BIOL 1322 General Nutrition
... Understand celiac disease, GERD, IBS, ulcerative colitis, Crohns, peptic ulcer, etc and be able to distinguish between and among Identify where bile is formed, stored and its function Identify where bicarbonate is formed and released and its function Identify the major organs/sphincters/accessory or ...
... Understand celiac disease, GERD, IBS, ulcerative colitis, Crohns, peptic ulcer, etc and be able to distinguish between and among Identify where bile is formed, stored and its function Identify where bicarbonate is formed and released and its function Identify the major organs/sphincters/accessory or ...
Drosophila Forkhead Homologues Are Expressed in
... differentiating N-TeraZ/DI cells. a model system for in vitro human pluripotent development.'* It is also expressed in K562 and Jurkat cells, but it is not expressed in any of the other tissues studied, including total normal human marrow. On the basis of expression, it provides an intriguing connec ...
... differentiating N-TeraZ/DI cells. a model system for in vitro human pluripotent development.'* It is also expressed in K562 and Jurkat cells, but it is not expressed in any of the other tissues studied, including total normal human marrow. On the basis of expression, it provides an intriguing connec ...
Drosophila Forkhead Homologues Are Expressed in
... differentiating N-TeraZ/DI cells. a model system for in vitro human pluripotent development.'* It is also expressed in K562 and Jurkat cells, but it is not expressed in any of the other tissues studied, including total normal human marrow. On the basis of expression, it provides an intriguing connec ...
... differentiating N-TeraZ/DI cells. a model system for in vitro human pluripotent development.'* It is also expressed in K562 and Jurkat cells, but it is not expressed in any of the other tissues studied, including total normal human marrow. On the basis of expression, it provides an intriguing connec ...
Chondroitinase ABC I from Proteus vulgaris: cloning, recombinant
... in 248 µl of 50 mM Tris/HCl and 50 mM sodium acetate, pH 8.0, with 1 mg/ml substrate (0.25 mg/ml for hyaluronan) at 37 ◦C. Product formation was monitored as an increase in absorbance at 232 nm as a function of time in our semi-high-throughput format. Initial rates represent < 10 % substrate turnove ...
... in 248 µl of 50 mM Tris/HCl and 50 mM sodium acetate, pH 8.0, with 1 mg/ml substrate (0.25 mg/ml for hyaluronan) at 37 ◦C. Product formation was monitored as an increase in absorbance at 232 nm as a function of time in our semi-high-throughput format. Initial rates represent < 10 % substrate turnove ...
Sequential Expression of Macromolecule
... Antibiotic production began a t about 20 h, following the rapid decrease in RNA synthesis. Thereafter, antibiotic production was linear for at least 40 h. 'Late' formation of antibiotic synthases The late onset of candicidin synthesis may be due to a delayed formation of candicidin synthases or to a ...
... Antibiotic production began a t about 20 h, following the rapid decrease in RNA synthesis. Thereafter, antibiotic production was linear for at least 40 h. 'Late' formation of antibiotic synthases The late onset of candicidin synthesis may be due to a delayed formation of candicidin synthases or to a ...
A Review of Issues of Dietary Protein Intake in Humans
... diet history, training status, and was undertaken more than 30 y ago. The dangers of excessive protein intake should not be underestimated and have been recognized historically through the excess consumption of lean wild meat by early American explorers leading to a condition referred to as “rabbit ...
... diet history, training status, and was undertaken more than 30 y ago. The dangers of excessive protein intake should not be underestimated and have been recognized historically through the excess consumption of lean wild meat by early American explorers leading to a condition referred to as “rabbit ...
Topology Prediction of Membrane Proteins
... The cell is the essential unit of life and is the fundamental building block in all organisms. Cells are surrounded by membranes, usually a double layer of lipids, which separates them from the outside world. The membrane is a physical barrier that protects the cell from foreign molecules at the sam ...
... The cell is the essential unit of life and is the fundamental building block in all organisms. Cells are surrounded by membranes, usually a double layer of lipids, which separates them from the outside world. The membrane is a physical barrier that protects the cell from foreign molecules at the sam ...
MEDICAL BIOCHEMISTRY Problem Unit One 1999/2000 pH and
... Many molecules become charged (i.e. they become ions) when dissolved in water. Most notable of these are the acids and bases. Positive ions are referred to as cations, and negative ions are anions. The predominant cations in blood plasma is Na+ (making up about 150 meq/L out of a total of 170). The ...
... Many molecules become charged (i.e. they become ions) when dissolved in water. Most notable of these are the acids and bases. Positive ions are referred to as cations, and negative ions are anions. The predominant cations in blood plasma is Na+ (making up about 150 meq/L out of a total of 170). The ...
From Sequence to Structure
... structure (or fold) under physiological conditions. On the other hand, the demands of protein function require that the folded protein should not be too rigid. Presumably because of these constraints, the number of folds adopted by proteins, though large, is limited. Whether the limited number of fo ...
... structure (or fold) under physiological conditions. On the other hand, the demands of protein function require that the folded protein should not be too rigid. Presumably because of these constraints, the number of folds adopted by proteins, though large, is limited. Whether the limited number of fo ...
Passenger Protein Determines Translocation Versus Retention in
... Biolabs). The ligation was then transformed to DH5a competent cells and the plasmid was purified through QIAprep Spin Miniprep kit (Qiagen). The accuracy of the plasmids was checked by dideoxy chain termination method in both the strands from a commercial source (MC Laboratory, San Francisco, CA). C ...
... Biolabs). The ligation was then transformed to DH5a competent cells and the plasmid was purified through QIAprep Spin Miniprep kit (Qiagen). The accuracy of the plasmids was checked by dideoxy chain termination method in both the strands from a commercial source (MC Laboratory, San Francisco, CA). C ...
Print this article
... But still prion diseases continue 1o raise many unanswerable questions to be investigated. One of the unanswerable questions is prion muhiplication. Many prion multiplication models are constantftntlitry is that prion replication requires the interaction of PrPt-PrP". The studies of ch more attentio ...
... But still prion diseases continue 1o raise many unanswerable questions to be investigated. One of the unanswerable questions is prion muhiplication. Many prion multiplication models are constantftntlitry is that prion replication requires the interaction of PrPt-PrP". The studies of ch more attentio ...
Structure and biosynthesis of the signal
... Two oligonucleotides were synthesized, the sequences of which were based on those of two of the tryptic peptides (see Materials and Methods). The probes were end-labelled and used to screen a MDCK-cell cDNA library cloned into the vector pTEX [I41 (Herz et al., unpublished results). MDCK cells have ...
... Two oligonucleotides were synthesized, the sequences of which were based on those of two of the tryptic peptides (see Materials and Methods). The probes were end-labelled and used to screen a MDCK-cell cDNA library cloned into the vector pTEX [I41 (Herz et al., unpublished results). MDCK cells have ...
Short-Term Overexpression of a Constitutively Active Form of AMP
... have led to the identification of AMPK as a major pharmacological target for the treatment of metabolic disorders. The antidiabetic agents metformin and thiazolidinediones (5,6) activate AMPK, providing support for the targeting of AMPK in drug development. Studies in animal models of type 2 diabete ...
... have led to the identification of AMPK as a major pharmacological target for the treatment of metabolic disorders. The antidiabetic agents metformin and thiazolidinediones (5,6) activate AMPK, providing support for the targeting of AMPK in drug development. Studies in animal models of type 2 diabete ...
paper describing HSSP
... Link between structure and sequence databases. Fortunately, many proteins in the database of known sequences are similar in sequence to a protein of known structure and this fact can be exploited to close the gap in the size of the two databases. For example, there are currently more than 100 known ...
... Link between structure and sequence databases. Fortunately, many proteins in the database of known sequences are similar in sequence to a protein of known structure and this fact can be exploited to close the gap in the size of the two databases. For example, there are currently more than 100 known ...
Protein structural class prediction using predicted secondary
... The object of these research is to develop methods which can predict the structural class of a protein accurately. Generally if a method follows the SCOP classification scheme [3], then it classifies a protein into one of the following main four structural classes: all-α, all-β, α+β, and α/β. If a m ...
... The object of these research is to develop methods which can predict the structural class of a protein accurately. Generally if a method follows the SCOP classification scheme [3], then it classifies a protein into one of the following main four structural classes: all-α, all-β, α+β, and α/β. If a m ...
Trans-10, cis-12 conjugated linoleic acid reduces neutral lipid
... embryos (n = 15 each groups) free of cumulus cells were fixed in a 500 μL of 2% glutaraldehyde and 2% formaldehyde solution at 4°C for at least 24 h. They were transferred to individual Eppendorf tubes (1 embryo/tube) containing 30 μL of a solution with 10 μg/mL Nile Red (Molecular Probes, Inc., Eug ...
... embryos (n = 15 each groups) free of cumulus cells were fixed in a 500 μL of 2% glutaraldehyde and 2% formaldehyde solution at 4°C for at least 24 h. They were transferred to individual Eppendorf tubes (1 embryo/tube) containing 30 μL of a solution with 10 μg/mL Nile Red (Molecular Probes, Inc., Eug ...
Processing of the Presequence of the Schizosaccharomyces pombe
... processing by MPP was slowed down considerably by converting Arg-21 to Gly (21) was imported into S. pombe mitochondria. The altered precursor protein is processed very slowly to intermediate, therefore allowing precursor to accumulate inside the mitochondria. If the gel is then exposed for longer p ...
... processing by MPP was slowed down considerably by converting Arg-21 to Gly (21) was imported into S. pombe mitochondria. The altered precursor protein is processed very slowly to intermediate, therefore allowing precursor to accumulate inside the mitochondria. If the gel is then exposed for longer p ...
Metal ion reconstitution studies of yeast copper
... Yeast apo CuZnSOD has no tryptophan residues, one tyrosine residue, and one disulfide bond per subunit, resulting in a calculated ε280 of 3230 M –1 cm –1/dimer. Bovine apo CuZnSOD has identical numbers of tryptophan, tyrosine, and cystine residues [7], and therefore it is expected to have the same e ...
... Yeast apo CuZnSOD has no tryptophan residues, one tyrosine residue, and one disulfide bond per subunit, resulting in a calculated ε280 of 3230 M –1 cm –1/dimer. Bovine apo CuZnSOD has identical numbers of tryptophan, tyrosine, and cystine residues [7], and therefore it is expected to have the same e ...
Molecular Characterization of a Hamster Oviduct
... of the mature form of the HOGP region. The amino acid sequence of HOGP appeared to have eight potential N-glycosylation sites. Northern blot analysis revealed that a single message of approximately 2.5 kb was present inoviductal RNA but not inthe RNA of several other hamster tissues. The HOGP showed ...
... of the mature form of the HOGP region. The amino acid sequence of HOGP appeared to have eight potential N-glycosylation sites. Northern blot analysis revealed that a single message of approximately 2.5 kb was present inoviductal RNA but not inthe RNA of several other hamster tissues. The HOGP showed ...
The sequence of human serum albumin cDNA and its expression in
... corresponding to amino acid codons no. 175-179). Although not employed in the construction of the mature HSA expression plasmid, this cDNA clone (P-14) allowed determination of the DNA sequence of the "prepro" peptide coding and 5' non-coding regions of HSA mRNA. The mature HSA mRNA sequence was joi ...
... corresponding to amino acid codons no. 175-179). Although not employed in the construction of the mature HSA expression plasmid, this cDNA clone (P-14) allowed determination of the DNA sequence of the "prepro" peptide coding and 5' non-coding regions of HSA mRNA. The mature HSA mRNA sequence was joi ...
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... protein (FLAP). LTA4 is converted to LTB4, by LTA4-‐hydrolase (LTA4H) or to LTC4 by LTC4-‐synthase (LTC4S). LTs act on cells through plasma membrane bound G-‐protein coupled receptors found on leukocytes, ...
... protein (FLAP). LTA4 is converted to LTB4, by LTA4-‐hydrolase (LTA4H) or to LTC4 by LTC4-‐synthase (LTC4S). LTs act on cells through plasma membrane bound G-‐protein coupled receptors found on leukocytes, ...
the Gene Ontology
... are over-represented or under-represented. Grouping gene products by biological function. Relating a protein’s location to its function. Focusing on particular biological pathways and functions (hypothesis-driven data interrogation). ...
... are over-represented or under-represented. Grouping gene products by biological function. Relating a protein’s location to its function. Focusing on particular biological pathways and functions (hypothesis-driven data interrogation). ...
Selection at the Wobble Position of Codons Read by the Same tRNA
... trinsically biased by the choice of particular codons within cognate pairs and the evaluation of translational accuracy requirements through the comparison of orthologous genes from wholly sequenced genomes. This allowed us to distinguish between efficiency (i.e., the optimization of the speed and e ...
... trinsically biased by the choice of particular codons within cognate pairs and the evaluation of translational accuracy requirements through the comparison of orthologous genes from wholly sequenced genomes. This allowed us to distinguish between efficiency (i.e., the optimization of the speed and e ...