Improving penicillin biosynthesis in Penicillium chrysogenum
... Genetic engineering of fungal cell factories mainly focuses on manipulating enzymes of the product pathway or primary metabolism. However, despite the use of strong promoters or strains containing the genes of interest in multiple copies, the desired strongly enhanced enzyme levels are often not obt ...
... Genetic engineering of fungal cell factories mainly focuses on manipulating enzymes of the product pathway or primary metabolism. However, despite the use of strong promoters or strains containing the genes of interest in multiple copies, the desired strongly enhanced enzyme levels are often not obt ...
Primary production of protein: I. Comparison of net cellular carbon
... Specifically, there has not yet been a systematic evaluation of conditions required for the precise and accurate quantitation of protein synthesis by marine phytoplankton based on the incorporation of '"C-carbon. Radioisotopic tracers other than "C associated with distinct types of algal cell materi ...
... Specifically, there has not yet been a systematic evaluation of conditions required for the precise and accurate quantitation of protein synthesis by marine phytoplankton based on the incorporation of '"C-carbon. Radioisotopic tracers other than "C associated with distinct types of algal cell materi ...
REGULATION BY EXERCISE OF SKELETAL MUSCLE CONTENT
... of, lactate production by exercising muscle. It was thought that lactate production is mediated by development of muscle hypoxia, and that the improvement in the ability to perform prolonged submaximal exercise in response to training is mediated by increases in v· O2 max and the ability to deliver ...
... of, lactate production by exercising muscle. It was thought that lactate production is mediated by development of muscle hypoxia, and that the improvement in the ability to perform prolonged submaximal exercise in response to training is mediated by increases in v· O2 max and the ability to deliver ...
Figure 7. N-terminus sequence of the predicted
... an helical fold was shown by experimental and computational approaches [39,40]. It should be worth mentioning that these atomic resolution structures has been questioned [41,42] because they are not consistent with previously proposed empirical functional models. However, there are theoretical and e ...
... an helical fold was shown by experimental and computational approaches [39,40]. It should be worth mentioning that these atomic resolution structures has been questioned [41,42] because they are not consistent with previously proposed empirical functional models. However, there are theoretical and e ...
Identification of four small molecular mass proteins in the silk of
... positions of one degenerate forward and two gene-specific reverse primers that were used in cDNA identification by PCR. The doubleunderlined nucleotide in BmSPI 2 was identified as T in one, and as G in the other cDNA clone. The most probable signal peptide cleavage sites (Von Heijne, 1983) are mark ...
... positions of one degenerate forward and two gene-specific reverse primers that were used in cDNA identification by PCR. The doubleunderlined nucleotide in BmSPI 2 was identified as T in one, and as G in the other cDNA clone. The most probable signal peptide cleavage sites (Von Heijne, 1983) are mark ...
A Novel Role for Vitamin K1 in a Tyrosine Phosphorylation
... vitamin K1 concentration in the fetus and newborn is reflected in reduced g-carboxylation of coagulation factors II, VII, IX, and X, Proteins C and S, and bone protein matrix-Gla protein and osteocalcin (7). Although delayed osteocalcin production without rigid skeletal formation may be of benefit t ...
... vitamin K1 concentration in the fetus and newborn is reflected in reduced g-carboxylation of coagulation factors II, VII, IX, and X, Proteins C and S, and bone protein matrix-Gla protein and osteocalcin (7). Although delayed osteocalcin production without rigid skeletal formation may be of benefit t ...
basic laboratory course 3
... chromatography. Proteins are most often separated by column chromatography, in which a mixture of different proteins is passed through a column or tube that contains a matrix material. The separation occurs because proteins interact with various matrices in different ways. In ion-exchange chromatogr ...
... chromatography. Proteins are most often separated by column chromatography, in which a mixture of different proteins is passed through a column or tube that contains a matrix material. The separation occurs because proteins interact with various matrices in different ways. In ion-exchange chromatogr ...
Remodeled Respiration in ndufs4 with Low
... small portion of the Arabidopsis genome is differently expressed in the ndufs4 mutant compared with the wild type (1,479 genes; P # 0.05). We used MapMan (Thimm et al., 2004) with the Ath_AFFY_TAIR7 mapping file to study functional groups of genes (Supplemental Table S2). Processes involving protein ...
... small portion of the Arabidopsis genome is differently expressed in the ndufs4 mutant compared with the wild type (1,479 genes; P # 0.05). We used MapMan (Thimm et al., 2004) with the Ath_AFFY_TAIR7 mapping file to study functional groups of genes (Supplemental Table S2). Processes involving protein ...
... Choice A: Describe how you make this buffer using the fully protonated form of the weak acid. The fraction deprotonated is fA-= R/(1+R), where R=10pH-pKa = 107.0-6.8=1.58, fA-=.603 You would need to add 0.603 equivalents of base, of 0.0603 moles of base. Choice B: The reaction that you are buffering ...
Fluorescence Study of Bovine β-Lactoglobulin
... β-Lactoglobulin contains 2 Trp residues, Trp 19 present in a hydrophobic pocket and Trp 61 present at the surface of the protein near the pocket [1-5]. In order to find out whether both Trp residues contribute to β-lactoglobulin fluorescence or not, time-resolved studies and static quenching were pe ...
... β-Lactoglobulin contains 2 Trp residues, Trp 19 present in a hydrophobic pocket and Trp 61 present at the surface of the protein near the pocket [1-5]. In order to find out whether both Trp residues contribute to β-lactoglobulin fluorescence or not, time-resolved studies and static quenching were pe ...
The Roles of Amino Acids in Milk Yield and Components
... more difficult to predict with accuracy the amino acid (AA) requirements of ruminants for growth and milk production. The current NRC publications for dairy and beef cattle recognise this, and do not explicitly allow for diets to be balanced for essential (EAA) or limiting AA contents, except to rec ...
... more difficult to predict with accuracy the amino acid (AA) requirements of ruminants for growth and milk production. The current NRC publications for dairy and beef cattle recognise this, and do not explicitly allow for diets to be balanced for essential (EAA) or limiting AA contents, except to rec ...
Analyzing Effects of Naturally Occurring Missense Mutations
... the subcellular localization is a mutation that occurs at a signaling region. For example, missense mutations in Otopetrin 1 affects the subcellular location and causes nonsyndromic otoconia agenesis and a subsequent balance defect in mice [102]. Fanconi anemia is a genetic disease associated with th ...
... the subcellular localization is a mutation that occurs at a signaling region. For example, missense mutations in Otopetrin 1 affects the subcellular location and causes nonsyndromic otoconia agenesis and a subsequent balance defect in mice [102]. Fanconi anemia is a genetic disease associated with th ...
Formation of Monoterpenes in Antirrhinum majus
... was evaluated by transferring each open reading frame into the pET-T7 expression system and producing the proteins in Escherichia coli with and without a C-terminal polyhistidine (6xHis) tag extension (see Methods). The proteins thus expressed had no detectable prenyltransferase activity, even after ...
... was evaluated by transferring each open reading frame into the pET-T7 expression system and producing the proteins in Escherichia coli with and without a C-terminal polyhistidine (6xHis) tag extension (see Methods). The proteins thus expressed had no detectable prenyltransferase activity, even after ...
CHAPTER 4 ISOLATION, CHARACTERIZATION AND EXPRESSION OF GA20ox
... LightCycler technique for quantitative reverse transcription (RT)-PCR of the mRNA levels of the gene of interest using SYBR Green (fluorophore that binds double-stranded DNA) to produce fluorescence for detection. Various internal control primers were designed (Table 4.1) based on constitutively ex ...
... LightCycler technique for quantitative reverse transcription (RT)-PCR of the mRNA levels of the gene of interest using SYBR Green (fluorophore that binds double-stranded DNA) to produce fluorescence for detection. Various internal control primers were designed (Table 4.1) based on constitutively ex ...
53 - Lab Times
... vector. In a second step, the open reading frame (ORF) is transferred by homologous recombination from the entry vector to a panel of expression vectors. These are only the very basics of recombinational cloning; there are actually considerable differences between individual systems and kits. ...
... vector. In a second step, the open reading frame (ORF) is transferred by homologous recombination from the entry vector to a panel of expression vectors. These are only the very basics of recombinational cloning; there are actually considerable differences between individual systems and kits. ...
Characterization of Acetyl-CoA Carboxylases in the Basal
... transition, and 300,000 cell equivalents per lane were run for each time point. Two bands are observed transition, and 300,000 cell equivalents per lane were run for each time point. Two bands are observed at 272 kD and 258 kD (Figure 3), which are consistent with, but larger than the estimated siz ...
... transition, and 300,000 cell equivalents per lane were run for each time point. Two bands are observed transition, and 300,000 cell equivalents per lane were run for each time point. Two bands are observed at 272 kD and 258 kD (Figure 3), which are consistent with, but larger than the estimated siz ...
Comparison of the Structure of the Extrinsic 33 kDa Protein from
... structure of the 33 kDa protein may be different, at least in its free form, among different plant species. The cleavage sites of the 33 kDa protein by protease were reported to be different between higher plant and cyanobacterium. The higher plant 33 kDa protein was cleaved at 16Y by chymotrypsin a ...
... structure of the 33 kDa protein may be different, at least in its free form, among different plant species. The cleavage sites of the 33 kDa protein by protease were reported to be different between higher plant and cyanobacterium. The higher plant 33 kDa protein was cleaved at 16Y by chymotrypsin a ...
The potato tuber mitochondrial proteome
... Evaluation of the mitochondria proteome by transient fluorescence assay in tobacco epidermal cell ...
... Evaluation of the mitochondria proteome by transient fluorescence assay in tobacco epidermal cell ...
Principles of transcriptional control in the metabolic
... based on the correlation in their expression profiles. Shown here is the matrix of their pair-wise production (Fig. 3a). Alternatively, isozymes correlations. The cluster of highly correlated genes (orange frame) corresponds to genes that encode the could be dedicated to distinct processes using cen ...
... based on the correlation in their expression profiles. Shown here is the matrix of their pair-wise production (Fig. 3a). Alternatively, isozymes correlations. The cluster of highly correlated genes (orange frame) corresponds to genes that encode the could be dedicated to distinct processes using cen ...
Table S1 Genes with similar expression patterns in Qing2
... Peptidyl-prolyl cis-trans isomerase (cyclophilin) of the endoplasmic reticulum, catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; transcriptionally induced in response to unfolded proteins in the ER ...
... Peptidyl-prolyl cis-trans isomerase (cyclophilin) of the endoplasmic reticulum, catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; transcriptionally induced in response to unfolded proteins in the ER ...
Environmentally Sensitive Fluorescent Sensors Based on Synthetic Peptides Linköping University Post Print
... and -helix (20 peptides, [27]) structures were synthesized. In proteins, the solvent accessible part of these secondary structure elements is often implicated in the recognition of protein partners. Peptides were introduced into separate wells of a microplate, either involving covalent immobilizati ...
... and -helix (20 peptides, [27]) structures were synthesized. In proteins, the solvent accessible part of these secondary structure elements is often implicated in the recognition of protein partners. Peptides were introduced into separate wells of a microplate, either involving covalent immobilizati ...
Biochimica et Biophysica Acta
... Bacteria containing a reporter plasmid and a mutagenized protein expression library were grown on LB agar plates containing ampicillin, chloramphenicol, IPTG, and X-gal. Expression of the repressor plasmid is regulated by an IPTG-dependent promoter, and the amount of IPTG was adjusted to allow maxim ...
... Bacteria containing a reporter plasmid and a mutagenized protein expression library were grown on LB agar plates containing ampicillin, chloramphenicol, IPTG, and X-gal. Expression of the repressor plasmid is regulated by an IPTG-dependent promoter, and the amount of IPTG was adjusted to allow maxim ...
Isolated Spinach Ribulose-1,5-Bisphosphate
... Mol. Biol. 40: 415—439 (1989); and Mullet, “Chloroplast development and gene expression,” Annu. Rev. Plant. Physiol. Plant Mol. Biol. 39: 475—502 (1988). Higher plant Rubisco is a hexadecameric protein composed of eight chloroplast-encoded large subunits (referred to herein as “LS”) and eight nuclea ...
... Mol. Biol. 40: 415—439 (1989); and Mullet, “Chloroplast development and gene expression,” Annu. Rev. Plant. Physiol. Plant Mol. Biol. 39: 475—502 (1988). Higher plant Rubisco is a hexadecameric protein composed of eight chloroplast-encoded large subunits (referred to herein as “LS”) and eight nuclea ...
Types and effects of protein variations. Vihinen
... interactions and other characteristics in several ways. List of the effects would be really long and include e.g. interactions, stability, electrostatic effects, protein packing, local and global structural changes and so on. Many deleterious variants are straightforward to explain, such as large de ...
... interactions and other characteristics in several ways. List of the effects would be really long and include e.g. interactions, stability, electrostatic effects, protein packing, local and global structural changes and so on. Many deleterious variants are straightforward to explain, such as large de ...
Gene Section SPP1 (secreted phosphoprotein 1) Atlas of Genetics and Cytogenetics
... half of the proteins including the RGD motif and the central thrombin cleavage site. ...
... half of the proteins including the RGD motif and the central thrombin cleavage site. ...