Download SLG MOCK MIDTERM – FOR PRACTICE ONLY

PASS MOCK EXAM
– FOR PRACTICE ONLY –
Course: BIOL 1103
Facilitator: Jillian Rohonczy
Dates and locations of mock exam take-up: Wednesday, November 11th 7:00 pm – 8:30 pm in AT 101 & Thursday,
November 12th 4:30 pm – 6:00 pm in SA 416.
It is most beneficial to you to write this mock midterm UNDER EXAM CONDITIONS. This means:
• Complete the midterm in _1_ hour(s).
• Work on your own.
• Keep your notes and textbook closed.
• Attempt every question.
After the time limit, go back over your work with a different colour or on a separate piece of paper and
try to do the questions you are unsure of. Record your ideas in the margins to remind yourself of what
you were thinking when you take it up at PASS.
The purpose of this mock exam is to give you practice answering questions in a timed setting and to
help you to gauge which aspects of the course content you know well and which are in need of further
development and review. Use this mock exam as a learning tool in preparing for the actual exam.
Please note:

Come to the PASS session with your mock exam complete. There, you can work with other
students to review your work.

Often, there is not enough time to review the entire exam in the PASS session. Decide which
questions you most want to review – the Facilitator may ask students to vote on which
questions they want to discuss.

Facilitators do not bring copies of the mock exam to the session. Please print out and complete
the exam before you attend.
 Facilitators do not produce or distribute an answer key for mock exams. Facilitators help
students to work together to compare and assess the answers they have. If you are not able to
attend the PASS session, you can work alone or with others in the class.
Good Luck writing the Mock Exam!!
DISCLAIMER: PASS handouts are designed as a study aid only for use in PASS workshops. Handouts may
contain errors, intentional or otherwise. It is up to the student to verify the information contained within.
1. Which of the following statements about Light Dependent Reactions is FALSE?
a. Pheophytin is the primary electron acceptor in Photosystem II.
b. Photosystem I has a reaction centre with an absorption peak of 680 nm.
c.
P680’s lost electron is replenished through the splitting of a water molecule.
d. The energy lost through the electron transport chain is used to pump protons across the thylakoid
membrane to create a pmf to drive ATP synthase.
e. The electrons from Photosystem I are donated to ferredoxin and used to reduce NAPD+ to NADPH.
2. Which of the following statements about the DNA double helix is true?
a. DNA strands have directionality: a 5’ and a 3’ end
b. The two strands are antiparallel
c.
The two strands are held together by hydrogen bonds between complementary bases
d. Nucleotides are joined together by phosphodiester bonds
e. All of the above
3. Place the following terms in order of lowest to highest level of organization.
i.
Nucleosome ii. DNA iii. Metaphase Chromosome iv. 300 nm Looped Domains v. 30 nm chromatin fibre
a. ii, iv, v, i, iii
b. ii, i, v, iv, iii
c. iii, v, iv, ii, i
d. iii, iv, v, i, ii
e. v, i, iv, iii, ii
4. What is the net expenditure for each 3-carbons of sugar made during the Calvin Cycle (Photosynthesis)?
a. 3 ATP and 3 NADPH
b. 6 ATP and 9 NADPH
c.
6 ATP and 6 NADPH
d. 9 ATP and 6 NADPH
e. 9 ATP and 9 NADPH
Use the following image to answer questions 6 and 7.
5. Which molecule is depicted above?
a. RNA Guanine
b. DNA Adenine
c. DNA Guanine
d. DNA Thymine
e. RNA uracil
6. According to complementary base pairing, the above molecule would bind with…
a. DNA Adenine
b. RNA Uracil
c.
DNA Cytosine
d. RNA Adenine
e. DNA Guanine
7. Which of the following relieves over twisting ahead of the replication fork during DNA replication?
a. Helicase
b. Topoisomerase
c.
DNA Ligase
d. Single-stranded binding proteins
e. DNA Polymerase III
8. What did the experiment conducted by Meselson and Stahl prove?
a. The DNA replication is conservative
b. The DNA replication follows and dispersive model
c.
That telomeres protect the ends of chromosomes
d. That the shortening of telomeres is connected to aging
e. That DNA replication is semiconservative
9. If an mRNA sequence is 5’- UGAACCUGA- 3’, what is the sequence of the DNA template strand?
a. 5’ - TCAAGGTCA - 3’
b. 5’- TGAACCTGA – 3’
c.
3’ - ACTTGGACT- 5’
d. 5’ – ACTTGGACT- 3’
e. 3’ – TGAAGGTGA - 5’
10. Which of the following statements about alternate methods of carbon fixation in plants is true?
a. In C4 and CAM plants the CO2 is first fixed into an organic acid, malate.
b. C4 plants only admit CO2 at night.
c.
CAM plants admit CO2 at night.
d. Both a and b are correct
e. Both a and c are correct
11. Where are photosystems II and I located?
a. Stroma of the chloroplast
b. Thylakoid membrane
c.
Thylakoid space
d. Outer membrane of the chloroplast
e. Cytoplasm surrounding the chloroplast
12. Which of the following statements about the Calvin Cycle is true?
a. It is carried out in the Thylakoid membrane.
b. It uses ATP and NADPH to convert CO2 to the sugar G3P.
c.
Converts solar energy to chemical energy in the form of ATP and NADPH.
d. During the Calvin Cycle the splitting of H2O occurs leading to the release of O2 to the atmosphere.
e. All of the above
13. Which of the following histones interacts with the nucleosome to facilitate higher orders of packing?
a. H3
b. H2B
c.
H4
d. H1
e. H2A
14. A promoter…
a. Contains an initiation site
b. Is the region of the DNA that includes the site where RNA polymerase binds
c.
Contains a recognition sequence (TATA box in eukaryotes and Pribnow box in prokaryotes)
d. All of the above
e. None of the above
15. Which of the following serves as an adapter molecule during protein synthesis?
a. tRNA
b. rRNA
c.
mRNA
d. tRNA & mRNA
e. rRNA & tRNA
16. If there is a change in one DNA base pair that results in the substitution of one amino acid for another in the
protein made by a gene, this is a ________?
a. Missense mutation
b. Nonsense mutation
c.
Frameshift mutation
d. Silent mutation
e. None of the above
17. Which of the following signals the termination of transcription?
a. TATA Box
b. Pribnow Box
c.
GTP
d. Aminoacyl rRNA synthetase
e. Polyadenylation
18. During which phase of the cell cycle do eukaryotes undergo replication?
a. G0
b. G1
c.
S phase
d. G2
e. M phase
19. Thymine makes up 36% of the nucleotides in a sample of DNA from an organism. Approximately what
percentage of nucleotides in this sample will be Guanine?
a. 36%
b. 28%
c.
14%
d. 7%
e. It cannot be determined from the information provided.
20. Which of the following statements describes the concept of “semi-conservative” DNA replication?
a. The two parental strands reassociate after acting as templates for new strands, thus restoring the
parental double helix.
b. Each strand of both daughter molecules contains a mixture of old and newly synthesized DNA.
c.
The two strands of the parental molecule separate, and each functions as a template for synthesis of a
new, complementary strand.
d.
DNA Polymerase III carries out synthesis by extending from the RNA primer (5’ to 3’)
e.
That the lagging strand of DNA is synthesized in short fragments called Okazaki fragments.
21. Which of the following statements about DNA replication in prokaryotes is true?
a. Leading strand synthesis is discontinuous
b. DNA polymerase III carries out DNA synthesis from 3’ to 5’
c.
DNA Ligase separates the two strands of DNA
d. DNA polymerase I removes the RNA primer and replaces it with DNA nucleotides
e. Lagging strand synthesis is continuous
22. Which of the following stabilize single-stranded DNA and prevents the two strands from reforming doublestranded DNA?
a. Helicase
b. Topoisomerase
c.
DNA Ligase
d. Single-stranded binding proteins
e. DNA Polymerase III
23. Which of the following statements is false?
a. The centrosome is the microtubule organizing centre
b. Kinetochores at the centromere of the chromatid are where microtubules attach during prometaphase
c.
Kinetochores of sister chromatids are attached to microfilaments from opposite poles.
d. There are a pair of centrioles within the centrosome of an animal cell
e. Both a and c are false
24. What are exons?
a. Sequences of a gene that are transcribed into RNA but are subsequently excised during processing.
b. Sequences of a gene that are transcribed into RNA and will encode a protein.
c.
Enzymes that move along the DNA duplex and separate the strands and copies RNA from the DNA
template by adding nucleotides to the 3’ end of the growing RNA strand.
d. Enzymes that can transcribe 30-60 nucleotides per second.
e. Sequences that signal transcription termination.
25. Given the following sequence, match the correct amino acid sequence. (Genetic code table on last page)
5’ – ….UUAGAUGGCAGCUGU… – 3’
a. Leucine, Aspartic Acid, Glycine, Serine, Cysteine
b. Leucine, Glutamic Acid, Alanine, Serine, Cysteine
c.
Phenylalanine, Aspartic Acid, Proline, Tyrosine, Valine
d. Isoleucine, Arginine, Tyrosine. Glycine, Leucine
26. Which of the following enzymes is responsible for amino acid activation?
a. Aminoacyl rRNA synthetase
b. Animoacyl tRNA synthetase
c.
Aminoacyl mRNA synthetase
d. ATP synthetase
e. None of the above
27. If there is a deletion of a base in a coding region of a gene this could results in a_________.
a. Missense mutation
b. Nonsense mutation
c.
Frameshift mutation
d. Silent mutation
e. None of the above
28. Which of the following sites located in the ribosomal large subunit holds the tRNA carrying the growing
polypeptide chain?
a. A site
b. P site
c.
E site
d. D site
e. F site
29. Which of the following is incorrectly paired?
a. Prophase – Mitotic spindle begins to form
b. Prometaphase – Nuclear envelope fragments
c.
Metaphase – Chromosomes are pulled in opposite directions by kinetochore microtubules
d. Anaphase – Nuclear envelope starts to reform
e. Telophase – Cytoplasm begins to divide in two
30. Given the following mRNA sequence, what are the last two amino acids before the stop codon? (Genetic
code table on last page)
5’ – …UCGAAACGCGGGUAGUA…– 3’
a. Proline, Valine
b. Tyrosine, Glycine
c.
Arginine, Glycine
d. Arginine, Valine
e. Leucine, Histidine
31. What is the main function of the Poly(A) tail?
a. Promotes export of mRNA from the nucleus
b. Protection of mRNA from degradation
c.
Removes introns from pre-RNA
d. Is the protein synthesis termination site
e. Both a and b are correct
32. Which of the following statements about replication in prokaryotes is false?
a. Division occurs through binary fission
b. Most prokaryotes have a single, circular DNA molecule.
c.
Replication of bacterial chromosome begins at the origin of replication
d. Active movement distributes two replicated chromosomes to two ends of the cell
e. None of the above
33. Where does transcription occur and where does translation occur in eukaryotic cells?
a. Cytoplasm; Nucleus
b. Cytoplasm; Cytoplasm
c.
Nucleus; Nucleus
d. Nucleus; Cytoplasm
e. Lysosome; Ribosome
34. Which of the following best describes mismatch repair?
a. Specialized enzymes check the newly synthesized DNA duplex and remove any incorrectly matched base
pairs.
b. When polymerase checks the result and removes incorrect base pairs.
c.
The ability of polymerase to insert the correct nucleotide.
d. When sequence changes in the DNA occur and become permanent.
e. When DNA repair enzymes attempt to repair damage to the DNA prior to replication.