Download Semester Test Practice Test

Each organism has a unique
combination of traits encoded in
molecules of ...
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•
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•
a. DNA
b. protein
c. enzymes
d. RNA
Which of the following types of
RNA carries instructions for
making protein.
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•
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•
a.
b.
c.
d.
rRNA
mRNA
tRNA
RNAi
During transcription…
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•
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a. proteins are synthesized
b. DNA is replicated
c. RNA is produced
d. Ribosomes “read” RNA
molecules
A repressor protein…
• a. blocks movement of RNA
polymerase
• b. prevents DNA synthesis
• c. attaches to ribosomes during
translation
• d. is encoded by one of the structural
genes.
The lac operon is “shut off”
when…
• a. glucose is absent
• b. glucose is present
• c. lactose is absent
• d. lactose is present
• e. None of the above.
Enzymes that cut DNA molecules
at specific sites…
• a. are restriction endonucleases.
• b. work best in mammals
• c. are not needed in DNA fingerprinting
• d. all of the above.
Antibiotics such as ampicillin…
• a. are formed by plasmids
• b. may prevent bacteria from reproducing
• c. allow bacteria to glow in the dark as
long as the plasmid is present.
• d. none of the above
If linear DNA is restricted in 3
sites…
• a. 4 bands will appear on the gel
• b. 3 bands will appear on the gel
• c. 5 bands will appear on the gel
• d. 1 band will appear on the gel
• e. not enough information is provided by
the question.
E. coli is a great organism for
transformation because…
• a. it reproduces slowly
• b. it is difficult to destroy in the lab
• c. it is single-celled
• d. all of the above
• e. 2 of the above (a-c)
Arabinose…
• a. is a protein that can cause the repressor
protein to release from the operator.
• b. binds to RNA polymerase and changes its
shape.
• c. is a protein that is needed to make
GFP.
• d. is produced by the structural genes
• e. is a sugar that is needed to make GFP.
Most E. coli will grow on LB
agar.
• a. False
• b. True
Genes…
• a. encode for nucleic acids
• b. are short sections of RNA that encode
for proteins
• c. cannot be mutated.
• d. are short sections of DNA that encode
for nucleic acids.
• e. encode for proteins.
RNA polymerase…
• a. translates mRNA into nucleic acids
• b. transcribes mRNA into nucleic acids
• c. copies DNA into tRNA
• d. copies mRNA into proteins
• e. none of these
All of the following are parts
of an RNA nucleotide except
for...
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a. ribose
b. phosphates
c. .uracil
d. deoxyribose
e. all of these are present
Calcium Chloride…
• a. opened the pores in the bacterial cell
wall.
• b. changed the charge on the plasmid to
positive.
• c. changed the charge on the cell wall to
positive.
• d. allowed the pGLO to accept
arabinose.
• e. gave the bacteria resistance to
ampicilin.
A 14bp DNA fragment will…
• a. travel the same distance as a 20bp DNA
fragment
• b. travel a shorter distance than a 20bp DNA
fragment
• c. is a protein that is needed to make
GFP.
• d. be a darker band on the gel than a 20bp
DNA fragment.
• e. travel further than a 20bp DNA fragment
Colonies appeared on this plate
during the transformation lab…
• a. LB agar alone
• b. LB agar with ampicilin and the (-)
tube of bacteria
• c. LB agar with ampicilin and the (+)
tube of bacteria
• d. all of the plates had colonies
A plasmid of 600bp is cut by a
restriction enzyme in 3 sites has
fragments of 120bp, 220bp, and…
• a. 600bp
• b. 120bp
• c. 220bp
• d. 360bp
• e. 260bp
The –OH (hydroxyl) group is found
on the ___carbon of deoxyribose.
• a. 1’
• b. 3’
• c. 5’
• d. 2’ and 3’
• a. Primary
Protein
• b. Secondary
Protein.
• c. Tertiary
Protein.
• d. Quaternary
Protein.
Lowest concentration of salt
• a. Elution Buffer
• b. Wash Buffer
• c. Equilibration Buffer
• d. Binding Buffer
Hydrophobic proteins are…
• a. Nonpolar and dissolve in water.
• b. Nonpolar and don’t dissolve in
water.
• c. Polar and dissolve in water.
• d. Polar and don’t dissolve in
water.
The End.
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