Interaction interfaces of protein domains are not topologically
... number of interactions, thus acting as hubs of an interaction network, tend to evolve more slowly than the bulk of the other proteins in the genome. Since the tertiary fold and gross functionality are preserved in several remote homologues [referred to as “superfamily” according to the Structural Cl ...
... number of interactions, thus acting as hubs of an interaction network, tend to evolve more slowly than the bulk of the other proteins in the genome. Since the tertiary fold and gross functionality are preserved in several remote homologues [referred to as “superfamily” according to the Structural Cl ...
Proteolytic processing of the auxiliary subunit is crucial - HAL
... Before investigating the functional relevance of the proteolytic cleavage of the auxiliary subunit on neuronal recombinant CaV channel activity, it was necessary to delimit the putative proteolytic site within the protein. Initial N-terminal sequencing studies on the rabbit skeletal muscle CaV c ...
... Before investigating the functional relevance of the proteolytic cleavage of the auxiliary subunit on neuronal recombinant CaV channel activity, it was necessary to delimit the putative proteolytic site within the protein. Initial N-terminal sequencing studies on the rabbit skeletal muscle CaV c ...
Dream Protein - Greens First
... Amino Acids are the chemical units or commonly known as the building blocks that make up proteins. Because they contain about 16 percent nitrogen, this is what distinguishes them from the other two basic nutrients, sugars and fatty acids. Protein provides the structure for all living things, necessa ...
... Amino Acids are the chemical units or commonly known as the building blocks that make up proteins. Because they contain about 16 percent nitrogen, this is what distinguishes them from the other two basic nutrients, sugars and fatty acids. Protein provides the structure for all living things, necessa ...
A General Method for the Determination of the Carboxyl
... The general principle behind the isolation procedure is similar to that described by Furka et al. (6). When a protein is digested with a proteolytic enzyme, each bond cleaved generates a new amino and a new carboxyl group. If the digestion is performed on a protein in which all carboxyl groups have ...
... The general principle behind the isolation procedure is similar to that described by Furka et al. (6). When a protein is digested with a proteolytic enzyme, each bond cleaved generates a new amino and a new carboxyl group. If the digestion is performed on a protein in which all carboxyl groups have ...
Supplementary information for Ronshaugen, McGinnis
... determined for the wild type A1 segments. Identically treated embryos were then stained with a rat monoclonal anti-HA antibody and the mean luminosity with this antibody in the T2 segment was determined. All other transgenic lines were similarly assayed using the anti-HA antibody. These were selecte ...
... determined for the wild type A1 segments. Identically treated embryos were then stained with a rat monoclonal anti-HA antibody and the mean luminosity with this antibody in the T2 segment was determined. All other transgenic lines were similarly assayed using the anti-HA antibody. These were selecte ...
Differential Accumulation Pattern of Met-rich beta
... sativa and 10 ug of total RNA from M. truncatula was seperated on the same 1.0% agarose formaldehyde gel and subjected to gel blot analysis using a 690 bp b-zein fragment as a probe. Blots were also hybridized to M. sativa Glutamine Synthase (GS2) probe to check the levels of GS2 transcript in M. sa ...
... sativa and 10 ug of total RNA from M. truncatula was seperated on the same 1.0% agarose formaldehyde gel and subjected to gel blot analysis using a 690 bp b-zein fragment as a probe. Blots were also hybridized to M. sativa Glutamine Synthase (GS2) probe to check the levels of GS2 transcript in M. sa ...
VTC4 Is a Bifunctional Enzyme That Affects
... both pathways are affected, we isolated three independent T-DNA insertion mutants. Seeds for vtc4-2 (SAIL_105_D11), vtc4-3 (SAIL_843_G10), and vtc4-4 (SALK_077222) were identified from the SALK T-DNA mutant database and were verified by sequencing of amplified PCR products. The vtc4-2 mutant contain ...
... both pathways are affected, we isolated three independent T-DNA insertion mutants. Seeds for vtc4-2 (SAIL_105_D11), vtc4-3 (SAIL_843_G10), and vtc4-4 (SALK_077222) were identified from the SALK T-DNA mutant database and were verified by sequencing of amplified PCR products. The vtc4-2 mutant contain ...
MASE1 and MASE2: Two Novel Integral Membrane Sensory Domains
... retxena-Belandia and Engelman, 2001]. The stable structure of the whole domain is probably maintained by the salt bridges between terminal parts of different helices, formed by poorly conserved charged residues (e.g., Asp123, Arg-109). Domain architectures of the MASE1-containing proteins show signi ...
... retxena-Belandia and Engelman, 2001]. The stable structure of the whole domain is probably maintained by the salt bridges between terminal parts of different helices, formed by poorly conserved charged residues (e.g., Asp123, Arg-109). Domain architectures of the MASE1-containing proteins show signi ...
Characterization of Saccharomyces cerevisiae deficient in
... expression of PLD activity in yeast. Cells lacking expression of this gene show a complete lack of PLD activity in our in itro assay system. While it is possible that yeast expresses other forms of PLD that are not detected by this particular assay, this seems unlikely in view of the fact that a PL ...
... expression of PLD activity in yeast. Cells lacking expression of this gene show a complete lack of PLD activity in our in itro assay system. While it is possible that yeast expresses other forms of PLD that are not detected by this particular assay, this seems unlikely in view of the fact that a PL ...
Plant Lipoxygenases. Physiological and Molecular Features
... (E)-2-hexenal induces a subset of JA-induced genes associated with the defense response, but fails to induce some other JA-responsive genes (Bate and Rothstein, 1998). This indicates that different signals from the LOX-derived pathways are mediators in the wounding response. In this respect, it is i ...
... (E)-2-hexenal induces a subset of JA-induced genes associated with the defense response, but fails to induce some other JA-responsive genes (Bate and Rothstein, 1998). This indicates that different signals from the LOX-derived pathways are mediators in the wounding response. In this respect, it is i ...
Genomic analysis and comparison of very virulent infectious bursal
... proteins forming the viral capsid. VP2 forming the outer capsid while VP3 formed the inner one. The primary cleavage site of the polyprotein had been recognized. The cleavage of preVP2-VP4 junction is believed to happen at Ala-512 and Ala-513 and VP4-VP3 junction is at Ala-755 and Ala-756 [9]. The p ...
... proteins forming the viral capsid. VP2 forming the outer capsid while VP3 formed the inner one. The primary cleavage site of the polyprotein had been recognized. The cleavage of preVP2-VP4 junction is believed to happen at Ala-512 and Ala-513 and VP4-VP3 junction is at Ala-755 and Ala-756 [9]. The p ...
DNA Specificity of the Bicoid Activator Protein Is Determined by
... proteins distinguish between these sites using residues in the recognition helix which differ, and are in positions corresponding to those important for repressor-DNA recognition. In this study, we employed fusion proteins that contain the DNA binding domains of the bacterial LexA repressor or the y ...
... proteins distinguish between these sites using residues in the recognition helix which differ, and are in positions corresponding to those important for repressor-DNA recognition. In this study, we employed fusion proteins that contain the DNA binding domains of the bacterial LexA repressor or the y ...
7.1 The lac Operon
... partially purifying the lac repressor. The most sensitive assay available to them was binding a labeled synthetic inducer (isopropylthiogalactoside, or IPTG) to the repressor. But, with a crude extract of wild-type cells, the repressor was in such low concentration that this assay could not detect i ...
... partially purifying the lac repressor. The most sensitive assay available to them was binding a labeled synthetic inducer (isopropylthiogalactoside, or IPTG) to the repressor. But, with a crude extract of wild-type cells, the repressor was in such low concentration that this assay could not detect i ...
emboj200897-sup
... substrate (hence the autokinase activity is measured), it is important to control the initial phosphorylation level of the samples. Supplementary Figure 3 shows a similar starting phosphorylation level for all wild type Etk and mutant samples used in this study. The fully dephosphorylated protein sa ...
... substrate (hence the autokinase activity is measured), it is important to control the initial phosphorylation level of the samples. Supplementary Figure 3 shows a similar starting phosphorylation level for all wild type Etk and mutant samples used in this study. The fully dephosphorylated protein sa ...
SARS: Severe Acute Respiratory Syndrome
... currently known about the disease, breakthroughs occur on a daily basis concerning SARS. The disease is caused by a virus that invades the lungs of a human, causing severe pneumonia-like symptoms. Viruses are able to change their DNA, or mutate, and by doing so, are able to become resistant to many ...
... currently known about the disease, breakthroughs occur on a daily basis concerning SARS. The disease is caused by a virus that invades the lungs of a human, causing severe pneumonia-like symptoms. Viruses are able to change their DNA, or mutate, and by doing so, are able to become resistant to many ...
Understanding conserved amino acids in proteins
... is the amino acid type at the position i = 1; : : : ; N . U (i ; j ) is the corresponding element of the matrix of pairwise interactions between amino acids i and j . ij is the element of the contact matrix that is deCned to be 1 if there is a contact between amino acids i and j, and is 0 other ...
... is the amino acid type at the position i = 1; : : : ; N . U (i ; j ) is the corresponding element of the matrix of pairwise interactions between amino acids i and j . ij is the element of the contact matrix that is deCned to be 1 if there is a contact between amino acids i and j, and is 0 other ...
pdf
... he development of methods to encode unnatural amino acids (UAAs) with novel properties genetically in living cells has significantly enhanced our ability to manipulate protein structure and function (1). Central to this system is a unique tRNA/aminoacyl-tRNA synthetase (aaRS) pair, orthogonal to the ...
... he development of methods to encode unnatural amino acids (UAAs) with novel properties genetically in living cells has significantly enhanced our ability to manipulate protein structure and function (1). Central to this system is a unique tRNA/aminoacyl-tRNA synthetase (aaRS) pair, orthogonal to the ...
Towards the construction of Escherichia coli cell
... Cell-free protein systems are described as the in vitro expression of recombinant proteins without the use of living cells. This approach uses a cell lysate containing a wide array of biological and chemical components for transcription, translation, protein folding, and energy metabolism; all requi ...
... Cell-free protein systems are described as the in vitro expression of recombinant proteins without the use of living cells. This approach uses a cell lysate containing a wide array of biological and chemical components for transcription, translation, protein folding, and energy metabolism; all requi ...
Caenorhabditis elegans unc-60 gene encodes
... vertebrate Z lines, and interdigitate with the thick filaments which are centrally stacked in the sarcomere. An electron-dense material analogous to the vertebrate M line is also present. While there are also significant differences, the similarities to vertebrate muscle make C. eleoans an attractiv ...
... vertebrate Z lines, and interdigitate with the thick filaments which are centrally stacked in the sarcomere. An electron-dense material analogous to the vertebrate M line is also present. While there are also significant differences, the similarities to vertebrate muscle make C. eleoans an attractiv ...
Outsmart Cancer, Eliminate Poisonous Toxins, Boost Antioxidant
... For the cancer patient, whey protein powder can be a lifesaving food. It is well known that cancer aggressively consumes proteins, leaving the rest of the body to starving for optimal amounts of this vital nutrient. Many anti-cancer diets have found that a mostly vegetarian diet is essential to heal ...
... For the cancer patient, whey protein powder can be a lifesaving food. It is well known that cancer aggressively consumes proteins, leaving the rest of the body to starving for optimal amounts of this vital nutrient. Many anti-cancer diets have found that a mostly vegetarian diet is essential to heal ...
Identifying Importance of Amino Acids for Protein
... Understanding the mechanisms of protein folding is also crucial for deciphering the imprints of evolution on protein sequence and structural spaces. For example, some positions along the sequence in a set of structurally similar nonhomologous proteins are more conserved in the course of evolution th ...
... Understanding the mechanisms of protein folding is also crucial for deciphering the imprints of evolution on protein sequence and structural spaces. For example, some positions along the sequence in a set of structurally similar nonhomologous proteins are more conserved in the course of evolution th ...
Arabidopsis Genes Encoding Components of the
... homolog. One possible explanation for the occurrence of these presumably unexpressed regions of similarity is that these short open reading frames are examples of the evolutionary process of exon shuffling in progress. In the case of AtToc75-IV, the region of similarity extends for approximately 50% ...
... homolog. One possible explanation for the occurrence of these presumably unexpressed regions of similarity is that these short open reading frames are examples of the evolutionary process of exon shuffling in progress. In the case of AtToc75-IV, the region of similarity extends for approximately 50% ...
X-ray Crystallographic Structure of Ibuprofen Bound to Human
... The fatty acid-binding proteins (FABPs) are a family of nine structurally related proteins, which bind to long-chain fatty acids with high affinity. Fatty acid binding proteins were first discovered in 1972, while conducting studies on the factors that regulate the uptake of intestinal fatty acids i ...
... The fatty acid-binding proteins (FABPs) are a family of nine structurally related proteins, which bind to long-chain fatty acids with high affinity. Fatty acid binding proteins were first discovered in 1972, while conducting studies on the factors that regulate the uptake of intestinal fatty acids i ...
pdf
... infect bacteria. The bacteriophage λ can choose between these two “lifestyles.” The molecular basis for this decision is one of the best understood genetic switches that has been studied, and it provides a fundamental paradigm for such molecular switches in developmental biology. This chapter review ...
... infect bacteria. The bacteriophage λ can choose between these two “lifestyles.” The molecular basis for this decision is one of the best understood genetic switches that has been studied, and it provides a fundamental paradigm for such molecular switches in developmental biology. This chapter review ...
