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Chapter 15 From Genes to Proteins Question? How does DNA control a cell? By controlling Protein Synthesis. Proteins are the link between genotype and phenotype. For tests: Name(s) of experimenters Outline of the experiment Result of the experiment and its importance 1909 - Archibald Garrod Suggested genes control enzymes that catalyze chemical processes in cells. Inherited Diseases - “inborn errors of metabolism” where a person can’t make an enzyme. Example Alkaptonuria - where urine turns black after exposure to air. Lacks - an enzyme to metabolize alkapton. George Beadle and Edward Tatum Worked with Neurospora and proved the link between genes and enzymes. Neurospora Pink bread mold Experiment Grew Neurospora on agar. Varied the nutrients. Looked for mutants that failed to grow on minimum agar. Results Three classes of mutants for Arginine Synthesis. Each mutant had a different block in the Arginine Synthesis pathway. Conclusion Mutations were abnormal genes. Each gene dictated the synthesis of one enzyme. One Gene - One Enzyme Hypothesis. Current Hypothesis One Gene - One Polypeptide Hypothesis (because of 4th degree structure). Central Dogma DNA Transcription RNA Translation Polypeptide Explanation DNA - the Genetic code or genotype. RNA - the message or instructions. Polypeptide - the product for the phenotype. Genetic Code Sequence of DNA bases that describe which Amino Acid to place in what order in a polypeptide. The genetic code gives the primary protein structure. Code Basis If you use: 1 base = 1 amino acid 4 bases = 4 amino acids 41 = 4 combinations, which are not enough for 20 AAs. If you use: 2 bases = 1 amino acid 42 = 16 amino acids Still not enough combinations. If you use: 3 bases = 1AA 43 = 64 combinations More than enough for 20 amino acids. Genetic Code Is based on triplets of bases. Has redundancy; some AA's have more than 1 code. Proof - make artificial RNA and see what AAs are used in protein synthesis (early 1960’s). Codon 3-nucleotide “word” in the Genetic Code. 64 possible codons known. A Codon Dictionary Start- AUG (Met) Stop- UAA UAG UGA 60 codons for the other 19 AAs. For Testing: Be able to “read” a DNA or RNA message and give the AA sequence. RNA Genetic Code Table will be provided. Code Redundancy Third base in a codon shows "wobble”. First two bases are the most important in reading the code and giving the correct AA. The third base often doesn’t matter. Code Evolution The genetic code is nearly universal. Ex: CCG = proline (all life) Reason - The code must have evolved very early. Life on earth must share a common ancestor. Reading Frame and Frame Shift The “reading” of the code is every three bases (Reading Frame) Ex: the red cat ate the rat Frame shift – improper groupings of the bases Ex: thr edc ata tat her at The “words” only make sense if “read” in this grouping of three. Transcription Process of making RNA from a DNA template. Transcription Steps 1. 2. 3. 4. RNA Polymerase Binding Initiation Elongation Termination RNA Polymerase Enzyme for building RNA from RNA nucleotides. Binding Requires that the enzyme find the “proper” place on the DNA to attach and start transcription. Binding Is a complicated process Uses Promoter Regions on the DNA (upstream from the information for the protein) Requires proteins called Transcription Factors. Transcription Initiation Complex The complete assembly of transcription factors and RNA Polymerase bound to the promoter area of the DNA to be transcribed. Initiation Actual unwinding of DNA to start RNA synthesis. Requires Initiation Factors. Elongation RNA Polymerase untwists DNA 1 turn at a time. Exposes 10 DNA bases for pairing with RNA nucleotides. Elongation Enzyme moves 5’ 3’. Rate is about 60 nucleotides per second. Comment Each gene can be read by sequential RNA Polymerases giving several copies of RNA. Result - several copies of the protein can be made. Termination DNA sequence that tells RNA Polymerase to stop. Ex: AATAAA RNA Polymerase detaches from DNA after closing the helix. Final Product Pre-mRNA This is a “raw” RNA that will need processing. Modifications of RNA 1. 5’ Cap 2. Poly-A Tail 3. Splicing 5' Cap Modified Guanine nucleotide added to the 5' end. Protects mRNA from digestive enzymes. Recognition sign for ribosome attachment. Poly-A Tail 150-200 Adenine nucleotides added to the 3' tail Protects mRNA from digestive enzymes. Aids in mRNA transport from nucleus. Let’s see Transcription in motion… http://www.hhmi.org/biointera ctive/media/DNAi_transcriptio n_vo2-lg.mov RNA Splicing Removal of non-protein coding regions of RNA. Coding regions are then spliced back together. Introns Intervening sequences. Removed from RNA. Exons Expressed sequences of RNA. Translated into AAs. Spliceosome Cut out Introns and join Exons together. Made of snRNA and snRNP. Result Ribozymes RNA molecules that act as enzymes. Are sometimes Intron RNA and cause splicing without a spliceosome. Introns - Function Left-over DNA (?) Way to lengthen genetic message. Old virus inserts (?) Way to create new proteins. Final RNA Transcript Alternative Splicing The RNA can be spliced into different mRNA’s. Each different mRNA produces a different polypeptide. Ex. – variable regions of antibodies. Another Example – inhibits apoptosis Bcl-XS – induces apoptosis Bcl-XL Two different and opposite effects!! DSCAM Gene Found in fruit flies Has 100 potential splicing sites. Could produce 38,000 different polypeptides Many of these polypeptides have been found Commentary Alternative Splicing is going to be a BIG topic in Biology. About 60% of genes are estimated to have alternative splicing sites. One gene does not equal one polypeptide. Translation Process by which a cell interprets a genetic message and builds a polypeptide. Materials Required tRNA Ribosomes mRNA Transfer RNA = tRNA Made by transcription. About 80 nucleotides long. Carries AA for polypeptide synthesis. Structure of tRNA Has double stranded regions and 3 loops. AA attachment site at the 3' end. 1 loop serves as the Anticodon. Anticodon Region of tRNA that base pairs to mRNA codon. Usually is a compliment to the mRNA bases, so reads the same as the DNA codon. Example DNA - GAC mRNA - CUG tRNA anticodon - GAC Comment "Wobble" effect allows for 45 types of tRNA instead of 61. Reason - in the third position, U can pair with A or G. Inosine (I), a modified base in the third position can pair with U, C, or A. Importance Allows for fewer types of tRNA. Allows some mistakes to code for the same AA which gives exactly the same polypeptide. Aminoacyl-tRNA Synthetases Family of Enzymes. Add AAs to tRNAs. Active site fits 1AA and 1 type of tRNA. Uses a “secondary genetic” code to load the correct AA to each tRNA. Ribosomes Two subunits made in the nucleolus. Made of rRNA (60%)and protein (40%). rRNA is the most abundant type of RNA in a cell. Large subunit Proteins rRNA Both sununits Large Subunit Has 3 sites for tRNA. P site: Peptidyl-tRNA site carries the growing polypeptide chain. A site: Aminoacyl-tRNA site holds the tRNA carrying the next AA to be added. E site: Exit site Translation Steps 1. Initiation 2. Elongation 3. Termination Initiation Brings together: mRNA A tRNA carrying the 1st AA 2 subunits of the ribosome Initiation Steps: 1. Small subunit binds to the mRNA. 2. Initiator tRNA (Met, AUG) binds to mRNA. 3. Large subunit binds to mRNA. Initiator tRNA is in the P-site Initiation Requires other proteins called "Initiation Factors”. GTP used as energy source. Elongation Steps: 1. Codon Recognition 2. Peptide Bond Formation 3. Translocation Codon Recognition tRNA anticodon matched to mRNA codon in the A site. Peptide Bond Formation A peptide bond is formed between the new AA and the polypeptide chain in the P-site. Bond formation is by rRNA acting as a ribozyme After bond formation The polypeptide is now transferred from the tRNA in the P-site to the tRNA in the A-site. Translocation tRNA in P-site is released. Ribosome advances 1 codon, 5’ 3’. tRNA in A-site is now in the P-site. Process repeats with the next codon. Comment Elongation takes 60 milliseconds for each AA added. Termination Triggered by stop codons. Release factor binds in the A-site instead of a tRNA. H2O is added instead of AA, freeing the polypeptide. Ribosome separates. Let’s see Translation in motion… http://www.hhmi.org/biointera ctive/media/DNAi_translation _vo2-lg.mov Polyribosomes Cluster of ribosomes all reading the same mRNA. Another way to make multiple copies of a protein. Prokaryotes Comment Polypeptide usually needs to be modified before it becomes functional. Examples Sugars, lipids, phosphate groups added. Some AAs removed. Protein may be cleaved. Join polypeptides together (Quaternary Structure). Signal Hypothesis “Clue” on the growing polypeptide that causes ribosome to attach to ER. All ribosomes are “free” ribosomes unless clued by the polypeptide to attach to the ER. Result Protein is made directly into the ER . Protein targeted to desired location (e.g. secreted protein). “Clue” (the first 20 AAs are removed by processing). Mutations Changes in the genetic makeup of a cell. May be at chromosome or DNA level Chromosome Alterations Deletions Duplications Inversions Translocations General Result Loss of genetic information. Position effects: a gene's expression is influenced by its location to other genes. Evidence of Translocation Translocations Cri Du Chat Syndrome Part of p arm of #5 has been deleted. Good survival. Severe mental retardation. Small sized heads common. Philadelphia Chromosome An abnormal chromosome produced by a translocation of portions of chromosomes 9 and 22. Causes chronic myeloid leukemia. Mutation types - Cells cells or body cells – not inherited Germ Cells or gametes inherited Somatic DNA or Point Mutations Changes in one or a few nucleotides in the genetic code. Effects - none to fatal. Types of Point Mutations 1. Base-Pair Substitutions 2. Insertions 3. Deletions Base-Pair Substitution The replacement of 1 pair of nucleotides by another pair. Sickle Cell Anemia Lets see how this mutation will affect the cell… http://www.hhmi.org/biointera ctive/media/DNAi_sicklecelllg.mov Types of Substitutions 1. Missense - altered codons, still code for AAs but not the right ones 2. Nonsense - changed codon becomes a stop codon. Question? What will the "Wobble" Effect have on Missense? If the 3rd base is changed, the AA may still be the same and the mutation is “silent”. Missense Effect Can be none to fatal depending on where the AA was in the protein. Ex: if in an active site - major effect. If in another part of the enzyme - no effect. Nonsense Effect Stops protein synthesis. Leads to nonfunctional proteins unless the mutation was near the very end of the polypeptide. Sense Mutations The changing of a stop codon to a reading codon. Result - longer polypeptides which may not be functional. Ex. “heavy” hemoglobin Insertions & Deletions The addition or loss of a base in the DNA. Cause frame shifts and extensive missense, nonsense or sense mutations. Question? Loss of 3 nucleotides is often not a problem. Why? Because the loss of a 3 bases or one codon restores the reading frame and the protein may still be able to function. Mutagenesis Process of causing mutations or changes in the DNA. Mutagens Materials that cause DNA changes. 1. Radiation ex: UV light, X-rays 2. Chemicals ex: 5-bromouracil Spontaneous Mutations Random errors during DNA replication. Comment Any material that can chemically bond to DNA, or is chemically similar to the nitrogen bases, will often be a very strong mutagen. Summary Know Beadle and Tatum. Know the central dogma. Be able to “read” the genetic code. Be able to describe the events of transcription and translation. Summary Be able to discuss RNA and protein processing. Be able to describe and discuss mutations.