1 Chapter 1 Introduction 1.1 – Introduction Proteins are
... proteins and peptides that contribute to their structure, stability and activity have been elucidated through an enormous body of experimental work that includes spectroscopic and kinetic measurements in response to changes in temperature, pH, and composition of solvents. Central to this endeavor ha ...
... proteins and peptides that contribute to their structure, stability and activity have been elucidated through an enormous body of experimental work that includes spectroscopic and kinetic measurements in response to changes in temperature, pH, and composition of solvents. Central to this endeavor ha ...
Annotation guidelines - Systems Biology and Bioinformatics
... (i) Two polytopic membrane proteins, NarK and NarU, are assumed to transport nitrite out of the Escherichia coli cytoplasm. Examples of (h) and (i) contain words that can potentially be GP, vitamin B6-enzyme in (h) and Two polytopic membrane proteins in (i). In this particular case, both are not ann ...
... (i) Two polytopic membrane proteins, NarK and NarU, are assumed to transport nitrite out of the Escherichia coli cytoplasm. Examples of (h) and (i) contain words that can potentially be GP, vitamin B6-enzyme in (h) and Two polytopic membrane proteins in (i). In this particular case, both are not ann ...
STRUCTURAL INSIGHTS INTO NOVEL MICROBIAL METALLOENZYMES
... and catalytic potential of a metal ion cofactor, as well as the conservation of 3-dimensional fold and structural features in proteins with similar functions. The structural and biochemical characterization of three novel microbial metalloenzymes are presented; two Escherichia coli hypothetical prot ...
... and catalytic potential of a metal ion cofactor, as well as the conservation of 3-dimensional fold and structural features in proteins with similar functions. The structural and biochemical characterization of three novel microbial metalloenzymes are presented; two Escherichia coli hypothetical prot ...
ribosomal defects in a mutant deficient in the yajl homolog of the
... acyl-CoA dehydrogenase FadE, and proline oxidase PutA, all of which transfer electrons from substrates to quinones without using NAD/NADH (Figure 2). The expression levels of mRNAs coding for respiratory dehydrogenases were determined by DNA array experiments (Figure 3A). Mutant/parental strain gene ...
... acyl-CoA dehydrogenase FadE, and proline oxidase PutA, all of which transfer electrons from substrates to quinones without using NAD/NADH (Figure 2). The expression levels of mRNAs coding for respiratory dehydrogenases were determined by DNA array experiments (Figure 3A). Mutant/parental strain gene ...
The Amino Acid Sequence Contains Leucine-Rich
... GPV from 10l2platelets. This high yield also seems to have an advantage over the method reported by Berndt and Phillips or Zafar and Walz (100 pg from 10l2 platelets), although the yield seems to be dependent on freshness of platelet samples (data not shown) as reported by Berndt and Phillips.I4 The ...
... GPV from 10l2platelets. This high yield also seems to have an advantage over the method reported by Berndt and Phillips or Zafar and Walz (100 pg from 10l2 platelets), although the yield seems to be dependent on freshness of platelet samples (data not shown) as reported by Berndt and Phillips.I4 The ...
Abiotic stress in plants: Late Embryogenesis Abundant proteins Imen Amara
... transduction pathways biochemical modulation and proteomic machinery which lead to the survival or death of the affected plants (Ahuja I 2010; Xiong et al. 2002; Yamaguchi-Shinozaki and Shinozaki 2006). In Arabidopsis gene networks of stress-inducible genes have been identified and have been classif ...
... transduction pathways biochemical modulation and proteomic machinery which lead to the survival or death of the affected plants (Ahuja I 2010; Xiong et al. 2002; Yamaguchi-Shinozaki and Shinozaki 2006). In Arabidopsis gene networks of stress-inducible genes have been identified and have been classif ...
Journal of Bacteriology
... generated by gene splicing using the SOE method (18). This method assists with avoiding possible polar effects caused by interrupted reading frames. SOE PCR fusion products were generated using the primers listed in Table S1 in the supplemental material, the spectinomycin resistance gene of pIC333, ...
... generated by gene splicing using the SOE method (18). This method assists with avoiding possible polar effects caused by interrupted reading frames. SOE PCR fusion products were generated using the primers listed in Table S1 in the supplemental material, the spectinomycin resistance gene of pIC333, ...
Jasmonate Biosynthesis, Perception and Function in Plant
... 2008; Yan et al., 2012). Jasmonates are formed from the LOX-catalyzed peroxidation of trienoic fatty acids at carbon atom 13 to form 13-hydroperoxide, which is modified to an allene oxide fatty acid and subsequently cyclized to the compound 12-oxo-phytodienoic acid (OPDA). Jasmonic acid (JA) is synt ...
... 2008; Yan et al., 2012). Jasmonates are formed from the LOX-catalyzed peroxidation of trienoic fatty acids at carbon atom 13 to form 13-hydroperoxide, which is modified to an allene oxide fatty acid and subsequently cyclized to the compound 12-oxo-phytodienoic acid (OPDA). Jasmonic acid (JA) is synt ...
- BioMedSearch
... turn, is the starting material for the production of a wide range of products including Tamiflu, an orally effective anti-influenza agent (see [16]). E. coli, overexpressing PEPS, are widely used in the biosynthesis of shikimic acid as is over-expression of 3-deoxy-D-arabino-heptulosonic acid 7-phos ...
... turn, is the starting material for the production of a wide range of products including Tamiflu, an orally effective anti-influenza agent (see [16]). E. coli, overexpressing PEPS, are widely used in the biosynthesis of shikimic acid as is over-expression of 3-deoxy-D-arabino-heptulosonic acid 7-phos ...
RuBisCO in Non-Photosynthetic Alga Euglena longa: Divergent
... two different subunits. The only known exceptions were documented in dinoflagellates and chromerid algae, where RuBisCO comprises just one nuclear genome-encoded subunit (form II RuBisCO). In these lineages, the Rbc gene was acquired from a bacterium by horizontal gene transfer [3]. In all other cas ...
... two different subunits. The only known exceptions were documented in dinoflagellates and chromerid algae, where RuBisCO comprises just one nuclear genome-encoded subunit (form II RuBisCO). In these lineages, the Rbc gene was acquired from a bacterium by horizontal gene transfer [3]. In all other cas ...
Transcriptome analysis of the two unrelated fungal
... As mentioned above, fungi have likely obtained βlactam biosynthesis genes through horizontal gene transfer from bacteria. This process has further consequences for regulating expression of these bacterial genes in a eukaryotic host. Prokaryotic genes must be adapted to the eukaryotic host system and ...
... As mentioned above, fungi have likely obtained βlactam biosynthesis genes through horizontal gene transfer from bacteria. This process has further consequences for regulating expression of these bacterial genes in a eukaryotic host. Prokaryotic genes must be adapted to the eukaryotic host system and ...
Translation of Cyclin mRNA Is Necessary for Extracts of Activated
... study cell-free extracts that require protein synthesis to enter mitosis and permit more than one round of DNA replication (Lohka and Masui, 1983, 1984; Hutchison et al., 1987; Blow and Laskey, 1988). If it could be shown that cyclin synthesis is necessary and sufficient to promote mitosis in vitro, ...
... study cell-free extracts that require protein synthesis to enter mitosis and permit more than one round of DNA replication (Lohka and Masui, 1983, 1984; Hutchison et al., 1987; Blow and Laskey, 1988). If it could be shown that cyclin synthesis is necessary and sufficient to promote mitosis in vitro, ...
Nucleus-Encoded Genes for Plastid
... cDNA sequences produced from an ongoing Helicosporidium sp. (strain AT2000, originally isolated from the black fly, Simulium jonesii) EST project. Genes were identified for further characterization based on the following three criteria: (i) their association with pathways known to occur in the plast ...
... cDNA sequences produced from an ongoing Helicosporidium sp. (strain AT2000, originally isolated from the black fly, Simulium jonesii) EST project. Genes were identified for further characterization based on the following three criteria: (i) their association with pathways known to occur in the plast ...
Nature inspired platforms for production of acetyl
... infrastructures3. Advanced biofuels are produced from nonfood cellulosic biomass, including but not limited to wheat straw, forest waste, and energy crops such as switchgrass. These feedstocks are either low cost agricultural byproducts or fast-growing, easily cultivated crops that provide more abun ...
... infrastructures3. Advanced biofuels are produced from nonfood cellulosic biomass, including but not limited to wheat straw, forest waste, and energy crops such as switchgrass. These feedstocks are either low cost agricultural byproducts or fast-growing, easily cultivated crops that provide more abun ...
ProteoChem`s Current Catalogue.
... Protein crosslinking reagents possess reactive moieties specific to various functional groups (sulfhydryls, amines, carbohydrates, etc.) on proteins, peptides, or other molecular complexes. The atoms separating a crosslinker’s reactive groups, and eventually the conjugated proteins, form the “spacer ...
... Protein crosslinking reagents possess reactive moieties specific to various functional groups (sulfhydryls, amines, carbohydrates, etc.) on proteins, peptides, or other molecular complexes. The atoms separating a crosslinker’s reactive groups, and eventually the conjugated proteins, form the “spacer ...
Engineering Cytosolic Acetyl-CoA Metabolism in Saccharomyces
... lack of cytosolic acetyl-CoA for lipid biosynthesis. Its growth inability on glucose could be restored through directed evolution, which was explained by an in-frame internal deletion in MTH1 (MTH1-∆T). The MTH1-∆T allele resulted in reduced glucose uptake, which may attenuate the repression of resp ...
... lack of cytosolic acetyl-CoA for lipid biosynthesis. Its growth inability on glucose could be restored through directed evolution, which was explained by an in-frame internal deletion in MTH1 (MTH1-∆T). The MTH1-∆T allele resulted in reduced glucose uptake, which may attenuate the repression of resp ...
Lecture 3 Isoelectric Focusing
... It will become -ve (pH>pI and migrate to the anode (+electrode attracts -ion)) As it passes through the gel, eg when it reaches pH 9 region, it becomes less -vely charged (pH not so much >pI) (+electrode does not attract protein so much – protein slows down) When reaches the pH 8 region, protein cha ...
... It will become -ve (pH>pI and migrate to the anode (+electrode attracts -ion)) As it passes through the gel, eg when it reaches pH 9 region, it becomes less -vely charged (pH not so much >pI) (+electrode does not attract protein so much – protein slows down) When reaches the pH 8 region, protein cha ...
Hydrolysis of a Series of Synthetic Peptide Substrates by the Human
... picornaviruses, including human rhinoviruses. However there have been relatively few comprehensive studies on the rhinovirus 3C protein (Libby et al., 1988). To ascertain not only whether this protein expressed similar proteolytic activity but also whether it is a suitable target for antiviral chemo ...
... picornaviruses, including human rhinoviruses. However there have been relatively few comprehensive studies on the rhinovirus 3C protein (Libby et al., 1988). To ascertain not only whether this protein expressed similar proteolytic activity but also whether it is a suitable target for antiviral chemo ...
Sequence - BIOTEC - Biotechnology Center TU Dresden
... n Archival databanks of biological information n DNA and protein sequences including annotations n Nucleic acid and protein structures including annotations n Protein expression patterns ...
... n Archival databanks of biological information n DNA and protein sequences including annotations n Nucleic acid and protein structures including annotations n Protein expression patterns ...
Statistical potential-based amino acid similarity
... these two approaches are not necessarily mutually exclusive, and they are often combined as terms in the scoring function of “ab initio” or “de novo” protein structure prediction algorithms.9 –12 Although both approaches have been making steady progress in the past years, the latter approach is more ...
... these two approaches are not necessarily mutually exclusive, and they are often combined as terms in the scoring function of “ab initio” or “de novo” protein structure prediction algorithms.9 –12 Although both approaches have been making steady progress in the past years, the latter approach is more ...
PROTEIN SUBCELLULAR LOCALIZATION
... The selected features are shown with their weights. Features with 0 weight for all datasets were omitted. “12 ” and “22 ” indicate the quadratic term rather was selected with a weight of 1 or 2 respectively. The amino acid content features (by one letter code) selected for the three datasets were “A ...
... The selected features are shown with their weights. Features with 0 weight for all datasets were omitted. “12 ” and “22 ” indicate the quadratic term rather was selected with a weight of 1 or 2 respectively. The amino acid content features (by one letter code) selected for the three datasets were “A ...
New lysosomal acid lipase gene mutants explain the phenotype of
... tion at the 21 position of exon 8 donor site (the 59 splice site of the corresponding intron) that results in exon skipping and the subsequent loss of 24 amino acids from the protein is the most frequent defect observed in patients with CESD (4, 7–9). Skipping of the same exon has also been observed ...
... tion at the 21 position of exon 8 donor site (the 59 splice site of the corresponding intron) that results in exon skipping and the subsequent loss of 24 amino acids from the protein is the most frequent defect observed in patients with CESD (4, 7–9). Skipping of the same exon has also been observed ...
Novel regulatory roles of omega-3 fatty acids in metabolic pathways
... Mice fed a high n-3 PUFA diet showed a lower expression of regucalcin compared to mice fed a low n-3 PUFA diet (Table 1). Regucalcin is mainly expressed in rodent liver, and higher expression of regucalcin is linked to metabolic disorders including diabetes and lipid metabolism [38,39]. Regucalcin h ...
... Mice fed a high n-3 PUFA diet showed a lower expression of regucalcin compared to mice fed a low n-3 PUFA diet (Table 1). Regucalcin is mainly expressed in rodent liver, and higher expression of regucalcin is linked to metabolic disorders including diabetes and lipid metabolism [38,39]. Regucalcin h ...
Solution Blowing of Soy Protein Fibers
... (0.008 – 0.013 wt%) and Mg (0.025 – 0.10 wt%). EDS spectra from SEM image collection was employed to detect the unique atomic markers from soy protein isolate to differentiate from nylon-6 in a monolithic blend of nanofibers such as shown in Figure 1c. Figure 2a shows the EDS spectra for a formic ac ...
... (0.008 – 0.013 wt%) and Mg (0.025 – 0.10 wt%). EDS spectra from SEM image collection was employed to detect the unique atomic markers from soy protein isolate to differentiate from nylon-6 in a monolithic blend of nanofibers such as shown in Figure 1c. Figure 2a shows the EDS spectra for a formic ac ...
A family of diatom-like silicon transporters in the siliceous loricate
... two pairs of GXQ-containing motifs have been noted previously, the first in TMDs 2 and 3, the second located in TMDs 7 and 8, but symmetrically orientated with respect to the membrane polarity [13]. In our alignment, the C-terminal motifs of the pair contained GRQ, apart from one CRQ motif (Shionodi ...
... two pairs of GXQ-containing motifs have been noted previously, the first in TMDs 2 and 3, the second located in TMDs 7 and 8, but symmetrically orientated with respect to the membrane polarity [13]. In our alignment, the C-terminal motifs of the pair contained GRQ, apart from one CRQ motif (Shionodi ...