SDS-PAGE strongly overestimates the molecular
... domains are not to be expected and emerging partial amino acid sequence data centering on CNBr fragments (unpub~ished~ afso favor this prediction. The molecular mass of H cannot be predicted as clearly. Although both gel filtration and centrifugation point to values some 30-40% lower than SDS-PAGE ( ...
... domains are not to be expected and emerging partial amino acid sequence data centering on CNBr fragments (unpub~ished~ afso favor this prediction. The molecular mass of H cannot be predicted as clearly. Although both gel filtration and centrifugation point to values some 30-40% lower than SDS-PAGE ( ...
The monocarboxylate transporter family
... MCT isoforms. This was confirmed when Christine Kim Garcia in the laboratory of Goldstein and Brown cloned a second isoform which transported lactate and pyruvate when expressed in insect Sf9 cells (25). They named this isoform MCT2 and reported that it had a higher affinity for substrates than MCT1. ...
... MCT isoforms. This was confirmed when Christine Kim Garcia in the laboratory of Goldstein and Brown cloned a second isoform which transported lactate and pyruvate when expressed in insect Sf9 cells (25). They named this isoform MCT2 and reported that it had a higher affinity for substrates than MCT1. ...
Photosynthesis genes in marine viruses yield proteins during host
... the phage genome is a function of photosynthesis. We thus propose that the phage genes are functional in photosynthesis and that they may be increasing phage fitness by supplementing the host production of these proteins. Photosynthesis in cyanobacteria, algae and plants requires two photosystems (d ...
... the phage genome is a function of photosynthesis. We thus propose that the phage genes are functional in photosynthesis and that they may be increasing phage fitness by supplementing the host production of these proteins. Photosynthesis in cyanobacteria, algae and plants requires two photosystems (d ...
Descriptions of translation related genes that
... Small cytosolic ribosomal protein, involved in translation initiation and metabolic processes Component of heteroheptameric complexes, involved in mRNA processing Ribonucleoprotein involved in regulating mRNA translation, transport, processing PUF protein family member, involved in DNA metabolism an ...
... Small cytosolic ribosomal protein, involved in translation initiation and metabolic processes Component of heteroheptameric complexes, involved in mRNA processing Ribonucleoprotein involved in regulating mRNA translation, transport, processing PUF protein family member, involved in DNA metabolism an ...
Comparison of Rumen Amino Acid Protection Technologies
... Glutamic acid Glutamine Glycine Proline Serine Tyrosine ...
... Glutamic acid Glutamine Glycine Proline Serine Tyrosine ...
Transcriptome profiling identifies candidate genes associated with
... with each of the Unigenes was summed to provide the total number of reads for this gene. When multiple Unigenes were assigned to a single gene, their alignment was usually non-overlapping, i.e., there were gaps in coverage of the reference coding sequence. A nomenclature was developed for sulfate tr ...
... with each of the Unigenes was summed to provide the total number of reads for this gene. When multiple Unigenes were assigned to a single gene, their alignment was usually non-overlapping, i.e., there were gaps in coverage of the reference coding sequence. A nomenclature was developed for sulfate tr ...
Molecular cloning, characterization and gene expression of an
... Catalase is a very highly conserved enzyme that has been identified from numerous species including bacteria, fungi, plants and animals. This enzyme is ubiquitous and present in archaea [22], prokaryotes and eukaryotes [32e35]. To date, much information about the structure and regulation of catalase ...
... Catalase is a very highly conserved enzyme that has been identified from numerous species including bacteria, fungi, plants and animals. This enzyme is ubiquitous and present in archaea [22], prokaryotes and eukaryotes [32e35]. To date, much information about the structure and regulation of catalase ...
A Major Surface Protein on Group A Streptococci Is a
... Dot-Blot Immunoassay to Determine the Location of the 39-kD Protein. The surface location of 39-kD protein was determined with the monospecific antibodies using a bacterial dot-blot immunoassay as previously described (31, 40). Essentially, an overnight culture of strain D471 was adjusted to ODium 1 ...
... Dot-Blot Immunoassay to Determine the Location of the 39-kD Protein. The surface location of 39-kD protein was determined with the monospecific antibodies using a bacterial dot-blot immunoassay as previously described (31, 40). Essentially, an overnight culture of strain D471 was adjusted to ODium 1 ...
Session 377 Visual cycle and phototransduction
... Purpose: The macular carotenoids, lutein and zeaxanthin, can play a protective role in ocular diseases such as age-related macular degeneration and cataract. β-Carotene, another abundant carotenoid present in the human serum could serve as a vitamin A (retinol) precursor. Carotenoids are lipophilic ...
... Purpose: The macular carotenoids, lutein and zeaxanthin, can play a protective role in ocular diseases such as age-related macular degeneration and cataract. β-Carotene, another abundant carotenoid present in the human serum could serve as a vitamin A (retinol) precursor. Carotenoids are lipophilic ...
Sulfur Metabolism in Escherichia coli and Related Bacteria: Facts
... 3'OH position of APS, being transformed into 3'phosphoadenosine phosphosulfate (PAPS). One can think that the raison d’être of this reaction, which from ATP produces PAPS and ADP, is a supplementary means to pull the reaction toward the anabolic direction. In fact, not only does the 3' phosphate of ...
... 3'OH position of APS, being transformed into 3'phosphoadenosine phosphosulfate (PAPS). One can think that the raison d’être of this reaction, which from ATP produces PAPS and ADP, is a supplementary means to pull the reaction toward the anabolic direction. In fact, not only does the 3' phosphate of ...
Roles of phosphatidate phosphatase enzymes in lipid metabolism
... (DAG) and inorganic phosphate [1] (Figure 1). PAP enzymes have roles in both the synthesis of lipids and the generation or degradation of lipid-signaling molecules in eukaryotic cells. They are classified as either Mg2+-dependent (referred to as PAP1 enzymes) or Mg2+independent (PAP2 or lipid phosph ...
... (DAG) and inorganic phosphate [1] (Figure 1). PAP enzymes have roles in both the synthesis of lipids and the generation or degradation of lipid-signaling molecules in eukaryotic cells. They are classified as either Mg2+-dependent (referred to as PAP1 enzymes) or Mg2+independent (PAP2 or lipid phosph ...
5 x buffer (50TB 25 7 - American Journal of Physiology
... muscle as a source of the enriched GLUT-4 donor membrane fraction. We tested whether cytosolic protein fractions prepared from other tissue sources (liver, brain, blood plasma) than rat skeletal muscle could induce in vitro GLUT-4 transfer. We found that human blood plasma and to a lesser extent bra ...
... muscle as a source of the enriched GLUT-4 donor membrane fraction. We tested whether cytosolic protein fractions prepared from other tissue sources (liver, brain, blood plasma) than rat skeletal muscle could induce in vitro GLUT-4 transfer. We found that human blood plasma and to a lesser extent bra ...
Two Genes with Similarity to Bacterial Response Regulators Are
... response regulator receiver domain at the C terminus. These proteins have been referred to as hybrid kinases. One exception is ERS (for ethylene response sensor), a member of the Arabidopsis ETR gene family, which is comprised solely of a histidine kinase and a putative ethylene binding domain. Seve ...
... response regulator receiver domain at the C terminus. These proteins have been referred to as hybrid kinases. One exception is ERS (for ethylene response sensor), a member of the Arabidopsis ETR gene family, which is comprised solely of a histidine kinase and a putative ethylene binding domain. Seve ...
Plasma Membrane Transporter Protein Mutations
... than one glucose transporter is expressed by most cells. The jejunal epithelial cell uses SGLT1 to concentrate glucose from its luminal surface into the cytosol, then effluxes glucose at its basal-lateral surfaces through GLUT2. GLUT2 is also involved in regulating the amount of glucose transported ...
... than one glucose transporter is expressed by most cells. The jejunal epithelial cell uses SGLT1 to concentrate glucose from its luminal surface into the cytosol, then effluxes glucose at its basal-lateral surfaces through GLUT2. GLUT2 is also involved in regulating the amount of glucose transported ...
Determination and Quantification of Molecular Interactions in Protein
... film formation. Although protein films have relatively poor water vapor barriers, due to their hydrophilic nature, they have excellent gas barrier properties [14,17–24]. A benefit of proteins is also their availability in high quantities. For example, whey protein is a by-product of cheese productio ...
... film formation. Although protein films have relatively poor water vapor barriers, due to their hydrophilic nature, they have excellent gas barrier properties [14,17–24]. A benefit of proteins is also their availability in high quantities. For example, whey protein is a by-product of cheese productio ...
Critical care: Meeting protein requirements without overfeeding energy
... international guidelines. We determined protein adequacy of current feed prescription in a ‘baseline’ audit and then the effect of added protein in a pilot ‘supplementation’ audit. The primary outcome was the difference between protein prescription and the local guidelines on days 1e3. Secondary out ...
... international guidelines. We determined protein adequacy of current feed prescription in a ‘baseline’ audit and then the effect of added protein in a pilot ‘supplementation’ audit. The primary outcome was the difference between protein prescription and the local guidelines on days 1e3. Secondary out ...
1 - Plant Research International
... exposed to radioactive intermediates followed by extraction and HPLC analysis to establish where in the cell the different pathways operate and if there is a site of synthesis with a separate site of accumulation. Sulphur Biochemistry Workpackage: Genes for CSO synthesis The genes responsible for th ...
... exposed to radioactive intermediates followed by extraction and HPLC analysis to establish where in the cell the different pathways operate and if there is a site of synthesis with a separate site of accumulation. Sulphur Biochemistry Workpackage: Genes for CSO synthesis The genes responsible for th ...
... Bonus (2 pts). Where could you find glycine residues on the Ramachandran plot? Why? The unfavorable regions are due to repulsive van der Waals interactions with the beta carbon. Since glycine has no beta carbon it could be found almost anywhere on the plot. Note: glycine has phi and psi angles and c ...
Protein
... peptidases digest all the remaining dipeptides and tripeptides into individual amino acids for absorption into the bloodstream. Undigested protein Any parts of proteins that are not digested and absorbed in the small intestine continue on through the large intestine. People with celiac disease, for ...
... peptidases digest all the remaining dipeptides and tripeptides into individual amino acids for absorption into the bloodstream. Undigested protein Any parts of proteins that are not digested and absorbed in the small intestine continue on through the large intestine. People with celiac disease, for ...
Exploring Mouse Protein Function via Multiple Approaches
... binary (i.e., 1 indicates interaction and 0 indicates no interaction). Additionally, the interaction between proteins can be strong or weak. The STRING database [37] is a protein interaction repository that characterizes each interaction into a weight value based on eight different lines of evidence ...
... binary (i.e., 1 indicates interaction and 0 indicates no interaction). Additionally, the interaction between proteins can be strong or weak. The STRING database [37] is a protein interaction repository that characterizes each interaction into a weight value based on eight different lines of evidence ...
Bioinformatics: Molecular Computational Tools (Module II)
... these resources are accessible to scientists (including you) via the Internet. Using the available databases and sequence analysis software, one can obtain a vast amount of information about a gene’s structure, its encoded message, and the putative function of its encoded protein. This lab is the be ...
... these resources are accessible to scientists (including you) via the Internet. Using the available databases and sequence analysis software, one can obtain a vast amount of information about a gene’s structure, its encoded message, and the putative function of its encoded protein. This lab is the be ...
bioinformatics module ii - Tetrahymena Genome Database
... these resources are accessible to scientists (including you) via the Internet. Using the available databases and sequence analysis software, one can obtain a vast amount of information about a gene’s structure, its encoded message, and the putative function of its encoded protein. This lab is the be ...
... these resources are accessible to scientists (including you) via the Internet. Using the available databases and sequence analysis software, one can obtain a vast amount of information about a gene’s structure, its encoded message, and the putative function of its encoded protein. This lab is the be ...
Translocation of proteins across the cell envelope of Gram
... the industrial production of enzymes that occur naturally in these organisms and that are secreted into the supernatant in large amounts. They may also function as excellent hosts for the secretory production of heterologous proteins. Indeed, Bacilli can be used successfully for this purpose, but so ...
... the industrial production of enzymes that occur naturally in these organisms and that are secreted into the supernatant in large amounts. They may also function as excellent hosts for the secretory production of heterologous proteins. Indeed, Bacilli can be used successfully for this purpose, but so ...
Supplemental Text
... GSSG levels similar to or even higher than APAP alone. This indicated that the delayed GSH treatment did not prevent the mitochondrial oxidant stress, which is presumably initiated by binding of NAPQI to mitochondrial proteins.15 These data provided strong evidence that the delayed treatment with G ...
... GSSG levels similar to or even higher than APAP alone. This indicated that the delayed GSH treatment did not prevent the mitochondrial oxidant stress, which is presumably initiated by binding of NAPQI to mitochondrial proteins.15 These data provided strong evidence that the delayed treatment with G ...