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Genetics and Genetic Engineering terms agar- porous gelatin like material used as a support matrix for living cells in artificial environments centrifuge device for separating substances on the basis of density clones organisms or cells of nearly identical genetic makeup derived from a single source complementary containing structures that match or bond with related structures electrophoresis technique used to separate migrating molecules in an electrical field Excise to remove a part by a process similar to cutting Expression the appearance of a trait directed by a gene Genetic code a series of three nucleotide bases that determine the amino acid order and the proteins formed in cell reproduction Genetic Engineering technology involved in removing, modifying, or adding genes to a DNA molecule Genetics science dealing with passage of traits from one generation to another Host a cell or organism that harbors another organism or DNA from a foreign source Hybrid offspring or cell originating from parents with differing genetic makeup Ligase enzyme used to join two pieces of DNA Molecular weight sum of the atomic weights of all the atoms in a molecule Mutation a heritable change in genetic makeup Nucleotides building blocks of nucleic acid composed of a sugar, a phosphate and a nitrogenous base Plasmid a small, usually circular, piece of DNA that is separate from the chromosomal DNA Polymerase an enzyme that joins nucleotides to make nucleic acid Recombinant DNA DNA that has been altered by genetic engineering Restriction enzyme restriction endonuclease enzyme that cuts DNA strands at specific sites sequence order of nucleotides or amino acids template a pattern used for developing a complementary structure trait a characteristic resulting from gene expression transform to change the genetic makeup of an organism by alteration of the DNA vector an agent such as a plasmid used to transfer DNA into a host cell Living material Organism - unit of living matter capable of independent function and reproduction Complex Organism higher organism an organism with cells, tissues, and organs arranged to function as a single unit ex. Plants, animals, humans Organ arrangement of tissues which provides life support function for an organism leaf is an organ that carries out respiration, transpiration and ps. Tissue arrangement of cells with similar structures in clusters or sheets for specific function in an organ Cell smallest structural unit of living matter that is capable of carrying out basic life processes Basic Cell structures cell wall - protective layer in plants that keeps the cell intact cell membrane delicate structure that surrounds the cell chloroplasts site of photosynthesis in plant cells contain some DNA Cytoplasm cell fluid containing all cell structures Nucleus control center of a cell contains most of the DNA Chromosomes structures that are physical carriers of genes made p of DNA are located in cell nucleus Mitochondria energy producing structures in the cell cytoplasm contain some DNA Plasmid extra chromosomal piece of DNA located in the cell cytoplasm Ribosome site where new proteins are produced result of genetic expression Cell reproduction mitosis - occurs in body (somatic) cells increases by simple division chromosomes remain paired during cell division meiosis occurs in reproductive cells (gametes) meiosis increases by division into single chromosomes and recombination of male and female cells to form a new individual meiosis one chromosome from each parent forms the new pair each organism has a specific number of paired chromosomes Genetic engineering technology involved in removing, modifying, or adding genes to a DNA molecule Genetic engineering movement of a gene from one organism to another Other names recombinant DNA technology recombinant DNA science gene transfer gene splicing gene cloning Naturally occurring alternations in the genetic makeup of plants and animals allow them to respond to changes in their environment Naturally occurring some bacteria and viruses change the function of cells by inserting their DNA into the DNA of the cell Naturally occurring natural selection ensures that the adapted individual leaves offspring for the next generation Scientific Gen eng. Scientists have developed the ability to transfer genetic material from one organism to another Scientific Gen eng. Plasmids found in some organisms can be engineered to accept DNA from other sources Scientific Gen eng. Restriction enzymes are used to cut the DNA at specific sites cut ends of plasmid rings can accept pieces of DNA from other organisms Scientific Gen eng. The ability to cut and splice DNA segments is providing many opportunities for changing the function of organisms