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Name ______________________________________ MBMB 451A Section 2 - Bartholomew Fall 2007 10-30-07 Answer the following question [14 points] 1. Describe the properties of the general transcription factors of RNA polymerase II, I and III. What is TBP and what general transcription factors have TBP as a component? What enzymatic activities do some of the transcription factors possess and how do they facilitate transcription? Describe TAFs and if they are involved in activated or basal transcription. Name ______________________________________ Answer one of the following two questions [12 points] 2. What are the principles of ion-exchange chromatography and how does it work in protein purification? What is a linear versus a step gradient? What is the difference between a weak anion exchange resin and a strong anion exchange resin? OR 3. Explain discontinuous gel electrophoresis and how it is used to separate proteins by size. What is an SDS- micelle and what is its role in gel electrophoresis? Name ______________________________________ Answer one of the following two questions [12 points] 4. Explain how DNA recombination can occur with DNA starting with a nick or with their being a double-stranded break in DNA? How does one get reciprocal versus patch recombination in this manner? OR 5. Explain how the RecBCD complex works and the role of the chi sequence? At what stage does RecA come into the process? What are the functional and structural properties of the RecA protein? Name ______________________________________ Answer one of the following two questions [14 points] 6. Describe the process of self splicing and the differences between group I and II introns. Make sure to discuss any nucleotide requirement. OR 7. Describe the process of mRNA splicing, the components of the spliceosome and how they are involved in splicing. What is a transesterification reaction and how does it relate to splicing? What are an exon and an intron, and the conserved features of the 5’ and 3’ splice junctions? Bonus question maximum of 10 points possible Describe the enzymatic properties of SWI/SNF, an ATP-dependent chromatin remodeling complex. Tell about the two models for the mechanism of ATP-dependent remodeling. Tell what is the relationship of chromatin remodeling with transcription and if there is a connection with co-activators? (write answers on the back)
