Lactobacilli carry cryptic genes encoding peptidase
... analysis. With reverse transcription-PCR, however, the presence of monocistronic orfZ transcripts was established. The orfZ gene could also be overexpressed in E. coli using the vector pKK223-3. The size of t h e protein synthesized, 41 kDa, confirmed the molecular mass of OrfZ calculated according ...
... analysis. With reverse transcription-PCR, however, the presence of monocistronic orfZ transcripts was established. The orfZ gene could also be overexpressed in E. coli using the vector pKK223-3. The size of t h e protein synthesized, 41 kDa, confirmed the molecular mass of OrfZ calculated according ...
Regulators of Ubiquitin Dependent Protein Degradation in the
... that all subunits of the complex are crucial for the deneddylase function. (ii) The putative mammalian Den1 homolog of the fungus (DenA) was proven to exhibit deneddylase activity in vivo. For the first time, this deneddylase was successfully deleted in an organism. Loss of denA in A. nidulans leads ...
... that all subunits of the complex are crucial for the deneddylase function. (ii) The putative mammalian Den1 homolog of the fungus (DenA) was proven to exhibit deneddylase activity in vivo. For the first time, this deneddylase was successfully deleted in an organism. Loss of denA in A. nidulans leads ...
PpASCL, a moss ortholog of antherspecific chalcone synthaselike
... biosynthesis pathway (Tang et al., 2009; Grienenberger et al., 2010). Expression of these genes is tightly regulated spatially and temporally so that their expression is initiated at the tetrad stage and restricted to the tapetal cells and microspores at the time of exine deposition. In agreement wi ...
... biosynthesis pathway (Tang et al., 2009; Grienenberger et al., 2010). Expression of these genes is tightly regulated spatially and temporally so that their expression is initiated at the tetrad stage and restricted to the tapetal cells and microspores at the time of exine deposition. In agreement wi ...
Lactobacillus plantarum - UEF Electronic Publications
... Lactic acid bacteria (LAB) are a heterogeneous group of organisms found in a wide range of environmental niches due to their large adaptation capacity. They are responsible for the fermentation of many fermented food and feed products because they can transform sugars into organic acids and decrease ...
... Lactic acid bacteria (LAB) are a heterogeneous group of organisms found in a wide range of environmental niches due to their large adaptation capacity. They are responsible for the fermentation of many fermented food and feed products because they can transform sugars into organic acids and decrease ...
Proteolytic processing in the secretory pathway of Aspergillus niger
... proteases. A brief explanation of these four groups is given here; a more elaborate description is given by van den Hombergh (1996). Each group has a different proteolytic mechanism and therefore different enzymatic characteristics and inhibitors. The cysteine proteases are a rare group of proteases ...
... proteases. A brief explanation of these four groups is given here; a more elaborate description is given by van den Hombergh (1996). Each group has a different proteolytic mechanism and therefore different enzymatic characteristics and inhibitors. The cysteine proteases are a rare group of proteases ...
Adjeitey_Cyril _Nii-Klu_2013_ thesis
... contrast, glucose uptake into skeletal muscle, food intake and energy expenditure was increased with the expression of UCP1. Finally, proton leaks through UCP1 were determined to be increased in isolated mitochondria from transgenic versus wild-type mice. Taken together these results indicate a comp ...
... contrast, glucose uptake into skeletal muscle, food intake and energy expenditure was increased with the expression of UCP1. Finally, proton leaks through UCP1 were determined to be increased in isolated mitochondria from transgenic versus wild-type mice. Taken together these results indicate a comp ...
Cardiac troponin I gene knockout - University of Wisconsin–Madison
... tissue that survives and attempts to maintain organ function.7–9 Interpretation of the effects of TnI loss in animal models of cardiac disease is often complicated by other cellular changes that occur in parallel.5– 8 On the other hand, selective removal of TnI has been reported,11,12 but this appro ...
... tissue that survives and attempts to maintain organ function.7–9 Interpretation of the effects of TnI loss in animal models of cardiac disease is often complicated by other cellular changes that occur in parallel.5– 8 On the other hand, selective removal of TnI has been reported,11,12 but this appro ...
Engineering primary metabolic pathways of industrial
... genes that confer a prominent phenotype when the responsible mutation(s) are anywhere on the genome, but not at the place of the coding gene itself. For example, over-expression of certain sigma factors enhanced protein production in stationary phase (Weikert et al., 2000). Screening of libraries to ...
... genes that confer a prominent phenotype when the responsible mutation(s) are anywhere on the genome, but not at the place of the coding gene itself. For example, over-expression of certain sigma factors enhanced protein production in stationary phase (Weikert et al., 2000). Screening of libraries to ...
NOVEL SPERM-SPECIFIC GLYCOLYTIC ISOZYMES GENERATED
... spermatogenesis indicates that glycolysis is essential for sperm motility and male fertility. This pathway is compartmentalized in the principal piece of the sperm flagellum, where several spermatogenic cell-specific isozymes are bound to a cytoskeletal structure known as the fibrous sheath. Fructos ...
... spermatogenesis indicates that glycolysis is essential for sperm motility and male fertility. This pathway is compartmentalized in the principal piece of the sperm flagellum, where several spermatogenic cell-specific isozymes are bound to a cytoskeletal structure known as the fibrous sheath. Fructos ...
Isotope-labeled Peptides
... In techniques such as ICAT (Isotope Coded Affinity Tags) and iTRAQ (isobaric Tags for Relative and Absolute Quantification), peptides that are generated by proteolytic digests of cellular lysates are covalently bound to isotopically labeled tags that have different masses. In ICAT an isotopically co ...
... In techniques such as ICAT (Isotope Coded Affinity Tags) and iTRAQ (isobaric Tags for Relative and Absolute Quantification), peptides that are generated by proteolytic digests of cellular lysates are covalently bound to isotopically labeled tags that have different masses. In ICAT an isotopically co ...
Proteins - The Open University
... full text before using any of the content. We believe the primary barrier to accessing high-quality educational experiences is cost, which is why we aim to publish as much free content as possible under an open licence. If it proves difficult to release content under our preferred Creative Commons l ...
... full text before using any of the content. We believe the primary barrier to accessing high-quality educational experiences is cost, which is why we aim to publish as much free content as possible under an open licence. If it proves difficult to release content under our preferred Creative Commons l ...
Proteolytic Degradation of Hemoglobin in the Intestine of the Human
... specific primers were designed for 5' and 3' rapid amplification of cDNA ends (RACE) using adult N. americanus RNA (GeneRacer; Invitrogen), as described elsewhere [19]. The full-length cDNA sequence of Na-mep-1 was obtained by creating a consensus consisting of the 5' and 3' RACE products and the BG ...
... specific primers were designed for 5' and 3' rapid amplification of cDNA ends (RACE) using adult N. americanus RNA (GeneRacer; Invitrogen), as described elsewhere [19]. The full-length cDNA sequence of Na-mep-1 was obtained by creating a consensus consisting of the 5' and 3' RACE products and the BG ...
Metabolomic and flux‐balance analysis of age‐related decline of
... sources of carbohydrate fuel in flight muscles in many Diptera, such as bees and blowflies (Childress et al, 1970; Suarez et al, 2005), and are most likely to be so in D. melanogaster as well. The large deposits of glycogen in flight muscle of flies, the depletion of these reserves after prolonged flight ...
... sources of carbohydrate fuel in flight muscles in many Diptera, such as bees and blowflies (Childress et al, 1970; Suarez et al, 2005), and are most likely to be so in D. melanogaster as well. The large deposits of glycogen in flight muscle of flies, the depletion of these reserves after prolonged flight ...
Yeast lipid metabolism at a glance
... During the last decades, lipids have gained much attention due to their involvement in health and disease. Lipids are required for the formation of membranes and contribute to many different processes such as cell signaling, energy supply, and cell death. Various organelles such as the endoplasmic r ...
... During the last decades, lipids have gained much attention due to their involvement in health and disease. Lipids are required for the formation of membranes and contribute to many different processes such as cell signaling, energy supply, and cell death. Various organelles such as the endoplasmic r ...
Control of Hepatic Gluconeogenesis During the Transition Period
... determine the relative flux of carbon to gluconeogenesis or glycolysis respectively. After subsequent isomerization of fructose-6-phosphate to glucose-6-phosphate the relative activities of glucokinase and glucose-6-phosphatase (G6Pase) determine the availability of free glucose for release from li ...
... determine the relative flux of carbon to gluconeogenesis or glycolysis respectively. After subsequent isomerization of fructose-6-phosphate to glucose-6-phosphate the relative activities of glucokinase and glucose-6-phosphatase (G6Pase) determine the availability of free glucose for release from li ...
Functional Plant Biology
... of A. glutinosa using microarray-based gene expression data (Tables 3, 4; based on Alloisio et al. 2010). While caution must be exercised in interpreting metadata derived from two different experiments (Tables 2–4), it can be seen that for Frankia in symbiosis with A. glutinosa, glnA and glnII expre ...
... of A. glutinosa using microarray-based gene expression data (Tables 3, 4; based on Alloisio et al. 2010). While caution must be exercised in interpreting metadata derived from two different experiments (Tables 2–4), it can be seen that for Frankia in symbiosis with A. glutinosa, glnA and glnII expre ...
Transcriptional Regulation of Metabolism
... biochemists. They first achieved the identification of enzymatic steps, their functional characterization, and the discovery of regulatory loops with which they are associated. For decades, allosteric controls linked to substrate availability constituted the best of our knowledge of metabolic contro ...
... biochemists. They first achieved the identification of enzymatic steps, their functional characterization, and the discovery of regulatory loops with which they are associated. For decades, allosteric controls linked to substrate availability constituted the best of our knowledge of metabolic contro ...
calculating the structure-based phylogenetic relationship
... City for funding my tuition, offering services and programs that improve my professional development, and for offering superior classroom instruction. Furthermore, I would like to extend a special acknowledgement to Karen Bame, Ph.D. for her immeasurable assistance. ...
... City for funding my tuition, offering services and programs that improve my professional development, and for offering superior classroom instruction. Furthermore, I would like to extend a special acknowledgement to Karen Bame, Ph.D. for her immeasurable assistance. ...
Isolation of Proteins which Interact with Phospholipase A2 (PLA
... The aim of the work was to isolate proteins which interact with phospholipase A2 (PLA2) from human serum after myocardial infarction. During this study, the effect of flavonoid inhibitors from the extract of Bidens Tripartita was examined. First, the paper describes phytochemical characterisation of ...
... The aim of the work was to isolate proteins which interact with phospholipase A2 (PLA2) from human serum after myocardial infarction. During this study, the effect of flavonoid inhibitors from the extract of Bidens Tripartita was examined. First, the paper describes phytochemical characterisation of ...
Molecular cloning of a cDNA encoding a novel Ca2+
... [2,13]. Furthermore, CAN has signi¢cant homology to these nucleases. We thus consider it unlikely that the detected activity resulted from the endogenous E. coli nuclease. It is most likely that CAN is an intracellular nuclease in Arabidopsis. Intracellular nucleases play critical roles in diverse c ...
... [2,13]. Furthermore, CAN has signi¢cant homology to these nucleases. We thus consider it unlikely that the detected activity resulted from the endogenous E. coli nuclease. It is most likely that CAN is an intracellular nuclease in Arabidopsis. Intracellular nucleases play critical roles in diverse c ...
Focus Issue on Plastid Biology Update Novel
... 2. The structure of the redox regulatory dithiol-disulfide network Four main routes of redox regulation exist in chloroplasts, namely via (1) Fd directly, (2) NADPH, (3) thioredoxin (Trx) system and (4) glutathione/glutaredoxin (Fig. 2) (Dietz, 2008). Thioredoxins are reduced by a specific enzyme, t ...
... 2. The structure of the redox regulatory dithiol-disulfide network Four main routes of redox regulation exist in chloroplasts, namely via (1) Fd directly, (2) NADPH, (3) thioredoxin (Trx) system and (4) glutathione/glutaredoxin (Fig. 2) (Dietz, 2008). Thioredoxins are reduced by a specific enzyme, t ...
The Interaction between Dietary Protein and Bone Health
... for a higher protein diet for bone health [21]. Wengreen et al. recruited subjects aged 50-89 with previous hip fractures and matched them with controls of the same age and sex in a case-control study. The 2501 subjects’ diets were assessed using a picturesort food frequency questionnaire. Protein i ...
... for a higher protein diet for bone health [21]. Wengreen et al. recruited subjects aged 50-89 with previous hip fractures and matched them with controls of the same age and sex in a case-control study. The 2501 subjects’ diets were assessed using a picturesort food frequency questionnaire. Protein i ...
Journal of Agricultural and Food Chemistry
... Gorinstein et al., 1996). There also are investigations showing comparisons among soybean, oats, and amaranth in relation to nutritional, structural, and functional properties (Gorinstein, 1993; Marcone and Yada, 1992; Segura-Nieto et al., 1994). To date, there is no information on the relative stab ...
... Gorinstein et al., 1996). There also are investigations showing comparisons among soybean, oats, and amaranth in relation to nutritional, structural, and functional properties (Gorinstein, 1993; Marcone and Yada, 1992; Segura-Nieto et al., 1994). To date, there is no information on the relative stab ...
The Importance of Cardiolipin Synthase for Mitochondrial
... their association with CL in the plant kingdom is unclear. Here, we characterize three mutants disrupted in the Arabidopsis CLS single-copy gene, named cls mutants. In vitro, germinating seedlings of cls mutants are able to develop under low-illumination conditions only, and all three have a slow-gr ...
... their association with CL in the plant kingdom is unclear. Here, we characterize three mutants disrupted in the Arabidopsis CLS single-copy gene, named cls mutants. In vitro, germinating seedlings of cls mutants are able to develop under low-illumination conditions only, and all three have a slow-gr ...
... The hydrophobic effect is less important for DNA since the bases are polar and largely solvent exposed. (1 1/2 pts) Electrostatic interactions, which have little effect on protein stability, have a large effect on the stability of dsDNA due to charge repulsion between the phosphate groups on each st ...