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Posttranslational Protein Modiications in Plant
Posttranslational Protein Modiications in Plant

... regulation of plant metabolism by PTMs, in particular for enzymes in primary metabolism (Calvin cycle, glycolysis, and respiration) and the C4 shuttle accommodating photosynthesis in C4 plants (Table III). As will be evident, most of these classic examples involve regulation by (de)phosphorylation, ...
Posttranslational Protein Modiications in Plant
Posttranslational Protein Modiications in Plant

... regulation of plant metabolism by PTMs, in particular for enzymes in primary metabolism (Calvin cycle, glycolysis, and respiration) and the C4 shuttle accommodating photosynthesis in C4 plants (Table III). As will be evident, most of these classic examples involve regulation by (de)phosphorylation, ...
Classification and domain analysis of protein
Classification and domain analysis of protein

... Required for cell-type-specific transcription and signal transduction ...
Origin and evolution of peptide-modifying
Origin and evolution of peptide-modifying

... (HSBAP1; e = 1015) and jumonji-related domain protein 5 (JmjD5; e = 1015) from eukaryotes, and bacterial homologs (e.g. Myxococcus xanthus MXAN_4411; e = 1018). Conversely, profile searches with RPS-BLAST using a position-specific score matrix, which we had previously prepared for the detection of ...
IJEB 48(4) 373-377
IJEB 48(4) 373-377

... revealed the presence of Ala, Asp, Glu, Gly, Ser and Thr. The earlier studies on azo-dye bound peptide revealed that it contained Pro, Leu/Ile, Val and Gly as major components and Glu, Phe, Ser, Ala and Asp as minor components19. Terayama and Takeuchi20 isolated a fraction on hydrolysis of azo-dye b ...
The significance of biochemical and molecular sample integrity in
The significance of biochemical and molecular sample integrity in

... four groups (Table 1). The first group was sacrificed by focused microwave irradiation (Muromachi Kikai, Tokyo, Japan), 1.4 s at 4.5–5 kW (control group). Three groups of animals were sacrificed by cervical dislocation and kept in room temperature (227C) at different time intervals, 1, 3, and 10 min ...
Canine CD34: Cloning of the cDNA and Evaluation
Canine CD34: Cloning of the cDNA and Evaluation

... and D) and Hind111 (C) restriction sites (underlined) were included. Reverse transcription andPCR were performed as previously described.” Samples were electrophoresed through 2% agarose gels and analyzed by ethidium bromide staining andor by probing of Southern blots with caCD34-specific [y-”PIdATP ...
The Transition from Stiff to Compliant Materials in Squid Beaks
The Transition from Stiff to Compliant Materials in Squid Beaks

... 1, B and C). The pigmentation gradient appears to be closely coupled to a distribution of catechols that correspond to proteins containing 3,4dihydroxyphenyl-L-alanine (dopa) (9). Catechol staining (red in Fig. 1D) is evident in the lightly tanned regions and more intensely (though largely obscured ...
MB ChB PHASE I
MB ChB PHASE I

... they cleave adjacent to different amino-acids. Together, they break polypeptides to free amino-acids and short peptides. In addition, a mucosal cell-surface aminopeptidase removes amino-acids one at a time from N termini. ...
Chapter 12
Chapter 12

... • Mutations in the promoter are uninducible and cis-acting. • When mutant and wild-type subunits are present, a single lacI–d mutant subunit can inactivate a ...
Akashi_Gojobori.PNAS02
Akashi_Gojobori.PNAS02

... metabolism. Energy, in the form of high-energy phosphate bonds (‘‘⬃P’’) and reducing power (‘‘H’’), is lost through diversion of intermediates from fueling reactions and further energy is required to convert starting metabolites to amino acids. If a substantial fraction of amino acids are synthesize ...
In vitro gastrointestinal digestion study of a novel bio-tofu
In vitro gastrointestinal digestion study of a novel bio-tofu

... Composite gels containing casein (the main cow milk proteins) and soy proteins are possible to be obtained according to the previous studies (Grygorczyk et al., 2014; Lin, Hill & Corredig, 2012). Formulations containing both soymilk and cow milk proteins provide additional health benefits and also s ...
Ces locus embedded proteins control the non
Ces locus embedded proteins control the non

... enzyme complex Ces that is encoded on a pXO1-like megaplasmid. To decipher the role of the genes adjacent to the structural genes cesA/cesB, coding for the nonribosomal peptide synthetase (NRPS), gene inactivation- and overexpression mutants of the emetic strain F4810/72 were constructed and their i ...
Transcriptomic analysis reveals metabolic switches and
Transcriptomic analysis reveals metabolic switches and

... These environmental switches involve dramatic changes in the physiology of these parasites. In fact, T. cruzi has three main stages during its life cycle: trypomastigotes (infective and non-replicative), amastigotes (replicative and intracellular in the vertebrate host), and epimastigotes (replicati ...
The novel genome organization of the insect picorna
The novel genome organization of the insect picorna

... it was found that two of the capsid proteins were encoded by sequences in ORF-2 (SKPTVQ and VMGEDL). The third Nterminal sequence (ANFQTN) was found to be situated upstream of the first methionine of ORF-2 (see Fig. 1). This sequence is encoded in the same frame ; thus if there were another initiati ...
Biochemical Evidence for the Role of the Waxy Protein fron Pea
Biochemical Evidence for the Role of the Waxy Protein fron Pea

... Dry et al. (1992) compared the amino acid sequence of the mature pea 59-kD protein (named GBSSI in that paper) deduced from the corresponding cDNA sequence with those of the maize Wx and potato Wx proteins and the glycogen synthase of Escherichia coli (see also Preiss, 1991) and could find no obviou ...
Lactococcus lactis as expression host for the biosynthetic
Lactococcus lactis as expression host for the biosynthetic

... replaced by a tyrosine or phenylalanine residue, often without a noticeable effect on protein function. The use of Trp analogues with altered optical properties can further broaden the intrinsic fluorescence approach. A number of Trp analogues like 7AzaTrp (7-azatryptophan), 5-OHTrp (5-hydroxytrypto ...
Amino Acid Starter Kit
Amino Acid Starter Kit

... - Continue to fold you protein making sure that your polar sidechains are also on the outside surface of your protein where they can hydrogen bond with water. - Last, fold your protein so that the two cysteine sidechains are positioned opposite each other on the inside of the protein where they can ...
Global Properties of the Metabolic Map of
Global Properties of the Metabolic Map of

... protein subunits coded by neighboring genes? Interestingly, this seems to be the case for >90% of known heteromeric enzymes (data not shown). For instance, genes for imidazole glycerol phosphate synthase subunits HisH and HisF are located at centisome positions 45.053 and 45.081, respectively. An ex ...
Characterization of the Cobalamin (Vitamin B12) Biosynthetic Genes
Characterization of the Cobalamin (Vitamin B12) Biosynthetic Genes

... encoded in the genomes of Salmonella spp. and other enteric bacteria (23, 93, 98). (ii) Ethanolamine ammonia lyase degrades ethanolamine to acetaldehyde and ammonia. Salmonella spp. can use ethanolamine as a carbon and/or nitrogen source under aerobic conditions when exogenous cobalamin or cobinamid ...


... Choice A: How does the presence of cis double bonds in unsaturated fatty acids affect the phase transition of the membrane? What intermolecular interaction is affected by the presence of these groups in the bilayer? Choice B: Compare and contrast the structure of a membrane protein (e.g. bacteriorho ...
Isolation and Characterization of Rice MADS Box Gene
Isolation and Characterization of Rice MADS Box Gene

... The expression profile of E31864 in Fig. 2 supports its relatedness to floral development in which API is involved. In contrast, E20969 was found in all tissues examined, although the degree of expression was slightly higher in the panicle at meiotic and flowering stages (Fig. 2). E20969 might act m ...
Codon usage in the Mycobacterium tuberculosis corn
Codon usage in the Mycobacterium tuberculosis corn

... although its genomic G C content is rather lower than in these other species (about 65 mol%, compared to about 75 mol YO). Second, M. tuberculosis multiplies almost exclusively in macrophages, with a doubling time of about 24 h : if codon selection were mediated by the need for efficient use of ribo ...
BIOMOLECULES: INTRODUCTION, STRUCTURE AND FUNCTION
BIOMOLECULES: INTRODUCTION, STRUCTURE AND FUNCTION

... The peptide bond is the covalent bond that links amino acids together. It is essentially an amide linkage having certain characteristics that are very important to protein structure. The values of bond lengths and bond angles of various linkages related to a peptide bond are shown in Figure 1. It is ...
Chapter 2
Chapter 2

... imagine an almost limitless variety of peptides. For example, there are 2050 or slightly more than 1.12 ⫻ 1065 possible sequences for polypeptides with just 50 amino acid residues. ...
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Expression vector

An expression vector, otherwise known as an expression construct, is usually a plasmid or virus designed for protein expression in cells. The vector is used to introduce a specific gene into a target cell, and can commandeer the cell's mechanism for protein synthesis to produce the protein encoded by the gene. Expression vectors are the basic tools in biotechnology for the production of proteins.The plasmid is engineered to contain regulatory sequences that act as enhancer and promoter regions and lead to efficient transcription of the gene carried on the expression vector. The goal of a well-designed expression vector is the production of protein, and this may be achieve by the production of significant amount of stable messenger RNA, which can then be translated into protein. The protein may be expressed constitutively, or induced when necessary using an inducer. Escherichia coli is commonly used as the host for protein expression, other cell types however may also be used. An example of the use of expression vector is the production of insulin which is used for medical treatments of diabetes.
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