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NAME: ________________________________________ BIOL 107 A3 - Fall 2007 - Dr. Harrington Midterm Exam October 23, 2007 Version 2 Instructions 1) Make sure that your exam has all 8 pages. 2) Write your name in the space above and your student ID on page 3. 3) Use a soft lead pencil on the General Purpose Answer Sheet. Print your name and your student ID in the correct fields; code them by blackening the corresponding circles. In the field marked special codes enter 2 in column J. 4) This exam has two parts: Part A - 21 multiple choice questions. Answer on the General Purpose Answer Sheet. Part B - 4 written answer questions. Answer in the space provided. 5) No source materials or electronic devices are permitted. 6) You have 75 minutes to complete this exam. BIOL 107 A3 -- Fall 2007 -- Midterm exam 2 Part A Multiple choice questions Choose the one alternative that best completes the statement or answers the question. (1 mark each) 1) A chemical reaction in a cell would be coupled to ATP hydrolysis to: A. make the overall process exergonic B. make the overall process endergonic C. lower the energy of activation D. raise the energy of activation 2) A chemical reaction in a cell would be catalyzed by an enzyme to: A. make the overall process exergonic B. make the overall process endergonic C. lower the energy of activation D. raise the energy of activation 3) When the plant Arabidopsis thaliana is cold it _____ the production of unsaturated fatty acids to _____ the fluidity of its plasma membrane. A. increases ... increase B. increases ... decrease C. decreases ... increase D. decreases ... decrease 4) The _____ model of the Golgi apparatus states that transport vesicles from the ER fuse to form the _____ cisternae. A. cisternal maturation ... trans B. cisternal maturation ... cis C. stationary cisternae ... trans D. stationary cisternae ... cis 5) Pyrococcus furiosus is a marine organism that grows best at 100 oC. This species is most likely to be a/an: A. Protist B. Bacteria C. Archaea D. Fungus E. Animal 6) The amino acid serine (R group --CH2OH) contains _____ oxygens and _____ carbons when it is free in solution. (Hint: draw it) A. 2 ... 3 B. 3 ... 3 C. 3 ... 4 D. 4 ... 3 E. 4 ... 4 BIOL 107 A3 -- Fall 2007 -- Midterm exam Marks for Section B 3 Student ID _________________ Question 22 /6 Question 23 /4 Question 24 /4 Question 25 /4 Subtotal / 18 ****************************************************************************************** 7) The enzyme Adh (Alcohol dehydrogenase) is made from two identical polypeptides. Proteins such as this are said to have _____ structure. A. primary B. secondary C. tertiary D. quaternary 8) Mycoplasmas are small bacteria that lack cell walls. They must live in a/an _____ environment. A. hypertonic B. hypotonic C. isotonic D. hypertonic or hypotonic E. hypotonic or isotonic 9) CFTR is a human plasma membrane glycoprotein. If a cell is currently synthesizing CFTR in what areas of the cell will these proteins be found? A. vesicles, vacuoles, and Golgi apparatus B. Golgi apparatus and roughER C. roughER, smoothER, and Golgi apparatus D. Golgi apparatus, vesicles, and roughER E. vesicles, vacuoles, roughER, Golgi apparatus, and smoothER BIOL 107 A3 -- Fall 2007 -- Midterm exam 4 10) Aspirin (ASA) is often given to a person during a heart attack because Aspirin _____ responsible for blood platelet aggregation. A. inhibits the COX-1 enzyme B. inhibits the COX-2 enzyme C. activates the COX-1 enzyme D. activates the COX-2 enzyme 11) The existence of life does not violate the second law of thermodynamics because growth of an organism: A. increases the organism’s entropy B. does not create or destroy energy C. is dependent upon energy released by the sun D. causes a net increase in entropy in the universe E. consumes heat 12) The first step in bacterial conjugation is the: A. synthesis of the conjugation bridge by the F+ cell B. synthesis of the conjugation bridge by the F- cell C. synthesis of the sex pilus by the F+ cell D. synthesis of the sex pilus by the F- cell 13) Peroxisomes form from vesicles that leave the ____ ; their internal proteins are synthesized in the _____ and then imported into the peroxisomes. A. ER ... cytosol B. Golgi apparatus ... cytosol C. Golgi apparatus ... ER D. Golgi apparatus ... Golgi apparatus E. plasma membrane ... Golgi apparatus 14) Within a muscle cell the concentration of Ca++ is higher in the smooth ER than in the cytosol. ATP powered Ca++ Transporters in the smooth ER membrane are needed for the movement of Ca++ _____. This causes the _____ of the cell. A. from the cytosol to the smooth ER ... relaxation B. from the cytosol to the smooth ER ... contraction C. from the smooth ER to the cytosol ... relaxation D. from the smooth ER to the cytosol ... contraction 15) The contractile vacuoles found in freshwater protists such as Paramecium allow the cells to withstand: A. plasmolysis in this isotonic environment B. plasmolysis in this hypertonic environment C. plasmolysis in this hypotonic environment D. the influx of water in this hypertonic environment E. the influx of water in this hypotonic environment BIOL 107 A3 -- Fall 2007 -- Midterm exam 5 16) How much DNA is found in a typical human cell? A. 6400 basepairs (bp) B. 6400 nucleotides (nt) C. 6400 kilobases (kb) D. 6400 megabases (Mb) 17) A phospholipid in the plasma membrane in one cell may end up in the plasma membrane of a neighboring cell if the cells are connected with: A. tight junctions B. plasmodesmata C. gap junctions D. desmosomes E. any of the above 18) The Sucrase enzyme which breaks down sucrose catalyzes an _____ reaction. A. endergonic, catabolic, condensation B. endergonic, anabolic, condensation C. exergonic, anabolic, hydrolysis D. exergonic, catabolic, condensation E. exergonic, catabolic, hydrolysis 19) Where is DNA found in a plant cell? A. nucleus B. nucleus and chloroplasts C. nucleus and mitochondria D. nucleus, mitochondria, and chloroplasts E. nucleus, mitochondria, peroxisomes, and chloroplasts 20) The E. coli cell uses the _____ through the basal apparatuses to cause _____ of its flagella. A. entry of protons into the cell ... bending B. entry of protons into the cell ... rotation C. exit of protons from the cell ... bending D. exit of protons from the cell ... rotation 21) How many BIOL 107 podcasts have you listened to? (Each choice is correct and is worth a mark.) A. none B. just the midterm podcasts C. a few of the lecture and midterm podcasts D. about half of the podcasts E. most or all of the podcasts BIOL 107 A3 -- Fall 2007 -- Midterm exam 6 Part B Written answer questions Question 22 Draw a well labeled diagram showing how this macrophage (left) would approach, engulf, and digest this bacteria cell (right). Assume that everything needed for these processes has already been synthesized within the cell. (6 marks) BIOL 107 A3 -- Fall 2007 -- Midterm exam 7 Question 23 This figure shows a cross section through a bacterial cell. Answer the following questions. (0.5 marks each) a) Name molecule (a) ___________________________ b) Name molecule (b) ___________________________ c) Name molecule (c) ___________________________ d) Name molecule (d) ___________________________ e) Name the type of cell wall shown ___________________________ f) Indicate on the diagram where the cell wall is. g) Which molecule: a, b, c, or d is poisonous to humans? ________ h) Penicillin halts the synthesis of which molecule: a, b, c, or d? ________ BIOL 107 A3 -- Fall 2007 -- Midterm exam 8 Question 24 Short answer questions. (2 marks each) a) Humans are of what genus and species? (Write your answer in the proper format.) ________________________ b) Describe two of the similarities between collagen and cellulose. Question 25 Singer and Nicolson used the following experiments to support their 1972 Fluid Mosaic Model of the plasma membrane. For each experiment describe what the results were (in a single sentence) and why these results were obtained (in a second sentence). (2 marks each) a) Gorter and Grendel (1925) compared the amount of phospholipids present in red blood cells to the surface area of these cells. b) Frye and Edidin (1970) fused human and mouse cells on which the human membrane proteins were glowing red and the mouse membrane proteins were glowing green.