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Cells: History, Structure, and Function Robert Hooke First to describe cells Used a compound microscope _______________ Actually looking at ___________ cells Antony van Leeuwenhoek Made many microscopes (single lens) Describes several types of cells First to study living, moving organisms with microscope Cell Theory All living things are composed of ______ Cells are the basic units of structure and function of living things New cells are produced from ___________________ Compound Microscope Staph Bacteria Under Compound Microscope Electron Microscopes Transmission (TEM) Specimens cut into very thin slices Beam of electron pass through Scanning (SEM) Samples are dehydrated, put in a vacuum, and sometimes coated in materials like gold Electrons are bounced off the surface Producing 3-D image of surfaces Electron Microscope Staph Bacteria under Electron Microscope Classifying Cells Prokaryote Lack organized structures Circular loop of nucleic acid No ______________ Ex: bacteria and blue-green algae Eukaryote Organized structures called _____________ DNA in ____________ Ex: animal, plant, fungi, and protists Eukaryotic Cells Three Regions _________________ __________ ___________ Cell Membrane Cell Membrane Function Regulate what comes in and out of cell Communication Protection and Support ________________ Double layer of lipid molecules Fluid Mosaic Lipid molecules with other pieces (protein and carbohydrates) embedded Membrane in constant movement Phospholipid •_________ Head •Hydrophilic •Attracted to water •__________ Tail •Hydrophobic •Does not want to be near water Membrane Proteins Allow larger molecules to pass through membrane Can regulate what comes in or out Other Molecules Cholesterol Stabilize the membrane Keep non-polar tails from sticking to each other Carbohydrate Chains _____________________ Cell Wall _____________ Rigid outer layer covering cell membrane Allow plants to support heavy structures like flowers Contains ________ and various proteins Movements Across Membranes __________ Transport Diffusion (Simple and Facilitated) ______________ ___________ Transport Protein Pump Endocytosis and Exocytosis Movement is controlled by ___________ Concentration Amount of _________ (dissolved substance) in a volume of solution Expressed as ________________ Amount of mass is proportional to the concentration Volume is inversely proportional to the concentration Passive Transport Movement from area of ________ concentration to an area of ______ concentration (down concentration gradient) Requires ______ energy Ex: Diffusion (Simple and Facilitated) and Osmosis Diffusion •Movement of _____ from high to low concentrations •Requires no energy •Continues unit equilibrium is reached Facilitated Diffusion Solute cannot simply cross cell membrane because it is semi-permeable Solute movement is aided by ______________________ Allows diffusion of molecules that are too large to diffuse through membrane using simple diffusion Osmosis Movement of ________ from an area of high to low concentration Requires no energy Solutions surrounding cells can be ______tonic – solution has a high [solute] compared to the inside of the cell _____tonic- solution has the same [solute] as the inside of the cell _____tonic- solution has a low [solute] compared to the inside of the cell Active Transport Movement solute from an area of _____ concentration to an area of _______ concentration (across or up the concentration gradient) Requires energy (using ________) Ex: Protein Pumps, Endocytosis, and Exocytosis Protein Pumps Membrane Protein pumps solute across cell membrane Solute moving from low to high concentration Requires ATP energy Endocytosis and Exocytosis Movement of large materials across cell membrane Endocytosis Movement ________ cell Pocket of membrane pinches off to form vesicle (membrane circle surrounding material) Two types Phagocytosis- ______________________ Pinocytosis- _____________________ Exocytosis Movement ________ of cell Phagocytosis and Pinocytosis Exocytosis Cytoplasm Everything inside to cell membrane except for the _____________ Includes the fluid cytosol Where do organelles come from? Thought to originally be prokaryotes that formed a symbiotic relationship with another cell Evidence Many organelles are surrounded by two membranes Some organelles contain their own ______ Nucleus _____________ of Cell Double membrane w/ many __________ Contains DNA Chromatin Chromosomes Nucleolus Small dark region’ Makes __________ Ribosome Makes __________ Two subunits made of RNA and protein Found free floating in cytoplasm or attached to rough ER Endoplasmic Reticulum (ER) Series of membrane bound canals Two Types ____________ Studded w/ ribosomes Processes and transports proteins _____________ No ribosomes Processes and transports lipids Golgi Apparatus Stack of flattened pancake-like membranes Modifies, packages, and ships out lipids and proteins Lysosome Vesicle filled w/ digestive enzymes Breaks down _____________ Aids in programmed cell death (apoptosis) Vacuole Animals Many small membrane bound sacs Storage compartments Plants Usually one large central sac Storage sac and helps establish turgor pressure to keep plant upright Mitochodria Double membrane Inner membrane highly folded _____________of cell (makes ATP’s) Only inherited from mother Contains _______ Chloroplast _______________ Double membrane Converts _______ energy into chemical energy Contains chlorophyll pigment Captures light energy Contains ________ Cytoskeleton Skeleton for Cell Helps cell maintain shape Provides support and protection Aids in movement Made of microtubules and microfilaments Cilia Short, hair-like microtubule extensions Move in ______ motion Move cell or move materials on the surface of cells Cells usually have many Flagella Long, whip-like microtubule extention Move in ______________ fashion Moves cells Cells usually only have one or a few Centrioles Only in ______ cells Grouped microtubules Aids in ___________ Moves chromosomes with spindle fibers Animal vs Plant Cells Organelle Animal Plant Nucleus Cytoplasm Cell membrane YES YES YES YES YES YES Cell wall Lysosome Ribosome ER Mitochondria NO YES YES YES YES YES YES YES YES YES Animal vs Plant Cells (cont) Organelle Animal Plant Golgi YES YES DNA YES YES Vacuole YES (small, several, only YES (large, single) in a few animal cells) Cytoskeleton YES YES Chloroplast NO YES Centriole YES NO Flagella YES (some) NO Cilia YES (some) NO Unicellualer Organisms Organism made of _______ cell Very simple One cell performs all the functions of life Ex: ______________ Multicellular Organisms Organisms made of many cells More complex Cells _______________ and perform certain functions (cell differentiation) All cells work together to perform all the functions of life Ex: _____________________ Levels of Organization Levels of Organization (cont) __________ Molecule Macromolecule ___________ Tissue- cells working together towards a common goal ________- tissue working together towards a common goal Organ system- organ working together towards a common goal __________