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Biotechnology Need To Know List This is for both chapters 13 & 14, so you better get started. You are to use the textbook, class activities and notes to know as much as possible about these concepts. Be as specific and complete as you can! Not everything here will be covered in class, nor is everything in the textbook. You must READ, PARTICIPATE, TAKE NOTES and STUDY!! All vocabulary from chapters 13 & 14 plus introduced in class! (A big part of the test!) What selective breeding produces. Examples of hybridization The ultimate source of genetic variability How breeders maintain the desired traits of an organism Why polyploidy instantly produces a new plant species How to recognize a diagram of DNA cut by a restriction enzyme What DNA analysis by gel electrophoresis allows researchers to do The technique used to make many copies of a gene What genetic engineering involves The technique of DNA sequencing How a recombinant plasmid gets inside a bacterial cell What is most often used as a genetic marker in plasmids The advantage of producing transgenic plants The technique of cloning (sheep example) The breeding practice most likely to bring together two recessive alleles How scientists produced oil-eating bacteria The function of gel electrophoresis The technique of combining DNA from different sources What knowing the sequence of an organism’s DNA allows researchers to do The technique of transformation in both bacteria and plants How a gene marker is used to distinguish transformed bacteria When transformation of a plant cell is considered successful The relationship between genetic engineering and transgenic organisms Sex determination in humans What a pedigree allows a researcher to do Inheritance of PKU, ABO blood type, sex-linked traits, Huntington’s, Tay-Sachs & Down syndrome The cause of cystic fibrosis at the genetic level How chromosomes 21 and 22 are unique Why Barr bodies are visible in only female cells The importance of the X chromosome other than determining sex The basis for DNA fingerprinting How the human genome was sequenced The technique of gene therapy The cause of sickle cell disease The technique of DNA probing Interpret diagrams of gel electrophoresis, cloning, a pedigree, and a karyotype