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Each organism has a unique combination of traits encoded in molecules of ... • • • • a. DNA b. protein c. enzymes d. RNA Which of the following types of RNA carries instructions for making protein. • • • • a. b. c. d. rRNA mRNA tRNA RNAi During transcription… • • • • a. proteins are synthesized b. DNA is replicated c. RNA is produced d. Ribosomes “read” RNA molecules A repressor protein… • a. blocks movement of RNA polymerase • b. prevents DNA synthesis • c. attaches to ribosomes during translation • d. is encoded by one of the structural genes. The lac operon is “shut off” when… • a. glucose is absent • b. glucose is present • c. lactose is absent • d. lactose is present • e. None of the above. Enzymes that cut DNA molecules at specific sites… • a. are restriction endonucleases. • b. work best in mammals • c. are not needed in DNA fingerprinting • d. all of the above. Antibiotics such as ampicillin… • a. are formed by plasmids • b. may prevent bacteria from reproducing • c. allow bacteria to glow in the dark as long as the plasmid is present. • d. none of the above If linear DNA is restricted in 3 sites… • a. 4 bands will appear on the gel • b. 3 bands will appear on the gel • c. 5 bands will appear on the gel • d. 1 band will appear on the gel • e. not enough information is provided by the question. E. coli is a great organism for transformation because… • a. it reproduces slowly • b. it is difficult to destroy in the lab • c. it is single-celled • d. all of the above • e. 2 of the above (a-c) Arabinose… • a. is a protein that can cause the repressor protein to release from the operator. • b. binds to RNA polymerase and changes its shape. • c. is a protein that is needed to make GFP. • d. is produced by the structural genes • e. is a sugar that is needed to make GFP. Most E. coli will grow on LB agar. • a. False • b. True Genes… • a. encode for nucleic acids • b. are short sections of RNA that encode for proteins • c. cannot be mutated. • d. are short sections of DNA that encode for nucleic acids. • e. encode for proteins. RNA polymerase… • a. translates mRNA into nucleic acids • b. transcribes mRNA into nucleic acids • c. copies DNA into tRNA • d. copies mRNA into proteins • e. none of these All of the following are parts of an RNA nucleotide except for... • • • • • a. ribose b. phosphates c. .uracil d. deoxyribose e. all of these are present Calcium Chloride… • a. opened the pores in the bacterial cell wall. • b. changed the charge on the plasmid to positive. • c. changed the charge on the cell wall to positive. • d. allowed the pGLO to accept arabinose. • e. gave the bacteria resistance to ampicilin. A 14bp DNA fragment will… • a. travel the same distance as a 20bp DNA fragment • b. travel a shorter distance than a 20bp DNA fragment • c. is a protein that is needed to make GFP. • d. be a darker band on the gel than a 20bp DNA fragment. • e. travel further than a 20bp DNA fragment Colonies appeared on this plate during the transformation lab… • a. LB agar alone • b. LB agar with ampicilin and the (-) tube of bacteria • c. LB agar with ampicilin and the (+) tube of bacteria • d. all of the plates had colonies A plasmid of 600bp is cut by a restriction enzyme in 3 sites has fragments of 120bp, 220bp, and… • a. 600bp • b. 120bp • c. 220bp • d. 360bp • e. 260bp The –OH (hydroxyl) group is found on the ___carbon of deoxyribose. • a. 1’ • b. 3’ • c. 5’ • d. 2’ and 3’ • a. Primary Protein • b. Secondary Protein. • c. Tertiary Protein. • d. Quaternary Protein. Lowest concentration of salt • a. Elution Buffer • b. Wash Buffer • c. Equilibration Buffer • d. Binding Buffer Hydrophobic proteins are… • a. Nonpolar and dissolve in water. • b. Nonpolar and don’t dissolve in water. • c. Polar and dissolve in water. • d. Polar and don’t dissolve in water. The End. How’d you do?