Production of recombinant EMA-1 protein and its
... amplified by PCR and subcloned into pET28a expression vector. The full-length sequence was confirmed by DNA sequencing and the ORF encoded a protein of 272 residues of amino acids. Multiple sequence alignment was performed to evaluate the identity of EMA-1 from T. equi (GenBank accession no. DQ25054 ...
... amplified by PCR and subcloned into pET28a expression vector. The full-length sequence was confirmed by DNA sequencing and the ORF encoded a protein of 272 residues of amino acids. Multiple sequence alignment was performed to evaluate the identity of EMA-1 from T. equi (GenBank accession no. DQ25054 ...
Food derived from glufosinate ammonium
... regard to antibiotic resistance genes. Antibiotic resistance genes can be present in some transgenic plants as a result of their use as marker genes to select transformed cells. It is generally accepted that there are no safety concerns with regard to the presence in the food of antibiotic resistanc ...
... regard to antibiotic resistance genes. Antibiotic resistance genes can be present in some transgenic plants as a result of their use as marker genes to select transformed cells. It is generally accepted that there are no safety concerns with regard to the presence in the food of antibiotic resistanc ...
Document
... The following media and protocols have been developed for use with DE3 lysogens such as BL21(DE3) and B834(DE3), which supply T7 RNA polymerase upon induction of the lacUV5 promoter, in combination with pET expression vectors in which expression of the target gene is controlled from the T7lac promot ...
... The following media and protocols have been developed for use with DE3 lysogens such as BL21(DE3) and B834(DE3), which supply T7 RNA polymerase upon induction of the lacUV5 promoter, in combination with pET expression vectors in which expression of the target gene is controlled from the T7lac promot ...
NIHMS88703-supplement-2
... weight instead of adiposity were recorded while mice were on several different diets including a medium high fat diet (44.9% Kcal from fat) and a high sucrose diet (76.5% kcal from carbohydrate). Both male and female Me1 ko mice on a medium high fat diet demonstrated decreased body weight (Figure 1i ...
... weight instead of adiposity were recorded while mice were on several different diets including a medium high fat diet (44.9% Kcal from fat) and a high sucrose diet (76.5% kcal from carbohydrate). Both male and female Me1 ko mice on a medium high fat diet demonstrated decreased body weight (Figure 1i ...
Differential mRNA expression levels and gene sequences of a
... strain relative to that in the susceptible strain. Southern analysis indicated that Pst I or Eco RI restriction sites are different in the two strains. Both a modified gene structure and an increase in expression of carboxylesterase may be responsible for the high level of resistance found in this b ...
... strain relative to that in the susceptible strain. Southern analysis indicated that Pst I or Eco RI restriction sites are different in the two strains. Both a modified gene structure and an increase in expression of carboxylesterase may be responsible for the high level of resistance found in this b ...
Regulation of Elovl and fatty acid metabolism
... (TER) that were found to catalyse the reduction of 3-ketoacyl-CoA and trans-2,3-enoyl-CoA respectively (Moon and Horton, 2003). At that time, Ybr159p and Tsc13p had been identified as analogue reduction enzymes in yeast (Beaudoin et al., 2002; Kohlwein et al., 2001). Recently, Denic and Weissman hav ...
... (TER) that were found to catalyse the reduction of 3-ketoacyl-CoA and trans-2,3-enoyl-CoA respectively (Moon and Horton, 2003). At that time, Ybr159p and Tsc13p had been identified as analogue reduction enzymes in yeast (Beaudoin et al., 2002; Kohlwein et al., 2001). Recently, Denic and Weissman hav ...
Insilico Studies on Taste Receptor Gene (Tas2r38) and Tas2r38
... compounds. In fact, many bitter compounds, including food-borne substances, are toxic, although a relation of bitterness and toxicity has not been established. Bitter perception generally occurs through bitter taste receptors located on the surface of taste cells of the tongue.[1]In vertebrates, bit ...
... compounds. In fact, many bitter compounds, including food-borne substances, are toxic, although a relation of bitterness and toxicity has not been established. Bitter perception generally occurs through bitter taste receptors located on the surface of taste cells of the tongue.[1]In vertebrates, bit ...
... 16. (12 pts) Please do one of the following questions. Choice A: Pretend it’s next Sunday and you just finished the Pittsburgh marathon. As a consequence, your glycogen levels and ATP levels in the liver are quite low. Discuss the process, with the major focus on regulation in your answer, by which ...
Candidate genes and single nucleotide polymorphisms associated
... (Hubbard et al., 2009) were used as reference sequences. The SNP returned by the Maq SNPfilter command were annotated using a collection of command-line scripts (Grant et al., 2011) NGS-SNP (Grant et al., 2011) by assigning a functional class to each SNP (e.g., nonsynonymous) and then providing Ense ...
... (Hubbard et al., 2009) were used as reference sequences. The SNP returned by the Maq SNPfilter command were annotated using a collection of command-line scripts (Grant et al., 2011) NGS-SNP (Grant et al., 2011) by assigning a functional class to each SNP (e.g., nonsynonymous) and then providing Ense ...
Final published version
... in a site-specific and oriented way in the ldhB promoter region results in high expression of the gene, and ultimately, this random event could restore a fully homolactic fermentation profile (20). In addition to the ldhB gene, two other genes, the ldhX and hicD (llmg_0475 in MG1363) genes, encoding ...
... in a site-specific and oriented way in the ldhB promoter region results in high expression of the gene, and ultimately, this random event could restore a fully homolactic fermentation profile (20). In addition to the ldhB gene, two other genes, the ldhX and hicD (llmg_0475 in MG1363) genes, encoding ...
The role of carbohydrate in sperm
... material since a 'constant' supply of recombinant material would be available. However, although biologically-active human recombinant ZP3 produced by Chinese hamster ovary (CHO) cells was first reported in 1993 (Barratt et al., 1993), subsequent purification of biologically active material that pro ...
... material since a 'constant' supply of recombinant material would be available. However, although biologically-active human recombinant ZP3 produced by Chinese hamster ovary (CHO) cells was first reported in 1993 (Barratt et al., 1993), subsequent purification of biologically active material that pro ...
Proteomic profiling of non-obese type 2 diabetic skeletal muscle
... moderate differential expression pattern for 21 proteins, whereby 7 proteins were found to be reduced and 14 proteins to be increased in their abundance in GK tissue. With respect to neuromuscular disorders, the mass spectrometric cataloguing of normal muscle and the profiling of genetic and physiol ...
... moderate differential expression pattern for 21 proteins, whereby 7 proteins were found to be reduced and 14 proteins to be increased in their abundance in GK tissue. With respect to neuromuscular disorders, the mass spectrometric cataloguing of normal muscle and the profiling of genetic and physiol ...
PhoB by Phosphate Stress and Controlled by
... for all manipulations of plasmid and chromosomal DNA. In previous work in which we sequenced the DNA flanking the Tn5B22 insert in RmMSU4, it was determined that the transposon was in the correct orientation for reporting ackA transcription (52). Briefly, total chromosomal DNA was harvested, digeste ...
... for all manipulations of plasmid and chromosomal DNA. In previous work in which we sequenced the DNA flanking the Tn5B22 insert in RmMSU4, it was determined that the transposon was in the correct orientation for reporting ackA transcription (52). Briefly, total chromosomal DNA was harvested, digeste ...
Problems in Protein Biosynthesis - The Journal of General Physiology
... Evidence has been provided that tRNA and ribosomal RNA are synthesized on the DNA template (39-41). The Deciphering of the Code The actual deciphering of the code was based on the following conceptual insights and experimental achievements. For some time ribosomal RNA itself was assumed to be the te ...
... Evidence has been provided that tRNA and ribosomal RNA are synthesized on the DNA template (39-41). The Deciphering of the Code The actual deciphering of the code was based on the following conceptual insights and experimental achievements. For some time ribosomal RNA itself was assumed to be the te ...
Shedding Genomic Ballast: Extensive Parallel Loss of Ancestral
... that are present in certain genomes have been scored as absent. However, there are reasons for believing that, while these factors may have operated in some cases, they are unlikely to be responsible for the overall trends observed. First, the genes included in this analysis were, by definition, cons ...
... that are present in certain genomes have been scored as absent. However, there are reasons for believing that, while these factors may have operated in some cases, they are unlikely to be responsible for the overall trends observed. First, the genes included in this analysis were, by definition, cons ...
Lactic acid bacteria as a cell factory: rerouting of carbon metabolism
... generated by PEP conversion to pyruvate by pyruvate kinase (pyk) (Fig. 2). Recently, the ptsHI operon of L. lactis, encoding the general PTS components HPr and enzyme I, respectively, has been cloned and characterized. HPr plays an important role regulation of sugar utilization, both at the level of ...
... generated by PEP conversion to pyruvate by pyruvate kinase (pyk) (Fig. 2). Recently, the ptsHI operon of L. lactis, encoding the general PTS components HPr and enzyme I, respectively, has been cloned and characterized. HPr plays an important role regulation of sugar utilization, both at the level of ...
Structural and Thermal Stability Characterization of Protein
... to study the % of activity retention after steam sterilization. Substrate O-methoxyphenol and oxidant H2O2 were added to the protein solution. Oxidized product formation was monitored as a function of time in the kinetics mode reading the absorbance at 470n using an HP 8453 diode array spectrophoto ...
... to study the % of activity retention after steam sterilization. Substrate O-methoxyphenol and oxidant H2O2 were added to the protein solution. Oxidized product formation was monitored as a function of time in the kinetics mode reading the absorbance at 470n using an HP 8453 diode array spectrophoto ...
EP 1790660 B1
... [0026] In a fifth approach to heterologous expression, two or more (e.g. 3, 4, 5, 6 or more) proteins of the invention are expressed as a single hybrid protein. It is preferred that no non-Neisserial fusion partner (e.g. GST or poly-His) is used. [0027] This offers two advantages. Firstly, a protein ...
... [0026] In a fifth approach to heterologous expression, two or more (e.g. 3, 4, 5, 6 or more) proteins of the invention are expressed as a single hybrid protein. It is preferred that no non-Neisserial fusion partner (e.g. GST or poly-His) is used. [0027] This offers two advantages. Firstly, a protein ...
PhoR, PhoP and MshC: Three essential proteins of Mycobacterium
... final goal, we desired to express M.tb MshC in E. coli codon-optimized competent cells. This venture has not yet proved successful. Expression of M.tb MshC will allow for crystallization attempts of this protein as well as the determination of kinetic data. ...
... final goal, we desired to express M.tb MshC in E. coli codon-optimized competent cells. This venture has not yet proved successful. Expression of M.tb MshC will allow for crystallization attempts of this protein as well as the determination of kinetic data. ...
Modeling Cell Proliferation Activity of Human Interleukin
... IL-3 Structure, Function, and Experimental Mutagenesis Data • IL-3 promotes the growth of many hematopoietic cell lines • Theoretically, there are 19 × 112 = 2128 possible IL-3 mutants via single residue substitutions at all positions in the structure • Experimental dataset: 630 of these IL-3 mutan ...
... IL-3 Structure, Function, and Experimental Mutagenesis Data • IL-3 promotes the growth of many hematopoietic cell lines • Theoretically, there are 19 × 112 = 2128 possible IL-3 mutants via single residue substitutions at all positions in the structure • Experimental dataset: 630 of these IL-3 mutan ...
Influence of Valine Analogues on Protein Synthesis of Chicken
... The influence of various valine analogues on protein synthesis of chicken embryo myoblasts was examined. Valine and its analogues (D-valine, methylvaline, valinol) were supplemented into Medium 199 containing 20 ng/ml of chicken insulin-like growth factor-I (IGF-I) instead of fetal calf serum (FCS). ...
... The influence of various valine analogues on protein synthesis of chicken embryo myoblasts was examined. Valine and its analogues (D-valine, methylvaline, valinol) were supplemented into Medium 199 containing 20 ng/ml of chicken insulin-like growth factor-I (IGF-I) instead of fetal calf serum (FCS). ...
Experimental Analysis of the Rice Mitochondrial
... The coordination of biochemical processes to perform the functions of mitochondria requires many hundreds of different proteins working together in protein complexes, in two membrane systems, and in several aqueous spaces. The majority of mitochondrial proteins are encoded in the nucleus and transpo ...
... The coordination of biochemical processes to perform the functions of mitochondria requires many hundreds of different proteins working together in protein complexes, in two membrane systems, and in several aqueous spaces. The majority of mitochondrial proteins are encoded in the nucleus and transpo ...
144803525 - BORA
... biosynthesis of catecholamines. The posttranslational modification of its regulatory domain controls the regulation of TH enzyme activity. Different kinases are responsible for the phosphorylation of the enzyme at Thr8, Ser19, Ser31 and Ser40 N terminal residues. It is well established that phosphor ...
... biosynthesis of catecholamines. The posttranslational modification of its regulatory domain controls the regulation of TH enzyme activity. Different kinases are responsible for the phosphorylation of the enzyme at Thr8, Ser19, Ser31 and Ser40 N terminal residues. It is well established that phosphor ...
GENETICS TEST IV - Daytona State College
... BE ABLE TO DEFINE • Structural genes of the lac operon • Genes coding for the primary structure of an enzyme are called structural genes. • The lac operon has three structural genes • lacZ – encodes for -galactosidase, an enzyme that converts the disaccharide lactose to its monosaccharides glucose ...
... BE ABLE TO DEFINE • Structural genes of the lac operon • Genes coding for the primary structure of an enzyme are called structural genes. • The lac operon has three structural genes • lacZ – encodes for -galactosidase, an enzyme that converts the disaccharide lactose to its monosaccharides glucose ...
Synthetic Peptides as Antigens for Antibody Production
... generally be too hydrophobic to consider. The N-terminus of a protein can also prove to be a good candidate sequence, but in our experience is a less reliable choice than the C-terminus and may be modified or truncated. Regions with too high a charge or hydrophilicity are sometimes not as effective ...
... generally be too hydrophobic to consider. The N-terminus of a protein can also prove to be a good candidate sequence, but in our experience is a less reliable choice than the C-terminus and may be modified or truncated. Regions with too high a charge or hydrophilicity are sometimes not as effective ...