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Terpene Biosynthesis
Terpene Biosynthesis

... deacylation of HMG-CoA to mevalonate (MVA) 3 via mevaldate 2 and employs two equivalents of NADPH as reductant Scheme 1. This enzyme activity provides an important control mechanism for the flow of metabolites into mevalonate and, especially, into steroid biosynthesis and its study continues to stimu ...
Life Inside a Microtubule
Life Inside a Microtubule

... stimuli. Unfortunately, many of the studies are correlational and do not report a direct relationship between a given kinase and actin phosphorylation1. For example, Ser and Tyr residues on actin are phosphorylated in response to insulin via unknown kinases, leading to reduced DNAse I binding1 (Fig. ...
PDF
PDF

... 973,749 bp (termed FERRO_contig000001), 962,266 bp (FERRO_contig000002) and 59,722 bp (FERRO_contig000001). The gaps between these contigs are thought to contain ribosomal RNA gene clusters since both ends of FERRO_contig000001 and one end of each of the other two contigs contain ribosomal RNA genes ...
Pollen Exine Pattern Formation is Dependent on Three Major
Pollen Exine Pattern Formation is Dependent on Three Major

... simple inner nexine layer and the outer sexine portion, which determines the sculptured exine structure. Biochemical studies have shown that a constituent of the exine precursor, sporopollenin, potentially contains polymers of fatty acid, phenylpropanoids and phenolics derivatives. On the other hand ...
Soybean Meal – An Exceptional Protein Source
Soybean Meal – An Exceptional Protein Source

... Soybean meal has long been considered an outstanding source of supplemental protein in diets for livestock and poultry. In fact, soybean meal is sometimes referred to as the "gold standard" because other protein sources are often compared to it. Soybean meal is rich in highly digestible protein, and ...
IDENTIFICATION AND CHARACTERIZATION OF SYNAPTONEMAL
IDENTIFICATION AND CHARACTERIZATION OF SYNAPTONEMAL

... without heterologous synapsis or synaptic adjustment, to form a complete set of entirely synapsed bivalents, have a better chance of producing viable meiotic products than cells which do not (e.g. De Boer and De Jong, 1989). According to this hypothesis mutation of genes encoding structural componen ...
Structural and Functional Studies on Proteinaceous Metallocarboxypeptidase Inhibitors  Joan López Arolas
Structural and Functional Studies on Proteinaceous Metallocarboxypeptidase Inhibitors Joan López Arolas

... as well as its mechanism of inhibition toward metallocarboxypeptidases. Applications of the information derived from this study are discussed. This work was done in Munich between the Department of Clinical Biochemistry and the Department of Structure Research, Max-Planck Institute for Biochemistry, ...
A motif and amino acid bias bioinformatics
A motif and amino acid bias bioinformatics

... Downloaded from on June 17, 2017 - Published by www.plantphysiol.org Copyright © 2017 American Society of Plant Biologists. All rights reserved. ...
Metabolic engineering of Escherichia coli and Corynebacterium glutamicum for the -threonine
Metabolic engineering of Escherichia coli and Corynebacterium glutamicum for the -threonine

... resistance to more stringent process requirements. Obviously, directed genetic manipulations are more feasible options for subsequent isolation of mutant strains of genetically defined hyper-producers. Selection criteria would include reduced by-product formation and expanded substrate spectra. By al ...
Bacterial methionine biosynthesis
Bacterial methionine biosynthesis

... of the terminal carboxyl group (Blanco et al., 2003). The conserved biochemical logic for converting homoserine to methionine is: (i) to activate homoserine by acylating it; (ii) to effect replacement of the side chain hydroxyl group with a thiol group, giving homocysteine; and (iii) to transfer a m ...
Basic Science for Clinicians
Basic Science for Clinicians

... However, the molecular signaling role of AMP is dramatically extended by activation of the AMPK pathway. Because many more proteins are regulated by AMPK than contain specialized AMP-binding sites, the ability of AMP to signal energy compromise is greatly enhanced. AMPK is a protein kinase that has ...
(LPT1) and humans (LPCAT3)
(LPT1) and humans (LPCAT3)

... the human MBOAT5, a protein with unknown function. Cell lysates from insect cells expressing human MBOAT5 (renamed as LPCAT3) showed a 10 fold increase in LPCAT activity over control. In summary, the identification of a novel LPLAT in yeast has proved to be the founding member of LPLAT family in hum ...
Cyclic-‐di-‐AMP: another second messenger enters the fray
Cyclic-‐di-‐AMP: another second messenger enters the fray

... are   found   in   bacterial   species   but   a   small   number   are   also   present   in   archaea   within  the  Euryarchaeota  group  (Box  2)12.  In  bacteria,  DAC  domain  proteins  are   most   frequently   found   in   the   G ...
Case Study: BPTI
Case Study: BPTI

... • Type “all” in the selection box. There are a few dots outside the blue and green regions. Find out what residues they correspond to by clicking on them and explain your findings! In addition to a Ramachandran plot, a so called contact map consisting of a two dimensional plot of distances between ...
The mitochondrial carnitine/acylcarnitine carrier: Function
The mitochondrial carnitine/acylcarnitine carrier: Function

... Oxidation of fatty acids in mitochondria coupled to oxidative phosphorylation is the most important pathway for the production of metabolic energy during fasting. This process occurs in the mitochondrial matrix where the enzymes of fatty acid b-oxidation are located. Fatty acyl groups are transporte ...
LAP5 and LAP6 encode anther-specific proteins with similarity to
LAP5 and LAP6 encode anther-specific proteins with similarity to

... Several studies indicate that sporopollenin is a complex polymer composed of fatty acids and phenolic compounds (Guilford et al., 1988; Ahlers et al., 1999; Wiermann et al., 2001). Sporopollenin is remarkably strong and chemically resistant, making it difficult to determine its precise composition b ...
Genome-wide Investigation of Cellular Functions for tRNA Nucleus
Genome-wide Investigation of Cellular Functions for tRNA Nucleus

... In eukaryotic cells tRNAs are transcribed in the nucleus and exported to the cytoplasm for their essential role in protein synthesis. This export event was thought to be unidirectional. Surprisingly, several lines of evidence showed that mature cytoplasmic tRNAs shuttle between nucleus and cytoplasm ...
biolsci.org - International Journal of Biological Sciences
biolsci.org - International Journal of Biological Sciences

... KHG20612, Figure 3a) and Bacillus anthracis are documented [9, 10, 25, 26]. ...
Progesterone-stimulated intracellular calcium increase in human
Progesterone-stimulated intracellular calcium increase in human

... Dipartimento di Fisiopatologia Clinica, Unità di Andrologia, Università di Firenze, viale Pieraccini 6, I-50139 Firenze, Italy 1To ...
The C-terminal domain of the Rhizobium leguminosarum
The C-terminal domain of the Rhizobium leguminosarum

... Four PhoA fusions within the large hydrophilic loop were identified following the TnPhoA mutagenesis of pIJ1089 and pIJ1887. DNA sequencing of the fusion functions in plasmids pIJ1687, pIJ1924, pIJ1923 and pIJ1704 showed that the fusions were in frame and located after amino acids 17 (NodC17–PhoA), ...
The Genera Staphylococcus and Macrococcus
The Genera Staphylococcus and Macrococcus

... al., 2001). One potential problem for PCR is the possibility of false-negative reactions due to PCR inhibitors that might be present in some foods. Several conventional procedures for isolating S. aureus from foods are described below. Nonselective Enrichment Procedures It is often necessary to use ...
From Sequence to Structure
From Sequence to Structure

... structure (or fold) under physiological conditions. On the other hand, the demands of protein function require that the folded protein should not be too rigid. Presumably because of these constraints, the number of folds adopted by proteins, though large, is limited. Whether the limited number of fo ...
Saccharomyces cerevisiae Functional Expression and Stabilization of Horseradish Peroxidase by Directed Evolution in
Saccharomyces cerevisiae Functional Expression and Stabilization of Horseradish Peroxidase by Directed Evolution in

... (>50% inactive clones) produced no mutants with increased activity or stability against H2O2 and elevated temperature (3,300 colonies screened). Screening lower error-rate libraries produced several mutants with higher total activity (1.45–1.8-fold). Two of those (HRP H1-8H10, HRP H16E1) also retain ...
Molecular and General Genetics.
Molecular and General Genetics.

... growth primarily by colonising the root surface, causing an increase in the proliferation of the root hairs and root system of the host plant. This e€ect is thought to result from the production of auxin-like compounds, such as indole-3-acetic acid (IAA), by the bacterium, because application of IAA ...
Practical Aspects of Estimating Energy Components in
Practical Aspects of Estimating Energy Components in

... Supplemental Text 1: SNP report of the rs2403254 locus. The report contains knowledge of all genes within a 500 kbp distance of rs2403254, as retrieved from NCBI-Gene, ConsensusPathDB, UniProtKB, OMIM, Gene Ontology, TCDB, ExPASy and KEGG database. --------------------------------------------------- ...
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Expression vector

An expression vector, otherwise known as an expression construct, is usually a plasmid or virus designed for protein expression in cells. The vector is used to introduce a specific gene into a target cell, and can commandeer the cell's mechanism for protein synthesis to produce the protein encoded by the gene. Expression vectors are the basic tools in biotechnology for the production of proteins.The plasmid is engineered to contain regulatory sequences that act as enhancer and promoter regions and lead to efficient transcription of the gene carried on the expression vector. The goal of a well-designed expression vector is the production of protein, and this may be achieve by the production of significant amount of stable messenger RNA, which can then be translated into protein. The protein may be expressed constitutively, or induced when necessary using an inducer. Escherichia coli is commonly used as the host for protein expression, other cell types however may also be used. An example of the use of expression vector is the production of insulin which is used for medical treatments of diabetes.
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