Chapter 4 Calsequestrin - Department of Molecular Physiology and
... able to draw Ca 2+ away from the sarcoplasmic Ca 2+ -binding proteins which have Kd values for Ca2+ binding of about 1-5 µM (Greaser et al., 1972b; Kretsinger. 1976). The contest for Ca2+ is, therefore, unequal and Ca 2+ finds itself in the lumen of the sarcoplasmic reticulum There it encounters ano ...
... able to draw Ca 2+ away from the sarcoplasmic Ca 2+ -binding proteins which have Kd values for Ca2+ binding of about 1-5 µM (Greaser et al., 1972b; Kretsinger. 1976). The contest for Ca2+ is, therefore, unequal and Ca 2+ finds itself in the lumen of the sarcoplasmic reticulum There it encounters ano ...
Dissociation of a ll0-kD Peripheral Membrane Protein from the Golgi
... He Golgi apparatus is the central structure within the cell through which newly synthesized secretory and membrane proteins pass, become modified, and are sorted en route to their final destination inside or outside of the cell. The striking morphology of this organdie, consisting of ordered stacks ...
... He Golgi apparatus is the central structure within the cell through which newly synthesized secretory and membrane proteins pass, become modified, and are sorted en route to their final destination inside or outside of the cell. The striking morphology of this organdie, consisting of ordered stacks ...
COP9 signalosome turns the key on protein degradation
... the regulation of early seedling development by light. The COP1 and CSN null phenotypes would be expected to resemble each other if COP1 were the first strictly CSN-dependent E3 ligase to be active during plant development. Third, the CSN is related evolutionarily and structurally to the proteasome ...
... the regulation of early seedling development by light. The COP1 and CSN null phenotypes would be expected to resemble each other if COP1 were the first strictly CSN-dependent E3 ligase to be active during plant development. Third, the CSN is related evolutionarily and structurally to the proteasome ...
Ethan Frome - Pistachio Health Institute
... plant-based proteins, including beans and nuts, props for being nutrient-rich choices that may help improve Americans’ diets. The Guidelines suggest that there are health advantages of eating a diet that is rich in fruits and vegetables, whole grains, beans, nuts and seeds, because they’re naturally ...
... plant-based proteins, including beans and nuts, props for being nutrient-rich choices that may help improve Americans’ diets. The Guidelines suggest that there are health advantages of eating a diet that is rich in fruits and vegetables, whole grains, beans, nuts and seeds, because they’re naturally ...
The Case of Protein Kinase CK2
... The catalytic subunits of CK2α and CK2α’ are the products of separate genes located in different chromosomes. The 330 N-terminal amino acids exhibit over 90% sequence identity. However, the C-terminal sequences are unrelated (Olsten & Litchfield, 2004). The unique Cterminal domains of the catalytic ...
... The catalytic subunits of CK2α and CK2α’ are the products of separate genes located in different chromosomes. The 330 N-terminal amino acids exhibit over 90% sequence identity. However, the C-terminal sequences are unrelated (Olsten & Litchfield, 2004). The unique Cterminal domains of the catalytic ...
Photopolymerized Cross-Linked Polyacrylamide Gels for On
... channels (i.e., regions near a T junction). Voids or “tears” formed near the offset double-T junction upon application of an electric field if this extended photopolymerization step was omitted. The tears may arise from stresses induced by electroosmotic flow in channels filled with a nonuniformly p ...
... channels (i.e., regions near a T junction). Voids or “tears” formed near the offset double-T junction upon application of an electric field if this extended photopolymerization step was omitted. The tears may arise from stresses induced by electroosmotic flow in channels filled with a nonuniformly p ...
Isolation and Characterization of a Novel Ribosome
... protein, termed PAP-H, was purified from Agrobacterium rhizogenes-transformed hairy roots of pokeweed (Phytolacca americana). The protein was purified by anion- and cation-exchange chromatography. PAP-H has a molecular mass of 29.5 kD as detected by sodium dodecyl sulfate-polyacrylamide gel electrop ...
... protein, termed PAP-H, was purified from Agrobacterium rhizogenes-transformed hairy roots of pokeweed (Phytolacca americana). The protein was purified by anion- and cation-exchange chromatography. PAP-H has a molecular mass of 29.5 kD as detected by sodium dodecyl sulfate-polyacrylamide gel electrop ...
Use of Organic Acids as a Model to Study the Impact of
... gut epithelial secretions and by enterocyte attrition and shedding. Up to 50% of the ileal nitrogen is of endogenous origin, and reduced microbial competition for it has been shown to improve nitrogen retention in pigs fed formic acid [29]. The higher luminal pH in the lower gut would appear to favo ...
... gut epithelial secretions and by enterocyte attrition and shedding. Up to 50% of the ileal nitrogen is of endogenous origin, and reduced microbial competition for it has been shown to improve nitrogen retention in pigs fed formic acid [29]. The higher luminal pH in the lower gut would appear to favo ...
Enzyme from Banana (Musa sp.) Extraction Procedures for Sensitive
... In the cleaning step, there has been hydrosoluble and liposoluble enzyme separation. The former were precipitated, centrifuged and separated from the liposolubles, which were discarded along with the organic medium. Consequently, there was a decrease in total protein concentration, from 27% up to 72 ...
... In the cleaning step, there has been hydrosoluble and liposoluble enzyme separation. The former were precipitated, centrifuged and separated from the liposolubles, which were discarded along with the organic medium. Consequently, there was a decrease in total protein concentration, from 27% up to 72 ...
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... and amniotic fluids and foetal blood were collected and tested for the presence of heterologous proteins by means of the interfacial ring test and in some cases immunoelectrophoresis. Subsequent dehydration, clearing and embedding of the membranes was carried out according to Nairn (1964). After dew ...
... and amniotic fluids and foetal blood were collected and tested for the presence of heterologous proteins by means of the interfacial ring test and in some cases immunoelectrophoresis. Subsequent dehydration, clearing and embedding of the membranes was carried out according to Nairn (1964). After dew ...
The MKK7 gene encodes a group of c-Jun NH2
... These interactions between Rho GTPases and MAPKKKs may contribute to the effects of Rho GTPases on JNK activation. MAPKKK protein kinases that do not interact with Rho GTPases may mediate the effects of Rho-independent signals that lead to JNK activation. Several MAPKKs have been identified. ERK is ...
... These interactions between Rho GTPases and MAPKKKs may contribute to the effects of Rho GTPases on JNK activation. MAPKKK protein kinases that do not interact with Rho GTPases may mediate the effects of Rho-independent signals that lead to JNK activation. Several MAPKKs have been identified. ERK is ...
Two decades of studying non-covalent biomolecular assemblies by
... be probed. Multiple activation approaches have been developed to achieve this, including by impacting a surface [51,52], interactions with electrons [53,54] and absorption of infrared photons [55]. All of these approaches have their advantages, but collision-induced dissociation (CID) remains the mo ...
... be probed. Multiple activation approaches have been developed to achieve this, including by impacting a surface [51,52], interactions with electrons [53,54] and absorption of infrared photons [55]. All of these approaches have their advantages, but collision-induced dissociation (CID) remains the mo ...
Proteomic Approach to Identify Novel
... 25% in primary metabolism such as pyruvate decarboxylation, citric acid cycle, and amino acid and nucleotide metabolism. Five identified proteins represent chaperones; others are involved in molecular transport or protection against O2. Only two identified proteins form part of the mitochondrial pro ...
... 25% in primary metabolism such as pyruvate decarboxylation, citric acid cycle, and amino acid and nucleotide metabolism. Five identified proteins represent chaperones; others are involved in molecular transport or protection against O2. Only two identified proteins form part of the mitochondrial pro ...
eIF-3 - Universidad Autónoma de San Luis Potosí
... translation of a eukaryotic mRNA is the methylated cap that marks the 5’ end. Messengers whose caps have been removed are not translated efficiently. Binding of 40S subunits to mRNA requires several initiation factors, including proteins that recognize the structure of the cap. ...
... translation of a eukaryotic mRNA is the methylated cap that marks the 5’ end. Messengers whose caps have been removed are not translated efficiently. Binding of 40S subunits to mRNA requires several initiation factors, including proteins that recognize the structure of the cap. ...
Protein transfer of Agrobacterium tumefaciens Vir proteins to
... on the presence of a secretion channel, the type IV secretion system (T4SS), spanning the bacterial envelope. This pore or pilus structure is made up of eleven different VirB proteins and the coupling protein VirD4, which is thought to recruit effector proteins for translocation (Baron et al, 2002; ...
... on the presence of a secretion channel, the type IV secretion system (T4SS), spanning the bacterial envelope. This pore or pilus structure is made up of eleven different VirB proteins and the coupling protein VirD4, which is thought to recruit effector proteins for translocation (Baron et al, 2002; ...
PeptidePicker: a Tool for Determining Most Appropriate Peptides for
... an analysis pipeline that organizes, generates, and verifies transition lists, and performs postacquisition analysis. Although ATAQS integrates information from online repositories such as protein-protein interactions or cellular functions, this software does not take biological constraints into acc ...
... an analysis pipeline that organizes, generates, and verifies transition lists, and performs postacquisition analysis. Although ATAQS integrates information from online repositories such as protein-protein interactions or cellular functions, this software does not take biological constraints into acc ...
Comparison of random mutagenesis and semi-rational BM3-catalyzed hydroxylation of small alkanes
... 2009; Lassila, 2010) have been described with the aim of generating smaller and/or functionally enriched libraries. These approaches rely on structural and sequence information to design libraries with mutations targeted to specific regions such as the enzyme active site. Such methods may be particu ...
... 2009; Lassila, 2010) have been described with the aim of generating smaller and/or functionally enriched libraries. These approaches rely on structural and sequence information to design libraries with mutations targeted to specific regions such as the enzyme active site. Such methods may be particu ...
substrate specificities of octopine dehydrogenases
... of L-arginine activity), lower rates with alternative guanidino amino acids, L-homoarginine and L-canavanine, and higher apparent K,,'s for alternative keto acids compared to the sea anemone enzyme. Mantle muscle ODH from S. officinalis, with its major physiological role in glycolytic energy product ...
... of L-arginine activity), lower rates with alternative guanidino amino acids, L-homoarginine and L-canavanine, and higher apparent K,,'s for alternative keto acids compared to the sea anemone enzyme. Mantle muscle ODH from S. officinalis, with its major physiological role in glycolytic energy product ...
Purified dextransucrase from Pediococcus pentosaceus CRAG3 as
... milk as per the method described by Bejar et al33. To the semi-skimmed milk (20 mL), sucrose was added at final concentration of 2 and 5% (w/v). The partially purified dextransucrase (20 U/mg, 0.3 mg/mL, 4 mL) from P. pentosaceus CRAG3 (to make final concentration of 5 U/mL) was added to each of the ...
... milk as per the method described by Bejar et al33. To the semi-skimmed milk (20 mL), sucrose was added at final concentration of 2 and 5% (w/v). The partially purified dextransucrase (20 U/mg, 0.3 mg/mL, 4 mL) from P. pentosaceus CRAG3 (to make final concentration of 5 U/mL) was added to each of the ...
Raw Glycerol as Substrate for the Production of Yeast Biomass
... oil and Jatropha curcas oil are the raw materials most frequently used for the production of biodiesel, and glycerol is an inherent byproduct of this reaction [1]. Conversion of the crude product into pure glycerol requires several purification steps, all being energy-consuming and costly processes. ...
... oil and Jatropha curcas oil are the raw materials most frequently used for the production of biodiesel, and glycerol is an inherent byproduct of this reaction [1]. Conversion of the crude product into pure glycerol requires several purification steps, all being energy-consuming and costly processes. ...
PDF Datastream - Brown Digital Repository
... RsaA- using Q-TOF or LTQ-Orbitrap……………………...............................................81 Figure 3.8: Functional classification of total 91 putative surface-exposed proteins identified using both Q-TOF and LTQ-Orbitrap method..................................................82 Figure 3.9: Distribu ...
... RsaA- using Q-TOF or LTQ-Orbitrap……………………...............................................81 Figure 3.8: Functional classification of total 91 putative surface-exposed proteins identified using both Q-TOF and LTQ-Orbitrap method..................................................82 Figure 3.9: Distribu ...
Protein phosphatases and the regulation of mitosis
... et al., 1989). Remarkably, brief treatment of cells with the algal toxin okadaic acid, which is specific for PPP enzymes, triggers many of the changes that are associated with mitosis, including chromatin condensation and structural rearrangement of organelles such as the Golgi apparatus (Lucocq et ...
... et al., 1989). Remarkably, brief treatment of cells with the algal toxin okadaic acid, which is specific for PPP enzymes, triggers many of the changes that are associated with mitosis, including chromatin condensation and structural rearrangement of organelles such as the Golgi apparatus (Lucocq et ...
preprint - Human Genome Center
... In the post-genomic molecular biology, analysis of protein 3-D structures becomes more and more important. Recently, enormous amount of protein 3-D structures are solved and the number of them are still increasing, as seen in the PDB database [Berman et al. 2000]. As a result, faster algorithms to d ...
... In the post-genomic molecular biology, analysis of protein 3-D structures becomes more and more important. Recently, enormous amount of protein 3-D structures are solved and the number of them are still increasing, as seen in the PDB database [Berman et al. 2000]. As a result, faster algorithms to d ...
Protein (nutrient)
Proteins are essential nutrients for the human body. They are one of the building blocks of body tissue, and can also serve as a fuel source. As a fuel, proteins contain 4 kcal per gram, just like carbohydrates and unlike lipids, which contain 9 kcal per gram. The most important aspect and defining characteristic of protein from a nutritional standpoint is its amino acid composition.Proteins are polymer chains made of amino acids linked together by peptide bonds. During human digestion, proteins are broken down in the stomach to smaller polypeptide chains via hydrochloric acid and protease actions. This is crucial for the synthesis of the essential amino acids that cannot be biosynthesized by the body.There are nine essential amino acids which humans must obtain from their diet in order to prevent protein-energy malnutrition. They are phenylalanine, valine, threonine, tryptophan, methionine, leucine, isoleucine, lysine, and histidine. There are five dispensable amino acids which humans are able to synthesize in the body. These five are alanine, aspartic acid, asparagine, glutamic acid and serine. There are six conditionally essential amino acids whose synthesis can be limited under special pathophysiological conditions, such as prematurity in the infant or individuals in severe catabolic distress. These six are arginine, cysteine, glycine, glutamine, proline and tyrosine.Humans need the essential amino acids in certain ratios. Some protein sources contain amino acids in a more or less 'complete' sense. This has given rise to various ranking systems for protein sources, as described in the article.Animal sources of protein include meats, dairy products, fish and eggs. Vegan sources of protein include whole grains, pulses, legumes, soy, and nuts. Vegetarians and vegans can get enough essential amino acids by eating a variety of plant proteins. It is commonly believed that athletes should consume a higher-than-normal protein intake to maintain optimal physical performance.