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Transcript
Are genes always being transcribed and translated?
Gene Expression
Gene expression for all genes falls into one of two
categories.
constitutive expression –
1.
1.
2.
known as housekeeping genes
induced expression –
lac Operon
lac - lactose
operon –
promoter
gene 1
operator
gene 2
gene 3
lac Operon
Bacterial cells mainly use glucose as a source of
energy.
The lac operon is only turned (on or off?) when
glucose is absent, but lactose is present.
lac Operon
Operon codes for three enzymes
1.
b-galactosidase (lacZ gene)
2. permease (lacY gene)
3. transacetylase (lacA gene)
lac Operon
The lacI gene codes for a separate repressor protein.
lac Operon
repressor protein (lacI gene)
 binds to the
 prevents RNA polymerase from
When do you want the repressor to bind / not bind to
the operon?
When lactose is absent:
When lactose is present:
lac Operon
effector molecule –
inducer –
lac Operon Animation
lac Operon Animation