Molecular Characterization of CDC42, a Saccharomyces cerevisiae
... CDC42 revealed a high degree of similarity in amino acid sequence to the ras and rho (Madaule, P., R. Axel, and A. M. Myers. 1987. Proc. Natl. Acad. Sci. 84:779-783) families of gene products. The similarities to ras proteins (,,~40% identical or related amino acids overall) were most pronounced in ...
... CDC42 revealed a high degree of similarity in amino acid sequence to the ras and rho (Madaule, P., R. Axel, and A. M. Myers. 1987. Proc. Natl. Acad. Sci. 84:779-783) families of gene products. The similarities to ras proteins (,,~40% identical or related amino acids overall) were most pronounced in ...
Molecular and Structural Characterization of
... et al., 2000, 2001; Zouhar et al., 2001) and are thought to be essential for the recognition of the aglycone moiety. Furthermore, Ala-467 was demonstrated to make contact with the methoxy group of DIMBOAGlc. While Trp-378 and Phe-198 are conserved in rye at the corresponding positions, Phe-205, Phe- ...
... et al., 2000, 2001; Zouhar et al., 2001) and are thought to be essential for the recognition of the aglycone moiety. Furthermore, Ala-467 was demonstrated to make contact with the methoxy group of DIMBOAGlc. While Trp-378 and Phe-198 are conserved in rye at the corresponding positions, Phe-205, Phe- ...
Insulin-like growth factor binding protein-2 (IGFBP
... pairs of primers (IGFBP-2a-f, IGFBP-2a-r and IGFBP2b-f, IGFBP-2b-r) were designed in non-conservative region for cloning and verifying the partial fragment of P. olivaceus IGFBP-2. In PCR amplification, 1 lL cDNA template, 2 lL of 109 Ex Taq buffer, 1.6 lL of dNTP (2.5 mM of each), 1 lL of the forwa ...
... pairs of primers (IGFBP-2a-f, IGFBP-2a-r and IGFBP2b-f, IGFBP-2b-r) were designed in non-conservative region for cloning and verifying the partial fragment of P. olivaceus IGFBP-2. In PCR amplification, 1 lL cDNA template, 2 lL of 109 Ex Taq buffer, 1.6 lL of dNTP (2.5 mM of each), 1 lL of the forwa ...
Pierce BCA Protein Assay Kit
... Exercise care when re-using glassware. All glassware must be cleaned and given a thorough final rinse with ultrapure water. D. Response characteristics for different proteins Each of the commonly used total protein assay methods exhibits some degree of varying response toward different proteins. The ...
... Exercise care when re-using glassware. All glassware must be cleaned and given a thorough final rinse with ultrapure water. D. Response characteristics for different proteins Each of the commonly used total protein assay methods exhibits some degree of varying response toward different proteins. The ...
The phosphorylation of proteins: a major mechanism for biological
... that is regulated not on1 by pliosphorylation~depliosphorylation but also by Cax ions. Then there are two types of myosin light chain kinase (MLCK), an enzyme first discovered by Pircs et al. (1974). Finally, there is a sonicwhat less specific Ca2+ (calmodu1in)-dependent protein kinase, which is des ...
... that is regulated not on1 by pliosphorylation~depliosphorylation but also by Cax ions. Then there are two types of myosin light chain kinase (MLCK), an enzyme first discovered by Pircs et al. (1974). Finally, there is a sonicwhat less specific Ca2+ (calmodu1in)-dependent protein kinase, which is des ...
Ben-Hur1 pdf
... classification. Leslie and co-authors have focused on various flavors of kernels that represent sequences in the space of k-mers, allowing gaps and mismatches; these include the spectrum and mismatch kernels (Leslie et al., 2002a,b). The k-mers used by these methods are analogous to the discrete mot ...
... classification. Leslie and co-authors have focused on various flavors of kernels that represent sequences in the space of k-mers, allowing gaps and mismatches; these include the spectrum and mismatch kernels (Leslie et al., 2002a,b). The k-mers used by these methods are analogous to the discrete mot ...
Full Text - Journal of Pharmaceutical, Chemical and
... Ginkgo biloba leaves mainly contain these two physiological active ingredients: flavonoids and terpene lactones. Flavonoids have an important role in regulating plant growth and development. Anthocyanidin reductase is a key enzyme involved in the flavonoids biosynthesis. Based on the EST sequence, t ...
... Ginkgo biloba leaves mainly contain these two physiological active ingredients: flavonoids and terpene lactones. Flavonoids have an important role in regulating plant growth and development. Anthocyanidin reductase is a key enzyme involved in the flavonoids biosynthesis. Based on the EST sequence, t ...
Model key paper ppt presentation
... “Description” (especially big ugly titles that take up an entire slide or a large portion of one) Introduce each experiment with the specific question it seeks to answer It is generally obvious what will be procedures, results etc. ...
... “Description” (especially big ugly titles that take up an entire slide or a large portion of one) Introduce each experiment with the specific question it seeks to answer It is generally obvious what will be procedures, results etc. ...
Des - Evolution of Developmental Genes
... • Cloning of opossum IGF2 was succesful • 3 of 4 sequences were identical to each other and to opossum IGF-2 ...
... • Cloning of opossum IGF2 was succesful • 3 of 4 sequences were identical to each other and to opossum IGF-2 ...
Number 53, 2006 5 m sh4
... from the Dmsh4 mutants contain more bubble asci (Perkins and Barry, 1977) and fewer normal size asci than the control. Asci containing less than eight spores or at least one misshapen spore are common in Dmsh4 crosses, regardless of whether the mutant is the male or female parent, but rarely observe ...
... from the Dmsh4 mutants contain more bubble asci (Perkins and Barry, 1977) and fewer normal size asci than the control. Asci containing less than eight spores or at least one misshapen spore are common in Dmsh4 crosses, regardless of whether the mutant is the male or female parent, but rarely observe ...
Metabolic rate depression in animals
... expenditure by ATP-dependent ion pumps must also be reduced and, to maintain homeostasis, the opposing facilitated diffusion of ions through ion channels must also be suppressed. Channel arrest predicts a coordinated suppression of membrane channels and pumps in both plasma and organelle membranes an ...
... expenditure by ATP-dependent ion pumps must also be reduced and, to maintain homeostasis, the opposing facilitated diffusion of ions through ion channels must also be suppressed. Channel arrest predicts a coordinated suppression of membrane channels and pumps in both plasma and organelle membranes an ...
... fragments, analogs and derivatives thereof. the art, for detecting the presence of a specific nucleic acid In accordance with another aspect of the present sequence in a sample obtained from cells, such as from invention, there are provided isolated nucleic acid molecules blood, urine, saliva, tissu ...
Inositol 1,3,4,5,6-Pentakisphosphate 2-Kinase
... (Fig. 3). The genomic ZmIPK1A sequence contains only one EcoRI site (Fig. 2) in the predicted promoter region of the gene. If the ZmIPK1 gene is present in a single copy in the genome, hybridization with a 32P-labeled cDNA probe (as shown in Fig. 2) should detect only one band larger than 9 kb. As s ...
... (Fig. 3). The genomic ZmIPK1A sequence contains only one EcoRI site (Fig. 2) in the predicted promoter region of the gene. If the ZmIPK1 gene is present in a single copy in the genome, hybridization with a 32P-labeled cDNA probe (as shown in Fig. 2) should detect only one band larger than 9 kb. As s ...
Full-Text PDF
... viruses including poxviruses [26,27], and to RNA viruses including coronaviruses and flaviviruses, through the development of infectious cDNA clones [28,29]. 3. Advantages of Bacterial Artificial Chromosomes The cloning of large fragments of genomic DNA for the past three decades has underpinned adv ...
... viruses including poxviruses [26,27], and to RNA viruses including coronaviruses and flaviviruses, through the development of infectious cDNA clones [28,29]. 3. Advantages of Bacterial Artificial Chromosomes The cloning of large fragments of genomic DNA for the past three decades has underpinned adv ...
Mitochondrial stress causes increased succination of proteins in
... We have previously described 2SC [S-(2-succino)-cysteine], a natural chemical modification of proteins [1,2], formed by a Michael addition reaction of the tricarboxylic acid cycle metabolite, fumarate, with low pK a cysteine residues on proteins [1,2]. Succination of protein, which produces an acid- ...
... We have previously described 2SC [S-(2-succino)-cysteine], a natural chemical modification of proteins [1,2], formed by a Michael addition reaction of the tricarboxylic acid cycle metabolite, fumarate, with low pK a cysteine residues on proteins [1,2]. Succination of protein, which produces an acid- ...
Protein Detection Methods in Proteomics Research
... because the proteomics approach measures quantitative changes in expression levels in biological samples. A number of very different techniques exist. Ideally the detection limit should be as low as possible with an optimal signal to noise ratio. For proper quantification of proteins in typical prote ...
... because the proteomics approach measures quantitative changes in expression levels in biological samples. A number of very different techniques exist. Ideally the detection limit should be as low as possible with an optimal signal to noise ratio. For proper quantification of proteins in typical prote ...
Complete DNA sequence of yeast chromosome II.
... partially overlapping cosmid clones of a purpose-built genomic library from S.cerevisiae strain aS288C, supplemented by an overlapping plasmid clone containing the right telomere. By cross-reference with an ordered library from strain C836, established prior to this work (Stucka, 1992), and by chrom ...
... partially overlapping cosmid clones of a purpose-built genomic library from S.cerevisiae strain aS288C, supplemented by an overlapping plasmid clone containing the right telomere. By cross-reference with an ordered library from strain C836, established prior to this work (Stucka, 1992), and by chrom ...
Molecular Cloning, Sequencing, and Expression of the Glutamine
... providing glutamine for biosynthesis and in assimilating ammonia. Escherichia coli GS is regulated at the transcriptional level by the Ntr (nitrogen regulation) system and at the posttranslational level through an adenylylation cascade in which each of 12 identical subunits is reversibly adenylylate ...
... providing glutamine for biosynthesis and in assimilating ammonia. Escherichia coli GS is regulated at the transcriptional level by the Ntr (nitrogen regulation) system and at the posttranslational level through an adenylylation cascade in which each of 12 identical subunits is reversibly adenylylate ...
Type and timing of protein feeding to optimize anabolism
... protein degradation and for protein synthesis. In humans, it is likely that the inhibition of whole body protein degradation occurs in splanchnic organs, as an amino acid infusion that resulted in a decrease in whole body protein breakdown did not affect leg muscle protein breakdown [11]. The higher ...
... protein degradation and for protein synthesis. In humans, it is likely that the inhibition of whole body protein degradation occurs in splanchnic organs, as an amino acid infusion that resulted in a decrease in whole body protein breakdown did not affect leg muscle protein breakdown [11]. The higher ...
Fluorescent Reporter Proteins for the Tonoplast
... species and cell types and are therefore used as specific markers for different vacuolar types, with a-TIP identifying PSVs and g-TIP labeling LVs (Jauh et al., 1998, 1999). This model for vacuolar biogenesis and transport therefore arises from the amalgamation of a large number of biochemical and m ...
... species and cell types and are therefore used as specific markers for different vacuolar types, with a-TIP identifying PSVs and g-TIP labeling LVs (Jauh et al., 1998, 1999). This model for vacuolar biogenesis and transport therefore arises from the amalgamation of a large number of biochemical and m ...
Effect of RNAi down-regulation of three lysine-deficient
... transgene confirmation region. (C) The last probe used was specific for pmi. The last 11 samples (C, D and E – right panel) were analyzed on a second membrane to confirm the complete transgene integration. The same order of probe binding was employed, i.e. (D) for 5’ promoter region, (E) for the con ...
... transgene confirmation region. (C) The last probe used was specific for pmi. The last 11 samples (C, D and E – right panel) were analyzed on a second membrane to confirm the complete transgene integration. The same order of probe binding was employed, i.e. (D) for 5’ promoter region, (E) for the con ...
34750 - Radboud Repository
... similar functional bias toward energy conversion, amino acid metabolism, and protein synthesis is also found in modern mitochondria. However, here the bias appears stronger, specifically toward energy conversion and toward protein synthesis and folding that together represent more than the 50% of the ...
... similar functional bias toward energy conversion, amino acid metabolism, and protein synthesis is also found in modern mitochondria. However, here the bias appears stronger, specifically toward energy conversion and toward protein synthesis and folding that together represent more than the 50% of the ...
New concepts of endoplasmic reticulum function in the heart
... can be survival-, or death-oriented [21]. 3. The ER stress response in the heart Interest in the ER stress response in the heart was spawned partly by the realization that myocardial ischemia and cardiac hypertrophy might alter the ER in cardiac myocytes in ways that would be predicted to impair pro ...
... can be survival-, or death-oriented [21]. 3. The ER stress response in the heart Interest in the ER stress response in the heart was spawned partly by the realization that myocardial ischemia and cardiac hypertrophy might alter the ER in cardiac myocytes in ways that would be predicted to impair pro ...
BCA Assay
... alkaline medium (the biuret reaction) with the highly sensitive and selective colorimetric detection of the cuprous cation (Cu+1) using a unique reagent containing bicinchoninic acid.1 The purple-colored reaction product of this assay is formed by the chelation of two molecules of BCA with one cupro ...
... alkaline medium (the biuret reaction) with the highly sensitive and selective colorimetric detection of the cuprous cation (Cu+1) using a unique reagent containing bicinchoninic acid.1 The purple-colored reaction product of this assay is formed by the chelation of two molecules of BCA with one cupro ...
Continued..
... Motif can refer to a set of contiguous secondary structure elements that either have a particular functional significance or define a portion of an independently folded domain. The elements with the functional sequence motifs are known as functional motifs. An example is the helix-turn-helix mot ...
... Motif can refer to a set of contiguous secondary structure elements that either have a particular functional significance or define a portion of an independently folded domain. The elements with the functional sequence motifs are known as functional motifs. An example is the helix-turn-helix mot ...