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Purification, Cloning, and Functional Characterization of a Novel
... Cinnamomun kanehirai Hay is a well-known and highly valued medicinal bitter mushroom in Taiwan and other Oriental countries, but is virtually unknown in the Western world. It has become popular as a folkloric medicine as well as a source of physiologically beneficial mushrooms in Taiwan and has been ...
... Cinnamomun kanehirai Hay is a well-known and highly valued medicinal bitter mushroom in Taiwan and other Oriental countries, but is virtually unknown in the Western world. It has become popular as a folkloric medicine as well as a source of physiologically beneficial mushrooms in Taiwan and has been ...
PDF - Walter Lab
... (Figure 2, lanes 4 and 5). In particular, three additional bands of higher molecular weight were visible that correspond to the addition of one, two, or three core-oligosaccharide moieties, and are labeled paF.1, paF.2, and paF.3. Increasing the membrane concentration caused a shift toward the fully ...
... (Figure 2, lanes 4 and 5). In particular, three additional bands of higher molecular weight were visible that correspond to the addition of one, two, or three core-oligosaccharide moieties, and are labeled paF.1, paF.2, and paF.3. Increasing the membrane concentration caused a shift toward the fully ...
Preparation of Lentiviral expression construct DNA
... accompanying Product Datasheet. If it is proven to the satisfaction of GeneCopoeia that the Product fails to meet these specifications, GeneCopoeia will replace the Product. In the event a replacement cannot be provided, GeneCopoeia will provide the purchaser with a refund. This limited warranty sha ...
... accompanying Product Datasheet. If it is proven to the satisfaction of GeneCopoeia that the Product fails to meet these specifications, GeneCopoeia will replace the Product. In the event a replacement cannot be provided, GeneCopoeia will provide the purchaser with a refund. This limited warranty sha ...
Ageing and the aggregating proteoglycans of
... proteolytic activity capable of degrading proteoglycans. Similar enzymes are produced by a variety of connective tissue cells from many species [78]. Such proteoglycan-degrading enzymes have broad pH profiles, being optimally active around neutrality, and require Ca2+for activity. They are produced ...
... proteolytic activity capable of degrading proteoglycans. Similar enzymes are produced by a variety of connective tissue cells from many species [78]. Such proteoglycan-degrading enzymes have broad pH profiles, being optimally active around neutrality, and require Ca2+for activity. They are produced ...
Snake venomics of Bothrops punctatus
... 150 µL, after removal of growth medium (DMEM with 10% FCS). Controls for 0 and 100% toxicity consisted of assay medium, and 0.1% Triton X-100 diluted in assay medium, respectively. After 3 h at 37◦ C, a supernatant aliquot was collected to determine the lactic dehydrogenase (LDH; EC 1.1.1.27) activi ...
... 150 µL, after removal of growth medium (DMEM with 10% FCS). Controls for 0 and 100% toxicity consisted of assay medium, and 0.1% Triton X-100 diluted in assay medium, respectively. After 3 h at 37◦ C, a supernatant aliquot was collected to determine the lactic dehydrogenase (LDH; EC 1.1.1.27) activi ...
fulltekst
... All complex organisms, like an animal or a plant, are built up of small specified cells forming different tissues that together create the organism. Within its cell nucleus, each cell contains identical genomic material that describes the construction of different proteins performing different funct ...
... All complex organisms, like an animal or a plant, are built up of small specified cells forming different tissues that together create the organism. Within its cell nucleus, each cell contains identical genomic material that describes the construction of different proteins performing different funct ...
mTORC1 and the regulation of skeletal muscle anabolism and mass
... the mammalian target of rapamycin complex 1 (mTORC1) is a critical signalling complex that regulates muscle mass. In response to nutrition and resistance exercise, increased muscle mass and activation of mTORC1 occur in parallel. In this review, we summarize recent findings on mTORC1 and its regulat ...
... the mammalian target of rapamycin complex 1 (mTORC1) is a critical signalling complex that regulates muscle mass. In response to nutrition and resistance exercise, increased muscle mass and activation of mTORC1 occur in parallel. In this review, we summarize recent findings on mTORC1 and its regulat ...
Protein phosphorylation in chloroplasts – a survey of
... available in PhosphAT, 27 published papers as well as unpublished in-house data from the Schulze laboratory were integrated into one dataset. With this combination, a surprisingly high rate of tyrosine phosphorylation in the entire dataset and in chloroplast proteins was reported, even though the da ...
... available in PhosphAT, 27 published papers as well as unpublished in-house data from the Schulze laboratory were integrated into one dataset. With this combination, a surprisingly high rate of tyrosine phosphorylation in the entire dataset and in chloroplast proteins was reported, even though the da ...
Molecular Clocks
... • This method has been applied to the entire protein databank and the deamidation rates of all Asn in 30,000 proteins are listed on the internet at www.deamidation.org. ...
... • This method has been applied to the entire protein databank and the deamidation rates of all Asn in 30,000 proteins are listed on the internet at www.deamidation.org. ...
Turnover of protein phosphorylation evolving under
... i.e., they have evolved only recently by directional selection and tests of conservation among species reject the hypothesis that they are under evolutionary constraint. While this hypothesis is of biological interest, there is currently very little data supporting this possibility (but see Jensen e ...
... i.e., they have evolved only recently by directional selection and tests of conservation among species reject the hypothesis that they are under evolutionary constraint. While this hypothesis is of biological interest, there is currently very little data supporting this possibility (but see Jensen e ...
Holding it all together? Candidate proteins for the plant Golgi matrix
... plant Golgi are the GRASP stacking proteins, whose interaction with the golgins is largely responsible for the organisation of mammalian Golgi. Again, this might reflect the differences in the organisation of these two types of Golgi. The plant genome might contain additional, plant-specific, golgin ...
... plant Golgi are the GRASP stacking proteins, whose interaction with the golgins is largely responsible for the organisation of mammalian Golgi. Again, this might reflect the differences in the organisation of these two types of Golgi. The plant genome might contain additional, plant-specific, golgin ...
ENS’06 FUSION PHAGE AS A BIOSELECTIVE NANOMATERIAL: EVOLUTION OF THE CONCEPT
... chemical, and structural properties. During infection of the host Escherichia coli, the phage coat is dissolved in the bacterial cytoplasmic membrane, while viral DNA enters the cytoplasm [12]. The protein is synthesized in infected cell as a water-soluble precursor, which contains a leader sequence ...
... chemical, and structural properties. During infection of the host Escherichia coli, the phage coat is dissolved in the bacterial cytoplasmic membrane, while viral DNA enters the cytoplasm [12]. The protein is synthesized in infected cell as a water-soluble precursor, which contains a leader sequence ...
2. Solid-phase peptide synthesis (SPPS) - RSC Publishing
... 1). In particular, the posttranslational modifications are essential for protein activation: phosphorylation for signal transduction, ubiquitination for proteolysis, attachment of fatty acids for membrane anchoring or glycosylation for extending protein half-life, targeting, and cell-cell interactio ...
... 1). In particular, the posttranslational modifications are essential for protein activation: phosphorylation for signal transduction, ubiquitination for proteolysis, attachment of fatty acids for membrane anchoring or glycosylation for extending protein half-life, targeting, and cell-cell interactio ...
A defined subset of adenylyl cyclases is regulated by bicarbonate ion.
... determine whether HCO3 - up regulated cyaB1595-859 specific activity by increasing homodimer formation we examined the ratio of the HCO3- and Cl- specific activities as a function of protein concentration. Interestingly, this ratio remained constant over the range of protein concentrations tested in ...
... determine whether HCO3 - up regulated cyaB1595-859 specific activity by increasing homodimer formation we examined the ratio of the HCO3- and Cl- specific activities as a function of protein concentration. Interestingly, this ratio remained constant over the range of protein concentrations tested in ...
The SWISS-PROT protein sequence data bank
... DR (Data bank Reference) lines that point to EMBL, PDB, OMIM and PROSITE. In this particular example it is therefore possible to retrieve the nucleic acid sequence(s) that encodes for that protein (EMBL), the description of genetic disease(s) associated with that protein (OMIM), the 3D structure (PD ...
... DR (Data bank Reference) lines that point to EMBL, PDB, OMIM and PROSITE. In this particular example it is therefore possible to retrieve the nucleic acid sequence(s) that encodes for that protein (EMBL), the description of genetic disease(s) associated with that protein (OMIM), the 3D structure (PD ...
Deep mutational scanning reveals tail anchor
... that mutations recovered using our Gal4p-based scheme are unlikely to be related to ...
... that mutations recovered using our Gal4p-based scheme are unlikely to be related to ...
Essays41 Chap03 - Essays in Biochemistry
... easily cover this distance by diffusion, and by use of these substrates it has been found that archaebacterial proteasomes preferentially hydrolyse peptide bonds at the C-terminal side of large hydrophobic amino-acid residues, thus exhibiting chymotrypsin-like specificity. However, within protein su ...
... easily cover this distance by diffusion, and by use of these substrates it has been found that archaebacterial proteasomes preferentially hydrolyse peptide bonds at the C-terminal side of large hydrophobic amino-acid residues, thus exhibiting chymotrypsin-like specificity. However, within protein su ...
proposal-aug25
... specific examples to experimentally test the mechanisms of function of the identified disordered regions. This proposal represents a new approach to attack a difficult problem in protein biochemistry: the function of intrinsically disordered proteins. II. Background and Motivation As many as 50% of ...
... specific examples to experimentally test the mechanisms of function of the identified disordered regions. This proposal represents a new approach to attack a difficult problem in protein biochemistry: the function of intrinsically disordered proteins. II. Background and Motivation As many as 50% of ...
Sugar Signaling and Proteolysis in Higher Plants
... The role played by the enzymes of Suc breakdown (invertase, sucrose synthase) and trehalose metabolism (trehalose-P synthase, trehalose-P phosphatase, trehalase) in the sugar regulation of growth and development has also been studied (Eastmond and Graham 2003; Koch 2004). Thus, the expression of ye ...
... The role played by the enzymes of Suc breakdown (invertase, sucrose synthase) and trehalose metabolism (trehalose-P synthase, trehalose-P phosphatase, trehalase) in the sugar regulation of growth and development has also been studied (Eastmond and Graham 2003; Koch 2004). Thus, the expression of ye ...
Glycerolipid transfer for the building of membranes in plant cells.
... can hydrolyze acyl-ACP and release free fatty acids. Free fatty acids released from the inner envelope are channelled to the outer envelope, possibly via the At1g54350 ABC transporter and reactivated to acyl-CoA in the outer envelope [35]. Xu et al. identified another chloroplastic ABC type protein ...
... can hydrolyze acyl-ACP and release free fatty acids. Free fatty acids released from the inner envelope are channelled to the outer envelope, possibly via the At1g54350 ABC transporter and reactivated to acyl-CoA in the outer envelope [35]. Xu et al. identified another chloroplastic ABC type protein ...
Modular evolution of phosphorylation
... carbohydrate, mRNA and phosphoinositides [15,16]. One means by which phosphorylation can modify a protein’s function is to induce a conformational change (allostery), which in the case of enzymes can lead to altered catalytic activity. Protein kinases themselves provide intensely studied examples of ...
... carbohydrate, mRNA and phosphoinositides [15,16]. One means by which phosphorylation can modify a protein’s function is to induce a conformational change (allostery), which in the case of enzymes can lead to altered catalytic activity. Protein kinases themselves provide intensely studied examples of ...
A Raman spectroscopic study of the interaction between nucleotides
... acids, the composition and sequence of which are known.536717The very large amount of acidic amino acids (about 25-30% is present as aspartic and glutamic acid residues) results in an isoelectric point a t pH 5.6 Among the other residues are 18 phenylalanines, 5 tryptophans, and 8 tyrosines. The pre ...
... acids, the composition and sequence of which are known.536717The very large amount of acidic amino acids (about 25-30% is present as aspartic and glutamic acid residues) results in an isoelectric point a t pH 5.6 Among the other residues are 18 phenylalanines, 5 tryptophans, and 8 tyrosines. The pre ...
attachment of amino acids to tRNA
... cycles: In cells, the small and large ribosome subunits associate with each other and the mRNA, translate it, and then dissociate after each round of translation. This sequence of association and dissociation is called the ribosome cycle. ...
... cycles: In cells, the small and large ribosome subunits associate with each other and the mRNA, translate it, and then dissociate after each round of translation. This sequence of association and dissociation is called the ribosome cycle. ...
Protein (nutrient)
Proteins are essential nutrients for the human body. They are one of the building blocks of body tissue, and can also serve as a fuel source. As a fuel, proteins contain 4 kcal per gram, just like carbohydrates and unlike lipids, which contain 9 kcal per gram. The most important aspect and defining characteristic of protein from a nutritional standpoint is its amino acid composition.Proteins are polymer chains made of amino acids linked together by peptide bonds. During human digestion, proteins are broken down in the stomach to smaller polypeptide chains via hydrochloric acid and protease actions. This is crucial for the synthesis of the essential amino acids that cannot be biosynthesized by the body.There are nine essential amino acids which humans must obtain from their diet in order to prevent protein-energy malnutrition. They are phenylalanine, valine, threonine, tryptophan, methionine, leucine, isoleucine, lysine, and histidine. There are five dispensable amino acids which humans are able to synthesize in the body. These five are alanine, aspartic acid, asparagine, glutamic acid and serine. There are six conditionally essential amino acids whose synthesis can be limited under special pathophysiological conditions, such as prematurity in the infant or individuals in severe catabolic distress. These six are arginine, cysteine, glycine, glutamine, proline and tyrosine.Humans need the essential amino acids in certain ratios. Some protein sources contain amino acids in a more or less 'complete' sense. This has given rise to various ranking systems for protein sources, as described in the article.Animal sources of protein include meats, dairy products, fish and eggs. Vegan sources of protein include whole grains, pulses, legumes, soy, and nuts. Vegetarians and vegans can get enough essential amino acids by eating a variety of plant proteins. It is commonly believed that athletes should consume a higher-than-normal protein intake to maintain optimal physical performance.