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UNIVERSITY OF OSLO
FACULTY OF MATHEMATICS AND NATURAL
SCIENCES
PAGE 1
Mid-term exam in MBV2010
Candidate nr.:
Date: March 22, 2010
This examination paper consists of 4 pages.
Appendices: none
Permitted materials: none
The number of answers (crosses) for each question must match the number shown in
brackets to the right.
1. Which of the following statements are not true?
Pyrrolysine is an amino acid
The genetic code has three stop codons
DNA has a helical diameter of 1- 3 nm
Nucleotides consist of sugar, phosphate, and a ba se
Alphoid DNA is found in telomeres
The genome of the yeast Saccharomyces cerevisiae has about 6000 genes
Eukaryotic viruses often have a head-and-tail capsid
Retroviral genomes always integrate into the genome of the host cell
Viroids contain no genes
The P element of Drosophila is a transposon
RNA transposons can be used as DNA markers
Endogenous retroviruses (ERVs) are LTR retro elements
Deoxyribose is a pentose
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2. Which of the following histones are part of a nucleosome?
Histone H1A
Histone H1B
Histone H2A
Histone H3
Histone H5
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3. Fluorescent in situ hybridization (FISH)
requires deoxynucleotides
requires a labeled probe
is used in physical mapping of genomes
is used in genetic mapping of genomes
requires a DNA polymerase
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4. Protein-protein interactions can be identified by
The yeast two-hybrid system
Microarrays
Affinity chromatography
Transposon tagging
Serial analysis of gene expression (SAGE)
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5. A STS (Sequence Tagged Site)
is a protein sequence
is an RNA sequence
is a DNA sequence
is used in genetic mapping
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UNIVERSITY OF OSLO
FACULTY OF MATHEMATICS AND NATURAL
SCIENCES
PAGE 2
Mid-term exam in MBV2010
Candidate nr.:
Date: March 22, 2010
6. A codon bias
is found in all organisms
is not found in mitochondria
is used to identify exons
is used to identify functional RNA genes
is found for all amino acids
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7. Bacterial genomes
do not contain transposons
are always circular
are compacted by supercoiling
frequently contain operons
are normally diploid
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8. An -helix
is a DNA structure
is a protein structure
winds to the left
winds to the right
is stabilized by hydrogen bonds
is stabilized by disulfide bonds
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9. Ribosomal RNAs
code for ribosomal proteins
function in transcription of genes
are the most abundant ribonucleic acids in cells
are not found in mitochondria
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10. Centromeres
contain satellite DNA
contain many tightly packed genes
are common in bacterial chromosomes
function in DNA replication
contain histones
occur only in eukaryotes
are always located in the middle of chromosomes
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11. Which of the following elements can be used as DNA marker?
Long interspersed nuclear elements (LINEs)
Long terminal repeat elements (LTR elements)
Microsatellites
Expressed sequence tags (ESTs)
Genes
DNA Transposons
Restriction fragment length polymorphisms (RFLPs)
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UNIVERSITY OF OSLO
FACULTY OF MATHEMATICS AND NATURAL
SCIENCES
PAGE 3
Mid-term exam in MBV2010
Candidate nr.:
Date: March 22, 2010
12. Genes can be inactivated by
transposon tagging
zoo-blotting
cDNA capture
homologous recombination
exon trapping
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13. DNA
is a polypeptide
contains ribose
contains guanine
is always double-stranded
is stabilized by base stacking
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14. RNA transposons
make up more than 40% of the human genome
can be as short as 100 bp
occur in prokaryotes and in eukaryotes
transpose conservatively
code for a transposase enzyme
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15. All eukaryotic nuclear chromosomes
are circular
have only one origin of replication
have only one centromer
end in telomeres
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16. Partial linkage
was discovered in the eighteenth century
was discovered by Gregor Mendel
is used in physical mapping
is only found for sequences that are on the same chromosome
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17. All template-dependent DNA polymerases
use DNA as template
synthesize DNA in 3’ -> 5’ direction
require a primer to initiate DNA synthesis
have also 5’ -> 3’ exonuclease activity
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18. The human genome
has a size of about 3.2 billion base pairs
contains less than 20,000 genes
contains more than 3 million retroelements
is divided into 25 chromosomes
is the largest genome known to date
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UNIVERSITY OF OSLO
FACULTY OF MATHEMATICS AND NATURAL
SCIENCES
PAGE 4
Mid-term exam in MBV2010
Candidate nr.:
Date: March 22, 2010
19. Reverse transcriptases
are viral enzymes
synthesize RNA
do not require a primer to function
are DNA polymerases
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20. Nucleosomes
consist of DNA and proteins
are only present in the nucleus
are found in bacteria
contain 6 proteins
are not found in telomeres
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21. -sheets are stabilized by
hydrophobic bonds
ionic bonds
hydrogen bonds
covalent bonds
all of the above
none of the above
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22. Long interspersed nuclear elements (LINEs)
are found in prokaryotes and eukaryotes
are DNA transposons
end with a poly(A) sequence
contain a gene coding for a reverse transcriptase
are usually shorter than 500 bp
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Total number of correct answers:
(43)