Osmoadaptative Strategy and Its Molecular
... Halophilic microbes living in hypersaline environments must counteract the detrimental effects of low water activity and salt interference. Some halophilic prokaryotes equilibrate their intracellular osmotic strength with the extracellular milieu by importing inorganic solutes, mainly potassium. The ...
... Halophilic microbes living in hypersaline environments must counteract the detrimental effects of low water activity and salt interference. Some halophilic prokaryotes equilibrate their intracellular osmotic strength with the extracellular milieu by importing inorganic solutes, mainly potassium. The ...
Reduced amino acid alphabets exhibit an improved sensitivity and
... folds in nature is estimated to be between 1000 and 10 000 in total (Grant et al., 2004), an astonishingly low number compared with the huge space of possible amino acid sequences. From the wealth ∗ To ...
... folds in nature is estimated to be between 1000 and 10 000 in total (Grant et al., 2004), an astonishingly low number compared with the huge space of possible amino acid sequences. From the wealth ∗ To ...
Nutritional Control of Growth and Development in Yeast
... of glucose- to glycerol-grown cells can be recapitulated ...
... of glucose- to glycerol-grown cells can be recapitulated ...
Selection of Functional Signal Peptide Cleavage Sites from a Library of Random Sequences.
... reduced. However, the possibility that residues in the N terminus of the signal peptide or the N terminus of the mature ,B-lactamase influence the types of cleavage site sequences selected cannot be eliminated. To select for signal peptide cleavage site sequences that function efficiently, plasmid D ...
... reduced. However, the possibility that residues in the N terminus of the signal peptide or the N terminus of the mature ,B-lactamase influence the types of cleavage site sequences selected cannot be eliminated. To select for signal peptide cleavage site sequences that function efficiently, plasmid D ...
eg1
... multiple forms (Knowles et al. 1987, Kubicek 1992). V. volvacea produces a cellulolytic system that includes multiple forms of all three classes when grown on crystalline cellulose (Cai et al. 1994, 1999). In addition to EG1, four other CMC-hydrolysing proteins were separated in lower yields from cu ...
... multiple forms (Knowles et al. 1987, Kubicek 1992). V. volvacea produces a cellulolytic system that includes multiple forms of all three classes when grown on crystalline cellulose (Cai et al. 1994, 1999). In addition to EG1, four other CMC-hydrolysing proteins were separated in lower yields from cu ...
Olanzapine Activates Hepatic Mammalian Target of Rapamycin
... OLZ-induced weight gain is not only an issue for patient compliance, but can also induce sequelae associated with weight gain/obesity such as glucose intolerance and/or insulin resistance. Interestingly, the changes induced by OLZ administration in carbohydrate and lipid metabolism may in fact prece ...
... OLZ-induced weight gain is not only an issue for patient compliance, but can also induce sequelae associated with weight gain/obesity such as glucose intolerance and/or insulin resistance. Interestingly, the changes induced by OLZ administration in carbohydrate and lipid metabolism may in fact prece ...
Cellular Pathophysiology of Insulin Resistance
... Poretsky LO, ed. New York, Springer,2009, p. 75-87. ...
... Poretsky LO, ed. New York, Springer,2009, p. 75-87. ...
Expression of phosphofructokinase in Neisseria meningitidis
... during the evolution of the species this activity was lost (Bapteste et al., 2003), the question of why N. meningitidis lost PFK activity remains. In particular, since glucose is present within nasopharyngeal tissue (Exley et al., 2005), the presence of PFK can yield one additional ATP per unit of s ...
... during the evolution of the species this activity was lost (Bapteste et al., 2003), the question of why N. meningitidis lost PFK activity remains. In particular, since glucose is present within nasopharyngeal tissue (Exley et al., 2005), the presence of PFK can yield one additional ATP per unit of s ...
Logistic regression models to predict solvent accessible residues
... the accompanying learning and validation sets allow users to explore the utility of residue solvent accessibility (RSA) prediction with simple, physically intuitive models in any number of related applications. Moreover, the corresponding R and Python code can be implemented or otherwise modified to ...
... the accompanying learning and validation sets allow users to explore the utility of residue solvent accessibility (RSA) prediction with simple, physically intuitive models in any number of related applications. Moreover, the corresponding R and Python code can be implemented or otherwise modified to ...
PDF
... of maize has 1782 bp (Supplemental Fig. S1). NtMTHFR1 and NtMTHFR2 from W38 were found to differ by 4% at the nucleotide level. NtMTHFR1 is highly homologous to NsMTHFR1 with only two base pair differences, whereas NtMTHFR2 and NtoMTHFR1 are identical, suggesting that NtMTHFR1 was inherited from N. ...
... of maize has 1782 bp (Supplemental Fig. S1). NtMTHFR1 and NtMTHFR2 from W38 were found to differ by 4% at the nucleotide level. NtMTHFR1 is highly homologous to NsMTHFR1 with only two base pair differences, whereas NtMTHFR2 and NtoMTHFR1 are identical, suggesting that NtMTHFR1 was inherited from N. ...
Oxidized Amino Acids in Lens Protein with Age
... constructed using 0-2.5 ng of DT. The DTcontent of the protein was pmol of DT/mol of Tyr in the oxidized model proteins. For normalized to the content of the precursor amino acid Tyr, determined by amino acid analysis of the hydrolysate (9). As described this reason, a SIM-GC/MS assay was developedf ...
... constructed using 0-2.5 ng of DT. The DTcontent of the protein was pmol of DT/mol of Tyr in the oxidized model proteins. For normalized to the content of the precursor amino acid Tyr, determined by amino acid analysis of the hydrolysate (9). As described this reason, a SIM-GC/MS assay was developedf ...
Conservation and relative importance of residues across protein
... consequently have different evolutionary pressure for their conservation. The degree of conservation of each interface residue can be defined in terms of sequence entropy at that position in the polypeptide chain across all of the homologous proteins. At least three types of contacts can be identifi ...
... consequently have different evolutionary pressure for their conservation. The degree of conservation of each interface residue can be defined in terms of sequence entropy at that position in the polypeptide chain across all of the homologous proteins. At least three types of contacts can be identifi ...
Nutrient Metabolism
... ABSTRACT A restricted protein diet supplemented with amino acids and fat may reduce the acidogenic effects of exercise. Twelve Arabian horses were assigned to a 2 ⫻ 2 factorial experiment: two fat levels: 0 or 10 g/100 g added corn oil and two crude protein levels: 7.5 g/100 g (supplemented with 0.5 ...
... ABSTRACT A restricted protein diet supplemented with amino acids and fat may reduce the acidogenic effects of exercise. Twelve Arabian horses were assigned to a 2 ⫻ 2 factorial experiment: two fat levels: 0 or 10 g/100 g added corn oil and two crude protein levels: 7.5 g/100 g (supplemented with 0.5 ...
Creating Multiple Sequence Alignments
... o Set width of the sliding window: (right click on the Dotter window and select ‘Change size of sliding window’). The default width of 25 residues over which the pairwise scores are averaged has proven to be very robust, but you can change the width of the sliding window. o Print to a file: (right ...
... o Set width of the sliding window: (right click on the Dotter window and select ‘Change size of sliding window’). The default width of 25 residues over which the pairwise scores are averaged has proven to be very robust, but you can change the width of the sliding window. o Print to a file: (right ...
STRUCTURAL AND FUNCTIONAL STUDIES OF PYRIDOXINE 5’-PHOSPHATE SYNTHASE E. COLI Doctoral Thesis
... Pyridoxal 5’-phosphate is the biocatalytically active form of vitamin B6, being one of nature’s most versatile cofactors that plays a central role in the metabolism of amino acids. Whereas microorganisms and plants can synthetise vitamin B6 de novo, mammals have to obtain one of the B6 vitamers with ...
... Pyridoxal 5’-phosphate is the biocatalytically active form of vitamin B6, being one of nature’s most versatile cofactors that plays a central role in the metabolism of amino acids. Whereas microorganisms and plants can synthetise vitamin B6 de novo, mammals have to obtain one of the B6 vitamers with ...
The 92-kDa chitinase from Streptomyces olivaceoviridis contains a
... kDa (Breves, unpublished) was characterized in our laboratory. We isolated 42-, 22- and 14-kDa proteinases by FPLC from the protein concentrate in the presence of Pefabloc SC. The proteins were blotted and the N-terminal amino acid sequence determined. As can be seen from Table 1, the amino acid seq ...
... kDa (Breves, unpublished) was characterized in our laboratory. We isolated 42-, 22- and 14-kDa proteinases by FPLC from the protein concentrate in the presence of Pefabloc SC. The proteins were blotted and the N-terminal amino acid sequence determined. As can be seen from Table 1, the amino acid seq ...
Molecular Cloning and Nucleotide Sequence of the 3
... The 3-IMDH gene of C. utilis was cloned and its nucleotide sequence was determined. This is the first report of the nucleotide sequence of a functional gene of C. utilis as far as we know. The sequence of 2209 bp was an alignment of four restriction fragments determined separately. The sequences of ...
... The 3-IMDH gene of C. utilis was cloned and its nucleotide sequence was determined. This is the first report of the nucleotide sequence of a functional gene of C. utilis as far as we know. The sequence of 2209 bp was an alignment of four restriction fragments determined separately. The sequences of ...
Pol Is a Candidate for the Mouse Pulmonary
... ACGC-3⬘ (BALB/cJ), and reverse, 5⬘-ATAAGGATATCAATCAGGGGAGGCTo examine the 2147 base coding region of the human POL gene for 3⬘; and P2 set: forward, 5⬘-CCTCCCCTGATTGATATCCTTATG-3⬘, and polymorphisms or mutations, we made cDNA from the RNA and amplified reverse, 5⬘-CTCCCTCCATCGATGGACTTATCTGTGCGCCGA ...
... ACGC-3⬘ (BALB/cJ), and reverse, 5⬘-ATAAGGATATCAATCAGGGGAGGCTo examine the 2147 base coding region of the human POL gene for 3⬘; and P2 set: forward, 5⬘-CCTCCCCTGATTGATATCCTTATG-3⬘, and polymorphisms or mutations, we made cDNA from the RNA and amplified reverse, 5⬘-CTCCCTCCATCGATGGACTTATCTGTGCGCCGA ...
Effect of aluminium on plant growth and metabolism
... 5.57, and Al(OH)4 at pH 78. Other complex ions AlO4Al12(OH)24(H2O)127+ (Al13) and Al3+ are almost certainly toxic, but no rhizotoxicity has been detected for AlSO4+ and Al(SO4)2 or Al-F (e.g. AlF2+ and AlF2+). The status of Al(OH)2+ and Al(OH)2+ is uncertain although experimental results have ap ...
... 5.57, and Al(OH)4 at pH 78. Other complex ions AlO4Al12(OH)24(H2O)127+ (Al13) and Al3+ are almost certainly toxic, but no rhizotoxicity has been detected for AlSO4+ and Al(SO4)2 or Al-F (e.g. AlF2+ and AlF2+). The status of Al(OH)2+ and Al(OH)2+ is uncertain although experimental results have ap ...
P.abyssi PDF version
... mutants was successfully used for transformation experiments with the gene pyrE from Sulfolobus solfataricus as a genetic marker inserted in a pGT5 derived shuttle vector (Lucas et al., 2003). Pyrococcus abyssi and closely related strain devoid of plasmids (e.g. GE9) appear thus promising species to ...
... mutants was successfully used for transformation experiments with the gene pyrE from Sulfolobus solfataricus as a genetic marker inserted in a pGT5 derived shuttle vector (Lucas et al., 2003). Pyrococcus abyssi and closely related strain devoid of plasmids (e.g. GE9) appear thus promising species to ...
proteins
... ■ While some proteins do not require any more assembly after the quaternary level, other proteins require additional processing to reach their functional stage. – Many proteins are not ready to be functional until they’ve undergone posttranslational processing. – Posttranslational processing refers ...
... ■ While some proteins do not require any more assembly after the quaternary level, other proteins require additional processing to reach their functional stage. – Many proteins are not ready to be functional until they’ve undergone posttranslational processing. – Posttranslational processing refers ...
Although the administration of testosterone clearly causes marked
... 6) containing 0.4 per cent 2,5-diphenyloxazole and 0.02 per cent 1,4-bis-2 (5-phenyloxazolyl) -benzene and assayed for C1' in a Packard Tri-Carb liquid scintillation counter as described by Bray (14). In some experiments free tyrosine was determined on another portion of the supernatant by the spect ...
... 6) containing 0.4 per cent 2,5-diphenyloxazole and 0.02 per cent 1,4-bis-2 (5-phenyloxazolyl) -benzene and assayed for C1' in a Packard Tri-Carb liquid scintillation counter as described by Bray (14). In some experiments free tyrosine was determined on another portion of the supernatant by the spect ...
Article Fitness Trade-Offs Determine the Role of the Molecular
... drift imposed by frequent single-colony bottlenecks, and 2) populations evolving under mild genetic drift imposed by serial transfers (fig. 1). All populations were initiated from a single hypermutable clone of E. coli that lacks the DNA mismatch repair gene mutS (mutS) and has a mutation rate 3 or ...
... drift imposed by frequent single-colony bottlenecks, and 2) populations evolving under mild genetic drift imposed by serial transfers (fig. 1). All populations were initiated from a single hypermutable clone of E. coli that lacks the DNA mismatch repair gene mutS (mutS) and has a mutation rate 3 or ...
Hypothesis: Hypoxia induces de novo synthesis of NeuGc
... of them was the GM3 synthase (ST3GAL5/SLC35E1) responsible for catalyzing the covalent addition of sialic acid to lactosylceramide to generate the GM3 ganglioside. We found, using SILAC, that GM3 synthase was up-regulated 1.5 fold (although with low statistical significance; p=0.11), which was confi ...
... of them was the GM3 synthase (ST3GAL5/SLC35E1) responsible for catalyzing the covalent addition of sialic acid to lactosylceramide to generate the GM3 ganglioside. We found, using SILAC, that GM3 synthase was up-regulated 1.5 fold (although with low statistical significance; p=0.11), which was confi ...
N-Terminal Intramolecularly Conserved Histidines of Three Domains
... luciferase molecule (5). The ORF (Figure 1) contains an N-terminal sequence of 111 amino acids, which is homologous to the N-terminal region of the substrate-binding protein, LBP. This is followed by three contiguous domains, designated D1, D2, and D3, which are homologous to one another. At the nuc ...
... luciferase molecule (5). The ORF (Figure 1) contains an N-terminal sequence of 111 amino acids, which is homologous to the N-terminal region of the substrate-binding protein, LBP. This is followed by three contiguous domains, designated D1, D2, and D3, which are homologous to one another. At the nuc ...