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Transcriptional activity and role of plasmids of
Transcriptional activity and role of plasmids of

... Approximately 64% of the Pc34434568 genome was significantly DE (SDE; P-adjusted < 0.1) during growth in 35 BU hop extract, whereas only 23% of the Lb464 genome was SDE during growth in 50 BU hop extract (Table 1). Furthermore, a higher number of highly SDE genes was seen for Pc344 than for Lb464, w ...
Infrared spectroscopy: a tool for protein characterization Chenge Li
Infrared spectroscopy: a tool for protein characterization Chenge Li

... The contractile force of a muscle is generated through the interaction of two proteins, myosin and actin. In muscle cells, molecules of myosin arrange and form structures called thick filaments. Molecules of globular actin, together with two regulatory proteins, troponin and tropomyosin form the th ...
Evolutionary Adaptation to Different Thermal Environments via
Evolutionary Adaptation to Different Thermal Environments via

... activity to vary independently of changes in the amino acids of a protein. Replacements of the amino acids in an enzyme are necessarily constrained because of the limited number of residues that will both fulfill the necessary catalytic function and maintain secondary and tertiary structure. In addi ...
H_355605_Nenova, Bulgaria.fm
H_355605_Nenova, Bulgaria.fm

... produce them when needed. The nutrition value of sunflower protein is determined by the good balance of the essential amino acids contained in it. Each amino acid plays a specific biological role (Ribarova, 1987). Lysine is one of the three most important essential amino acids (the other two are try ...
Co-opting sulphur-carrier proteins from primary metabolic pathways
Co-opting sulphur-carrier proteins from primary metabolic pathways

... BE-7585A cluster. Subsequent genome sequencing uncovered a few genes encoding sulphur-carrier proteins that are probably involved in the biosynthesis of primary metabolites but only one activating enzyme gene in the A. orientalis genome. Further experiments showed that this activating enzyme can ade ...
Supplementary Information 410 475
Supplementary Information 410 475

... Comparison of vertebrate TSG amino acid sequences. (A) Alignment of TSG amino acid sequences for human (hTSG), mouse (mTSG), chick (cTSG), Xenopus (xTSG), and zebrafish (zTSG) with Drosophila TSG (dTSG) amino acid sequences6 and those of two recently reported gene products of the Drosophila genome ( ...
Does a backwardly read protein sequence have a unique native state?
Does a backwardly read protein sequence have a unique native state?

... exist between the retro-protein structure and the structure of the original protein. Naturally occurring proteins are built from L-amino acids, consecutively connected by amide bonds in order to produce a long backbone of amide bonds with amino acid side groups, which are attached to the chiral C° c ...
Metabolic adaptation of Mycobacterium avium subsp
Metabolic adaptation of Mycobacterium avium subsp

... indicate that MTB uses various pathways that mediate oxidative stress resistance and cell wall synthesis to evade the host immune responses and adapt to its intracellular lifestyle in the macrophage (Mukhopadhyay et al., 2012). A better understanding of mycobacterial metabolism in the infected host ...
The Essential Nature of Sphingolipids in Plants as Revealed by the
The Essential Nature of Sphingolipids in Plants as Revealed by the

... Measurement of SPT activity in isolated microsomes from the lcb1D mutant indicated that neither At LCB1 nor At LCB2 alone has significant activity (Figure 3C), consistent with studies from yeast and mammalian cells showing that both subunits are required for SPT activity (Hanada, 2003). These data f ...
PSLDoc: Protein subcellular localization prediction based on
PSLDoc: Protein subcellular localization prediction based on

... Prediction of protein subcellular localization (PSL) is important for genome annotation, protein function prediction, and drug discovery. Many computational approaches for PSL prediction based on protein sequences have been proposed in recent years for Gram-negative bacteria. We present PSLDoc, a me ...
`Hybrid Protein Model`for optimally defining 3D protein structure
`Hybrid Protein Model`for optimally defining 3D protein structure

... distances, consensus or crystallographic approaches (Colloc’h et al., 1993; Frishman and Argos, 1995; Labesse et al., 1997; King and Johnson, 1999). The α-helix and βsheet repetitive structures represent less than 50% of all protein folds. Attempts to classify coils have not yielded completely satis ...
Molecular Microbial Ecology of the Rhizosphere
Molecular Microbial Ecology of the Rhizosphere

... Plant Root Exudate The analysis of the effects of root exudates on expression of extracellular and cytosolic proteins by two-dimensional (2-D) gel electrophoresis revealed 17 and 21 proteins, respectively, as being significantly altered (Kierul, 2012). Most of the differentially expressed proteins w ...
Protein splicing of PRP8 mini-inteins from species of the genus
Protein splicing of PRP8 mini-inteins from species of the genus

... the causal agent of blue mold rot, the most devastating pathogen in harvested fruits. It causes significant loss to the fruit industry and is of concern in fruit products because of its production of the mycotoxin patulin (Pitt 1979; Majerus and Kapp 2002). P. thomii was found to have no PRP8 intein ...
lncRNA in
lncRNA in

... What are lncRNA’s? • Arbitrarily defined as >200bp RNA’s that do not code for protein • Excludes Pseudogenes and Nonsense Mediated Decay products ...
X(0,25) - IFSC-USP
X(0,25) - IFSC-USP

... Gly residues that turn in a way that only they can.These Glys are retained in all members of a fold family(ie. all Bacterial ribosomal proteins). Pro has a constrained conformation. While Pros can be replaced by other amino acids, specific energetic stability may be lost accounting for conservation. ...
Studying the pathogenesis of renal disease in cystinosis
Studying the pathogenesis of renal disease in cystinosis

... • Discovery of cystinosin in 1998 opened a new chapter of diseases caused by defects of lysosomal transport proteins. • New data indicate that the expression of cystinosin is not restricted to the lysosomal membrane. Ongoing studies will identify new functions and regulators of cystinosin activity  ...
Effect of
Effect of

... values for Nile tilapia, Oreochromis niloticus (2.42-5.74 g; Brunty, 1997). Many factors may affect the TAN level including temperature, salinity, fish species, other water parameters, and experimental design. In the ornamental fish industry, water quality is very important to the health and quality ...
Probing the conformational changes of the yeast mitochondrial ADP
Probing the conformational changes of the yeast mitochondrial ADP

... binding site is accessible to the cytoplasm, whereas in the matrix state, which can be locked by bongkrekic acid, the substrate binding site is open to the mitochondrial matrix. Access to the substrate binding site is regulated by salt bridge networks on either side of the central cavity, called the ...
Chloroplasts at work during plant innate immunity
Chloroplasts at work during plant innate immunity

... Dinesh-Kumar, 2010; Bobik and Burch-Smith, 2015). The chloroplast, together with the nucleus, cell membrane, and endoplasmic reticulum (ER), plays a critical role during the establishment of plant immunity against microbial attack and, in this context, the importance of efficient interorganellar sig ...
Presentation Slides - Society of Barley Engineers
Presentation Slides - Society of Barley Engineers

... (c) Galactosylated glycerol teichoic acid in the cell wall and a changed lipid composition of the cytoplasmic membrane of beer spoilage lactic acid bacteria may increase the barrier to hop compounds. ...
Stabilization by GroEL, a Molecular Chaperone, and a Periplasmic
Stabilization by GroEL, a Molecular Chaperone, and a Periplasmic

... The terminal reductase of such respiration, DMSO reductase (DMSOR), is a soluble periplasmic protein consisting of a single polypeptide and containing one molecule of a molybdenum (Mo) cofactor per molecule (Satoh et al. 1987). DMSOR is synthesized as a precursor, with a molecular mass of 89,206 Da. ...
as a PDF
as a PDF

... previously described above. Fd was further purified by gel filtration on a pre-packed Superdex 75 HR 10/30 column coupled to a HiLoad 26/10 ‘‘Fast flow column’’, both from Amersham Biosciences and previously equilibrated with 50 mM Phosphate buffer (pH 7.6) containing 150 mM NaCl. Fd fractions were ...
An ATPase domain common to prokaryotic cell cycle proteins, sugar
An ATPase domain common to prokaryotic cell cycle proteins, sugar

... adenylate kinase, recA protein, elongation factor Tu, or ras oncogene protein p21 (8). These three structures are not only similar in threedimensional fold but probably also in some aspects of mechanism: their ATPase active sites are lined with identical or similar residues and the overall domain st ...
Rapid increase of cytosolic content of acetyl-CoA
Rapid increase of cytosolic content of acetyl-CoA

... Acetyl-CoA Carboxylase (ACC) catalyzes formation of malonyl-CoA from acetyl-CoA and CO2 which is the ratelimiting step in fatty acid biosynthesis (Wakil et al., 1983; Numa and Tanabe, 1984). Malonyl-CoA serves as a precursor of fatty acid biosynthesis and an intermediate of fatty acid elongation, bu ...
Chlamydia trachomatis RNA polymerase major sigma subunit
Chlamydia trachomatis RNA polymerase major sigma subunit

... 70,000, and 55,000, respectively) (Fig. 1, A and B). Note that the B subunit is an extremely acidic protein; consequently, aberrant migration in the SDS-PAGE system overestimates the actual molecular weight calculated from the nucleotide sequence (10, 13). The 2GlO monoclonal binding data prompted o ...
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Expression vector

An expression vector, otherwise known as an expression construct, is usually a plasmid or virus designed for protein expression in cells. The vector is used to introduce a specific gene into a target cell, and can commandeer the cell's mechanism for protein synthesis to produce the protein encoded by the gene. Expression vectors are the basic tools in biotechnology for the production of proteins.The plasmid is engineered to contain regulatory sequences that act as enhancer and promoter regions and lead to efficient transcription of the gene carried on the expression vector. The goal of a well-designed expression vector is the production of protein, and this may be achieve by the production of significant amount of stable messenger RNA, which can then be translated into protein. The protein may be expressed constitutively, or induced when necessary using an inducer. Escherichia coli is commonly used as the host for protein expression, other cell types however may also be used. An example of the use of expression vector is the production of insulin which is used for medical treatments of diabetes.
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