Download Figure 13-1

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Name: __________________________________ Per.: ______ Date: _________
Ch. 13 Genetic Engineering
Multiple Choice: Select the choice that best completes the statement or answers the
question.
Figure 13-1
1. What does Figure 13-1 show?
a. Gel electrophoresis
b. DNA sequencing
c. A restriction enzyme producing a DNA fragment
d. Polymerase chain reaction
Figure 13-1
2. Which statements are TRUE about structures C and D? (Select more than one)
a. They are sticky ends
b. They are made by different restriction enzymes.
c. They will attach to DNA fragments cut with the same enzyme.
d. They are genetic markers.
3. Combining genes from different sources into a single DNA molecule is known as:
a. DNA fingerprinting
c. PCR
b. cloning
d. Recombinant DNA technoloy
4.
a.
b.
c.
d.
What is an advantage of producing transgenic plants?
Increasing herbicide resistance.
Using more pesticides.
Producing clones.
Studying human genes.
5.
a.
b.
c.
d.
Which of the following steps is NOT essential in producing recombinant DNA?
Cut out a piece of DNA from a DNA molecule.
Splice a piece of DNA into DNA from another organism.
Use a restriction enzyme to form sticky ends in DNA.
Separating gene fragments of different lengths by gel electrophoresis.
6.
a.
b.
c.
d.
A stretch of DNA that varies among individuals is called a(n):
clone
Resistant gene
antibiotic
Genetic marker
7.
a.
b.
c.
d.
What kind of cell (or cells) are used to make animal clones?
Body cell only
Egg cell only
Egg cell and sperm cell
Body cell and egg cell
8. On an electrophoresis gel, band B is closer to the positive end of the gel than is band
A. Which of the following statements is true?
a. Band B is more negatively charged than band A.
b. Band B moved faster than band A.
c. Band A is smaller than band B.
d. Band B consists of larger DNA fragments than does band A.
9.
a.
b.
c.
d.
10.
a.
b.
c.
d.
Analyzing DNA by gel electrophoresis allows researchers to …
Compare DNA samples from different sources.
Determine whether a particular allele of a gene is dominant or recessive.
Compare the phenotypes of different organisms.
Cut DNA with restriction enzymes.
One function of gel electrophoresis is to:
Separate DNA fragments.
Cut DNA
Recombine DNA
Extract DNA
11. Which process is used to make copies of DNA fragments from small samples (like a
drop of blood)?
a. Gel electrophoresis
b. Recombinant DNA technology
c. Therapeutic cloning
d. Polymerase chain reaction
e. Reproductive cloning
12. What is an advantage of using genetically engineered bacteria to produce human
proteins?
a. The human proteins produced by genetically engineered bacteria work better than
those produced by humans.
b. Genetically engineered bacteria can mass-produce pure human proteins.
c. The human proteins produced by genetically engineered bacteria last longer than
those produced by humans.
d. Genetically engineered bacteria can produce human proteins to make plastics.
13.
a.
b.
c.
d.
Stem cells are valuable to genetic research scientists because …
They multiply at very fast rates.
They are resistant to mutations.
They have the ability to specialize and become many types of cells.
They contain a different gene sequence than specialized cells of the body.
14. Therapeutic cloning can use stem cells from the following sources for potential
cures: (Circle all that apply).
a. embryoes
b. Nerve cells (neurons)
c. Umbilical cord blood
d. Bone marrow
e. Sperm and egg cells
15.
a.
b.
c.
d.
Which choice is NOT used by bacteria to turn on or off gene expression?
Operator
Repressor protein
Promoter
Restriction enzymes
16.
a.
b.
c.
d.
When a repressor protein is bound to a operator …
Genes are translated.
Genes are not transcribed.
Genes are duplicated.
Genes are deleted.
True or False? Correct the false statement. A = TRUE; B = False
17. ___________________ Bacteria can share antibiotic resistance by exchanging
plasmids.
18. ___________________ Red blood cells and skin cells contain the same genes.
19. ___________________ Nucleic acid probes are used to cut DNA into fragments.
True or False? Correct the false statement. A = TRUE; B = False
20. ___________________ In bacteria, a promoter is cluster of related genes plus its
control sequences to turn on or off transcription.
21. ___________________ A protein produced by a transgenic bacteria is different
from the same protein produced by humans.
23. ___________________ Stomach cells and nerve cells express the same genes.
22. ___________________ Ligase helps attach sticky ends together.
23. ___________________ Therapeutic cloning and stem cells are commonly used to
treat diseases.
24. ___________________ Plasmids are small loops of bacterial DNA.
25. ___________________ Selective breeding involves direct manipulation of DNA.
26. ___________________ Epigenetics looks at how environmental factors affects
gene expression.
Open Ended
1. List and describe three applications of biotechnology being used today. Be
specific. Provide examples that DIFFERENT from one another (agriculture,
industry, or medicine)
2. What is therapeutic cloning? What are the potential benefits? What is the
concern or controversy surrounding this process?