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Lens Major Intrinsic Protein (MIP)
... turbidity assay. The absorbance time course at 350 nm was monitored when PC-Mbs vesicles (-) and PC vesicles (A)were mixed with 25 pM PS vesicles. Vesicles were incubated in the same solution described for Figure 1. After 3 min of preincubation, 25 r M PS vesicles was added to the cuvette (arrow). e ...
... turbidity assay. The absorbance time course at 350 nm was monitored when PC-Mbs vesicles (-) and PC vesicles (A)were mixed with 25 pM PS vesicles. Vesicles were incubated in the same solution described for Figure 1. After 3 min of preincubation, 25 r M PS vesicles was added to the cuvette (arrow). e ...
Disruption of somitogenesis by a novel dominant allele of Lfng
... our results in tissue culture, we predict that the resulting allele, LfngRLFNG, encodes a protein with the enzymatic activity of LFNG but which is not secreted. This allele does not alter the promoter or enhancer regions, transcription start site, UTRs, or splice sites of Lfng, and thus the mutation ...
... our results in tissue culture, we predict that the resulting allele, LfngRLFNG, encodes a protein with the enzymatic activity of LFNG but which is not secreted. This allele does not alter the promoter or enhancer regions, transcription start site, UTRs, or splice sites of Lfng, and thus the mutation ...
amino acids. combinatorial libraries made up of
... lead to a constant need for new antistaphylococcal agents. In recent years, peptides, either isolated from natural sources or synthetically designed, have emerged as potent antimicrobial agents (for a review, see reference 2). However, the process involved in the development of such new antimicrobia ...
... lead to a constant need for new antistaphylococcal agents. In recent years, peptides, either isolated from natural sources or synthetically designed, have emerged as potent antimicrobial agents (for a review, see reference 2). However, the process involved in the development of such new antimicrobia ...
Ph.D. Thesis Azar Shahpiri
... acid (GA) signal by synthesizing hydrolytic enzymes that are released to the endosperm, before undergoing cell death. The barley (Hordeum vulgare) aleurone layer can be separated from the other seed tissues and maintained in culture, allowing the study of GA, abscisic acid (ABA) and other signals in ...
... acid (GA) signal by synthesizing hydrolytic enzymes that are released to the endosperm, before undergoing cell death. The barley (Hordeum vulgare) aleurone layer can be separated from the other seed tissues and maintained in culture, allowing the study of GA, abscisic acid (ABA) and other signals in ...
REVIEWS - Haseloff Lab
... Based on their position in the UPS, E3s are clearly the key factors that define substrate specificity. To date, four main types of E3s are known in plants, and are classified by their mechanisms of action and subunit composition: HECT, RING, U-box and cullin–RING ligases (CRLs), with the CRLs furthe ...
... Based on their position in the UPS, E3s are clearly the key factors that define substrate specificity. To date, four main types of E3s are known in plants, and are classified by their mechanisms of action and subunit composition: HECT, RING, U-box and cullin–RING ligases (CRLs), with the CRLs furthe ...
A storage form of ribosomes in mouse oocytes
... intact whole oocytes which were then used for making whole mount preparations or for of the beads averaged 212 A while the con embedding and sectioning. DNase had no nections between beads were approx. 1 25 A effect on the lattices, but they were readily in diameter. destroyed by either trypsin or ...
... intact whole oocytes which were then used for making whole mount preparations or for of the beads averaged 212 A while the con embedding and sectioning. DNase had no nections between beads were approx. 1 25 A effect on the lattices, but they were readily in diameter. destroyed by either trypsin or ...
Identification of a Cytoplasm to Vacuole Targeting Determinant in
... a-helix followed by a [3-turn and another a-helix, forming a helix-turn-helix structure. With the use of mutational analysis, we determined that the API propeptide is essential for proper transport into the vacuole. Dele- ...
... a-helix followed by a [3-turn and another a-helix, forming a helix-turn-helix structure. With the use of mutational analysis, we determined that the API propeptide is essential for proper transport into the vacuole. Dele- ...
Partial Purifiaction of β-1,3-glucan synthases and
... chemical modification of the paramylon granule increased immune-potentiation with specific antimicrobial and anti-HIV activities. Due to these specific immune-potentiating activities, paramylon is novel in terms of both structure as well as functional activity. In terms of biotechnological applicati ...
... chemical modification of the paramylon granule increased immune-potentiation with specific antimicrobial and anti-HIV activities. Due to these specific immune-potentiating activities, paramylon is novel in terms of both structure as well as functional activity. In terms of biotechnological applicati ...
InterPro Presentation - European Bioinformatics Institute
... what they intended -To reflect biological reality as accurately as possible in the entry we create by using types, relationships, GO mapping -To provide as much information to the end user as possible about the signature by annotating signatuires and providing links to other databases. ...
... what they intended -To reflect biological reality as accurately as possible in the entry we create by using types, relationships, GO mapping -To provide as much information to the end user as possible about the signature by annotating signatuires and providing links to other databases. ...
Determination of chemical composition of milk marketed in
... Huge part of the population consumed milk because of its nutritional values. It is used in all different periods of life i-e child hood, adolescence, pregnancy and the elderly. It is a sole natural food for first few months of life and it is chiefly valuable as a source of high quality nutrients lik ...
... Huge part of the population consumed milk because of its nutritional values. It is used in all different periods of life i-e child hood, adolescence, pregnancy and the elderly. It is a sole natural food for first few months of life and it is chiefly valuable as a source of high quality nutrients lik ...
Tetrazine−trans-cyclooctene Mediated Conjugation of Antibodies to
... of the bonds under tension19 as well as at high gliding velocities,20 and reduced velocity of transport.21 The noncovalent biotin−streptavidin interaction is short-ranged and reversible, limiting its practical applications: examples are an almost million fold increase in the dissociation constant (K ...
... of the bonds under tension19 as well as at high gliding velocities,20 and reduced velocity of transport.21 The noncovalent biotin−streptavidin interaction is short-ranged and reversible, limiting its practical applications: examples are an almost million fold increase in the dissociation constant (K ...
Kinetics of MPF and histone H1 kinase activity differ during the G2
... protein synthesis block restores the ability of oocytes to activate the M-phase associated protein kinase with a similar dynamic than in ...
... protein synthesis block restores the ability of oocytes to activate the M-phase associated protein kinase with a similar dynamic than in ...
Antibody purification handbook
... diagnostic applications. The use of recombinant technology opens up the potential to create an infinite number of combinations between immunoglobulins, immunoglobulin fragments, tags and selected proteins, further manipulating these molecules to our advantage. The purpose of this handbook is to pres ...
... diagnostic applications. The use of recombinant technology opens up the potential to create an infinite number of combinations between immunoglobulins, immunoglobulin fragments, tags and selected proteins, further manipulating these molecules to our advantage. The purpose of this handbook is to pres ...
Protein targeting, translocation and Escherichia coli Proteomic analysis of substrate-pathway relationships
... So far, β-barrel membrane proteins have only been found in the outer membrane of Gram-negative bacteria and mitochondria, and in the outer envelope of chloroplasts. β-barrel membrane proteins are composed of an even number of anti-parallel β-strands arranged in a barrel like structure. The amide and ...
... So far, β-barrel membrane proteins have only been found in the outer membrane of Gram-negative bacteria and mitochondria, and in the outer envelope of chloroplasts. β-barrel membrane proteins are composed of an even number of anti-parallel β-strands arranged in a barrel like structure. The amide and ...
Lecture 13 Electrophoresis (Part-I)
... charged molecule moves to their counter charge electrodes but electric field is removed before it reaches the electrode. Movement of charged species in an electric field gives differential mobility to the sample based on the charge and consequently resolve them. Movement of the charged particle is r ...
... charged molecule moves to their counter charge electrodes but electric field is removed before it reaches the electrode. Movement of charged species in an electric field gives differential mobility to the sample based on the charge and consequently resolve them. Movement of the charged particle is r ...
Early events in protein folding
... polypeptide chain searches out its final native conformation from an inconceivably large number of available conformations. A polypeptide chain of 101 amino acid residues would have to sample 3100 = 5 × 1047 conformations, if each bond connecting two consecutive residues has only three possible conf ...
... polypeptide chain searches out its final native conformation from an inconceivably large number of available conformations. A polypeptide chain of 101 amino acid residues would have to sample 3100 = 5 × 1047 conformations, if each bond connecting two consecutive residues has only three possible conf ...
Hibernating Bears Conserve Muscle Strength and Maintain Fatigue
... and foot force plate. After 110 d of anorexia and confinement, skeletal muscle strength loss in hibernating bears was about one-half that in humans confined to bed rest. Bears lost 29% of muscle strength over 110 d of denning without food, while humans on a balanced diet but confined to bed for 90 d ...
... and foot force plate. After 110 d of anorexia and confinement, skeletal muscle strength loss in hibernating bears was about one-half that in humans confined to bed rest. Bears lost 29% of muscle strength over 110 d of denning without food, while humans on a balanced diet but confined to bed for 90 d ...
Ammonium toxicity and potassium limitation in yeast.
... controlling the activities and concentrations of enzymes and transporters. One important means of controlling enzyme concentrations is transcriptional regulation [1–3]. In the wake of the genomic sequence, comprehensive measures of transcript levels can be readily obtained. By combining these data w ...
... controlling the activities and concentrations of enzymes and transporters. One important means of controlling enzyme concentrations is transcriptional regulation [1–3]. In the wake of the genomic sequence, comprehensive measures of transcript levels can be readily obtained. By combining these data w ...
Ammonium toxicity and potassium limitation in yeast.
... controlling the activities and concentrations of enzymes and transporters. One important means of controlling enzyme concentrations is transcriptional regulation [1–3]. In the wake of the genomic sequence, comprehensive measures of transcript levels can be readily obtained. By combining these data w ...
... controlling the activities and concentrations of enzymes and transporters. One important means of controlling enzyme concentrations is transcriptional regulation [1–3]. In the wake of the genomic sequence, comprehensive measures of transcript levels can be readily obtained. By combining these data w ...
... Peng, 2012). The lectins that bind to accessible carbohydrate residues from the cell wall or cell membrane trigger a cascade of biological responses. The capacity for identifying and binding glycoconjugates from the microorganism’s surface is exclusive to lectins. Consequently, they are capable of i ...
Protein (nutrient)
Proteins are essential nutrients for the human body. They are one of the building blocks of body tissue, and can also serve as a fuel source. As a fuel, proteins contain 4 kcal per gram, just like carbohydrates and unlike lipids, which contain 9 kcal per gram. The most important aspect and defining characteristic of protein from a nutritional standpoint is its amino acid composition.Proteins are polymer chains made of amino acids linked together by peptide bonds. During human digestion, proteins are broken down in the stomach to smaller polypeptide chains via hydrochloric acid and protease actions. This is crucial for the synthesis of the essential amino acids that cannot be biosynthesized by the body.There are nine essential amino acids which humans must obtain from their diet in order to prevent protein-energy malnutrition. They are phenylalanine, valine, threonine, tryptophan, methionine, leucine, isoleucine, lysine, and histidine. There are five dispensable amino acids which humans are able to synthesize in the body. These five are alanine, aspartic acid, asparagine, glutamic acid and serine. There are six conditionally essential amino acids whose synthesis can be limited under special pathophysiological conditions, such as prematurity in the infant or individuals in severe catabolic distress. These six are arginine, cysteine, glycine, glutamine, proline and tyrosine.Humans need the essential amino acids in certain ratios. Some protein sources contain amino acids in a more or less 'complete' sense. This has given rise to various ranking systems for protein sources, as described in the article.Animal sources of protein include meats, dairy products, fish and eggs. Vegan sources of protein include whole grains, pulses, legumes, soy, and nuts. Vegetarians and vegans can get enough essential amino acids by eating a variety of plant proteins. It is commonly believed that athletes should consume a higher-than-normal protein intake to maintain optimal physical performance.