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BIO 538 Molecular Mechanisms of Animal Development Name ________________________________________ Readings Worksheet 3 "Alternate RNA Splicing Regulation in the Testis" Reproduction (2006) 132: 811-819 Use other internet sources and textbooks to help you understand new terminology or concepts. 1. How would you summarize the process of RNA splicing? 2. What is the significance of alternative splicing to developmental biology? 3. What are some possible mechanisms by which the cell distinguishs between constitutive exons (those always appearing in a mRNA) and those that are alternately spliced? 4. Figure 3 shows a model for alternative splicing of the HIPK3 transcript. What are the positive-acting and negativeacting protein factors and DNA elements that affect alternative splicing?
