Protein thermostability in Archaea and Eubacteria
... In addition to a preference for certain amino acids, there may be differences in their distribution, which may provide different structural adaptations that affect thermostability (Jaenicke, 1996; Gianese et al., 2002). Vieille et al. (2001) believe that the conserved core in mesophiles and thermoph ...
... In addition to a preference for certain amino acids, there may be differences in their distribution, which may provide different structural adaptations that affect thermostability (Jaenicke, 1996; Gianese et al., 2002). Vieille et al. (2001) believe that the conserved core in mesophiles and thermoph ...
13059_2010_2366_MOESM1_ESM
... limited number of enzymes dedicated to chitin metabolism, its genome contains a single family GT2 chitin synthase and three GH18 enzymes distantly related to chitinases, while no GH20 candidate b-N-acetylglucosaminidase or any family CBM18 chitin-binding modules are identified. The chitin synthase g ...
... limited number of enzymes dedicated to chitin metabolism, its genome contains a single family GT2 chitin synthase and three GH18 enzymes distantly related to chitinases, while no GH20 candidate b-N-acetylglucosaminidase or any family CBM18 chitin-binding modules are identified. The chitin synthase g ...
SALT BRIDGE D526- FUNCTIONS AS A HINGE THAT CONTROLS
... single site binding model (Fig. 3A). From the linear plots of kobs vs. protein concentration, k-1 (y-intercept) and k+1 (slope) were obtained (Fig. 3B). These parameters are clearly different for the mutants analyzed here, as seen in Table 1. While the kinetic constants of wt and DnaK3A are very sim ...
... single site binding model (Fig. 3A). From the linear plots of kobs vs. protein concentration, k-1 (y-intercept) and k+1 (slope) were obtained (Fig. 3B). These parameters are clearly different for the mutants analyzed here, as seen in Table 1. While the kinetic constants of wt and DnaK3A are very sim ...
A tale of two functions: enzymatic activity and
... Detection of CT–RNA interaction in situ Detection of CT binding to RNA in situ was accomplished using a fluorescence resonance energy transfer (FRET) assay with a CT b-enhanced yellow fluorescent protein (eYFP) fusion protein as the donor and the RNA binding dye Sytox Orange (Molecular Probes) as the ...
... Detection of CT–RNA interaction in situ Detection of CT binding to RNA in situ was accomplished using a fluorescence resonance energy transfer (FRET) assay with a CT b-enhanced yellow fluorescent protein (eYFP) fusion protein as the donor and the RNA binding dye Sytox Orange (Molecular Probes) as the ...
... answer using either glucose storage/release from glycogen, as regulated by hormones, or glycolysis or the TCA cycle, as regulated by energy sensing. Protein phosphorylation: Enzyme in pathways can be substrates for kinases. The phosphorylated form of the protein can be active or inactive. Dephosphor ...
Protein Measurement with the Folin Phenol Reagent
... It is unnecessary to bring all the samples and standards to the same volume before the addition of the alkaline copper reagent, provided corThe critical volrections are made for small differences in final volume. umes are those of the alkaline copper and Folin reagents. If the protein is present in ...
... It is unnecessary to bring all the samples and standards to the same volume before the addition of the alkaline copper reagent, provided corThe critical volrections are made for small differences in final volume. umes are those of the alkaline copper and Folin reagents. If the protein is present in ...
Possible Roles of Plant Sulfurtransferases in Detoxification of
... superfamily and share the Cdc25 active site motif His-Cys-(X)5-Arg. They do not exhibit significant ...
... superfamily and share the Cdc25 active site motif His-Cys-(X)5-Arg. They do not exhibit significant ...
Gene Section CDK4 (cyclin-dependent kinase 4) Atlas of Genetics and Cytogenetics
... Hayward NK, Akslen LA. A large Norwegian family with inherited malignant melanoma, multiple atypical nevi, and CDK4 mutation. Genes Chromosomes Cancer 2005;44:10-18. This article should be referenced as such: Molven A. CDK4 (cyclin-dependent kinase 4). Atlas Genet ...
... Hayward NK, Akslen LA. A large Norwegian family with inherited malignant melanoma, multiple atypical nevi, and CDK4 mutation. Genes Chromosomes Cancer 2005;44:10-18. This article should be referenced as such: Molven A. CDK4 (cyclin-dependent kinase 4). Atlas Genet ...
Biochimie
... lipid-biosynthesis pathways with molecular tools. Moreover, these strategies can be applied to algae which produce TAG under conditions of nutrient deficiency or environmental stress, and are amendable to genetic transformation [10]. Another feasible approach is the regulation of gene expression by t ...
... lipid-biosynthesis pathways with molecular tools. Moreover, these strategies can be applied to algae which produce TAG under conditions of nutrient deficiency or environmental stress, and are amendable to genetic transformation [10]. Another feasible approach is the regulation of gene expression by t ...
Structural and functional study of K453E mutant protective
... with the wild-type, or mutant PPCA cDNA having either the K453E or Y249N mutation in an expression vector, pCAGGS (Miyazaki et al. 1989), by the calcium phosphateglycerol shock technique. The cells were harvested after 60h and the enzyme activities were determined. The metabolic study was performed ...
... with the wild-type, or mutant PPCA cDNA having either the K453E or Y249N mutation in an expression vector, pCAGGS (Miyazaki et al. 1989), by the calcium phosphateglycerol shock technique. The cells were harvested after 60h and the enzyme activities were determined. The metabolic study was performed ...
Analysis of expressed sequence tags from Cryptomeria japonica
... was used for reverse sequencing. Clones from which highquality sequences had not been determined because of the presence of long polyA tails were selected for sequencing with a mixture of dT-anchored primers (5′-TTTTTTTTTTTTTTTTTTTTV-3′, where V = A, C and G). Raw sequence-trace files were processed ...
... was used for reverse sequencing. Clones from which highquality sequences had not been determined because of the presence of long polyA tails were selected for sequencing with a mixture of dT-anchored primers (5′-TTTTTTTTTTTTTTTTTTTTV-3′, where V = A, C and G). Raw sequence-trace files were processed ...
Characterisation and functional properties of watermelon (Citrullus
... Watermelon is an important but underutilised crop grown in tropical regions of the world. It is used for the production of juices, nectars, fruit cocktails, etc.1 but generates waste in the form of rind and seeds. The seeds are utilised directly for human consumption in various forms, such as snacks ...
... Watermelon is an important but underutilised crop grown in tropical regions of the world. It is used for the production of juices, nectars, fruit cocktails, etc.1 but generates waste in the form of rind and seeds. The seeds are utilised directly for human consumption in various forms, such as snacks ...
The proteome of Saccharomyces cerevisiae mitochondria
... currently available information, however, only ⬇50–60% of all presumed mitochondrial proteins have been identified so far (8–20). Thus, our knowledge about the physiological functions of mitochondria is limited, and many mitochondrial diseases cannot be analyzed on a molecular level. The Saccharomyc ...
... currently available information, however, only ⬇50–60% of all presumed mitochondrial proteins have been identified so far (8–20). Thus, our knowledge about the physiological functions of mitochondria is limited, and many mitochondrial diseases cannot be analyzed on a molecular level. The Saccharomyc ...
See Source - Pentelute Lab
... peptide KLPETGG. During the initial stages of our studies Chen et al. reported an evolved SrtA (SrtA*) that had improved enzymatic kinetics. 26 Once we confirmed SrtA* and SrtA gave similar product yield in model peptide studies, we chose to work exclusively with SrtA* because it substantially short ...
... peptide KLPETGG. During the initial stages of our studies Chen et al. reported an evolved SrtA (SrtA*) that had improved enzymatic kinetics. 26 Once we confirmed SrtA* and SrtA gave similar product yield in model peptide studies, we chose to work exclusively with SrtA* because it substantially short ...
Protein measurement with the Folin-phenol reagent
... It is unnecessary to bring all the samples and standards to the same volume before the addition of the alkaline copper reagent, provided corThe critical volrections are made for small differences in final volume. umes are those of the alkaline copper and Folin reagents. If the protein is present in ...
... It is unnecessary to bring all the samples and standards to the same volume before the addition of the alkaline copper reagent, provided corThe critical volrections are made for small differences in final volume. umes are those of the alkaline copper and Folin reagents. If the protein is present in ...
Journal of Agricultural and Food Chemistry
... also indicated an important role of soluble glutamine as a nitrogen-transporting compound (12). In this report, only protein glutamine was determined. Glutamine accumulation correlated with the accumulation of cytosolic glutamine synthetase (11). Other researchers suggest that several amino acids, a ...
... also indicated an important role of soluble glutamine as a nitrogen-transporting compound (12). In this report, only protein glutamine was determined. Glutamine accumulation correlated with the accumulation of cytosolic glutamine synthetase (11). Other researchers suggest that several amino acids, a ...
LABORATORY Exercise Protein Structure
... The sequence of Amino Acid Sidechains that you determined when placing them on the minitoober is called the Primary Structure of your protein. As a general rule the final shape of a protein is determined by its primary structure (the sequence of its Amino Acids). 5) Now you can begin to fold your 15 ...
... The sequence of Amino Acid Sidechains that you determined when placing them on the minitoober is called the Primary Structure of your protein. As a general rule the final shape of a protein is determined by its primary structure (the sequence of its Amino Acids). 5) Now you can begin to fold your 15 ...
Folie 1 - FLI
... distortions, loops and side chains, as well as detecting errors in a model. Despite these problems, it is currently possible to model with useful accuracy significant parts of approximately one third of all known protein sequences. ...
... distortions, loops and side chains, as well as detecting errors in a model. Despite these problems, it is currently possible to model with useful accuracy significant parts of approximately one third of all known protein sequences. ...
Understanding the functional difference between growth
... GAS6 and PROS1 probably occurred during the whole-genome duplications in vertebrates. A total of 78 amino acid sites were identified to be under functional divergence. One of these sites, Asn463, is involved in N-glycosylation in GAS6, but is mutated in PROS1, preventing this post-translational modi ...
... GAS6 and PROS1 probably occurred during the whole-genome duplications in vertebrates. A total of 78 amino acid sites were identified to be under functional divergence. One of these sites, Asn463, is involved in N-glycosylation in GAS6, but is mutated in PROS1, preventing this post-translational modi ...
Delivering of Proteins to the Plant Vacuole—An Update
... to the vacuole, where the fusion of the PVC/MVB with the tonoplast occurs [15]. As the trafficking to the vacuole can occur by different routes and may involve different intermediates it will be approached in more detail in the next section. In a simplified version of protein transport to the vacuol ...
... to the vacuole, where the fusion of the PVC/MVB with the tonoplast occurs [15]. As the trafficking to the vacuole can occur by different routes and may involve different intermediates it will be approached in more detail in the next section. In a simplified version of protein transport to the vacuol ...
DOCX - The Human Proteome Project
... intensity patterns. All SRM runs performed must have spiked-in heavy labeled peptides corresponding to the putative identifications. Annotated chromatograms must be provided in the supplementary material for the manuscript. Remember that solid peptide sequence evidence does not alter the uncertainti ...
... intensity patterns. All SRM runs performed must have spiked-in heavy labeled peptides corresponding to the putative identifications. Annotated chromatograms must be provided in the supplementary material for the manuscript. Remember that solid peptide sequence evidence does not alter the uncertainti ...
The mapping of linear B-cell epitope regions in desmoglein 1 and 3
... recombinant protein fragments [29]. Bhol et al. identified two antigenic protein fragments BOS1 (aa50-79) and BOS6 (aa200-229) from Dgs3 protein sequence [18] with the usage of 12 synthetic 30mer peptides nearly covering the Dsg3 aa50-468 region. Dworschak et al. analyzed 254 14mer peptides from the ...
... recombinant protein fragments [29]. Bhol et al. identified two antigenic protein fragments BOS1 (aa50-79) and BOS6 (aa200-229) from Dgs3 protein sequence [18] with the usage of 12 synthetic 30mer peptides nearly covering the Dsg3 aa50-468 region. Dworschak et al. analyzed 254 14mer peptides from the ...
Plant cell walls as targets for biotechnology
... dietary fibers. They are used widely in the food industry as gelling and thickening agents. Along with storage proteins, unique features of the cell walls in different seed flours alter baking properties and crumb textures. Cell walls are the principal textural components of fruits and vegetables, a ...
... dietary fibers. They are used widely in the food industry as gelling and thickening agents. Along with storage proteins, unique features of the cell walls in different seed flours alter baking properties and crumb textures. Cell walls are the principal textural components of fruits and vegetables, a ...
Dehydrogenase in Saccharomyces cerevisiae
... -571), which would be read in the opposite sense, is capable of encoding a polypeptide of 77 residues; the other (sequence not complete, terminating at - 858) could encode a polypeptide of at least 76 amino acids. A search of the NBRF protein data base showed no strong homology between the two predi ...
... -571), which would be read in the opposite sense, is capable of encoding a polypeptide of 77 residues; the other (sequence not complete, terminating at - 858) could encode a polypeptide of at least 76 amino acids. A search of the NBRF protein data base showed no strong homology between the two predi ...
Dehydrogenase in Saccharomyces cerevisiae
... -571), which would be read in the opposite sense, is capable of encoding a polypeptide of 77 residues; the other (sequence not complete, terminating at - 858) could encode a polypeptide of at least 76 amino acids. A search of the NBRF protein data base showed no strong homology between the two predi ...
... -571), which would be read in the opposite sense, is capable of encoding a polypeptide of 77 residues; the other (sequence not complete, terminating at - 858) could encode a polypeptide of at least 76 amino acids. A search of the NBRF protein data base showed no strong homology between the two predi ...