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From Gene to Protein Chapter 17 OVERVIEW • DNA RNA Protein • Transcription –Synthesis of mRNA from DNA • Translation (change of language) –Synthesis of protein (polypeptide) from mRNA (uses tRNA) Figure 17.2 Overview: the roles of transcription and translation in the flow of genetic information Figure 17.3 The triplet code CODONS • Three base nucleotides that eventually code for a specific amino acid • There are 64 codons • Marshall Nirenberg (1961) deciphered first codon –Found UUU codes for phenylalanine • Amino acids abbreviated by first 3 letters of name or designated single letter • AUG = methionine (met) or start • UAA, UAG, and UGA = stop codons • Nearly universal language among all living organisms Figure 17.4 The dictionary of the genetic code RNA vs. DNA • Ribose instead of deoxyribose • Uracil instead of thymine • Single strand vs. double strand TRANSCRIPTION Making pre-mRNA INITIATION • Promoter – site where RNA polymerase attaches to DNA and starts transcription • Transcription factors – proteins that mediate the binding of RNA polymerase (in eukaryotes – huge role in gene regulation) • TATA box – sequence of nucleotides (TATAAAA) that is part of promoter region and binds to transcription factors • RNA polymerase attaches to promoter, helix unwinds, and elongation begins Figure 17.6 The stages of transcription: initiation, elongation, and termination Figure 17.6 The stages of transcription: initiation, elongation, and termination Figure 17.7 The initiation of transcription at a eukaryotic promoter ELONGATION • RNA polymerase adds complementary nucleotides in 5’ to 3’ direction • About 60 nucleotides per second Figure 17.6 The stages of transcription: initiation, elongation, and termination TERMINATION • Elongation stops at or just following a stop codon Figure 17.6 The stages of transcription: initiation, elongation, and termination RNA PROCESSING • In eukaryotes… • The 5’ end of pre-mRNA is capped with guanine • Poly(A) tail – several adenine added to 3’ end – Protects end – Signal for future ribosome attachment – Help to get mRNA out of nucleus – Help prevent degradation Figure 17.8 RNA processing; addition of the 5 cap and poly(A) tail RNA SPLICING • In eukaryotes… • Large portions of mRNA do not code for parts of a protein • Introns – noncoding segments • Exons – coding segments • snRNPs (small nuclear ribonucleoproteins) combine with proteins to make spliceosome • Spliceosomes cut at ends of introns and rejoins remaining exons together (recognize special sequences) • Ribozymes – mRNA that catalyzes its own intron removal (not all enzymes are proteins) Figure 17.9 RNA processing: RNA splicing Figure 17.10 The roles of snRNPs and spliceosomes in mRNA splicing WHY INTRONS? • Split genes can code for different proteins or different regions of same polypeptide • Introns increase the cross over frequency between 2 alleles which increases diversity Figure 17.11 Correspondence between exons and protein domains TRANSLATION mRNA to protein Figure 17.12 Translation: the basic concept tRNA • Shorter than mRNA • Shaped like an “L” • A specific amino acid attaches to 3’ end • Loop region contains anticodon Figure 17.13a The structure of transfer RNA (tRNA) Figure 17.13b The structure of transfer RNA (tRNA) • Aminoacyl-tRNA synthetases - bind correct amino acid to a tRNA –There are 20 of these synthetases so each amino acid has its own enzyme –Driven by hydrolysis of ATP Figure 17.14 An aminoacyl-tRNA synthetase joins a specific amino acid to a tRNA RIBSOMES • Made of ribosomal RNA (rRNA) and protein • Two subunits: large and small – Join only when translation occurring • Three binding sites for tRNA – E = exit site • tRNA leaves ribosome – P = peptidyl-tRNA binding site • Holds growing polypeptide – A = Aminoacyl-tRNA binding site • Holds next tRNA with next amino acid Figure 17.15 The anatomy of a functioning ribosome Figure 17.16 Structure of the large ribosomal subunit at the atomic level INITIATION • Small ribosomal subunit binds to mRNA at 5’end. • Initiator tRNA (with met) binds to P site (H bonds between anitcodon and codon). • Large ribosomal subunit attaches with help of proteins • GTP supplies energy. Figure 17.17 The initiation of translation ELONGATION • H bonds between codon and anticodon (in A site)connect next tRNA with next amino acid • GTP needed. • A ribozyme catalyzes peptide bond between first and second amino acid. • Peptide attached to second tRNA. • mRNA moves through ribosome so that the first tRNA leaves via E site and second tRNA moves to P site. • Then the third tRNA comes in to A site. • Movement requires GTP. • Process continues like a conveyer belt. Figure 17.18 The elongation cycle of translation • Wobble effect – third base of anticodon can pair with noncomplementary base of codon (a U can bind to a A or G) • Inosine (I) can bond with U, C, or A TERMINATION • When a stop codon reaches the A site there is no matching anticodon on a tRNA. • Release factor protein binds instead. • Polypeptide released by hydrolysis. • Ribsome disassembles. Figure 17.19 The termination of translation Misc. • Polyribosomes - several ribosomes can translate the same mRNA strand • All synthesis of all proteins begins in cytoplasm • Signal peptide sends protein to ER • Signal peptide is recognized by signal recognition particle (SRP) • Proteins are transorted via rough ER and can be modified in Golgi body (ex. Removal of first met) Figure 17.20 Polyribosomes Figure 17.21 The signal mechanism for targeting proteins to the ER Prokaryote vs. Eukaryote • Prokaryotes – No introns or TATA box – No 5’ G cap or poly A tail – Translation begins before mRNA is completely made (remember no nucleus) • Eukaryotes – Introns and TATA box – Cap and tail (protection for exiting nucleus) – mRNA must leave nucleus before translation can start Figure 17.22 Coupled transcription and translation in bacteria MUTATIONS • Mutation – a change in DNA sequence • Point Mutations cause: – missense mutations no change in amino acid(s) – nonsense mutations changes amino acid and therefore protein • Two types of Point Mutations – Base pair substitutions replacement of nucleotide – Insertions and Deletions -additions or losses of one or more nucleotides • Frameshift mutation - occurs when number of nucleotides inserted or deleted is not 3 or a multiple of 3 • Mutation rate is ~1 nucleotide altered in every 1010 Figure 17.23 The molecular basis of sickle-cell disease: a point mutation Figure 17.24 Categories and consequences of point mutations: Base-pair insertion or deletion Figure 17.24 Categories and consequences of point mutations: Base-pair substitution MUTAGENS • Physical or chemical agents cause DNA to mutate –X-rays –UV light –Radiation –Most carcinogens A gene is more than just a protein maker. • A gene is a region of DNA whose final product is protein or RNA • Types of RNA made include – mRNA, tRNA, rRNA, snRNA, SRP RNA (part of signal recognition particle), snoRNA (small nucleolar RNA helps process prerRNA), and siRNA (small interfering RNA) and miRNA (micro RNA) both involved in gene regulation Figure 17.25 A summary of transcription and translation in a eukaryotic cell