N-Terminal Intramolecularly Conserved Histidines of Three Domains
... luciferase molecule (5). The ORF (Figure 1) contains an N-terminal sequence of 111 amino acids, which is homologous to the N-terminal region of the substrate-binding protein, LBP. This is followed by three contiguous domains, designated D1, D2, and D3, which are homologous to one another. At the nuc ...
... luciferase molecule (5). The ORF (Figure 1) contains an N-terminal sequence of 111 amino acids, which is homologous to the N-terminal region of the substrate-binding protein, LBP. This is followed by three contiguous domains, designated D1, D2, and D3, which are homologous to one another. At the nuc ...
1 A Comparative, Double-blind, Triple Crossover Net Nitrogen
... Due to the fact that the methodology of the nitrogen balance is valid only when it is carried out in a accurate manner (8,9), the following precautions were taken during the study: a) To avoid or to reduce to a minimum the possible differences in the retention of N, caused by a determined dietetic s ...
... Due to the fact that the methodology of the nitrogen balance is valid only when it is carried out in a accurate manner (8,9), the following precautions were taken during the study: a) To avoid or to reduce to a minimum the possible differences in the retention of N, caused by a determined dietetic s ...
The GPI Transamidase Complex of Saccharomyces
... molecular masses of 50, 48, and 46 kDa (Benghezal et al., 1996).) After 4 h on glucose, the amount of Gpi8p is drastically reduced, and almost all Gpi8p is found within highmolecular-weight bands corresponding to apparent molecular masses of 430 – 650 kDa. The large smear observed in cells overexpre ...
... molecular masses of 50, 48, and 46 kDa (Benghezal et al., 1996).) After 4 h on glucose, the amount of Gpi8p is drastically reduced, and almost all Gpi8p is found within highmolecular-weight bands corresponding to apparent molecular masses of 430 – 650 kDa. The large smear observed in cells overexpre ...
Biochemistry, proteomics, and phosphoproteomics of plant
... from Arabidopsis suspension cells, a de-differentiated cell type that, when grown in darkness with sugar, is non-photosynthetic. For this reason and space considerations, this discussion of proteomics and post-translational modifications will focus mainly on Arabidopsis suspension cells and etiolate ...
... from Arabidopsis suspension cells, a de-differentiated cell type that, when grown in darkness with sugar, is non-photosynthetic. For this reason and space considerations, this discussion of proteomics and post-translational modifications will focus mainly on Arabidopsis suspension cells and etiolate ...
Title Detection of osteopontin as matrix protein in calcium
... because calcium ions are not involved in the formation of struvite stones as with uric acid stones, and therefore, OPN with affinity for calcium ions was not necessary. Another possibility is degradation of OPN in the formation process of struvite stones. Further studies are necessary. On the other ...
... because calcium ions are not involved in the formation of struvite stones as with uric acid stones, and therefore, OPN with affinity for calcium ions was not necessary. Another possibility is degradation of OPN in the formation process of struvite stones. Further studies are necessary. On the other ...
The synthesis of peptides and proteins containing non
... modifications can incorporate many different structural and functional features. This approach has been elegantly exploited in Jones’ systematic engineering of subtilisin mutants, which has also been reviewed comprehensively.7 In one example chemically modified subtilisin mutants were generated and ...
... modifications can incorporate many different structural and functional features. This approach has been elegantly exploited in Jones’ systematic engineering of subtilisin mutants, which has also been reviewed comprehensively.7 In one example chemically modified subtilisin mutants were generated and ...
Metabolic Pathway Flux Enhancement by Synthetic
... downstream enzyme (Conrado et al., 2008; Welch, 1977). This has been the subject of debate in early papers in which consecutive enzymes, b-galactosidase and galactose dehydrogenase, were tethered with a translational fusion, generating a higher product titer (Ljungcrantz et al., 1989). The authors s ...
... downstream enzyme (Conrado et al., 2008; Welch, 1977). This has been the subject of debate in early papers in which consecutive enzymes, b-galactosidase and galactose dehydrogenase, were tethered with a translational fusion, generating a higher product titer (Ljungcrantz et al., 1989). The authors s ...
Midwest climate summary - US Soybean Export Council
... regions, decreased slightly. Protein levels increased slightly in the Midsouth, but were significantly higher in the Southeast and East Coast region. Oil concentrations were lower throughout all of the regions, except the MidSouth, where there was a slight increase. As was the case in 2010, seed fro ...
... regions, decreased slightly. Protein levels increased slightly in the Midsouth, but were significantly higher in the Southeast and East Coast region. Oil concentrations were lower throughout all of the regions, except the MidSouth, where there was a slight increase. As was the case in 2010, seed fro ...
Protein structure
... Ab initio protein structure prediction Ab initio prediction can be performed when a protein has no detectable homologs. Protein folding is modeled based on global free-energy minimum estimates. The “Rosetta Stone” methods was applied to sequence families lacking known structures. For 80 of 131 prot ...
... Ab initio protein structure prediction Ab initio prediction can be performed when a protein has no detectable homologs. Protein folding is modeled based on global free-energy minimum estimates. The “Rosetta Stone” methods was applied to sequence families lacking known structures. For 80 of 131 prot ...
COMBINATION COMPRISING AT LEAST ONE AMINO ACID AND A
... Previously, leucine has been shown to function, not only as a protein building block, but also as an inducer of signal transduction pathways that modulate translation initiation. Our recent novel research suggests that all three of the BCAAs possess the ability to reduce protein degradation and enha ...
... Previously, leucine has been shown to function, not only as a protein building block, but also as an inducer of signal transduction pathways that modulate translation initiation. Our recent novel research suggests that all three of the BCAAs possess the ability to reduce protein degradation and enha ...
IdaPro® Milk Proteins - SupplySide Storefronts
... properties of the casein micelles in the milk proteins. Milk protein-bound calcium has also been shown to increase fat loss for people on calorie restricted diets by as much as 22% vs. proteins without bioavailable calcium.8 This is because the calcium bound to the casein micelle in milk protein is ...
... properties of the casein micelles in the milk proteins. Milk protein-bound calcium has also been shown to increase fat loss for people on calorie restricted diets by as much as 22% vs. proteins without bioavailable calcium.8 This is because the calcium bound to the casein micelle in milk protein is ...
The hnRNP C Proteins Contain a Nuclear Retention Sequence That
... All expression plasmids used in this study are derived from a modified form of pcDNA3 (Invitrogen, San Diego, CA) termed pcDNA3-myc, which has a myc epitope (MGEQKLISEEDL) just upstream of the EcoRI site (Siomi and Dreyfuss, 1995). Myc-C1 was made by PCR amplification of the C1 cDNA pHC12 (Swanson e ...
... All expression plasmids used in this study are derived from a modified form of pcDNA3 (Invitrogen, San Diego, CA) termed pcDNA3-myc, which has a myc epitope (MGEQKLISEEDL) just upstream of the EcoRI site (Siomi and Dreyfuss, 1995). Myc-C1 was made by PCR amplification of the C1 cDNA pHC12 (Swanson e ...
Enzyme changes in malnutrition - Journal of Clinical Pathology
... plasma albumin which is synthesized in the liver. The first enzyme to be studied from a nutritional standpoint was also a protein derived from the liver, namely, cholinesterase (EC 3.1.1.8). At about the same time McCance and his colleagues working on adults in Germany (Hutchinson, McCance, and Widd ...
... plasma albumin which is synthesized in the liver. The first enzyme to be studied from a nutritional standpoint was also a protein derived from the liver, namely, cholinesterase (EC 3.1.1.8). At about the same time McCance and his colleagues working on adults in Germany (Hutchinson, McCance, and Widd ...
Use of Gene Replacement Transformation to Elucidate
... also subjectto carboncatabolite repression (JOHNSTON 1987). Carbon repressionof the GAL genes is thought to operate throughseveral mechanisms such as direct action on the various GAL promoters, inhibition of activator binding and inhibition of galactose transport (JOHNSTON 1987). However, the mechan ...
... also subjectto carboncatabolite repression (JOHNSTON 1987). Carbon repressionof the GAL genes is thought to operate throughseveral mechanisms such as direct action on the various GAL promoters, inhibition of activator binding and inhibition of galactose transport (JOHNSTON 1987). However, the mechan ...
Identification of Surface Residues Involved in Protein
... rectly identified. With this level of success, predictions generated using this approach should be valuable for guiding experimental investigations into the roles of specific residues of a protein in its interaction with other proteins. Detailed examination of the predicted interface residues in th ...
... rectly identified. With this level of success, predictions generated using this approach should be valuable for guiding experimental investigations into the roles of specific residues of a protein in its interaction with other proteins. Detailed examination of the predicted interface residues in th ...
Translation
... pairing with the mRNA initiation codon (AUG). A region of mRNA upstream of the initiation codon, the Shine-Dalgarno sequence, base pairs with the 3' end of the 16S rRNA. This positions the 30S ribosomal subunit in relation to the initiation codon. As the large ribosomal subunit joins the complex, ...
... pairing with the mRNA initiation codon (AUG). A region of mRNA upstream of the initiation codon, the Shine-Dalgarno sequence, base pairs with the 3' end of the 16S rRNA. This positions the 30S ribosomal subunit in relation to the initiation codon. As the large ribosomal subunit joins the complex, ...
Supplemental Materials
... If there is any difference in the colored beads between the normal and mutated protein, write the difference here ____________________________________________________________. 5) Now we need to fold this amino acid sequence into a 3D shape for the hemoglobin protein to function properly. How do you ...
... If there is any difference in the colored beads between the normal and mutated protein, write the difference here ____________________________________________________________. 5) Now we need to fold this amino acid sequence into a 3D shape for the hemoglobin protein to function properly. How do you ...
Formate Dehydrogenase, an Enzyme of Anaerobic Metabolism, Is
... (Lamzin et al., 1992) and the formate-binding site from Arg-285 are indicated. The sequences were highly conserved between barley and potato in contrast to the sequences at the N terminus, which displayed very low sequence homology. Southern Hybridization Analysis The copy number of Fdh in barley wa ...
... (Lamzin et al., 1992) and the formate-binding site from Arg-285 are indicated. The sequences were highly conserved between barley and potato in contrast to the sequences at the N terminus, which displayed very low sequence homology. Southern Hybridization Analysis The copy number of Fdh in barley wa ...
Full-Text PDF
... catalytically-competent orientation. The crystal structure of MTG revealed that this triad is not conserved; rather, it was proposed that MTG uses a cysteine protease-like mechanism in which Asp255 plays the role of the histidine residue in factor XIII-like TGases [15]. Of the two, MTG is more robus ...
... catalytically-competent orientation. The crystal structure of MTG revealed that this triad is not conserved; rather, it was proposed that MTG uses a cysteine protease-like mechanism in which Asp255 plays the role of the histidine residue in factor XIII-like TGases [15]. Of the two, MTG is more robus ...
Anti-Human Ciliary Neurotrophic Factor (CNTF) (C3960)
... factor for developing ciliary ganglionic neurons.2 ...
... factor for developing ciliary ganglionic neurons.2 ...
1 This exam consists of 5 pages and 15
... Ig Fold – two 3-4 stranded β-sheets that are stabilized by H-bonds within the sheet, a disulfide bond holds the two sheets together. Choice B: A disulfide bond is a S-S bond between two Cysteine residues in a protein. They reduce the conformational entropy of the unfolded state, thus making it less ...
... Ig Fold – two 3-4 stranded β-sheets that are stabilized by H-bonds within the sheet, a disulfide bond holds the two sheets together. Choice B: A disulfide bond is a S-S bond between two Cysteine residues in a protein. They reduce the conformational entropy of the unfolded state, thus making it less ...
Provitamin A Accumulation in Cassava (Manihot
... Sequencing of the two cassava full-length PSY cDNAs (PSY1 and PSY2) revealed the occurrence of single nucleotide polymorphisms (SNPs) in PSY2 (see Supplemental Figure 1 online). Two of these, SNP1 and SNP2, were present in the Y cultivar, defining the alleles PSY2-Y-1 and PSY2-Y-2 (Figure 3), while ...
... Sequencing of the two cassava full-length PSY cDNAs (PSY1 and PSY2) revealed the occurrence of single nucleotide polymorphisms (SNPs) in PSY2 (see Supplemental Figure 1 online). Two of these, SNP1 and SNP2, were present in the Y cultivar, defining the alleles PSY2-Y-1 and PSY2-Y-2 (Figure 3), while ...
記錄 編號 3862 狀態 NC090FJU00112010 助教 查核 索書 號 學校
... determined by PCR-restriction fragment length polymorphism analysis of the hrp gene region. Appl. Environ. Microbiol. 65: 2184-2194. Reimmann, C., and Haas, D. 1987. Mode of replicon fusion mediated by the duplicated insertion sequence IS21 in Escherichia coli. Genetics 115: 619-625. Reimmann, C., a ...
... determined by PCR-restriction fragment length polymorphism analysis of the hrp gene region. Appl. Environ. Microbiol. 65: 2184-2194. Reimmann, C., and Haas, D. 1987. Mode of replicon fusion mediated by the duplicated insertion sequence IS21 in Escherichia coli. Genetics 115: 619-625. Reimmann, C., a ...
STRUCTURE AND FUNCTION
... GlnK1 with No Effectors. Four trimers of the GlnK1 protein formed a cluster when crystallized with no effectors bound (Figure 5). Each trimer contained three T-Loops which differed in conformation. Six out the 12 T-loops in the four trimers were disordered, the remaining six were well-defined and in ...
... GlnK1 with No Effectors. Four trimers of the GlnK1 protein formed a cluster when crystallized with no effectors bound (Figure 5). Each trimer contained three T-Loops which differed in conformation. Six out the 12 T-loops in the four trimers were disordered, the remaining six were well-defined and in ...
State of the Art Manufacturing of Protein Hydrolysates
... the use of egg albumin was published in 1882 by Naegeli, which he referred to as “peptone,” a term still used today for some products. However, later it was discovered that peptones derived from the partial digestion (hydrolysis) of proteins would furnish organic nitrogen in a more available form (P ...
... the use of egg albumin was published in 1882 by Naegeli, which he referred to as “peptone,” a term still used today for some products. However, later it was discovered that peptones derived from the partial digestion (hydrolysis) of proteins would furnish organic nitrogen in a more available form (P ...