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Biology 12 Unit D - DNA DNA Workshop Go to my website at http://sd67.bc.ca/teachers/barcuri/ → Click on Biology 12 → course outline → Unit D – DNA → DNA workshop assignment. Open this assignment and use it to follow the links. This will make it easier than typing the links in yourself. Your welcome! Go to the following websites and answer the questions below. http://caligula.bcs.deakin.edu.au/bcs_courses/forensic/Chemical%20Detective/DNA_Struct ure.htm The weight of DNA can range from __________ amu to _________________ amu. What are the 3 basic units that make up a nucleotide? What does the phosphate group look like? Draw and name the four bases. Show all of the elements in each base. Biology 12 Unit D - DNA Which bases have double rings? _______________ and ________________ The single strands of DNA are wound together to form a ________________, the two strands being held together by attractions, a called _____________ bonds, between the bases attached to the _____________ - _______________ backbone. Give the names of the bases that always match with the base given below. Adenine: ______________ Cytosine: ___________________ Click on the link to The Function of DNA DNA stores an organism's __________________ information and controls the production of ___________________ and is thus responsible for the _____________________ of an organism. Where is DNA normally found? ________________________ What are chromosomes? __________________________________________ The sections of chromosomes are called ___________ Protein is a polymer of amino acids. If one wrong amino acids is placed in the wrong order a mutation cam occur. What condition occurs if the wrong amino acid is substituted in haemoglobin? ______________________ What is the base sequence for this condition? ___________ - _________ - _________ Click on the link RFLP DNA Typing What are the 4 parts to this procedure? Biology 12 Unit D - DNA Second Website: http://www.ucmp.berkeley.edu/glossary/gloss3/dna.html If an entire molecule of DNA from a virus was the scale shown, the image would be ______________ high. For the bacteria, E.Coli, the image would be ___________________ long, and for a typical piece of DNA from a eukaryote cell, the image would stretch for ________________________ . How many times larger is the DNA in the eukaryote cell compared to the DNA in the virus cell. ( You may need to do some math!) Why do you think that it is so much longer? (Hint: What is the function of DNA exactly) What force holds the 2 DNA strands together? _______________ What is the benefit to having hydrogen bonds, in regards to replication? Click on the word BACTERIA in the second paragraph. What are 3 importance uses of bacteria? Click on the word VIRUS in the second paragraph. What does a virus do when it comes into contact with a host cell? What is the dormant phase of the viruses life called? _______________ Biology 12 Unit D - DNA List 8 diseases that a virus can cause. Third Website: http://www.pbs.org/wgbh/aso/tryit/dna/# Read the Intro page Click on: Go directly to DNA Workshop Activity Click on DNA Replication – complete the reaction Click on DNA replication Once complete go back to the homepage and click on DNA Replication List the 3 steps to DNA replication 1. 2. 3. Only one mistake may exist in every __________ base pairs. That's the same as typing out the entire Encyclopaedia Britannica five times and typing in a wrong letter only once! At the bottom of this web page, click on All Wound Up The DNA is wrapped around spools of a protein called _____________ . Fourth Website: http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/D/DNAReplication.html What does the term semi-conservative imply? Biology 12 Unit D - DNA The single molecule of DNA in the bacteria, E. coli contains 4.7 x 106 nucleotide pairs. DNA replication begins at a single, fixed location in this molecule, called the replication origin, it proceeds at about _______ nucleotides per second, and thus is done in approximately _____ minutes. The average human chromosome contains 150 x 106 nucleotide pairs, which are copied at about 50 base pairs per second. How long does it take to replicate? ________ . Why is it so much faster? Last but not least. http://www.pbs.org/wgbh/nova/sheppard/analyze.html This web site will allow you to use Gel electrophoresis to determine the sequence of some DNA and help you solve a crime through DNA finger printing. Read the intro page Go to Part I – Read Go to Part II - Read If it says start over! You messed up and you must restart.