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Two Arabidopsis Genes (IPMS1 and IPMS2
Two Arabidopsis Genes (IPMS1 and IPMS2

... Heterologous expression of the Arabidopsis (Arabidopsis thaliana) IPMS1 (At1g18500) and IPMS2 (At1g74040) cDNAs in Escherichia coli yields isopropylmalate synthases (IPMSs; EC 2.3.3.13). These enzymes catalyze the first dedicated step in leucine (Leu) biosynthesis, an aldol-type condensation of acet ...
Role of Dietary Protein in Post-Exercise Muscle Reconditioning
Role of Dietary Protein in Post-Exercise Muscle Reconditioning

... properties, which can be attributed to differences in digestion and absorption kinetics as well as amino acid composition [23, 24]. Whey protein is a soluble protein that is rapidly digested and absorbed following ingestion. In contrast, intact casein tends to clot in the stomach after ingestion, th ...
Full-Text  - Academic Journals
Full-Text - Academic Journals

... countries (Eni et al., 2008), indicating a 50% increase in the number of countries worldwide where CMV infection in yam have been reported. Cucumber mosaic virus is an isometric single-stranded positive-sense tripartite RNA virus belonging to the genus Cucumovirus, family Bromoviridae. It infects ov ...
Translocation Arrest by Reversible Folding of a Precursor Protein
Translocation Arrest by Reversible Folding of a Precursor Protein

... processing peptidase resulting in the formation of an intermediate-sized form. The second cleavage occurs at the outer surface of the inner membrane after retranslocation of the intermediate (Hartl et al., 1987a). In mitochondria ofN. crassa only the first cleavage to the intermediate sized form is ...
Regulation of Galactoside Transport by the PTS
Regulation of Galactoside Transport by the PTS

... Since the glucose effect and diauxic growth were documented in 1940s (Monod, 1942; Gale, 1943), numerous studies have been performed to understand the molecular mechanisms behind those phenomena. It has been demonstrated that uptake of various sugars was inhibited in the presence of glucose in Esche ...
Construction of in vivo infectious clones of PRSV P-YK and W-CI
Construction of in vivo infectious clones of PRSV P-YK and W-CI

... 35SPYK and 35SWCI drived from in vivo infectious clones of p35SPYK and p35SWCI also had the same host range and induced similar symptom to their respective wild type viruses did. Confirmation of the construction of recombinant viruses in infected plants The recombinant viruses P-WP3CP, P-WCICP, P-WN ...
Title Optimization of Amino Acid Parameters for Correspondence of
Title Optimization of Amino Acid Parameters for Correspondence of

... from the Ca coordinatesby picking up C„, successivelygreater than 0.6 for more than ten residues. Then these selected segments shown in Table IV are superposed with. each other, so that all these corresponding segments have the good structure correspondences with r.m.s. deviation, 1.42A, on average. ...
A metaproteomic assessment of winter and summer
A metaproteomic assessment of winter and summer

... A metaproteomic survey of surface coastal waters near Palmer Station on the Antarctic Peninsula, West Antarctica, was performed, revealing marked differences in the functional capacity of summer and winter communities of bacterioplankton. Proteins from Flavobacteria were more abundant in the summer ...
Synthetic human prion protein octapeptide repeat binds to the
Synthetic human prion protein octapeptide repeat binds to the

... disordered N-terminal tail [7]. The helical domain represents a highly ordered compact structure and probably is less susceptible to proteolysis, at least in the beginning of the hydrolytic attack, than the tail. The N-terminal domain of the prion molecule becomes more structured after binding coppe ...
(9E10): sc-40 - Santa Cruz Biotechnology
(9E10): sc-40 - Santa Cruz Biotechnology

... region helix-loop-helix leucine zipper motif (bHLH-Zip) protein, designated Max, specifically associates with c-Myc, N-Myc and L-Myc proteins. The Myc-Max complex binds to DNA in a sequence-specific manner under conditions where neither Max nor Myc exhibits appreciable binding. Max can also form het ...
Protein Structure and Interactions
Protein Structure and Interactions

... Carl C Correll, Biochemistry and Molecular Biology, Rosalind Franklin University of Medicine and Sciences, North Chicago, IL, USA. E-mail: [email protected] Chaperones are generally viewed as proteins that facilitate proper folding of other proteins often by preventing aggregation of folding interm ...
The Mouse Prolactin Gene Family Locus
The Mouse Prolactin Gene Family Locus

... ROLACTIN (PRL) IS a hormone/cytokine responsible for the coordination of a wide range of biological processes in vertebrates. In the mouse, rat, cow, and likely other mammalian species, there are large families of paralogous genes closely related to PRL (1). The proteins encoded by the PRL family ge ...
Novel NCC mutants and functional analysis in a new
Novel NCC mutants and functional analysis in a new

... Gitelman syndrome (GS) is an autosomal recessive disorder characterized by hypokalemic metabolic alkalosis in conjunction with significant hypomagnesemia and hypocalciuria. The GS phenotype is caused by mutations in the solute carrier family 12, member 3 (SLC12A3) gene that encodes the thiazide-sens ...
PDF only - at www.arxiv.org.
PDF only - at www.arxiv.org.

... (Berman, H.M., & Westbrook, J.D. 2004). This, in turn, has created the need for computational methods of structure-based protein function prediction, especially those which can be implemented automatically in high-throughput fashion (Jung, J.W. & Lee, W. 2004; Yakunin, A.F., et al., 2004 ). One of t ...
Scholarly Interest Report
Scholarly Interest Report

... We have taken a molecular approach to investigating these aspects of plant biology. The TCH genes of Arabidopsis are rapidly and strongly upregulated in expression in response to various environmental stimuli, including the seemingly innocuous stimulus of touch. These genes are powerful molecular to ...
Mutations lowering the phosphatase activity of HPr kinase
Mutations lowering the phosphatase activity of HPr kinase

... Experiments aimed at localizing domains containing the Idh kinase or P-Idh phosphatase activity provided no clearcut results, since mutations leading to normal kinase but reduced phosphatase activity affected amino acids in all parts of the protein (Ikeda and La Porte, 1991; Miller et al., 2000). We ...
Role of Dietary Soy Protein in Obesity
Role of Dietary Soy Protein in Obesity

... soybeans varies from 36% to 56% [24-27]. Protein content of soybean from different areas are quantitatively different with those grown in the southern United States having high concentration of crude protein [24]. Differences in crude protein and amino acid composition of soybeans exist both within ...
Evolutionary Relationships and Protein Domain Architecture in an
Evolutionary Relationships and Protein Domain Architecture in an

... al. 1999). We show the presence of a large and diverse family of calpain-like proteins in all three organisms, often organized in syntenic gene clusters. The data provide further evidence for the hypothesis that calpains evolved as a result of modular gene fusion events. The presence of numerous cal ...
Acquisition of 1,000 eubacterial genes physiologically transformed a
Acquisition of 1,000 eubacterial genes physiologically transformed a

... H2 to reduce CO2 to methane (obligate hydrogenotrophic methanogens), thereby generating a chemiosmotic ion gradient for ATP synthesis in their energy metabolism, although some species can generate methane from reduced C1 compounds, or acetate in the case of aceticlastic forms (5–7). Their carbon met ...
Cloning and characterization of an eukaryotic initiation factor
Cloning and characterization of an eukaryotic initiation factor

... phosphorylated at Ser50 (which corresponds to Ser51 in mammalian eIF2a). We found that BeKw can indeed phosphorylate the Ser50 site of eIF-2a subunit (Fig. 4C). In contrast to BeKw, BeKm showed no kinase activity (Fig. 4C) although it was expressed in equivalent amounts in cell lysate (Fig. 4B). The ...
How flexible is α-actinin`s rod domain?
How flexible is α-actinin`s rod domain?

... Abstract: α−actinin, an actin binding protein, plays a Endo, and Ebashi (1967)]. On the other hand, in nonkey role in cell migration, cross-links actin filaments in muscle cells, α-actinin organizes the cortical cytoskelethe Z-disk, and is a major component of contractile mus- ton adjacent to membra ...
Are Hydrophobins and/or Non-Specific Lipid Transfer Proteins
Are Hydrophobins and/or Non-Specific Lipid Transfer Proteins

... However, in brewing technology the formation and stability of foam are important criteria concerning beer quality. Sørensen and co-workers (1993) reported that barley LTP1 is involved in beer foam formation. LTP1 purified from beer created a good beer foam with high potential in a foam assay. In con ...
CMBI
CMBI

... – How do the proteins encoded in genomes interact with each other to produce cells and phenotypes ? – To predict such functional interactions between proteins as there exist e.g. in metabolic pathways, signalling pathways or protein complexes ...
9c63$$mr30 Black separation
9c63$$mr30 Black separation

... Increasing evidence indicates that the oral treponemes play a significant role in the microbial ecology associated with the destructive events of periodontal disease [1 – 5]. Riviere et al. [6, 7] have described pathogen-related oral spirochetes (PROS), some of which are believed to be similar to Tr ...
Comparative genomics of unintrogressed Campylobacter coli clades 2 and 3
Comparative genomics of unintrogressed Campylobacter coli clades 2 and 3

... suggesting they are a potent human pathogen, and may currently be underreported in human infections due to niche separation. Keywords: Campylobacter coli, Comparative genomics, Phylogeny, Gamma glutamyltranspeptidase, Sialyltransferase ...
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Expression vector

An expression vector, otherwise known as an expression construct, is usually a plasmid or virus designed for protein expression in cells. The vector is used to introduce a specific gene into a target cell, and can commandeer the cell's mechanism for protein synthesis to produce the protein encoded by the gene. Expression vectors are the basic tools in biotechnology for the production of proteins.The plasmid is engineered to contain regulatory sequences that act as enhancer and promoter regions and lead to efficient transcription of the gene carried on the expression vector. The goal of a well-designed expression vector is the production of protein, and this may be achieve by the production of significant amount of stable messenger RNA, which can then be translated into protein. The protein may be expressed constitutively, or induced when necessary using an inducer. Escherichia coli is commonly used as the host for protein expression, other cell types however may also be used. An example of the use of expression vector is the production of insulin which is used for medical treatments of diabetes.
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