Download Biocompatibility of Sapphire and Borosilicon Glass

Biocompatibility of Sapphire and Borosilicon
Glass Using Combined MRI and Histology
Kaushik S Parthasarathy
Aim of the Research
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We developed a Protocol to investigate the short and long term neuro compatibility of
novel and traditional Biomaterials that could be used in Neural Prosthesis
Wafers of Sapphire and BSG (2.5mm dia. x 0.250mm thick) were implanted
unilaterally onto the surface of the parietal cortex using aseptic techniques in rats and
imaged using MRI (4.7T Animal Magnet)
Experiments….
• The animals were allowed to survive for 28 days
• Sham – operated and mechanically – lesioned animals served as
controls
• MRI
 Pre and Post implantation (10, 28 days) MRI was performed
using Spin Echo and Gradient Echo Sequence, on sham, +ve
control and implanted animal
 Assessment of Inflammation, Edema and Tissue interference
with the implant was done using MRI
• Histology
 Tissue underlying the implant was stained for degenerating
axons and neurons using Nissl, Silver, GFAP for reactive
astrocytes stains
Results
Sham
10th Day
10th Day
28th Day
28th Day
Histology
Nissl Stain
Sapphire Implant
10th Day
28th Day
Histology
Nissl Stain
GFAP
Silver
Future Directions
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Surface modification is done in order to enhance the biocompatibility of the
materials
“GOAL”
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The overall goal of the project is to provide comprehensive evaluations of
materials and neural implants in the most appropriate (in the retina and the
brain where the structures will be implanted for treatment or diagnoses)
setting.
Acknowledgements
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Michigan Life Science Corridor (MLSC) for funding the Research
E.Mark Haacke
Dr.Pat McAllister
Dr.G.Auner
Dr.Norman Cheng
Yimin Shen
Jie Li
Kelley Barbant