![Cloning vectors for the expression of green fluorescent protein](http://s1.studyres.com/store/data/000004138_1-be6b7602f94ffb92f3be527ae3b197ed-300x300.png)
Accumulation of xylem transported protein at pit membranes and
... material. Primary walls are based on cellulose fibre matrices interspersed with up to 800 different types of cell wall proteins (Albenne et al., 2009). The xylem pit membranes are traversed by tortuous pores with mean diameters at around 5 nm, as estimated by perfusion experiments with gold nano par ...
... material. Primary walls are based on cellulose fibre matrices interspersed with up to 800 different types of cell wall proteins (Albenne et al., 2009). The xylem pit membranes are traversed by tortuous pores with mean diameters at around 5 nm, as estimated by perfusion experiments with gold nano par ...
Lipid modulation of skeletal muscle mass and function
... work will be required to determine which of these DAG molecules, if any, are responsible for conveying muscle wasting actions. In an attempt to address this, future studies may involve treating cultured muscle cells with different DAG molecules/stereoisomers in order to determine their effects on my ...
... work will be required to determine which of these DAG molecules, if any, are responsible for conveying muscle wasting actions. In an attempt to address this, future studies may involve treating cultured muscle cells with different DAG molecules/stereoisomers in order to determine their effects on my ...
LNBI 9043 - Lupin Allergy: Uncovering Structural Features and
... The two major lupin storage proteins are α-conglutin (legumin-like or 11S globulin), and ß-conglutin (vicilin-like or 7S globulin). Vilicin proteins are characterized by two cupin (barrel-shaped) domains constituted by α-helices. Another family with a cupin-like structure, γ-conglutin (basic 7S-glob ...
... The two major lupin storage proteins are α-conglutin (legumin-like or 11S globulin), and ß-conglutin (vicilin-like or 7S globulin). Vilicin proteins are characterized by two cupin (barrel-shaped) domains constituted by α-helices. Another family with a cupin-like structure, γ-conglutin (basic 7S-glob ...
Characterization of the Cytosolic Proteins Involved in the Amoeboid
... actin, these cells power their movement through modulation of the major sperm protein (MSP) cytoskeleton. MSP forms dense filament meshworks that pack the sperm lamellipod. Protrusion is associated with the assembly of MSP filaments at the leading edge of the lamellipod, and retraction is connected ...
... actin, these cells power their movement through modulation of the major sperm protein (MSP) cytoskeleton. MSP forms dense filament meshworks that pack the sperm lamellipod. Protrusion is associated with the assembly of MSP filaments at the leading edge of the lamellipod, and retraction is connected ...
A B23-interacting sequence as a tool to visualize protein interactions
... contrary to other nucleolar proteins such as Nopp140, nucleolin, the other major nucleolar protein, and Nop52. The interaction between B23 and the NoLS is direct, as demonstrated by GST-NoLS pull-down assays performed on purified B23 (Fig. 2B). To determine the region within B23 required for B23-NoL ...
... contrary to other nucleolar proteins such as Nopp140, nucleolin, the other major nucleolar protein, and Nop52. The interaction between B23 and the NoLS is direct, as demonstrated by GST-NoLS pull-down assays performed on purified B23 (Fig. 2B). To determine the region within B23 required for B23-NoL ...
Characterising Non-Structural Protein NS4 of African Horse Sickness Virus
... [5]. There are nine known serotypes of AHSV [6,7]. The AHSV genome consists of ten doublestranded RNA segments [8]. As in the case of BTV, it has been widely accepted for decades that each of the ten AHSV genome segments (Seg-1 to Seg-10) contains a single open reading frame (ORF), thereby encoding ...
... [5]. There are nine known serotypes of AHSV [6,7]. The AHSV genome consists of ten doublestranded RNA segments [8]. As in the case of BTV, it has been widely accepted for decades that each of the ten AHSV genome segments (Seg-1 to Seg-10) contains a single open reading frame (ORF), thereby encoding ...
Novel Ciliate Genetic Code Variants Including the Reassignment of
... densities of each stop codon (see Methods section for the description of the pipeline). Blepharisma and Paramecium were used as reference organisms for determining a threshold for discrimination between stop codons that were reassigned to code for amino acids and stop codons that function as signals ...
... densities of each stop codon (see Methods section for the description of the pipeline). Blepharisma and Paramecium were used as reference organisms for determining a threshold for discrimination between stop codons that were reassigned to code for amino acids and stop codons that function as signals ...
Ubiquitination and Auxin Signaling: A Degrading Story
... which auxin is involved tell of the daunting complexity behind the plant’s response to auxin. This one molecule can cause changes in rates of cell division and cell elongation, alter ion fluxes across membranes, cue changes in patterning and differentiation, and affect the expression of hundreds of ...
... which auxin is involved tell of the daunting complexity behind the plant’s response to auxin. This one molecule can cause changes in rates of cell division and cell elongation, alter ion fluxes across membranes, cue changes in patterning and differentiation, and affect the expression of hundreds of ...
A novel exon within the mdm2 gene modulates translation
... and Vousden, 1998). Indeed, there is evidence that mdm2 directly functions as a ubiquitin ligase for p53 (Honda et al., 1997; Honda and Yasuda, 1999). Nuclear-cytoplasmic shuttling by mdm2 may also be important in accelerating the degradation of p53 (Roth et al., 1998). These various properties of m ...
... and Vousden, 1998). Indeed, there is evidence that mdm2 directly functions as a ubiquitin ligase for p53 (Honda et al., 1997; Honda and Yasuda, 1999). Nuclear-cytoplasmic shuttling by mdm2 may also be important in accelerating the degradation of p53 (Roth et al., 1998). These various properties of m ...
The Shwachman-Diamond SBDS protein localizes to the nucleolus
... contains deletions and nucleotide changes that prevent the generation of a functional protein.9 The majority of patients (nearly 75%) with Shwachman-Diamond syndrome were found to harbor SBDS mutations arising from a gene conversion event with this pseudogene.9 The SBDS gene is predicted to encode a ...
... contains deletions and nucleotide changes that prevent the generation of a functional protein.9 The majority of patients (nearly 75%) with Shwachman-Diamond syndrome were found to harbor SBDS mutations arising from a gene conversion event with this pseudogene.9 The SBDS gene is predicted to encode a ...
Review: Derivatization in mass spectrometry—6. Formation of mixed
... A further application of this approach is the investigation of organic binders used by artists. The method allows a fast and effective identification of amino acids in very small amounts of protein by ion trap GC/MS after hydrolysis of proteinic binders (such as casein, albumin, gelatine, etc.) and ...
... A further application of this approach is the investigation of organic binders used by artists. The method allows a fast and effective identification of amino acids in very small amounts of protein by ion trap GC/MS after hydrolysis of proteinic binders (such as casein, albumin, gelatine, etc.) and ...
original version
... membrane [68]. However, another hypothesis yet to be addressed is whether accumulation of UPLP may also adversely affect oriC-dependent DNA initiation. DnaA protein initiates chromosomal replication at oriC once per cell cycle. A third mechanism to suppress the growth arrest of acidic phospholipid-d ...
... membrane [68]. However, another hypothesis yet to be addressed is whether accumulation of UPLP may also adversely affect oriC-dependent DNA initiation. DnaA protein initiates chromosomal replication at oriC once per cell cycle. A third mechanism to suppress the growth arrest of acidic phospholipid-d ...
The Multiple Personalities of the Regulatory Subunit of Protein
... proliferation and survival [3, 4]. Genetic studies in organisms such as yeast and slime mould have revealed that CK2 is essential for viability [5, 6]. Recent studies of molecular clock machinery in Drosophila and Arabidopsis have also provided evidence for the involvement of CK2 in circadian oscill ...
... proliferation and survival [3, 4]. Genetic studies in organisms such as yeast and slime mould have revealed that CK2 is essential for viability [5, 6]. Recent studies of molecular clock machinery in Drosophila and Arabidopsis have also provided evidence for the involvement of CK2 in circadian oscill ...
Rodolfo GhirlandoƗ, Radina Mutskova¥, Chad
... Ferritin is an iron-storage protein distributed in high concentrations in the liver and spleen but also found in the heart and kidney. Ferritins from all species have 24 protein subunits arranged in 4,3,2 symmetry to form a spherical and hollow complex with an approximately 8 nm diameter cavity capa ...
... Ferritin is an iron-storage protein distributed in high concentrations in the liver and spleen but also found in the heart and kidney. Ferritins from all species have 24 protein subunits arranged in 4,3,2 symmetry to form a spherical and hollow complex with an approximately 8 nm diameter cavity capa ...
Full-Text PDF
... membrane [68]. However, another hypothesis yet to be addressed is whether accumulation of UPLP may also adversely affect oriC-dependent DNA initiation. DnaA protein initiates chromosomal replication at oriC once per cell cycle. A third mechanism to suppress the growth arrest of acidic phospholipid-d ...
... membrane [68]. However, another hypothesis yet to be addressed is whether accumulation of UPLP may also adversely affect oriC-dependent DNA initiation. DnaA protein initiates chromosomal replication at oriC once per cell cycle. A third mechanism to suppress the growth arrest of acidic phospholipid-d ...
Resistance of Helicoverpa armigera to Cry1Ac toxin from Bacillus
... the possibility of an emergence of resistance against Cry1Ac due to failure of implementing the recommended cultivation regimen have been raised, thus questioning the sustained use of these transgenics [3,4]. Laboratory-reared populations of H. armigera showing resistance to Cry1Ac have been reporte ...
... the possibility of an emergence of resistance against Cry1Ac due to failure of implementing the recommended cultivation regimen have been raised, thus questioning the sustained use of these transgenics [3,4]. Laboratory-reared populations of H. armigera showing resistance to Cry1Ac have been reporte ...
Full Article - PDF - Journal of Biotech Research
... neurotransmitter synthesis. P. falciparum is capable of synthesizing folate de novo or obtaining it through salvage, whereas humans can only obtain folate from their diet, making the folate pathway products important targets for chemotherapy. Although P. falciparum folate pathway enzymes and metabol ...
... neurotransmitter synthesis. P. falciparum is capable of synthesizing folate de novo or obtaining it through salvage, whereas humans can only obtain folate from their diet, making the folate pathway products important targets for chemotherapy. Although P. falciparum folate pathway enzymes and metabol ...
Chapter 5 The Structure and Function of Large Biological Molecules
... 22) All of the following statements concerning saturated fats are true except A) They are more common in animals than in plants. B) They have multiple double bonds in the carbon chains of their fatty acids. C) They generally solidify at room temperature. D) They contain more hydrogen than saturated ...
... 22) All of the following statements concerning saturated fats are true except A) They are more common in animals than in plants. B) They have multiple double bonds in the carbon chains of their fatty acids. C) They generally solidify at room temperature. D) They contain more hydrogen than saturated ...
Comparative Analysis of ,Multiple Protein
... call this a semiconserved motif (e.g., see fig. 3, motif 11). Occasionally a single residue, which is completely conserved among all members of a protein family, is found between larger motifs. In such cases we consider the single residue as one of the motifs comprising the ordered series of motifs ...
... call this a semiconserved motif (e.g., see fig. 3, motif 11). Occasionally a single residue, which is completely conserved among all members of a protein family, is found between larger motifs. In such cases we consider the single residue as one of the motifs comprising the ordered series of motifs ...
Protein (nutrient)
Proteins are essential nutrients for the human body. They are one of the building blocks of body tissue, and can also serve as a fuel source. As a fuel, proteins contain 4 kcal per gram, just like carbohydrates and unlike lipids, which contain 9 kcal per gram. The most important aspect and defining characteristic of protein from a nutritional standpoint is its amino acid composition.Proteins are polymer chains made of amino acids linked together by peptide bonds. During human digestion, proteins are broken down in the stomach to smaller polypeptide chains via hydrochloric acid and protease actions. This is crucial for the synthesis of the essential amino acids that cannot be biosynthesized by the body.There are nine essential amino acids which humans must obtain from their diet in order to prevent protein-energy malnutrition. They are phenylalanine, valine, threonine, tryptophan, methionine, leucine, isoleucine, lysine, and histidine. There are five dispensable amino acids which humans are able to synthesize in the body. These five are alanine, aspartic acid, asparagine, glutamic acid and serine. There are six conditionally essential amino acids whose synthesis can be limited under special pathophysiological conditions, such as prematurity in the infant or individuals in severe catabolic distress. These six are arginine, cysteine, glycine, glutamine, proline and tyrosine.Humans need the essential amino acids in certain ratios. Some protein sources contain amino acids in a more or less 'complete' sense. This has given rise to various ranking systems for protein sources, as described in the article.Animal sources of protein include meats, dairy products, fish and eggs. Vegan sources of protein include whole grains, pulses, legumes, soy, and nuts. Vegetarians and vegans can get enough essential amino acids by eating a variety of plant proteins. It is commonly believed that athletes should consume a higher-than-normal protein intake to maintain optimal physical performance.