Understanding The Function And Regulation Of Eukaryotic Release
... understanding of the mechanism of stop codon recognition utilized by eRF1, since eRF1 3-dimensitional structure is significantly different. To gain a better understanding of how eRF1 is able to mediate this essential function, we extensively analyzed two motifs known to play a significant role in st ...
... understanding of the mechanism of stop codon recognition utilized by eRF1, since eRF1 3-dimensitional structure is significantly different. To gain a better understanding of how eRF1 is able to mediate this essential function, we extensively analyzed two motifs known to play a significant role in st ...
ATP– and ADP–DnaA protein, a molecular switch in gene regulation
... did not bind to the isolated DnaA box 2, we conclude that both forms of DnaA protein bind in a cooperative way to the oligonucleotide including DnaA boxes 1 and 2. However, the DNase I footprint results (Figure 1), as well as the lower stoichiometry found in BIAcore experiments, show that ADP–DnaA i ...
... did not bind to the isolated DnaA box 2, we conclude that both forms of DnaA protein bind in a cooperative way to the oligonucleotide including DnaA boxes 1 and 2. However, the DNase I footprint results (Figure 1), as well as the lower stoichiometry found in BIAcore experiments, show that ADP–DnaA i ...
Posttranslational Modification of MDM2
... COOH terminus of p53 (23), the identities of the autoubiquitination sites in MDM2 have not been published. The balance between auto- and substrate-ubiquitination of MDM2 is modulated physiologically by posttranslational modifications, including sumoylation and phosphorylation. On SUMO conjugation to ...
... COOH terminus of p53 (23), the identities of the autoubiquitination sites in MDM2 have not been published. The balance between auto- and substrate-ubiquitination of MDM2 is modulated physiologically by posttranslational modifications, including sumoylation and phosphorylation. On SUMO conjugation to ...
Pathways of NeurSospora crassa and Saccharomyces cerevisiaet
... mM for the N. crassa enzyme. They are also high for the S. (ereviisiae enzyme (258), although specific values have not been reported. The unusually high pH optimum and high substrate requirements of the enzyme may be related to its location within the small volume of the mitochondria, where it is lo ...
... mM for the N. crassa enzyme. They are also high for the S. (ereviisiae enzyme (258), although specific values have not been reported. The unusually high pH optimum and high substrate requirements of the enzyme may be related to its location within the small volume of the mitochondria, where it is lo ...
Plakoglobin domains that define its association with the
... intermediate filament cytoskeleton. A short stretch of 37 amino acids in the C terminus of desmocollin-1 was recently shown by Troyanovsky et al. (1994a) to be necessary for it to associate with plakoglobin and to recruit intermediate filaments to the plasma membrane. Using similar experiments, they ...
... intermediate filament cytoskeleton. A short stretch of 37 amino acids in the C terminus of desmocollin-1 was recently shown by Troyanovsky et al. (1994a) to be necessary for it to associate with plakoglobin and to recruit intermediate filaments to the plasma membrane. Using similar experiments, they ...
BAG1, a negative regulator of Hsp70 chaperone activity, uncouples
... Permanently denatured [125I]RCMLA or unfolded β-gal was incubated with either Hsp70, or Hsp70 and BAG-1 in the presence or absence of ATP. In the absence of ATP, Hsp70 forms a stable complex with [125I]RCMLA, as detected in the native gel assay (Figure 2, lane 2), that rapidly dissociates upon addit ...
... Permanently denatured [125I]RCMLA or unfolded β-gal was incubated with either Hsp70, or Hsp70 and BAG-1 in the presence or absence of ATP. In the absence of ATP, Hsp70 forms a stable complex with [125I]RCMLA, as detected in the native gel assay (Figure 2, lane 2), that rapidly dissociates upon addit ...
Affinity Precipitation of a Monoclonal Antibody ELP-Z Stimuli Responsive Biopolymer
... acid residues that selectively binds to the Fc portion of IgGs with high affinity (KD ¼ 10–50 nM) (Braisted and Wells, 1996). The helices are in close contact with one another, forming a hydrophobic core that provides a great deal of stability to this domain (Linhult et al., 2004). In addition to the ...
... acid residues that selectively binds to the Fc portion of IgGs with high affinity (KD ¼ 10–50 nM) (Braisted and Wells, 1996). The helices are in close contact with one another, forming a hydrophobic core that provides a great deal of stability to this domain (Linhult et al., 2004). In addition to the ...
With No Lysine (WNK) Family Proteins and Their
... in β strand 3 conserved in all other functional kinase domains, the WNKs contain a cysteine. An alternate lysine in β strand 2 functions in its place. The four mammalian genes of the WNK family code for long proteins, 1200 to 2400 amino acids in length. However, outside of the kinase domain, there a ...
... in β strand 3 conserved in all other functional kinase domains, the WNKs contain a cysteine. An alternate lysine in β strand 2 functions in its place. The four mammalian genes of the WNK family code for long proteins, 1200 to 2400 amino acids in length. However, outside of the kinase domain, there a ...
New insights into regulation of p53 protein degradation
... Calpains are a family of calcium-dependent intracellular proteases that can be divided into two major groups: the ubiquitous calpains, and tissue-specific calpains [51]. As calpains activity can be regulated by autoproteolysis and the inhibitor protein calpastatin, suggesting that, like the proteaso ...
... Calpains are a family of calcium-dependent intracellular proteases that can be divided into two major groups: the ubiquitous calpains, and tissue-specific calpains [51]. As calpains activity can be regulated by autoproteolysis and the inhibitor protein calpastatin, suggesting that, like the proteaso ...
2014_ACIC - DIGITAL.CSIC, el repositorio institucional
... 2.1 Gemini surfactants with amino acids or peptides as headgroups One approach to minimize the toxicity of cationic bisQuats surfactants was to design soft Gemini molecules from biocompatible single chain amino acid-based surfactants. These new molecules combine the advantages of Gemini surfactants ...
... 2.1 Gemini surfactants with amino acids or peptides as headgroups One approach to minimize the toxicity of cationic bisQuats surfactants was to design soft Gemini molecules from biocompatible single chain amino acid-based surfactants. These new molecules combine the advantages of Gemini surfactants ...
Diacylglycerol kinase zeta in hypothalamus interacts with long form leptin receptor. Relation to dietary fat and body weight regulation
... This report focuses on DGK, which belongs to a subgroup of DGKs that has unknown physiological function (10 –14). DGK is characterized by its four C-terminal ankyrin repeats and a unique region homologous to MARCKS phosphorylation site domain. DGK exists in both the cytosol and nucleus under the ...
... This report focuses on DGK, which belongs to a subgroup of DGKs that has unknown physiological function (10 –14). DGK is characterized by its four C-terminal ankyrin repeats and a unique region homologous to MARCKS phosphorylation site domain. DGK exists in both the cytosol and nucleus under the ...
Collagen in the Human Lung
... Collagen is an integral component of lung. It maintains alveolar, airway, and vascular stability, limits lung expansion, and contributes significantly to lung recoil at all lung volumes (1, 2). In the adult, the total amount of collagen and the concentration of collagen per unit lung mass remain con ...
... Collagen is an integral component of lung. It maintains alveolar, airway, and vascular stability, limits lung expansion, and contributes significantly to lung recoil at all lung volumes (1, 2). In the adult, the total amount of collagen and the concentration of collagen per unit lung mass remain con ...
Substrate specificity and inhibitors of LRRK2, a protein kinase
... protein kinase inhibitors staurosporine (IC50 2 nM), K252 (IC50 4 nM) and Su-11248/sunitinib (IC50 15 nM) [7,8], no selective LRRK2 inhibitors have been reported thus far. We previously undertook a KESTREL (kinase substrate tracking and elucidation) screen in rat brain extracts to identify proteins ...
... protein kinase inhibitors staurosporine (IC50 2 nM), K252 (IC50 4 nM) and Su-11248/sunitinib (IC50 15 nM) [7,8], no selective LRRK2 inhibitors have been reported thus far. We previously undertook a KESTREL (kinase substrate tracking and elucidation) screen in rat brain extracts to identify proteins ...
Toxoplasma gondii Chitinase Induces Macrophage Activation
... have been described (Table 1), some of their characteristics were elucidated herein: (a) the optimal pH for chitinase activity is usually in the range of 4.0–7.0; (b) the optimal temperature is usually 50°C; and (c) their molecular mass is commonly between 15 and 50 kDa. Our research on a chitinase ...
... have been described (Table 1), some of their characteristics were elucidated herein: (a) the optimal pH for chitinase activity is usually in the range of 4.0–7.0; (b) the optimal temperature is usually 50°C; and (c) their molecular mass is commonly between 15 and 50 kDa. Our research on a chitinase ...
Identification of a C-terminal Poly(A)-binding Protein (PABP)
... Pessoal de Nı́vel Superior, and the British Council. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. ** To whom correspo ...
... Pessoal de Nı́vel Superior, and the British Council. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. ** To whom correspo ...
Alternative mRNA Splicing Generates the Two
... isolated by hybridization to pRCAl.9. The entire protein coding region of the spinach rubisco activase gene, shown in Figure 4, was found to reside on two EcoRl fragments of approximately 1.8 kb and 2.1 kb in length. The genomic clones were completely sequenced and, by comparison with cDNA sequences ...
... isolated by hybridization to pRCAl.9. The entire protein coding region of the spinach rubisco activase gene, shown in Figure 4, was found to reside on two EcoRl fragments of approximately 1.8 kb and 2.1 kb in length. The genomic clones were completely sequenced and, by comparison with cDNA sequences ...
Ribosomal Stalk Protein L12: Structure, Function and
... The ribosome is composed of ribosomal proteins (r-proteins) and ribosomal RNAs (rRNAs) arranged in two subunits, the large subunit is about twice the size of the small subunit. The ribosomes, ribosomal subunits and rRNAs are called by their sedimentation coefficient (S). The intact ribosome is calle ...
... The ribosome is composed of ribosomal proteins (r-proteins) and ribosomal RNAs (rRNAs) arranged in two subunits, the large subunit is about twice the size of the small subunit. The ribosomes, ribosomal subunits and rRNAs are called by their sedimentation coefficient (S). The intact ribosome is calle ...
Prions as protein-based genetic elements. - Lindquist Lab
... the prion determinant is transient (24, 176). Thus, [PSI+] and [URE3] are selfperpetuating, even after the levels of Sup35 and Ure2 return to normal. Once established, most prions propagate faithfully through mitosis and meiosis and are rarely spontaneously lost, although there are some exceptions ( ...
... the prion determinant is transient (24, 176). Thus, [PSI+] and [URE3] are selfperpetuating, even after the levels of Sup35 and Ure2 return to normal. Once established, most prions propagate faithfully through mitosis and meiosis and are rarely spontaneously lost, although there are some exceptions ( ...
Purification and Characterization of Extracellular and
... 300 ml. This concentrate was filtered through a further ultrafiltration membrane (Amicon-PM 10). The final volume was about 10 ml and corresponded to a 2000-fold concentration of the killer toxin. Pur8cation of the killer toxin by ion-exchange chromatography. The concentrated and desalted supernatan ...
... 300 ml. This concentrate was filtered through a further ultrafiltration membrane (Amicon-PM 10). The final volume was about 10 ml and corresponded to a 2000-fold concentration of the killer toxin. Pur8cation of the killer toxin by ion-exchange chromatography. The concentrated and desalted supernatan ...
Misleading and deceptive use of "No MSG" to hide
... continued proliferating. At the USDA they don't simply fail to enforce the regulation. The USDA actually approves labels of meat and poultry products that claim "No MSG," "No MSG Added," or "No Added MSG" when they contain free glutamic acid. Clearly, it is false and misleading to claim "No MSG" or ...
... continued proliferating. At the USDA they don't simply fail to enforce the regulation. The USDA actually approves labels of meat and poultry products that claim "No MSG," "No MSG Added," or "No Added MSG" when they contain free glutamic acid. Clearly, it is false and misleading to claim "No MSG" or ...
Identification and analysis of new phloem proteins from
... sorting inside the SE (van Bel and Knoblauch, 2000). The SE plastids are classified into two types. The S-type plastids contain only starch inclusions while P-type plastids enclose protein and starch bodies. The proposed role of the SE plastids is the involvement in the wound-induced occlusion of si ...
... sorting inside the SE (van Bel and Knoblauch, 2000). The SE plastids are classified into two types. The S-type plastids contain only starch inclusions while P-type plastids enclose protein and starch bodies. The proposed role of the SE plastids is the involvement in the wound-induced occlusion of si ...
Protein (nutrient)
Proteins are essential nutrients for the human body. They are one of the building blocks of body tissue, and can also serve as a fuel source. As a fuel, proteins contain 4 kcal per gram, just like carbohydrates and unlike lipids, which contain 9 kcal per gram. The most important aspect and defining characteristic of protein from a nutritional standpoint is its amino acid composition.Proteins are polymer chains made of amino acids linked together by peptide bonds. During human digestion, proteins are broken down in the stomach to smaller polypeptide chains via hydrochloric acid and protease actions. This is crucial for the synthesis of the essential amino acids that cannot be biosynthesized by the body.There are nine essential amino acids which humans must obtain from their diet in order to prevent protein-energy malnutrition. They are phenylalanine, valine, threonine, tryptophan, methionine, leucine, isoleucine, lysine, and histidine. There are five dispensable amino acids which humans are able to synthesize in the body. These five are alanine, aspartic acid, asparagine, glutamic acid and serine. There are six conditionally essential amino acids whose synthesis can be limited under special pathophysiological conditions, such as prematurity in the infant or individuals in severe catabolic distress. These six are arginine, cysteine, glycine, glutamine, proline and tyrosine.Humans need the essential amino acids in certain ratios. Some protein sources contain amino acids in a more or less 'complete' sense. This has given rise to various ranking systems for protein sources, as described in the article.Animal sources of protein include meats, dairy products, fish and eggs. Vegan sources of protein include whole grains, pulses, legumes, soy, and nuts. Vegetarians and vegans can get enough essential amino acids by eating a variety of plant proteins. It is commonly believed that athletes should consume a higher-than-normal protein intake to maintain optimal physical performance.