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Improving protein fold recognition with hybrid
Improving protein fold recognition with hybrid

... best approach for protein fold recognition. Besides these methods solely based on sequence, some authors have proposed structure-based fold recognition methods called ‘threading’ (Bowie et al., 1991; Jones et al., 1992). The principle of threading is to assess the compatibility between the target se ...
Possibilities for feeding low-protein diets to weanling and growing pigs
Possibilities for feeding low-protein diets to weanling and growing pigs

... these pigs. Several different Ideal Protein ratios have been suggested, but the ratios most widely used are the ratios proposed in the so-called Illinois Ideal Protein (Baker, 1997). In the Illinois Ideal Protein, three different ratios are suggested depending on the weight of the pig, i.e., from 1 ...
The role of mutations in core protein of hepatitis B
The role of mutations in core protein of hepatitis B

... closely with RNA [5]. In this domain phosphorylated site residues located in amino acid sequences 155-183. Immature nucleocapsids which contain RNA are phosphorylated at six sites, while the mature nucleocapsids which contain DNA are completely dephosphorylated either inside cells or in extracellula ...
Determination of Molecular Weight of
Determination of Molecular Weight of

... In the case of a glycoprotein, a similar calculation can be used to estimate partial specific volume if the carbohydrate content is known, and again good agreement between experimental and calculated values has been shown for glycoproteins (8). Obviously, if the carbohydrate content is unknown, it i ...
Health relevance of intestinal protein fermentation in young
Health relevance of intestinal protein fermentation in young

... Knowledge about the undigested protein fractions and their fermentability throughout the different parts of the intestine is still scarce. Usually, protein in animal feed is determined roughly as N × 6.25 and further characterized by the standardized ileal amino acid digestibility, whereas the relat ...
Novel regulatory roles of cAMP receptor proteins in
Novel regulatory roles of cAMP receptor proteins in

... a result of this activation, the levels of cAMP increase, which in turn binds to CRP, resulting in the formation of the cAMP–CRP binary complex (Saier & Reizer, 1994). The cAMP–CRP complex binds at promoters containing a specific DNA sequence (consensus 59-TGTGAN6TCACA39) to regulate expression of t ...
Protein domains as units of genetic transfer
Protein domains as units of genetic transfer

... Fig. 4. Distances between inferred breakpoint and the nearest protein domain boundary based on divergence of recombining sequences: (A) low, (B) moderate and (C) high divergence. The panels of (i) and (ii) show the observed distances and expected distances, respectively. For each graph in (i) and (i ...
Protein Synthesis, Processing, and Regulation
Protein Synthesis, Processing, and Regulation

... Proteins are the active players in most cell processes, implementing the myriad tasks that are directed by the information encoded in genomic DNA. Protein synthesis is thus the final stage of gene expression. However, the translation of mRNA is only the first step in the formation of a functional pr ...
Predicting Secondary Structures of Proteins
Predicting Secondary Structures of Proteins

... sentence above is not true for all proteins because some proteins need “auxiliary molecules” to fold. The structural features of proteins have been divided into levels. The first level of the protein structure, called the primary structure, refers just to the sequence of amino acids in the protein. ...
Protein aggregation and amyloid fibril formation prediction software
Protein aggregation and amyloid fibril formation prediction software

... a + b proteins, respectively) [36]. The observed packing density for each amino acid residue is defined as the number of amino acid residues in contact with the given residue (two residues are considered to be in contact if any pair of their non-hydrogen atoms is at a distance < 8 Å). Neighbouring r ...
extraction of keratin protein from chicken feather
extraction of keratin protein from chicken feather

... anti-aging cream, shampoo, and conditioner and for medical purposes such as bone replacement and bone graft. Keywords: chicken feather, reducing agents, protein precipitation, analysis INTRODUCTION The present research work is regarding extracting natural keratin protein from chicken feathers by usi ...


... The enzyme exists in two states relaxed (R) or tense (T) with the relaxed being the active form. ...
Protein: Amino Acids
Protein: Amino Acids

... Once the amino group is removed from the protein, the remaining carbon molecules can be used to create energy - 4 Kcal per gram or stored as fat. • Other roles include being converted to other proteins or making neurotransmitters norepinephrine and epinephrine, melanin, fibrin and as precursor to th ...
Involvement of Endogenous Retroviruses in Prion Diseases
Involvement of Endogenous Retroviruses in Prion Diseases

... a certain direct role for ERVs in the pathogenesis of clinical diseases should be proved more, specific ERV expression has been reported and has emerged as a possible etiological factor in complex diseases [36–38]. In the mouse, a number of ERVs are still active, and those with infectious counterpar ...
It Works! Shake™ Product Info Sheet
It Works! Shake™ Product Info Sheet

... Soy-free, dairy-free, and Non-GMO, this plantbased protein makes It Works! Shake a great choice for vegans, vegetarians, or anyone with soy/dairy sensitivity or anyone who wants to ensure their protein is clean! What are the “plants” used in the plant-based protein of It Works! Shake? It Works! Shak ...
Khoa hoc - Cong nghe
Khoa hoc - Cong nghe

... coincides with research of scad hydrolysis by Flavourzyme (Chun et al., 2006). A number of other studies on hydrolyzed tuna head (Nguyen et al., 2012) and hydrolysis salmon head (Sathivel et al., 2005) also showed that essential amino acids content in hydrolyzed protein obtained from these studied w ...
TCU Taiwan Championship Poster
TCU Taiwan Championship Poster

... Filamentous phage can only infect bacteria with F pili. Its life cycle described in following steps: Adsorption: In this step, M13 and M13KO7 are the same. Both of their pilus attachment protein gp3 and gp6 will help them bind on F pilus, then the phage will move into host cell by F pilus and take o ...
all plant protein - frequently asked questions
all plant protein - frequently asked questions

... Adults who are interested in maintaining a strong healthy body will benefit the most from this product. NUTRILITE™ All Plant Protein is a plant-based source of high quality protein that can be used to replace higher fat animal proteins in the diet. 3. What are potential benefits for using this produ ...
Evolutionary Gain of Function for the ER Membrane
Evolutionary Gain of Function for the ER Membrane

... (Goldshmidt et al., 2008; Zimmerman and Blatch, 2009). In mammalian cells, the lumenal Hsp70 immunoglobulin heavy-chain binding protein (BiP) and a so far unidentified Hsp40 are also involved in cotranslational protein import into the organelle (Dierks et al., 1996; Hamman et al., 1998; Tyedmers et ...
Methods of Protein Analysis
Methods of Protein Analysis

... is inversely related to the protein content of the sample. 3. Infrared Reflectance Method. In 1971, in­ struments using infrared reflectance (Neotec and Infraalyzer) were introduced into the grain trade to measure protein, moisture and oil. In this meth­ od, infrared light of different wave lengths ...
Thermodynamic analysis of the unfolding and stability of the dimeric
Thermodynamic analysis of the unfolding and stability of the dimeric

... positively charged clusters. The wild-type protein is more stable than its E34D mutant, particularly at pH 4.00 and 3.75 although less so at 3.50 (1.8, 1.6 and 0.6 kJÆmol)1 at 25 C for DDG at pH 4.00, 3.75 and 3.50, respectively), which seems to be related to the effect of a salt bridge between E34 ...
The Structure and Topology of Protein Serine/Threonine
The Structure and Topology of Protein Serine/Threonine

... [18]. The mechanism of inhibition of PP1 by okadaic acid and microcystin LR have been defined by structures of PP1 in complex with these inhibitors. Both inhibitors, although structurally different, bind to a similar region of the phosphatase, occupying the catalytic channel to directly block phosph ...
Ashley, CT, Wilkinson, KD, Reines, D and Warren, ST: FMR1 protein: Conserved RNP family domains and selective RNA binding. Science 262:563-566 (1993).
Ashley, CT, Wilkinson, KD, Reines, D and Warren, ST: FMR1 protein: Conserved RNP family domains and selective RNA binding. Science 262:563-566 (1993).

... Claude T. Ashley Jr., Keith D. Wilkinson, Daniel Reines, Stephen T. Warren* Fragile X syndrome is the result of transcriptional suppression of the gene FMR1 as a result of a trinucleotide repeat expansion mutation. The normal function of the FMR1 protein (FMRP) and the mechanism by which its absence ...
In-vitro Protein Production for Structure Determination with the Rapid
In-vitro Protein Production for Structure Determination with the Rapid

... genomes of Mycoplasma pneumoniae and Mycoplasma genitalium or structural homologs from other organisms. To achieve this goal, the Berkeley Structural Genomics Center is developing high-throughput methods for protein expression for use in X-ray and NMR structure determination. In collaboration with R ...
Dreze et al, Methods Enzymol 2010
Dreze et al, Methods Enzymol 2010

... transcription factors in their natural context but can behave as autoactivators because they contain a cryptic AD (cognate autoactivators), and (iii) nontranscription factor proteins that contain one or more cryptic ADs that are only functional as truncated fragments and not when expressed in the co ...
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Expression vector

An expression vector, otherwise known as an expression construct, is usually a plasmid or virus designed for protein expression in cells. The vector is used to introduce a specific gene into a target cell, and can commandeer the cell's mechanism for protein synthesis to produce the protein encoded by the gene. Expression vectors are the basic tools in biotechnology for the production of proteins.The plasmid is engineered to contain regulatory sequences that act as enhancer and promoter regions and lead to efficient transcription of the gene carried on the expression vector. The goal of a well-designed expression vector is the production of protein, and this may be achieve by the production of significant amount of stable messenger RNA, which can then be translated into protein. The protein may be expressed constitutively, or induced when necessary using an inducer. Escherichia coli is commonly used as the host for protein expression, other cell types however may also be used. An example of the use of expression vector is the production of insulin which is used for medical treatments of diabetes.
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