and is involved in other functions that are crucial to sports performance: cell regulation, muscl ...
... of muscle, it is estimated that 10 to 14 g of additional protein is needed each day,2 although others dispute this claim. In addition to possible growth of muscle, protein is a highly versatile nutrient and is involved in other functions that are crucial to sports performance: cell regulation, muscl ...
Planta
... development of post-harvest practices to enhance fruit quality. Proteomics is becoming a powerful tool in plant research in the last few years. The development of state-of-the-art LC-MS/MS technology, Wne separation techniques, development of genomic, and ESTs databases for a variety of species and ...
... development of post-harvest practices to enhance fruit quality. Proteomics is becoming a powerful tool in plant research in the last few years. The development of state-of-the-art LC-MS/MS technology, Wne separation techniques, development of genomic, and ESTs databases for a variety of species and ...
Word
... is a highly reactive molecule that is toxic in its free form. How PLP levels are regulated and how ...
... is a highly reactive molecule that is toxic in its free form. How PLP levels are regulated and how ...
Metabolism of “surplus” amino acids
... For an adult in N balance, apart from small amounts of amino acids required for the synthesis of neurotransmitters, hormones, etc, an amount of amino acids almost equal to that absorbed from the diet can be considered to be “surplus” in that it will be catabolized. The higher diet-induced thermogene ...
... For an adult in N balance, apart from small amounts of amino acids required for the synthesis of neurotransmitters, hormones, etc, an amount of amino acids almost equal to that absorbed from the diet can be considered to be “surplus” in that it will be catabolized. The higher diet-induced thermogene ...
Dynamics of the trp Operon
... * Cluster of genes controlled by a single (?) feedback regulatory mechanism. ...
... * Cluster of genes controlled by a single (?) feedback regulatory mechanism. ...
Biocommunication in Plants
... called BraI/R, has been found. This system produces an AHL (3-oxo-C12-HSL) that is unique to the plant-associated Burkholderia and distinct from the CepI/R AHLs produced by pathogenic Burkholderia species. The BraI/R system appears to be involved in exopolysaccharide biosynthesis, but mutating braR ...
... called BraI/R, has been found. This system produces an AHL (3-oxo-C12-HSL) that is unique to the plant-associated Burkholderia and distinct from the CepI/R AHLs produced by pathogenic Burkholderia species. The BraI/R system appears to be involved in exopolysaccharide biosynthesis, but mutating braR ...
Protein phosphorylation in bacterial signal transduction
... also transiently phosphorylated on histidine residues. The PTS consists of a cascade of phosphotransferases that transmit the phosphate from phosphoenol pyruvate to the sugar that is being imported. The level of histidine phosphorylation in the cascade depends on the flux of incoming sugars and parti ...
... also transiently phosphorylated on histidine residues. The PTS consists of a cascade of phosphotransferases that transmit the phosphate from phosphoenol pyruvate to the sugar that is being imported. The level of histidine phosphorylation in the cascade depends on the flux of incoming sugars and parti ...
Stages of Translation (Biol 200 Sp2015): KEY Initiation
... Step 2: List the key concepts and/or main points that you have learned about your stage of translation. In your list include the following information: 1) the three steps in elongation and 2) the relationship between codons and anticodons Key concepts: Steps in elongation: 1) tRNA enters A site; an ...
... Step 2: List the key concepts and/or main points that you have learned about your stage of translation. In your list include the following information: 1) the three steps in elongation and 2) the relationship between codons and anticodons Key concepts: Steps in elongation: 1) tRNA enters A site; an ...