Download File - Biology with Radjewski

AP Biology EXAM: MMGDTTGE
Mitosis and Meiosis (5 m/c)
 Haploid vs. diploid
 Products of mitosis vs. products of meiosis
 Cell Cycle
 Know your diagram of the phases for mitosis and meiosis – be able to identify IPMATC
Genetics (5 m/c + short answer)
 Given a scenario, be able to determine what trait is dominant etc.
o Dominant vs. recessive
o Homozygous vs. heterozygous
o Codominant
 Be able to do a bloodtyping problem
o A
o B
o AB
o O
 Pedigree will be given. Can you determine the inheritance?
o Autosomal dominant vs. Autosomal recessive
o Sex Linked dominant vs. Sex Linked recessive
 Chi Square sex linked genetic problem. See example attached.
DNA Structure and Replication (5 m/c)
 History of DNA
o Griffith
o Avery and MacLeod
o Watson and Crick
o Rosalind Franklin
o Chargaff
 Know structure of DNA (diagram of DNA will be given)
o Hydrogen bond vs. phosphodiester bond
o Nucleotide
o Pyrimidine vs. purines
o Sugar vs. phosphate
o 5’ and 3’
o ACTG
 Given DNA template sequence, be able to give the complement. Example: DNA
template is 5’ACTGAC3’. The complement is_____________________
 DNA replication
o DNA polymerase vs. DNA helicase
o Direction
o DNA ligase
o Location in cell
o When in cell cycle does it occur?
Protein Synthesis (5 m/c)
 If given the DNA template or complement strand, can you give the mRNA sequence?
Example: DNA template is 5’ACTTGC3’, the mRNA reads _________________
 Introns vs. exons
o premRNA vs. mature mRNA
o spliceosome
o snRNP
 5’ GTP cap
 Poly A tail
 Genetic code
 Mutations
o Silent
o Missense
o Frameshift
Operons (5 m/c)
 Negative regulation
 Positive regulation
 Lac operon
o Promoter
o Operator
o Structural genes
o repressor
o On vs. off
o What types of cells have this?
o Role of lactose (or allolactose)
 Lac operon vs. trp operon
Genetic Engineering (5 m/c + plasmid mapping)
 Restriction Enyzmes
 Sticky ends
 Hydrogen bonds
 DNA charge
 Direction DNA migrates in gel electrophoresis
 Which sized DNA fragments move farthest?
 Be able to analyze a gel (like on lab quiz)
 How is DNA technology used to insert a gene of interest into a bacterium?
 How can you identify recombinant bacteria?
 How can you verify that the gene of interest in expressed?
 How can a GMO provide a benefit to humans AND also be a threat to a
population/ecosystem?
 Be able to construct a plasmid map from sample gel with 2 restriction enzymes