Production of functional protein hydrolysates from Egyptian
... germinated local Egyptian soybean and lupin flours were hydrolyzed using the enzyme papain. The hydrolysis processes were carried out for 2 h and aliquots were withdrawn at different time intervals. We have analysed the protein hydrolysate after 30 min hydrolysis as an example of a partially hydroly ...
... germinated local Egyptian soybean and lupin flours were hydrolyzed using the enzyme papain. The hydrolysis processes were carried out for 2 h and aliquots were withdrawn at different time intervals. We have analysed the protein hydrolysate after 30 min hydrolysis as an example of a partially hydroly ...
Document
... fications allow to anchor proteins to membranes and to promote protein-protein interactions (i.e. for the Ras superfamily G-proteins). Prenyltranferases are enzymes catalyzing the addition of lipid moieties to proteins and are currently considered as potential targets in cancer therapy. Several cand ...
... fications allow to anchor proteins to membranes and to promote protein-protein interactions (i.e. for the Ras superfamily G-proteins). Prenyltranferases are enzymes catalyzing the addition of lipid moieties to proteins and are currently considered as potential targets in cancer therapy. Several cand ...
Print - Circulation Research
... effects on lysosomal function might be slow to develop, we chose to use an in vitro model which can function stably for many hours to days, i.e., the fetal mouse heart in organ culture.2 A second problem in approaching this question is to choose appropriate agents to interfere selectively with lysos ...
... effects on lysosomal function might be slow to develop, we chose to use an in vitro model which can function stably for many hours to days, i.e., the fetal mouse heart in organ culture.2 A second problem in approaching this question is to choose appropriate agents to interfere selectively with lysos ...
Acyl Carrier Protein (ACP) lmport into Chloroplasts Does not
... smaller form of pre-ACP import is apo-ACP. Two important points emerged from this mutational analysis. First, the larger import product arises from a modification that depends on the presence of serine 38. Second, the phosphopantetheine prosthetic group, which cannot be added to pre-ACPASer, is not ...
... smaller form of pre-ACP import is apo-ACP. Two important points emerged from this mutational analysis. First, the larger import product arises from a modification that depends on the presence of serine 38. Second, the phosphopantetheine prosthetic group, which cannot be added to pre-ACPASer, is not ...
PDF 28 - The Open University
... column, you see that the numbers are in decreasing order, with oxygen being the element that makes up the greatest percentage of the body mass. Including ‘percentage’ in the column heading avoids the need for writing the symbol ‘%’ beside every numerical value. Scanning down that column gives you mo ...
... column, you see that the numbers are in decreasing order, with oxygen being the element that makes up the greatest percentage of the body mass. Including ‘percentage’ in the column heading avoids the need for writing the symbol ‘%’ beside every numerical value. Scanning down that column gives you mo ...
The role of multifunctional M1 metallopeptidases
... † Background Metallopeptidases of the M1 family are found in all phyla (except viruses) and are important in the cell cycle and normal growth and development. M1s often have spatiotemporal expression patterns which allow for strict regulation of activity. Mutations in the genes encoding M1s result i ...
... † Background Metallopeptidases of the M1 family are found in all phyla (except viruses) and are important in the cell cycle and normal growth and development. M1s often have spatiotemporal expression patterns which allow for strict regulation of activity. Mutations in the genes encoding M1s result i ...
Gene Section DNMT1 (DNA (cytosine-5-)-methyltransferase 1)) Atlas of Genetics and Cytogenetics
... suppression of transposable elements in mammalian cells, and chromosomal instability events accompanied by activation of endogenous retroviral elements was also observed in Dnmt1Chip/- mice (Howard et al., ...
... suppression of transposable elements in mammalian cells, and chromosomal instability events accompanied by activation of endogenous retroviral elements was also observed in Dnmt1Chip/- mice (Howard et al., ...
Cell biology of carbohydrate metabolism
... cells; lane 2, extract from 293 cells transfected with HA-tagged Mlx and FLAG-tagged ChREBP; lane 3, same as lane 2 except incubated with antibodies to HA; lane 4, same as lane 2 except incubated with antibodies to FLAG. The arrow indicates the position of the ChREBP·Mlx complex. ...
... cells; lane 2, extract from 293 cells transfected with HA-tagged Mlx and FLAG-tagged ChREBP; lane 3, same as lane 2 except incubated with antibodies to HA; lane 4, same as lane 2 except incubated with antibodies to FLAG. The arrow indicates the position of the ChREBP·Mlx complex. ...
Identification and characterization of the ergochrome gene cluster in
... production was activated in axenic culture so that high concentration of phosphate and low concentration of sucrose induced pigment syntheses. Conclusions: This is the first functional analysis of a secondary metabolite gene cluster in the ergot fungus besides that for the classical ergot alkaloids ...
... production was activated in axenic culture so that high concentration of phosphate and low concentration of sucrose induced pigment syntheses. Conclusions: This is the first functional analysis of a secondary metabolite gene cluster in the ergot fungus besides that for the classical ergot alkaloids ...
Spinalin, a new glycine- and histidine
... sequence of spinalin. There is a single open reading frame of 254 amino acids beginning at nucleotide 126. The start codon ATG is immediately followed by a G residue and preceded at position −3 by an A, which is in agreement with the consensus sequence for eukaryotic translation initiation sites (Ko ...
... sequence of spinalin. There is a single open reading frame of 254 amino acids beginning at nucleotide 126. The start codon ATG is immediately followed by a G residue and preceded at position −3 by an A, which is in agreement with the consensus sequence for eukaryotic translation initiation sites (Ko ...
Red blood cells: proteomics, physiology and metabolism
... further information regarding the proteins from which peptides were derived. e.g. molecular weight (MW) in the case of SDS-PAGE, or location when using membrane specific regimes. For SDS/PAGE fractionation, gels are typically sliced at known MW boundaries prior to in-gel proteolytic digestion and su ...
... further information regarding the proteins from which peptides were derived. e.g. molecular weight (MW) in the case of SDS-PAGE, or location when using membrane specific regimes. For SDS/PAGE fractionation, gels are typically sliced at known MW boundaries prior to in-gel proteolytic digestion and su ...
The proton-linked monocarboxylate transporter (MCT) family
... The derived protein sequences exhibited 25–50 % identity with MCT1 and included one human protein sequence that was more closely related to chicken MCT3 (67 % identity) than MCT1 or MCT2 (43 % and 45 % identity respectively). As none of the many other ESTs found at the time more closely matched chic ...
... The derived protein sequences exhibited 25–50 % identity with MCT1 and included one human protein sequence that was more closely related to chicken MCT3 (67 % identity) than MCT1 or MCT2 (43 % and 45 % identity respectively). As none of the many other ESTs found at the time more closely matched chic ...
Mapping Enzyme Active Sites in Complex Proteomes
... to map the sites of probe labeling on enzymes isolated from whole proteomes. In comparing this approach to gel-based ABPP, several advantages of the former method are apparent. First and foremost, gel-free ABPP consolidates into a single step the identification of both the protein targets of activit ...
... to map the sites of probe labeling on enzymes isolated from whole proteomes. In comparing this approach to gel-based ABPP, several advantages of the former method are apparent. First and foremost, gel-free ABPP consolidates into a single step the identification of both the protein targets of activit ...
Engineering of factors determining alpha-amylase and
... All A-amylases studied have a small residue at this position (Gly, Leu, Ser, Thr or Val), in contrast to CGTases which have a more bulky aromatic residue (Tyr or Phe) at this position, which is highly conserved. Characterization of the F196G mutant CGTase of T. thermosulfurigenes EM1 revealed that, ...
... All A-amylases studied have a small residue at this position (Gly, Leu, Ser, Thr or Val), in contrast to CGTases which have a more bulky aromatic residue (Tyr or Phe) at this position, which is highly conserved. Characterization of the F196G mutant CGTase of T. thermosulfurigenes EM1 revealed that, ...
Theranostics Evolution- and Structure
... is characterised by early onset: it usually appears before 25 years of age and presents as a mild form of hyperglycaemia. In recent years, the number of known GCK mutations has markedly increased. As a result, interpreting which mutations cause a disease or confer susceptibility to a disease and cha ...
... is characterised by early onset: it usually appears before 25 years of age and presents as a mild form of hyperglycaemia. In recent years, the number of known GCK mutations has markedly increased. As a result, interpreting which mutations cause a disease or confer susceptibility to a disease and cha ...
國立清華大學 - 罕見疾病基金會
... genome and incorporated in the peripheral segment of complex I facing the mitochondrial matrix. The NDUFS7 was suppressed in human T-REx-293 cells using the RNA interference (RNAi) technology. The reduction in the NDUFS7 expression caused a slow growth rate in galactose containing medium and increas ...
... genome and incorporated in the peripheral segment of complex I facing the mitochondrial matrix. The NDUFS7 was suppressed in human T-REx-293 cells using the RNA interference (RNAi) technology. The reduction in the NDUFS7 expression caused a slow growth rate in galactose containing medium and increas ...
Heptad repeat sequences are located adjacent to hydrophobic
... paramyxovirus fusion and coronavirus peplomer glycoproteins are reasonably hydrophilic despite the regular pattern of hydrophobic amino acids in positions a and d, consistent with the formation of an extended structure (Cohen & Parry, 1986). The heptad regions of some retroviruses, for example human ...
... paramyxovirus fusion and coronavirus peplomer glycoproteins are reasonably hydrophilic despite the regular pattern of hydrophobic amino acids in positions a and d, consistent with the formation of an extended structure (Cohen & Parry, 1986). The heptad regions of some retroviruses, for example human ...
Dynamics of Protein Tyrosine Nitration and
... 3-Nitrotyrosine formation is associated with cell signaling and disease initiation and progression like neurodegenerative diseases, cardiovascular injury and cancer. It even facilitates in the accumulation of nitrated proteins in vivo [11]. The most common neurodegenerative diseases are Alzheimer's ...
... 3-Nitrotyrosine formation is associated with cell signaling and disease initiation and progression like neurodegenerative diseases, cardiovascular injury and cancer. It even facilitates in the accumulation of nitrated proteins in vivo [11]. The most common neurodegenerative diseases are Alzheimer's ...
Heptad repeat sequences are located adjacent to hydrophobic
... paramyxovirus fusion and coronavirus peplomer glycoproteins are reasonably hydrophilic despite the regular pattern of hydrophobic amino acids in positions a and d, consistent with the formation of an extended structure (Cohen & Parry, 1986). The heptad regions of some retroviruses, for example human ...
... paramyxovirus fusion and coronavirus peplomer glycoproteins are reasonably hydrophilic despite the regular pattern of hydrophobic amino acids in positions a and d, consistent with the formation of an extended structure (Cohen & Parry, 1986). The heptad regions of some retroviruses, for example human ...
Chapter 3 - GEOCITIES.ws
... notes!)_____________________________________________ ___________________________________________________ ___________________________________________________ ___________________________________________________ ___________________________________________________ Organisms ...
... notes!)_____________________________________________ ___________________________________________________ ___________________________________________________ ___________________________________________________ ___________________________________________________ Organisms ...
Multiple linear regression for protein secondary structure prediction
... Prediction of the three-dimensional structure of a protein from its sequence is becoming a pressing problem for many biologists because the discrepancy continues to increase between the number of known protein sequences and the number of experimentally determined structures. The prediction of second ...
... Prediction of the three-dimensional structure of a protein from its sequence is becoming a pressing problem for many biologists because the discrepancy continues to increase between the number of known protein sequences and the number of experimentally determined structures. The prediction of second ...
Characterization of Rice Anthranilate Synthase
... important in the regulation of free Trp concentration, and that mutation of OASA1 to render the encoded protein insensitive to feedback inhibition results in accumulation of Trp at high levels. The OASA1(D323N) transgene may prove useful for the generation of crops with an increased Trp content. ...
... important in the regulation of free Trp concentration, and that mutation of OASA1 to render the encoded protein insensitive to feedback inhibition results in accumulation of Trp at high levels. The OASA1(D323N) transgene may prove useful for the generation of crops with an increased Trp content. ...
Molecular characterization of carnitine palmitoyltransferase 1C Esther Gratacòs i Batlle
... 2.1.6 Solubilization of inclusion bodies ___________________________________ 59 2.2 Yeast ______________________________________________________________ 59 2.2.1 Lithium Acetate mediated yeast transformation _______________________ 60 2.2.2 Protein expression in yeast _______________________________ ...
... 2.1.6 Solubilization of inclusion bodies ___________________________________ 59 2.2 Yeast ______________________________________________________________ 59 2.2.1 Lithium Acetate mediated yeast transformation _______________________ 60 2.2.2 Protein expression in yeast _______________________________ ...
Lecture 2
... adjacent strand through hydrogen bonds. Amino acids in anti-parallel strands on the other hand interact with only one amino acid on an adjacent strand. Protein secondary structures – Turns & Loops: Almost all proteins exhibit a compact, globular structure which is possible only if there are turns or ...
... adjacent strand through hydrogen bonds. Amino acids in anti-parallel strands on the other hand interact with only one amino acid on an adjacent strand. Protein secondary structures – Turns & Loops: Almost all proteins exhibit a compact, globular structure which is possible only if there are turns or ...
Fulltext - Jultika
... University of Oulu, during the years 2001–2008. I would like to express my deepest gratitude to my supervisor Professor Kalervo Hiltunen for his excellent guidance and constant support during this study. He shared with me his hand-on experiences from protein purification from animal tissues to commu ...
... University of Oulu, during the years 2001–2008. I would like to express my deepest gratitude to my supervisor Professor Kalervo Hiltunen for his excellent guidance and constant support during this study. He shared with me his hand-on experiences from protein purification from animal tissues to commu ...