* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Download struktur dan fungsi kromosom
Epigenetics of neurodegenerative diseases wikipedia , lookup
DNA polymerase wikipedia , lookup
Bisulfite sequencing wikipedia , lookup
Site-specific recombinase technology wikipedia , lookup
Skewed X-inactivation wikipedia , lookup
No-SCAR (Scarless Cas9 Assisted Recombineering) Genome Editing wikipedia , lookup
Human genome wikipedia , lookup
Epigenetics wikipedia , lookup
Gel electrophoresis of nucleic acids wikipedia , lookup
DNA damage theory of aging wikipedia , lookup
Nutriepigenomics wikipedia , lookup
Designer baby wikipedia , lookup
United Kingdom National DNA Database wikipedia , lookup
Epigenetics in learning and memory wikipedia , lookup
Genomic library wikipedia , lookup
Histone acetyltransferase wikipedia , lookup
Genome (book) wikipedia , lookup
Molecular cloning wikipedia , lookup
Genealogical DNA test wikipedia , lookup
Comparative genomic hybridization wikipedia , lookup
DNA vaccination wikipedia , lookup
Primary transcript wikipedia , lookup
Nucleic acid analogue wikipedia , lookup
Cancer epigenetics wikipedia , lookup
Point mutation wikipedia , lookup
Vectors in gene therapy wikipedia , lookup
Cell-free fetal DNA wikipedia , lookup
Epigenetics of human development wikipedia , lookup
Nucleic acid double helix wikipedia , lookup
Cre-Lox recombination wikipedia , lookup
Non-coding DNA wikipedia , lookup
Deoxyribozyme wikipedia , lookup
Therapeutic gene modulation wikipedia , lookup
Microevolution wikipedia , lookup
Y chromosome wikipedia , lookup
History of genetic engineering wikipedia , lookup
Extrachromosomal DNA wikipedia , lookup
Epigenomics wikipedia , lookup
Helitron (biology) wikipedia , lookup
DNA supercoil wikipedia , lookup
X-inactivation wikipedia , lookup
Polycomb Group Proteins and Cancer wikipedia , lookup
STRUKTUR DAN FUNGSI KROMOSOM Niken Satuti Nur Handayani Lab. Genetika Fakultas Biologi UGM Genetics: From Genes to Genomes Second Edition Hartwell ● Hood ● Goldberg ● Reynolds ● Silver ● Veres The bacterial genome is composed of one circular chromosome 4-5 Mb long Condenses by supercoiling and looping into a densely packed nucleoid body Chromosomes replicate inside cell and cell divides by binary fission Fig. 14.4 b Genetics: From Genes to Genomes Second Edition Hartwell ● Hood ● Goldberg ● Reynolds ● Silver ● Veres The Eukaryotic Chromosome An Organelle for Packaging and managing DNA Each chromosome packages a single long molecule of DNA Chemical and Physical analysis Studies examine stretching and recoiling find longer pieces recoil more slowly than shorter pieces Pulse field electrophoresis Separates large pieces of DNA – number and sizes correspond to number and sizes expected if each chromosome contains a single piece of DNA Protein components of DNA Histone proteins abound the chromatin of all eukaryotic cells Histones – small proteins with basic, positively charged amino acids lysine and arginine Bind to and neutralize negatively charged DNA Make up half of all chromatin protein by weight Five types: H1, H2A, H2B, H3, and H4 Core histones make up nucleosome: H2A, H2B, H3, and H4 DNA and histone synthesis regulation correlate timing so both are synthesized together High level of similarity of histones among diverse organisms Protein components of DNA Nonhistone proteins are a heterogeneous group Half of proteins in chromatin are nonhistone Large variety of nonhistone proteins – 200 – 2,000,000 in diploid genomes Large variety of functions Scaffold – backbone of chromosome DNA replications – e.g., DNA polymerases Chromosome segregation – e.g., motor proteins of kinetichores Transcriptional regulation – largest group regulates transcription during gene expression Occur in different amounts in different tissues because of variety of function The nucleosome: the fundamental unit of chromosomal packaging arises from DNAs association with histones Chromatin fibers with beads having diameter of about 100 A and strings having diameter of 20 A The nucleosome: the fundamental unit of chromosomal packaging arises from DNAs association with histones Bead is a nucleosome with about 160 bp of DNA wrapped twice around a core of 8 histones 40 bp of DNA link together nucleosomes The nucleosome: the fundamental unit of chromosomal packaging arises from DNAs association with histones X-ray diffraction analysis DNA does not coil smoothly Base sequences dictate preferred nucleosome positions along DNA Spacing and structure affect genetic function The nucleosome: the fundamental unit of chromosomal packaging arises from DNAs association with histones Spacing of nucleosomes affects gene expression Regions between nucleosomes available for interactions with proteins involved in expression, regulation, and further compaction Determines how and whether certain proteins interact with specific sequences Packaging into nucleosomes condenses DNA sevenfold 2 meters of DNA shortens to less than 0.25 meters Models of higher level compaction seek to explain extreme compaction of chromosomes at mitosis Formation of 300 A fiber through supercoiling Models of higher level compaction seek to explain extreme compaction of chromosomes at mitosis Radial loop-scaffold model for higher levels of compaction Each loop contains 60-100 kb of DNA tethered by nonhistone scaffold proteins Radial loop-scaffold model continued Experimental support for radial loopscaffold model Electron micrograph shows long DNA loops emanating from the protein scaffold A closer look at karyotypes: fully compacted metaphase chromosomes have unique, reproducible banding patterns Banding patterns are highly reproducible Not known what they represent A closer look at karyotypes: fully compacted metaphase chromosomes have unique, reproducible banding patterns. Banding patterns help locate genes A closer look at karyotypes: fully compacted metaphase chromosomes have unique, reproducible banding patterns Banding patterns can be used to analyze chromosomal differences between species Can also be used to reveal cause of genetic disease e.g., Downs syndrome – 3 copies of chromosome 21 Centromere structure and function Fig. 12.11 a Kromosom Eukaryotik dan Prokaryotik 1. 2. AUTOSOM : kromosom yang tidak ada hubungannya dengan penentuan jenis kelamin KROMOSOM KELAMIN (sex chromosome) : kromosom yang ada hubungannya dengan penentuan jenis kelamin Chromosome structure HISTONES are proteins with a high proportion of Positively charged amino acids (lysine and arginin), Which enable them to bind firmly to the negatively Charged DNA doble helix. There are 5 types of histone molecules: H1, H2A, H2B, H3, and H4. Except for H1, they occur in exactly equal numbers. In less condensed chromatin fibers, it becomes apparent that DNA between nucleosomes is bound to H1 histones. DNA in metaphase chromosomes must be shortened 10,000-fold by very efficient packing. This occurs in discrete units, the NUCLEOSOMES, Consisting of DNA and histones. Chromosome Each chromosome has a constriction point called the centromere, which divides the chromosome into two sections, or arms. The short arm of the chromosome is labeled the p arm. The long arm of the chromosome is labeled the q arm. Characteristic shapes of chromosomes Short arm Long arm The location of the centromere on each chromosome gives the chromosome its characteristic shape, and can be used to help describe the location of specific genes. BENTUK KROMOSOM Ada empat bentuk kromosom berdasarkan letak sentromer: 1. 2. TELOSENTRIS : sentromer terletak di terminal (bagian ujung kromosom) AKROSENTRIS/SUB TELOSENTRIS : sentromer terletak di sub terminal (mendekati ujung kromosom) 3. SUB METASENTRIS : sentromer terletak di sub median (mendekati bagian tengah) 4. METASENTRIS : sentromer terletak di median (bagian tengah) 1 2 3 4 Nilai Indeks Sentromer Bentuk kromosom Nilai Indeks Sentromer (NIS) p x 100 p+q p = panjang lengan pendek kromosom q = panjang lengan panjang kromosom p+q = panjang absolut kromosom Nilai Indeks Sentromer Posisi Sentromer Bentuk Kromosom Simbol Kromosom Rata-rata NIS Median Metasentris m 38 -50 Sub Median Sub Metasentris sm 26 - 37 Sub Terminal Akrosentris / Sub Telosentris st 13 - 25 Terminal Telosentris t 0 - 12 Karyotype A display of the paired homologues chromosomes from a cell Allows determination of: sex of an individual, abnormal chromosome number, other chromosome abnormalities, etc. How many chromosomes do people have? Banding pattern during metaphase Banding pattern during prophase In humans, each cell normally contains 23 pairs of chromosomes, for a total of 46. The 22 autosomes are numbered by size. The other two chromosomes, X and Y, are the sex chromosomes.