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GENE REGULATION Definition • The mechanisms that determine the types of cells in which a gene will be transcribed. • When the mRNA will be exported to the cytoplasm. • When and how often the mRNA will be translated, • and the duration of time before the mRNA is degraded. Regulation categories • Negative regulation • Positive regulation • Negative regulation is more common in prokaryotes, positive regulation in eukaryotes. Negative regulation • Means that the default state of transcription is (ON) unless a repressor turns it (OFF). Repressible transcription • Repressible transcription needs a corepressor to bind with the aporepressor to make an active repressor. Transcription wont occur. Inducible transcription • Inducible transcription needs an inducer to bind with the repressor and inactivate it, so the repressor loses its capability to bind DNA. Transcription will occur. Positive regulation • Means that the default state of transcription is (OFF), unless a protein turns transcription ON, which is called transcriptional activator protein. It binds the activator binding site on DNA. Autoregulation • Means that the protein product of a gene regulates its own transcription. • Most genes include positive and negative regulatory elements. Constitutive synthesis • Transcription and translation of a gene takes place at a constant rate independent of the presence or absence of any inducer or repressor molecules. Operator • It is a regulatory region (or binding site) in DNA for the repressor protein to control gene transcription. Operon • A collection of genes regulated by an operator and repressor. Promoter • A DNA sequence at which RNA polymerase binds and initiate transcription. • RNA polymerase is an enzyme responsible for transcription of DNA to RNA. TATA box • The base sequence 5’-TATA-3’ in the DNA of a promoter. • TATA-box-binding protein: A protein that binds to the TATA motif in the promoter region of a gene. Not only ON-OFF system Ex:(Tryptophan biosynthesis) • trpR gene produces trp aporepressor (regulatory protein) . It does not bind to the operator unless it is first bound to tryptophan. That means botn form trp repressor. • Insufficient tryptophan means that aporepressor wont bind trp operator and transcription occurs. • Trytophan acts as co-repressor. Transcriptional activator proteins • Helix-turn-helix motif • DNA-binding motif. Enhancers • Enhancers are found at a variety of locations around the gene they regulate. • Short (less than 20 base pairs) Silencers • Short sequences of DNA. • They promote the assembly of large protein complexes that prevent transcription.