for growth. fermentation end products and genes required growth of
... and acetoin are not major products of E. coli fermentation and no formate peak of around 8 ppm was detected for B. subtilis (data not shown). The possibility that formate is converted quickly to CO2 and H2 is unlikely since no gas production was observed. These results indicate that B. subtilis perf ...
... and acetoin are not major products of E. coli fermentation and no formate peak of around 8 ppm was detected for B. subtilis (data not shown). The possibility that formate is converted quickly to CO2 and H2 is unlikely since no gas production was observed. These results indicate that B. subtilis perf ...
E-mail: - HAL
... Many research groups have designed fragment libraries or structural alphabets to try to describe the local structural features of known protein structures more accurately.17-29 These libraries or alphabets correspond to finite sets of protein structural fragments. They can be differentiated accordin ...
... Many research groups have designed fragment libraries or structural alphabets to try to describe the local structural features of known protein structures more accurately.17-29 These libraries or alphabets correspond to finite sets of protein structural fragments. They can be differentiated accordin ...
Marinus Pilon, Ph - Colorado State University
... Gillece, P, Pilon M and Römisch, K (2000) The protein translocation channel mediates glycopeptide export across the endoplasmic reticulum membrane. Proc. Natl. Acad. Sci. USA 97, 4609-4614 Pilon M, and Schekman R (1999). Protein Translocation: How Hsp70 pulls it off. Cell 97, 679-682 Pilon M, Römisc ...
... Gillece, P, Pilon M and Römisch, K (2000) The protein translocation channel mediates glycopeptide export across the endoplasmic reticulum membrane. Proc. Natl. Acad. Sci. USA 97, 4609-4614 Pilon M, and Schekman R (1999). Protein Translocation: How Hsp70 pulls it off. Cell 97, 679-682 Pilon M, Römisc ...
a-Aminoadipate aminotransferase from an extremely
... et al., 1999); this has already been shown in part by Irvin & Bhattacharjee (1998). In lower eukaryotes, the enzyme a-aminoadipate aminotransferase (AAAAT; EC 2.6.1.39), which catalyses the transamination reaction using 2-oxoadipate (2-OA) as an amino acceptor to yield AAA, is suggested to be involv ...
... et al., 1999); this has already been shown in part by Irvin & Bhattacharjee (1998). In lower eukaryotes, the enzyme a-aminoadipate aminotransferase (AAAAT; EC 2.6.1.39), which catalyses the transamination reaction using 2-oxoadipate (2-OA) as an amino acceptor to yield AAA, is suggested to be involv ...
Get PDF - Wiley Online Library
... amino acids. In fact, many metabolic processes required for the conversion of higher carbon sources are downregulated and the bacteroids depend on the supply of dicarboxylic acids by the plant to obtain the energy required for nitrogen fixation (Lodwig et al., 2003). Biological nitrogen fixation is ...
... amino acids. In fact, many metabolic processes required for the conversion of higher carbon sources are downregulated and the bacteroids depend on the supply of dicarboxylic acids by the plant to obtain the energy required for nitrogen fixation (Lodwig et al., 2003). Biological nitrogen fixation is ...
as a PDF
... control gene expression. While regulation of transcription initiation is a common regulatory strategy, it is now apparent that this is only the starting point. Bacteria have developed several sophisticated regulatory mechanisms that allow the organism to modulate gene expression after transcription ...
... control gene expression. While regulation of transcription initiation is a common regulatory strategy, it is now apparent that this is only the starting point. Bacteria have developed several sophisticated regulatory mechanisms that allow the organism to modulate gene expression after transcription ...
Cis-trans isomerization of omega dihedrals in Proteins
... constraints in term of sequence and local conformation. Finally we made an overview of the numerous molecular dynamics studies and prediction methodologies already developed to take into account this structural feature in the research area of protein modeling. Many cis peptide bonds have not been re ...
... constraints in term of sequence and local conformation. Finally we made an overview of the numerous molecular dynamics studies and prediction methodologies already developed to take into account this structural feature in the research area of protein modeling. Many cis peptide bonds have not been re ...
KNOX1 genes regulate lignin deposition and composition
... Rodriguez et al., 2010; Spinelli et al., 2011) and monocot (Palatnik et al., 2007; Mateos et al., 2010) model systems, respectively. Gibberellin is a potent target for plant breeding and crop improvement since some agronomic traits such as fiber quality (Larkan et al., 2013), tree height, and growth ...
... Rodriguez et al., 2010; Spinelli et al., 2011) and monocot (Palatnik et al., 2007; Mateos et al., 2010) model systems, respectively. Gibberellin is a potent target for plant breeding and crop improvement since some agronomic traits such as fiber quality (Larkan et al., 2013), tree height, and growth ...
Selected Reaction Monitoring (SRM) to determine protein
... mixture and estimate its abundance by quantitation of peptides derived from its enzymatic digestion. In this approach a triple quadropole (Q or q) mass spectrometer (QqQ) transmits a peptide precursor ion in Q1 that is then fragmented in q2 and a single peptide fragment ion is selected in Q3 for qu ...
... mixture and estimate its abundance by quantitation of peptides derived from its enzymatic digestion. In this approach a triple quadropole (Q or q) mass spectrometer (QqQ) transmits a peptide precursor ion in Q1 that is then fragmented in q2 and a single peptide fragment ion is selected in Q3 for qu ...
Attachment 2 - Food Standards Australia New Zealand
... beets are processed into white sugar, pulp and molasses. Each of these fractions has multiple uses for food, feed or industrial applications, but the principal food products derived from sugar beet are sugar and (to a much lesser degree) molasses. DESCRIPTION OF THE GENETIC MODIFICATION Method used ...
... beets are processed into white sugar, pulp and molasses. Each of these fractions has multiple uses for food, feed or industrial applications, but the principal food products derived from sugar beet are sugar and (to a much lesser degree) molasses. DESCRIPTION OF THE GENETIC MODIFICATION Method used ...
A fluorophore ligase for site-specific protein labeling inside living cells
... Third, we characterized the sequence specificity of both coumarin ligases. It was unclear if remodeling of the lipoic acid binding pocket would affect LplA’s recognition of peptide and protein substrates. In addition, mammalian cells express protein substrates for their own mitochondrial lipoic acid ...
... Third, we characterized the sequence specificity of both coumarin ligases. It was unclear if remodeling of the lipoic acid binding pocket would affect LplA’s recognition of peptide and protein substrates. In addition, mammalian cells express protein substrates for their own mitochondrial lipoic acid ...
Sequence-based predictions of membrane-protein topology, homology and insertion
... annotation of new DNA and protein sequences are often based on the assumption that only the sequence is known, and try to predict various features such as DNA exons and introns, protein domains or subcellular location using only sequence as input. Thus, there is a certain rationale for developing pr ...
... annotation of new DNA and protein sequences are often based on the assumption that only the sequence is known, and try to predict various features such as DNA exons and introns, protein domains or subcellular location using only sequence as input. Thus, there is a certain rationale for developing pr ...
Biomarker discovery for psychiatric disorders
... Although over the last century tremendous progress has been made in the therapy and mortality decrease of devastating conditions such as cancer and cardiopathies, no decrease has been observed in mortality rates or overall prevalence of psychiatric disorders (Kessler et al., 2005). It is therefore n ...
... Although over the last century tremendous progress has been made in the therapy and mortality decrease of devastating conditions such as cancer and cardiopathies, no decrease has been observed in mortality rates or overall prevalence of psychiatric disorders (Kessler et al., 2005). It is therefore n ...
Altered pathogenicity, immunity, tissue tropism and 3` genome
... infectious bronchitis virus (IBV) belongs to the group 3 coronaviruses. It primarily causes respiratory disease in domestic fowl, although it also replicates on epithelial surfaces of the alimentary tract, oviduct, and kidney, and is one of the most economically important pathogens in the poultry in ...
... infectious bronchitis virus (IBV) belongs to the group 3 coronaviruses. It primarily causes respiratory disease in domestic fowl, although it also replicates on epithelial surfaces of the alimentary tract, oviduct, and kidney, and is one of the most economically important pathogens in the poultry in ...
A E M , Feb. 2004, p. 999–1007
... incorporated into the polymer. Short-chain-length (SCL) PHAs consist of monomer units of C3 to C5, mediumchain-length (MCL) PHAs consist of monomer units of C6 to C14, and SCL-MCL PHAs consist of monomers ranging in size from C4 to C14. Although previous studies using recombinant Escherichia coli ha ...
... incorporated into the polymer. Short-chain-length (SCL) PHAs consist of monomer units of C3 to C5, mediumchain-length (MCL) PHAs consist of monomer units of C6 to C14, and SCL-MCL PHAs consist of monomers ranging in size from C4 to C14. Although previous studies using recombinant Escherichia coli ha ...
Development and application of a positive
... which hampers easy selection of such mutants (6). Therefore, relatively simple procedures that are routinely used in other organisms to analyse gene function by targeted gene disruption, such as complementation of function through restoration of the disrupted gene or by the introduction of transgene ...
... which hampers easy selection of such mutants (6). Therefore, relatively simple procedures that are routinely used in other organisms to analyse gene function by targeted gene disruption, such as complementation of function through restoration of the disrupted gene or by the introduction of transgene ...
A mRNA localized to the vegetal cortex of Xenopus
... Pasceri, 1989; Yuge et al., 1990). Embryological experiments have also mapped the specification of endoderm (Wylie et al., 1987), the germ cell lineage (Whitington and Dixon, 1975) and mesoderm (Nieuwkoop, 1973) to the vegetal hemisphere. Of all these processes, only mesoderm induction has been char ...
... Pasceri, 1989; Yuge et al., 1990). Embryological experiments have also mapped the specification of endoderm (Wylie et al., 1987), the germ cell lineage (Whitington and Dixon, 1975) and mesoderm (Nieuwkoop, 1973) to the vegetal hemisphere. Of all these processes, only mesoderm induction has been char ...
Directed mutagenesis of the Trypanosoma cruzi trans
... The sequences of several genes encoding T. cruzi TS and TS-like proteins have been reported (Pereira et al., 1991; Pollevick et al., 1991; Uemura et al., 1992), and the predicted amino acid sequences of the subclass of enzymatically active gene products indicate that the parasite proteins have struc ...
... The sequences of several genes encoding T. cruzi TS and TS-like proteins have been reported (Pereira et al., 1991; Pollevick et al., 1991; Uemura et al., 1992), and the predicted amino acid sequences of the subclass of enzymatically active gene products indicate that the parasite proteins have struc ...
Chapter 18
... Necrotic core results from apoptosis and necrosis, increased proteolytic activity and lipid accumulation. ...
... Necrotic core results from apoptosis and necrosis, increased proteolytic activity and lipid accumulation. ...
The Effects of Whey Protein and Leucine Supplementation
... 7). Furthermore, the magnitude of the increases in strength and hypertrophy are related to the volume of the resistance training program (8,9). For example, recent studies have shown that an increase in the volume of resistance training from one set to two or more sets results in the recruitment of ...
... 7). Furthermore, the magnitude of the increases in strength and hypertrophy are related to the volume of the resistance training program (8,9). For example, recent studies have shown that an increase in the volume of resistance training from one set to two or more sets results in the recruitment of ...
Conservation of the metabolomic response to starvation across two divergent microbes.
... consisting of nitrogen decreases. Elemental analysis of nitrogenstarved yeast revealed this to be the case (Fig. 2C). None of the other starvation conditions resulted in substantial changes in cellular elemental composition [see supporting information (SI) Table 1]. Thus, a major metabolic differenc ...
... consisting of nitrogen decreases. Elemental analysis of nitrogenstarved yeast revealed this to be the case (Fig. 2C). None of the other starvation conditions resulted in substantial changes in cellular elemental composition [see supporting information (SI) Table 1]. Thus, a major metabolic differenc ...
Conservation of the metabolomic response to starvation across two divergent microbes.
... consisting of nitrogen decreases. Elemental analysis of nitrogenstarved yeast revealed this to be the case (Fig. 2C). None of the other starvation conditions resulted in substantial changes in cellular elemental composition [see supporting information (SI) Table 1]. Thus, a major metabolic differenc ...
... consisting of nitrogen decreases. Elemental analysis of nitrogenstarved yeast revealed this to be the case (Fig. 2C). None of the other starvation conditions resulted in substantial changes in cellular elemental composition [see supporting information (SI) Table 1]. Thus, a major metabolic differenc ...
Isolation and Characterization of Two Polypeptides
... Proteins that form the tonofilaments in cutaneous epithelia have been isolated from calf snout (8, 12, 16, 17), hoof epidermis (18), human epidermis (21), and rat epidermis (2, 23) . A large fraction of the extractable protein in such epithelia (as much as 60% in stratum corneum of epidermis) consis ...
... Proteins that form the tonofilaments in cutaneous epithelia have been isolated from calf snout (8, 12, 16, 17), hoof epidermis (18), human epidermis (21), and rat epidermis (2, 23) . A large fraction of the extractable protein in such epithelia (as much as 60% in stratum corneum of epidermis) consis ...
Inhibition of Wound-Induced Accumulation of
... overnight for 14 h at 280°C. The signals on the x-ray films were quantified densitometrically with a densitometer (model GS-670, Bio-Rad), and the AOS transcript level was plotted as a percentage of maximum transcript level. The northern blots and JA quantitations shown in the various figures are re ...
... overnight for 14 h at 280°C. The signals on the x-ray films were quantified densitometrically with a densitometer (model GS-670, Bio-Rad), and the AOS transcript level was plotted as a percentage of maximum transcript level. The northern blots and JA quantitations shown in the various figures are re ...
Current understanding of fatty acid biosynthesis and the acyl carrier
... both a dehydration as well as an isomerization reaction. The isomerase function is exclusively performed on C10 substrates. FabA converts β-hydroxydecanoyl-ACP into trans-2-decenoylACP and subsequently isomerizes this fatty acyl intermediate to cis-3-decenoyl-ACP (Figure 4) [36,37]. Subsequently, th ...
... both a dehydration as well as an isomerization reaction. The isomerase function is exclusively performed on C10 substrates. FabA converts β-hydroxydecanoyl-ACP into trans-2-decenoylACP and subsequently isomerizes this fatty acyl intermediate to cis-3-decenoyl-ACP (Figure 4) [36,37]. Subsequently, th ...