Functional characterization of LePGT1, a membrane
... of membrane-bound AS-PTs has been reported regardless of origin. In particular, no characterization of plant AS-PTs has been reported. This is mainly because of the difficulty in handling these proteins. Representative of membrane-bound AS-PTs are the PPT [PHB (p-hydroxybenzoic acid) prenyltransfera ...
... of membrane-bound AS-PTs has been reported regardless of origin. In particular, no characterization of plant AS-PTs has been reported. This is mainly because of the difficulty in handling these proteins. Representative of membrane-bound AS-PTs are the PPT [PHB (p-hydroxybenzoic acid) prenyltransfera ...
Gene Section GHRL (ghrelin/obestatin prepropeptide) Atlas of Genetics and Cytogenetics
... decreased gradually along the gastro-intestinal tract and a faint expression was also detected in testis. By Immnunochemistry Ghrelin-immuno reactive neurons were found in the hypothalamic arcuate nucleus. ...
... decreased gradually along the gastro-intestinal tract and a faint expression was also detected in testis. By Immnunochemistry Ghrelin-immuno reactive neurons were found in the hypothalamic arcuate nucleus. ...
Over-expression of UDP-glucose pyrophosphorylase in hybrid
... of UGPase in secondary cell wall biosynthesis in hybrid poplar, and its effects on carbon partitioning. Poplar trees were transformed with UGPase from Acetobacter xylinum under the control of the constitutive (2335S) promoter and were used to evaluate changes in tree growth characteristics, biochemi ...
... of UGPase in secondary cell wall biosynthesis in hybrid poplar, and its effects on carbon partitioning. Poplar trees were transformed with UGPase from Acetobacter xylinum under the control of the constitutive (2335S) promoter and were used to evaluate changes in tree growth characteristics, biochemi ...
Why nature chose phosphate to modify proteins
... larger than that of PO3 4 . In addition, the partial negative charge on the P–O oxygen atoms is approximately 6 per cent greater than for As–O. These chemical differences would alter the structure and properties of macromolecules using arsenate diesters as linkages. For these reasons, a recent repo ...
... larger than that of PO3 4 . In addition, the partial negative charge on the P–O oxygen atoms is approximately 6 per cent greater than for As–O. These chemical differences would alter the structure and properties of macromolecules using arsenate diesters as linkages. For these reasons, a recent repo ...
Isolation and characterization of (S)
... obtained (Kutchan, 1995). Alkaloids are a group of naturally occurring low-molecular weight nitrogenous compounds found in about 20% of plant species. The majority of alkaloids in plants are derived from the amino acids tyrosine, tryptophan and phenylalanine. They are often basic and contain nitroge ...
... obtained (Kutchan, 1995). Alkaloids are a group of naturally occurring low-molecular weight nitrogenous compounds found in about 20% of plant species. The majority of alkaloids in plants are derived from the amino acids tyrosine, tryptophan and phenylalanine. They are often basic and contain nitroge ...
Purified Hexameric Epstein-Barr Virus-Encoded BARF1 Protein for Measuring Anti-BARF1 Antibody
... Cell culture. 293HEK cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM); an EBV-negative NPC-derived cell line, Hone1, was cultured in RPMI medium (Lonza, Breda, Netherlands); and the gastric cancer cell line AGS was cultured in HAM’s nutrient mixture F12 (Sigma-Aldrich, Zwijndrecht, N ...
... Cell culture. 293HEK cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM); an EBV-negative NPC-derived cell line, Hone1, was cultured in RPMI medium (Lonza, Breda, Netherlands); and the gastric cancer cell line AGS was cultured in HAM’s nutrient mixture F12 (Sigma-Aldrich, Zwijndrecht, N ...
Molecular cloning and nucleotide sequence of another variant of the
... H.I.8 is a member of the SLTII family. Since SLTIIva shares with SLTIIv the property of having low cytotoxicity to HeLa cells compared with Vero cells, it is appropriateto consider both toxins as variants of SLTII. SLTIIva M e r s from SLTIIv in that it is more heat-stable. Further, SLTIIv-producing ...
... H.I.8 is a member of the SLTII family. Since SLTIIva shares with SLTIIv the property of having low cytotoxicity to HeLa cells compared with Vero cells, it is appropriateto consider both toxins as variants of SLTII. SLTIIva M e r s from SLTIIv in that it is more heat-stable. Further, SLTIIv-producing ...
Nucleotide Sequence of fruA, the Gene Specifying Enzyme IIfru of
... of these derivatives were separated into membrane and cytoplasmic fractions; these could be tested for the presence of active FPr/Enzyme 111"" (cytoplasmic) and Enzyme IIfru(membrane), by measuring their ability to complement extracts of mutants impaired in specific known components of the fructose- ...
... of these derivatives were separated into membrane and cytoplasmic fractions; these could be tested for the presence of active FPr/Enzyme 111"" (cytoplasmic) and Enzyme IIfru(membrane), by measuring their ability to complement extracts of mutants impaired in specific known components of the fructose- ...
4. Characterisation of novel proteins
... decades. The organism itself has been extensively studied and commercially exploited in forestry, agriculture, including organic food production systems, as well as home gardens. Members of the large family of Bt Cry proteins have been used in the control of mosquitoes that are vectors of human dise ...
... decades. The organism itself has been extensively studied and commercially exploited in forestry, agriculture, including organic food production systems, as well as home gardens. Members of the large family of Bt Cry proteins have been used in the control of mosquitoes that are vectors of human dise ...
Arabidopsis Phosphoglycerate Dehydrogenase1 of
... PGDH Isoenzymes Are Differentially Regulated via Ser Feedback Inhibition To determine substrate affinity and feedback regulation of Arabidopsis PGDH isoenzymes, truncated versions of all three genes lacking putative target peptide sequences were cloned into the pET16b vector (Novagen). The constructs ...
... PGDH Isoenzymes Are Differentially Regulated via Ser Feedback Inhibition To determine substrate affinity and feedback regulation of Arabidopsis PGDH isoenzymes, truncated versions of all three genes lacking putative target peptide sequences were cloned into the pET16b vector (Novagen). The constructs ...
A1060 GM Corn Line 5307 AppR SD1 Safety Assess
... decades. The organism itself has been extensively studied and commercially exploited in forestry, agriculture, including organic food production systems, as well as home gardens. Members of the large family of Bt Cry proteins have been used in the control of mosquitoes that are vectors of human dise ...
... decades. The organism itself has been extensively studied and commercially exploited in forestry, agriculture, including organic food production systems, as well as home gardens. Members of the large family of Bt Cry proteins have been used in the control of mosquitoes that are vectors of human dise ...
A futile metabolic cycle activated in adipocytes by - Zen-Bio
... puzzle. TZDs might have direct effects on muscle and liver; however, the concentration of PPAR-γ is very low in these tissues, particularly skeletal muscle. As a result, much attention has been paid to the effects of TZDs on the adipocyte. Indeed, TZDs regulate the expression of several adipocyte-se ...
... puzzle. TZDs might have direct effects on muscle and liver; however, the concentration of PPAR-γ is very low in these tissues, particularly skeletal muscle. As a result, much attention has been paid to the effects of TZDs on the adipocyte. Indeed, TZDs regulate the expression of several adipocyte-se ...
mTORC1 Activates SREBP-1c and Uncouples Lipogenesis From Gluconeogenesis Please share
... show that SREBP-1c mRNA expression is tightly regulated by mTORC1 under physiological conditions. The identification of mTORC1 as the bifurcation point that separates hepatic lipogenesis from gluconeogenesis represents an important advance in our understanding of the molecular mechanisms linking hepa ...
... show that SREBP-1c mRNA expression is tightly regulated by mTORC1 under physiological conditions. The identification of mTORC1 as the bifurcation point that separates hepatic lipogenesis from gluconeogenesis represents an important advance in our understanding of the molecular mechanisms linking hepa ...
Full Text PDF - Mary Ann Liebert, Inc. publishers
... plasma; and elevated levels of VLCFA are used as a biomarker for the biochemical diagnosis of the disease. Classical inactivation of ABCD1 in the mouse results in late onset neurodegeneration with axonopathy in spinal cord, in the absence of inflammatory demyelination in the brain, resembling the mo ...
... plasma; and elevated levels of VLCFA are used as a biomarker for the biochemical diagnosis of the disease. Classical inactivation of ABCD1 in the mouse results in late onset neurodegeneration with axonopathy in spinal cord, in the absence of inflammatory demyelination in the brain, resembling the mo ...
Journal of Molecular Microbiology and Biotechnology
... 1999], the pps operon was assigned to fengycin biosynthesis, although B. subtilis does not produce this lipopeptide. Interestingly, in the genome of B. subtilis ATCC 6633, the mycosubtilin biosynthesis gene cluster devoted to synthesis of an iturin-like compound, is situated at the same location [Du ...
... 1999], the pps operon was assigned to fengycin biosynthesis, although B. subtilis does not produce this lipopeptide. Interestingly, in the genome of B. subtilis ATCC 6633, the mycosubtilin biosynthesis gene cluster devoted to synthesis of an iturin-like compound, is situated at the same location [Du ...
novel nucleotide carrier proteins of Protochlamydia
... corresponding genes, expressed these heterologously in E. coli and measured nucleotide transport mediated by the recombinant carrier proteins. It has been shown that this heterologous expression system allows to decipher biochemical properties of a wide range of NTT proteins from both, bacteria and ...
... corresponding genes, expressed these heterologously in E. coli and measured nucleotide transport mediated by the recombinant carrier proteins. It has been shown that this heterologous expression system allows to decipher biochemical properties of a wide range of NTT proteins from both, bacteria and ...
Protein Structure - Macmillan Learning
... more compelling. Proteins have wonderfully complex architectures, sculpted over time to perform their tasks to near perfection. The fact that a protein adopts a unique conformation is amazing: despite the astronomical number of ways in which even a small protein could possibly fold, it folds into a ...
... more compelling. Proteins have wonderfully complex architectures, sculpted over time to perform their tasks to near perfection. The fact that a protein adopts a unique conformation is amazing: despite the astronomical number of ways in which even a small protein could possibly fold, it folds into a ...
The Science of Transgenics
... Ex. – modified EPSP synthase gene (encodes a protein that functions even when plant is treated with Roundup) Transgenic – an organism containing a transgene introduced by technological (not breeding) methods ...
... Ex. – modified EPSP synthase gene (encodes a protein that functions even when plant is treated with Roundup) Transgenic – an organism containing a transgene introduced by technological (not breeding) methods ...
NMR IN DRUG DISCOVERY. FROM SCREENING TO STRUCTURE-BASED DESIGN OF
... The last fail-safe mechanism in the mitochondrial pathway are IAP (Inhibitor of Apoptosis Proteins), another important family of anti-apoptotic proteins.[4] Their mission consists in blocking caspase-8 and 9 initiated events; and for this some family members are able to inhibit proteolytic activity ...
... The last fail-safe mechanism in the mitochondrial pathway are IAP (Inhibitor of Apoptosis Proteins), another important family of anti-apoptotic proteins.[4] Their mission consists in blocking caspase-8 and 9 initiated events; and for this some family members are able to inhibit proteolytic activity ...
Sink regulation of photosynthesis
... for the plant as the production of assimilatory power would exceed the rate of its utilization and irradiance, previously optimal for photosynthesis, would become excessive. Evidence suggests that the relative capacities for electron transport and metabolism are evenly matched (Ott et al., 1999). In ...
... for the plant as the production of assimilatory power would exceed the rate of its utilization and irradiance, previously optimal for photosynthesis, would become excessive. Evidence suggests that the relative capacities for electron transport and metabolism are evenly matched (Ott et al., 1999). In ...
Nucleotide Sequence of the Gene Coding for the
... the nitrogenase proteins: 1) Are the amino acid sequences of nitrogenase proteinsconserved to the same extent throughout the polypeptide chains, or is the conservation only limited to specific regions of the proteins? 2) If specific regions are stronglyconserved, are these conservedregionsassociated ...
... the nitrogenase proteins: 1) Are the amino acid sequences of nitrogenase proteinsconserved to the same extent throughout the polypeptide chains, or is the conservation only limited to specific regions of the proteins? 2) If specific regions are stronglyconserved, are these conservedregionsassociated ...
cloning of gs1 gene encodes glutamine synthetase 1 and
... - located isoform (GS1) and plastid – located isoform (GS2). Surveys have shown that GS1 gene was used widely to generate transgenic plants. Effects on the growth characteristics of GS1 transgenic plants have been reported in many researches. In this research, in order to prepare for generating GS1 ...
... - located isoform (GS1) and plastid – located isoform (GS2). Surveys have shown that GS1 gene was used widely to generate transgenic plants. Effects on the growth characteristics of GS1 transgenic plants have been reported in many researches. In this research, in order to prepare for generating GS1 ...
Can correct protein models be identified?
... Atom-13 group, it can be seen that methyl–methyl and methyl–hydrocarbon contacts are slightly correlated with model quality (CC ⳱ 0.2–0.3). This is a fairly low correlation, but compared with the correlations for all other atom– atom contacts, which have an average of 0.05, it is significantly highe ...
... Atom-13 group, it can be seen that methyl–methyl and methyl–hydrocarbon contacts are slightly correlated with model quality (CC ⳱ 0.2–0.3). This is a fairly low correlation, but compared with the correlations for all other atom– atom contacts, which have an average of 0.05, it is significantly highe ...
Comparative proteomic network signatures in seminal plasma of
... Background: Reactive oxygen species (ROS) plays a major role in the pathology of male infertility. It is an independent biomarker of sperm function. Seminal plasma is a natural reservoir of antioxidants responsible for the nourishment, protection, capacitation, and motility of sperm within the fema ...
... Background: Reactive oxygen species (ROS) plays a major role in the pathology of male infertility. It is an independent biomarker of sperm function. Seminal plasma is a natural reservoir of antioxidants responsible for the nourishment, protection, capacitation, and motility of sperm within the fema ...
Purification to homogeneity and partial amino acid sequence of a
... well, with only three out of fifteen amino acids being different (and two of them by only one nucleotide in each codon). INTRODUCTION C^-methylguanine (C^-MeG) is a potent premutagenic lesion that may be formed in DNA following reaction with a methylating carcinogen (2). In animal models, the presen ...
... well, with only three out of fifteen amino acids being different (and two of them by only one nucleotide in each codon). INTRODUCTION C^-methylguanine (C^-MeG) is a potent premutagenic lesion that may be formed in DNA following reaction with a methylating carcinogen (2). In animal models, the presen ...